scholarly journals Comparative study on in vitro micropropagation response of seven globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars: open-pollinated cultivars vs F1 hybrids

2020 ◽  
Vol 48 (3) ◽  
pp. 1210-1220
Author(s):  
Tugce OZSAN ◽  
Ahmet N. ONUS
Keyword(s):  
In Vitro Rooting ◽  
Cultivar Differences ◽  
F1 Hybrids ◽  
Globe Artichoke ◽  
Ms Medium ◽  
Cynara Cardunculus ◽  
Mass Propagation ◽  
F1 Hybrid ◽  
In Vitro Micropropagation

Globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] growing has gained commercial importance in recent years due to its consumption as food. It has also started to attract attention in pharmaceutics. Due to globe artichoke’s stated importance, growers need large amount of pathogen-free, healthy starting materials for production. Stated material will maximize the yield while minimizing the costs. Hybrid cultivars have uniform in height and maturity and could be harvested concurrently; on the other hand, an open-pollinated cultivar would have useful potential that could be smoothly produced locally at a lower cost. In vitro micropropagation enabling these goals as it serves large scale, fast, reliable and realistic alternative method to classic propagation via offshoots. The aim of the present study was to comparatively evaluate the micropropagation efficiency of two important local open-pollinated (OP) cultivars (‘Bayrampaşa’, ‘Sakız’) and five F1 hybrid cultivars (‘Olympus’, ‘Madrigal’, ‘Sambo’, ‘Green Globe’, ‘Imparator’), on the basis of total subcultures they were subjected to. Various plant growth regulators at various combinations were assessed for in vitro micropropagation and subsequent in vitro rooting. 3/4 basic MS medium supplemented with 0.05 mg  L-1 BA + 0.005 mg L-1 IBA was determined as the best media combination for in vitro micropropagation, while 10.0 mg L-1 IAA + 1.0 g L-1 activated charcoal adding to 1/2 basic MS medium had positive effects on in vitro rooting. According to results, the micropropagation efficiency varied based on cultivar differences and number of subcultures regardless of being OP or F1 hybrid. The present study demonstrated that in vitro propagation of globe artichoke could be a valuable process for assessing mass propagation regardless of using F1 or OP cultivars. Considering the OP cultivars are cheap in terms of price in a comparison to F1 hybrid cultivars, OP cultivars could be also recommended to be used for in vitro mass propagation. 

scholarly journals Effect of Sucrose Concentrations and Incubation Periods on in Vitro Rooting of Moris Pineapple (Ananas comosus)

2019 ◽  
Vol 34 (4) ◽  
pp. 230-242
Author(s):  
Abdelhamid M Hamad
Keyword(s):  
Incubation Period ◽  
In Vitro Rooting ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Incubation Periods ◽  
Half Strength

The effect of 6 sucrose concentrations (5, 10, 15, 20, 25, 30 g/l) over 4 incubation periods (30, 45, 60, 75 days) on in vitro rooting of Moris pineapple cultured in liquid half strength MS medium enriched with IBA at 2.0 mg/l was investigated. At all incubation periods, all shoots in medium enriched with sucrose at 5 g/l failed to root, and no roots formed within the first 30 days in medium enriched with sucrose at 10 g/l. After 30 days of incubation, the highest rooting percentage (66 %), tallest plantlets (23 mm tall), highest (3.4 roots) and longest (5.3 mm) root per shoot were obtained in medium enriched with sucrose at 25, 10, 15, 15 g/l respectively, while after 45 days, the highest of all rooting aspects (75 %, 32.3 mm tall, 3.7 roots, 7 mm long), were obtained in medium enriched with sucrose at 15 g/l. After 60 days, the highest rooting percentage (91.7 %) and tallest plantlets (36.7 mm tall) were obtained in medium enriched with sucrose at 20 g/l while highest roots per shoot (3.7 roots) and longest root (10.7 mm) were obtained in medium enriched with sucrose at 15 g/l. After 75 days, all shoots rooted (100 %) in medium enriched with sucrose at 10 and 20 g/l, while sucrose at 25 g/l resulted in tallest plantlets (46.3 mm tall) and at 20 g/l resulted in highest (4.7 roots) and longest roots (27.3 mm). At each incubation period, there was a different optimum sucrose enrichment for different rooting parameters.

scholarly journals Research on in vitro micropropagation of Lilium brownii F.E. Brown

2016 ◽  
Vol 14 (1) ◽  
pp. 121-129
Author(s):  
Vũ Hoài Sâm ◽  
Bùi Đức Quỳnh ◽  
Nguyễn Thị Hương ◽  
Nguyễn Văn Khiêm
Keyword(s):  
Subtropical Climate ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
Medicinal Species ◽  
Surface Sterilization ◽  
The North ◽  
In Vitro Micropropagation ◽  
Half Strength ◽  
Over Exploitation

Lilium brownii Brown belonging Lilium genus and Liliaceae family is well-known as a popular medicinal species, as well as food source and beautiful ornamental flowers. The specie has unique and ornamental floral characteristics such as light and elegant fragrance and perianth color rapidly changing from yellowish cream to white during anthesis. In traditional medicine, it is used for treatment cough, sedation diuretic, bronchitis... In nature, it can be found in subtropical climate moutainous areas in the North such as Sa Pa, Bat Xat, Mu Cang Chai; Sin Ho and Phong Tho, Quang Ba and Dong Van. In recent years, this species has been listed in the Red List for medicinal plants in Vietnam due to over-exploitation. The only effective strategy for sustaible conservation this species is in vitro micropropagation. In this study, in vitro plant regeneration and micropropagation of L. brownii was established from bubles and stem nodes. After surface sterilization with 0.1% HgCl­2 in 10 minutes, healthy young shoots were obtained from initial bubles and stem nodes on MS medium supplemented with 0.5 mg/l BAP or 0.5 mg/l NAA, respectively.  Bulblets also were formed from young shoot on MS supplemented with 0.5 mg/l NAA. The highest number of 4.5 bulblets per an explant was recorded from longitude-divided bubbles on MS medium containing 0.5 mg/l NAA and 0.2 mg/l BAP after 60 days in culture. The regererated plants produced quality roots on half strength MS supplemented with the combination of 1.0 mg/ l NAA and 0.2 mg / l BAP. More than 90% of rooted plants in vitro were survival on artificial soil TN1 in the nursery.

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

The roles of pyruvate, lactate and glucose during preimplantation development of embryos from F1 hybrid mice in vitro

Development ◽  
1991 ◽  
Vol 112 (1) ◽  
pp. 99-105 ◽  
Author(s):  
J.J. Brown ◽  
D.G. Whittingham
Keyword(s):  
Inbred Mouse ◽  
Blastocyst Stage ◽  
Preimplantation Development ◽  
F1 Hybrids ◽  
Mouse Strains ◽  
F1 Hybrid ◽  
Morula Stage ◽  
Lactate And Pyruvate ◽  
Hybrid Mice

Embryos of certain inbred mouse strains, and their F1 hybrids, are able to develop from the 1-cell to blastocyst stage in simple chemically defined media containing lactate (L), pyruvate (P) and glucose (G). The individual roles of these substrates in supporting complete preimplantation development in vitro was examined with 1-cell F2 embryos from B6CBF1 hybrid mice. Embryos collected between 26 and 27 h post hCG were cultured in medium containing L, P, LP or LPG. After 50 h in culture, the proportions developing to the morula stage were 1%, 83%, 94% and 100%, respectively. In combination, lactate and pyruvate appeared to act synergistically and both the rate and level of development to the morula stage were unaffected by the absence of glucose. After a further 46 h in culture, only the embryos grown in the presence of glucose developed into blastocysts. In LP medium, embryos arrested at the compacted morula stage late on day 3 of development. As culture continued in the absence of glucose, embryos decompacted (approximately 82 h post hCG) and subsequently degenerated. Exposure to medium containing glucose for the first, second or third 24 h period in culture was sufficient to support the morula-to-blastocyst transition. Glucose still supported this transition when embryos were transferred to LPG medium 3 h after the completion of compaction (76 h post hCG), but was ineffective 6 h later (82 h post hCG) once decompaction had commenced. We conclude that lactate and pyruvate together are able to support normal development of 1-cell F2 embryos to the morula stage in vitro, but that glucose is an essential component of the culture medium for development to the blastocyst stage.

IN VITRO RESPONSE BY Terminalia arjuna GENOTYPES DURING MICROPROPAGATION

2021 ◽  
Vol 9 (1) ◽  
pp. 44-50
Author(s):  
Meena Choudhary ◽  
◽  
Ashok Gehlot ◽  
Sarita Arya ◽  
Inder Dev Arya ◽  
...  
Keyword(s):  
Shoot Proliferation ◽  
In Vitro Rooting ◽  
Terminalia Arjuna ◽  
Ms Medium ◽  
Demand And Supply ◽  
Half Strength ◽  
Modified Ms Medium ◽  
In Vitro Response ◽  
Different Doses

Terminalia arjuna is an important tree of the medicinal and sericulture industry, commonly known as Arjun. It’s bark is rich in secondary metabolites makes this plant highly valuable in medicine industry to treat cardiovascular disease. Overexploitation due to high demand in medicine, low seed germination, limitations of the conventional method of propagation push this plant towards being endangered. To conserve germplasm of such tree species and meet the requirement in medicinal industry, some non-conventional propagation method like micropropagation has been developed. The present work highlighted the effect of three genotypes (G-1, G-2, and G-3) on tissue culture of T. arjuna situated at Jodhpur, Rajasthan, India. In vitro shoot proliferation was achieved on a modified MS medium enriched with BAP + additives. Among the tested genotypes, Genotype -1 showed maximum bud break response (100%) followed by G-3 (93.33 %) and G-2 (86.66%). Further multiplication of these shoots on modified MS medium containing BAP + NAA + additives gave 11.38±0.26 (G-1), 9.44±0.21 (G-2) and 10.22±0.32 (G-3) shoots. In vitro rooting was done by pulse treatment with IBA for 10 min prior to transfer on hormone free half strength MS medium containing 0.1% activated charcoal. Maximum in vitro rooting was obtained in G-1 (80%) followed by G-3 (71.11%) and G-2 (68.88%). In the present study, it was observed that optimum growth in all three genotypes required different doses of Plant Growth Regulator. Thus, by identifying and multiplying the best performing genotypes the gap between demand and supply of such medicinal plant can be fulfilled.

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

scholarly journals Effect of phytohormones on shoot apex and leaf explants of Withania somnifera (Ashwagandha)

2016 ◽  
Vol 8 (1) ◽  
pp. 412-415 ◽  
Author(s):  
Archana Rani ◽  
M. Kumar ◽  
Sanjeev Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Apex ◽  
Callus Induction ◽  
Withania Somnifera ◽  
Leaf Explants ◽  
Ms Medium ◽  
Mass Propagation ◽  
Best Response ◽  
Dichlorophenoxy Acetic Acid

An efficient protocol for callus induction of Withania somnifera through in vitro culture of shoot apex and leaf explant was standardized. Of the various combinations of phytohormones evaluated, MS media supplemented with 6-furfuryl aminopurine (KIN) 0.5 mg/l + 2,4-dichlorophenoxy acetic acid (2, 4-D) 2.0 mg/l was found to be bestfor mean callus induction (86%) in leaf explants after 6 weeks of culture and in case of shoot apex expant the best response and growth of callusing was observed on MS medium supplemented with 2,4-D 1.0 mg/l + BAP 2.0 mg/l (77%).The response of callus growth increases gradually with the reductions in concentration of KIN in culturemedium of both the explants. This protocol might be used in further research for mass propagation of W. somnifera via indirect regeneration methods.

scholarly journals Kultur in vitro pisang Kepok Merah (Musa paradisiaca) untuk mikropropagasi cepat

2017 ◽  
Vol 84 (2) ◽  
Author(s):  
Efah FITRAMALA ◽  
Eva KHAERUNNISA ◽  
Nina Ratna Djuita Ratna DJUITA ◽  
Hadi SUNARSO ◽  
Diah RATNADEWI
Keyword(s):  
Woody Plant ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Musa Paradisiaca ◽  
In Vitro Micropropagation ◽  
The Media ◽  
B Complex Vitamins ◽  
Initiation Stage ◽  
Planting Materials

AbstractBanana (Musa paradisiaca L) cv. Kepok Merah has a high commercial value as it is used in food industries such as banana chip. Besides, Kepok Merah contains high B-complex vitamins that serve in energy metabolism and are important in the development of infant brain. The establishment of industrial plantations of this plant has been restricted by the lack of planting materials. This research aimed at ameliorating the capacity of plantlets multiplication up to rooting of this banana in a rapid way through in vitro multiplication techniques. Murashige and Skoog (MS) and Woody Plant (WP) were used as the basic media. For the initiation stage, the media was fortified with 0.2 mg/L IAA and two levels of BA at 3 and 5 mg/L.  For shoot multiplication, the concentrations of IAA as well as BA were increased. For rooting, 1 mg/L NAA or IBA was applied. The observations demonstrated that for shoots initiation, both basic media performed good results when enriched with 0.2 mg/L IAA and 5 mg/L BA. The highest rate of shoots multiplication at 6 – 17 shoots per explant, was obtained on MS medium added with 0.5 mg/L IAA and 5 mg/L BA.  NAA at 1 mg/L in MS medium produced more rooted plantlets, 3 – 16 roots per plantlet, than those of other treatments. Keywords: Musa paradisiaca cv. Kepok Merah, in vitro micropropagation, scalps.AbstrakPisang (Musa paradisiaca L.) kultivar Kepok Merah memiliki nilai komersial yang cukup tinggi yaitu sebagai bahan dalam industri pembuatan keripik pisang. Selain itu, pisang Kepok Merah memiliki kandungan vitamin B kompleks cukup tinggi untuk membantu produksi energi dan pembentukan sel-sel otak pada bayi. Pertanaman pisang ini dalam skala industri terkendala oleh kurangnya ketersediaan sumber benih. Teknik kultur jaringan diharapkan dapat menghasilkan benih secara massal dalam waktu yang relatif singkat. Tujuan dari penelitian ini adalah meningkatkan keberhasilan multiplikasi tunas in vitro hingga pengakaran tanaman pisang Kepok Merah secara cepat. Pada tahap inisiasi tunas digunakan media dasar Murashige and Skoog (MS) dan media Woody Plant (WP); ke dalam media dasar tersebut ditambahkan IAA 0,2 mg/Ldan 2 taraf BA yaitu 3 dan 5 mg/L. Multiplikasi tunas dilakukan pada media dasar yang sama namun dengan taraf konsentrasi IAA serta BA yang ditingkatkan. Tahap perakaran menggunakan media dasar MS dan WP dengan auksin NAA 1 mg/L atau IBA 1 mg/L. Hasil penelitian menunjukkan bahwa untuk inisiasi tunas, media MS dan WP yang diperkaya dengan IAA 0,2 mg/L dan BA 5 mg/L   sama baiknya. Untuk multiplikasi tunas, media MS dengan IAA 0.5 mg/L   yang dikombinasikan dengan BA 5 mg/L   memberikan jumlah tunas paling banyak, yaitu 6 – 17 tunas per eksplan, dan pertumbuhannyapun lebih baik. Pemberian NAA 1 mg/L   pada media MS dapat memberikan lebih banyak tunas yang berakar, dengan jumlah akar 3 – 16 per planlet.  Kata kunci: Musa paradisiaca cv. Kepok Merah, mikropropagasi in vitro, nodul meristematik

scholarly journals Salinity-Induced Alterations in the Growth, Membranes and Osmolytes of Musa spp. cv. Caipira (AAA) During Micropropagation

2019 ◽  
Vol 11 (6) ◽  
pp. 171
Author(s):  
Yuri Lima Melo ◽  
Isabele Aragão Gomes Trindade ◽  
Monique Cristina Simão Lopes ◽  
Cibelley Vanúcia Santana Dantas ◽  
Josemir Moura Maia ◽  
...  
Keyword(s):  
Survival Rate ◽  
Plant Height ◽  
Exposure Time ◽  
Root Length ◽  
Growth Traits ◽  
In Vitro Rooting ◽  
Ms Medium ◽  
Musa Spp ◽  
Number Of Leaves

The aim of this study was to determine the concentration and exposure time to NaCl suitable for the micropropagation of banana, through the analysis of growth traits. Banana propagules were inoculated in MS medium with different concentrations of NaCl (0; 50; 75 and 100 mM) for 120 days (multiplication and rooting, 60 days each), with monthly subcultures. These propagules were measured for plant height, number of leaves, sprouting rate, average number of formed propagules, rooting rate, root length and survival rate. After 30 days, NaCl reduced sprouting rate at multiplication; the number of leaves, rooting rate and root length in rooting; and the height and propagules number in both phases. After 60 days, the NaCl affected the sprouting rate and propagules number in the multiplication; length of root in rooting; and the height and number of leaves in both phases. After 120 days, the reduction in the survival rate was proportional to the increase of NaCl in the medium. Thus, it is concluded that NaCl reduces most of the growth traits and the treatments with 75 and 100 mM NaCl affected multiplication and in vitro rooting more intensely.

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