scholarly journals Molecular cloning and characterization of a lipoxygenase gene (CsLOX1) from cucumber

2020 ◽  
Vol 48 (4) ◽  
pp. 1832-1844
Author(s):  
Yong ZHOU ◽  
Mingyuan XU ◽  
Wei LAI ◽  
Lianghai CHEN ◽  
Yingui YANG ◽  
...  
Keyword(s):  
Defense Responses ◽  
Regulatory Elements ◽  
Heme Iron ◽  
Male Sterile ◽  
Multiple Sequence ◽  
Conserved Residues ◽  
Cis Acting ◽  
Non Heme Iron ◽  
Lipoxygenase Gene

Lipoxygenases (LOXs) are non-heme iron enzymes that play crucial roles in many developmental processes during plant life, and defense responses against biotic and abiotic stresses. In this study, a lipoxygenase gene (CsLOX1) was cloned and characterized from cucumber (Cucumis sativus). The coding sequence (CDS) of CsLOX1 was 741 bp, and encoded an 878 amino-acid residue protein, which was predicted to be located in the cytoplasm. CsLOX1 contained the conserved LH2/PLAT and lipoxygenase domains, as well as the representative 38 amino acids motif [His-(X)4-His-(X)4-His-(X)17-His-(X)8-His]. Multiple sequence alignment and phylogenetic analysis indicated that CsLOX1 was closely related to other dicot 9-LOXs and posesess the essential conserved residues involved in the binding of the iron atom. Promoter analysis suggested that several development-, stress-, and hormone-related cis-acting regulatory elements were present in the promoter region of CsLOX1. The function of CsLOX1 was assessed by overexpression it in Arabidopsis, and the transgenic plants were male sterile and displayed obviously increased floral shoots. These results provide some clues for revealing the biological roles of CsLOX1 in cucumber.

Activation of dioxygen by a mononuclear non-heme iron complex: characterization of a FeIII(OOH) intermediate

2010 ◽  
Vol 39 (6) ◽  
pp. 1630-1634 ◽  
Author(s):  
Marlène Martinho ◽  
Guillaume Blain ◽  
Frédéric Banse
Keyword(s):  
Iron Complex ◽  
Heme Iron ◽  
Non Heme Iron ◽  
Complex Characterization

scholarly journals Isolation, Cloning and Characterization of a Constitutive Plant from Potato Aquaporin Gene

2020 ◽  
Vol 63 (3) ◽  
pp. 169-178
Author(s):  
Hira Mubeen ◽  
Rubab Zahra Naqvi ◽  
Ammara Masood ◽  
Mushtaq A. Saleem ◽  
Aftab Bashir ◽  
...  
Keyword(s):  
Genomic Sequence ◽  
Crop Improvement ◽  
Regulatory Elements ◽  
Gus Expression ◽  
Cis Acting ◽  
Transcription Start Sites ◽  
Expression Studies ◽  
Aquaporin Gene ◽  
Highly Expressed Genes

Plasma membrane intrinsic proteins (PIP1) are the most common integral membrane proteins belong to a larger family of intrinsic aquaporin proteins. They are member of aquaporin gene family and have gained importance as highly expressed genes in plants. In this study, the promoter of aquaporin PIP1 gene was identified, analyzed and retrieved from high throughput genomic sequence (HTGS) database. The cis-acting regulatory elements, transcription start sites and transcription factor binding sites of selected promoter were identified through different bio-informatics tools. Many light responsive, phytohormone, stress and defense related cis-regulatory elements were detected in PIP1 promoter region indicating its role as a constitutive promoter. The PIP1 promoter was isolated from Solanum tuberosum. It was initially cloned in TA vector (pTZ57R/T) and later transferred to plant expression binary vectors, pGR1 and pGA482 for transient and stable expression studies in tobacco. The GUS expression results of PIP1 promoter in different tobacco tissues showed its functional importance in regulating gene expression in a constitutive manner. Further, it was concluded that the PIP1 aquaporin promoter is constitutively expressed with a strength equivalent to CaMV 2x35S promoter. These findings indicated the significance of isolated promoter for genetic engineering of plants for crop improvement.  

scholarly journals Structural characterization of a non-heme iron active site in zeolites that hydroxylates methane

2018 ◽  
Vol 115 (18) ◽  
pp. 4565-4570 ◽  
Author(s):  
Benjamin E. R. Snyder ◽  
Lars H. Böttger ◽  
Max L. Bols ◽  
James J. Yan ◽  
Hannah M. Rhoda ◽  
...  
Keyword(s):  
Active Site ◽  
Density Functional ◽  
Density Functional Theory Calculations ◽  
Heme Iron ◽  
Zeolite Lattice ◽  
Non Heme Iron ◽  
Bonding Interaction ◽  
Lattice Density ◽  
X Ray Absorption

Iron-containing zeolites exhibit unprecedented reactivity in the low-temperature hydroxylation of methane to form methanol. Reactivity occurs at a mononuclear ferrous active site, α-Fe(II), that is activated by N2O to form the reactive intermediate α-O. This has been defined as an Fe(IV)=O species. Using nuclear resonance vibrational spectroscopy coupled to X-ray absorption spectroscopy, we probe the bonding interaction between the iron center, its zeolite lattice-derived ligands, and the reactive oxygen. α-O is found to contain an unusually strong Fe(IV)=O bond resulting from a constrained coordination geometry enforced by the zeolite lattice. Density functional theory calculations clarify how the experimentally determined geometric structure of the active site leads to an electronic structure that is highly activated to perform H-atom abstraction.

scholarly journals Rapid purification of rabbit reticulocyte lipoxygenase for electron paramagnetic spectroscopy characterization of the non-heme iron

Lipids ◽  
1993 ◽  
Vol 28 (3) ◽  
pp. 241-244 ◽  
Author(s):  
Richard T. Carroll ◽  
Jessica Muller ◽  
Jennifer Grimm ◽  
William R. Dunham ◽  
Richard H. Sands ◽  
...  
Keyword(s):  
Heme Iron ◽  
Non Heme Iron ◽  
Rapid Purification ◽  
Electron Paramagnetic Spectroscopy ◽  
Electron Paramagnetic
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