scholarly journals Alleviation of Salt Stress in Seedlings of Black Glutinous Rice by Seed Priming with Spermidine and Gibberellic Acid

2014 ◽  
Vol 42 (2) ◽  
pp. 405-413 ◽  
Author(s):  
Sumitahnun CHUNTHABUREE ◽  
Jirawat SANITCHON ◽  
Wattana PATTANAGUL ◽  
Piyada THEERAKULPISUT
Keyword(s):  
Salt Stress ◽  
Gibberellic Acid ◽  
Oryza Sativa L ◽  
Seed Priming ◽  
Physiological Data ◽  
Chlorophyll Contents ◽  
Physiological Indicators ◽  
Phenolic Contents ◽  
Glutinous Rice ◽  
Hydroponically Grown

This study was carried out to elucidate the spermidine (Spd) and gibberellic acid (GA3) priming-induced physiological and biochemical changes responsible for induction of salinity tolerance in two rice (Oryza sativa L.) cultivars, namely ‘Niewdam Gs. no. 00621’ (salt tolerant) and ‘KKU-LLR-039’ (salt sensitive). The seeds of the two cultivars were primed separately with distilled water, 1 mM Spd or 0.43 mM GA3. Primed seeds were germinated and the resultant seedlings were hydroponically grown for 14 days before being exposed to salinity stress (150 mM NaCl) for 10 days. Seed priming with Spd or GA3 slightly improved salt-induced reductions in growth, anthocyanin and chlorophyll contents of the seedlings. Salt stress induced pronounced increases in Na+/K+ ratio, proline and H2O2 contents, particularly in the sensitive cultivar. The levels of these salt-sensitivity physiological indicators tended to be mitigated by priming with Spd and GA3. Salt-stressed seedlings grown from seeds primed with these growth regulators also possessed higher phenolic contents and greater antioxidant capacity than the control seedlings. Based on all growth and physiological data, Spd tended to be more effective than A3 in improving salt tolerance in both rice cultivars.

scholarly journals Alleviation of Salt Stress in Seedlings of Black Glutinous Rice by Seed Priming with Spermidine and Gibberellic Acid

2014 ◽  
Vol 42 (2) ◽  
Author(s):  
Sumitahnun CHUNTHABUREE ◽  
Jirawat SANITCHON ◽  
Wattana PATTANAGUL ◽  
Piyada THEERAKULPISUT
Keyword(s):  
Salt Stress ◽  
Gibberellic Acid ◽  
Seed Priming ◽  
Glutinous Rice

scholarly journals Seed Priming with Potassium Nitrate and Gibberellic Acid Enhances the Performance of Dry Direct Seeded Rice (Oryza sativa L.) in North-Western India

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 849
Author(s):  
Buta Singh Dhillon ◽  
Virender Kumar ◽  
Pardeep Sagwal ◽  
Navjyot Kaur ◽  
Gurjit Singh Mangat ◽  
...  
Keyword(s):  
Gibberellic Acid ◽  
Grain Yield ◽  
Oryza Sativa L ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Growth And Yield ◽  
Western India ◽  
Yield Attributes ◽  
Crop Establishment ◽  
Direct Seeded

Poor early growth and uneven crop establishment are reported as the major bottlenecks in wide-scale adoption and optimal yield realization of dry direct-seeded rice (DSR). Seed priming can potentially help overcome these problems in DSR. Therefore, laboratory and field studies were conducted at Punjab Agricultural University, Ludhiana, India, during kharif/wet-season 2018 and 2019 to evaluate the effect of different priming techniques on germination, establishment, growth, and grain yield of rice under DSR conditions. The following priming treatments were evaluated: dry non-primed seed (control), hydropriming with distilled water, halopriming with 2.0% potassium nitrate, hormopriming with 50 ppm gibberellic acid (GA3), and osmopriming with polyethylene glycol (PEG)(−0.6 MPa), each with 12 and 24 h priming duration. In 2019, priming treatments were tested under two DSR establishment methods—conventional DSR (sowing in dry soil followed by irrigation) and soil mulch DSR (locally known as vattar DSR) (sowing in moist soil after pre-sowing irrigation), whereas in 2018, priming treatments were evaluated under conventional DSR only. In both years, halopriming and hormopriming resulted in a 7–11% increase in rice yields compared to non-primed dry seed (control). Osmopriming resulted in a 4% yield increase compared to control in 2018 but not in 2019. The higher yields in halopriming and hormopriming were attributed to higher and rapid germination/crop emergence, better root growth, and improvement in yield attributes. Priming effect on crop emergence, growth, and yield did not differ by DSR establishment methods and duration of priming. Conventional DSR and soil mulch DSR did not differ in grain yield, whereas they differed in crop emergence, growth, and yield attributes. These results suggest that halopriming with 2.0% potassium nitrate and hormopriming with 50 ppm GA3 has good potential to improve crop establishment and yield of rice in both conventional and soil mulch DSR systems.

Alleviation of salt stress using gibberellic acid in Chinese cabbage

2012 ◽  
Vol 60 (4) ◽  
pp. 345-355 ◽  
Author(s):  
M. Jamil ◽  
M. Ashraf ◽  
E. Rha
Keyword(s):  
Salt Stress ◽  
Gibberellic Acid ◽  
Chinese Cabbage ◽  
Germination Rate ◽  
Harmful Effect ◽  
Seed Priming ◽  
Nacl Stress ◽  
Growth And Yield ◽  
Distilled Water ◽  
Membrane Injury

Salinity reduces plant growth and yield by affecting morphological and physiological processes. To alleviate the harmful effects of salt stress various approaches involving plant hormones are used. In this study several parameters involving the measurement of cell membrane injury were used to observe whether stress tolerance could be enhanced in Chinese cabbage (B. oleracea capitata L. Chinensis group) by soaking the seeds for 10 h in distilled water (control), or in 100, 150 or 200 mg l−1 gibberellic acid (GA3). The NaCl concentrations were 0 (control), 50, 100 and 150 mM. Seed treated with GA3 showed increased water uptake and decreased electrolyte leakage as compared to that of distilled water-primed seeds even 24 h after soaking under control conditions. Seed priming with GA3 increased the final germination and the germination rate (1/t50, where t50 is the time to 50% germination) under salt stress conditions. Seed priming also alleviated the harmful effect of salt stress on cabbage in terms of fresh and dry weights. Leaf area was higher in plants raised from seeds primed with the higher GA3 concentrations as compared with those raised from seeds treated with distilled water under control conditions (without NaCl) or at 50 mM NaCl stress. The chlorophyll content increased with the NaCl concentration, especially in plants grown from seeds primed with GA3. Plants grown from GA3-primed seeds also suffered lower cellular injury both under control conditions and under NaCl stress.

scholarly journals Changes in growth, biochemical components and antioxidant genes expression in rice seedling (Oryza sativa L.) cultivar ‘IR64’ under salt stress

2019 ◽  
Author(s):  
M. Pharmawati ◽  
I.M.A.S. Wijaya
Keyword(s):  
Salt Stress ◽  
Salinity Stress ◽  
Oryza Sativa L ◽  
Rice Seedling ◽  
Antioxidant Genes ◽  
Shoot Height ◽  
Hydroponic System ◽  
Chlorophyll Contents ◽  
Transcription System ◽  
Genes Expression

One of abiotic stresses that affects rice growth is salinity. Plant must develop adaptation process which includes morphological, biochemical and molecular changes. This research aimed to evaluate morpho-biochemical and molecular responses of rice ‘IR64’ to several levels of salinity stress at seedling stage. Seedlings of ‘IR64’ were grown in a hydroponic system and treated with different levels of salinity stress (4dSm-1, 6dSm-1, and 12dSm-1) for seven days. Responses were recorded on the final day of salt treatments. Gene expression analyses were done by semi-quantitative RT-PCR. RNA was extracted using RNase plant mini kit (Qiagen) and cDNA was synthesized using GoSript™ Reverse Transcription System (Promega). Results showed that shoot height and fresh weight decreased under salt stress. At plants treated with salt, the chlorophyll contents were lower than that of control plants, while MDA levels were higher in salt treated plants. Semi-quantitative PCR for MnSOD1and cCu/ZnSOD1 revealed that MnSOD1 and cCu/ZnSOD1expressions increased under salt stress which indicated oxidative stress defence, with the highest expression at 4dSm-1and 6dSm-1 treatment, respectively.

scholarly journals Exogenously Applied Gibberellic Acid Enhances Growth and Salinity Stress Tolerance of Maize through Modulating the Morpho-Physiological, Biochemical and Molecular Attributes

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1005
Author(s):  
Kashif Shahzad ◽  
Sadam Hussain ◽  
Muhammad Arfan ◽  
Saddam Hussain ◽  
Ejaz Ahmad Waraich ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Gibberellic Acid ◽  
Growth And Development ◽  
Foliar Spray ◽  
Seed Priming ◽  
Ion Concentration ◽  
Genes Expression ◽  
Antioxidant Enzymes Activities

Soil salinity is the major limiting factor restricting plant growth and development. Little is known about the comparative and combined effects of gibberellic acid (GA3) seed priming and foliar application on maize under salt stress. The current study determined the impact of different application methods of GA3 on morpho-physiological, biochemical and molecular responses of maize seedlings under three salinity stress treatments (no salinity, moderate salinity-6 dS m−1, and severe salinity-12 dS m−1). The GA3 treatments consisted of control, hydro-priming (HP), water foliar spray (WFS), HP + WFS, seed priming with GA3 (GA3P, 100 mg L−1), foliar spray with GA3 (GA3FS, 100ppm) and GA3P + GA3FS. Salt stress particularly at 12 dS m−1 reduced the length of shoots and roots, fresh and dry weights, chlorophyll, and carotenoid contents, K+ ion accumulation and activities of antioxidant enzymes, while enhanced the oxidative damage and accumulation of the Na+ ion in maize plants. Nevertheless, the application of GA3 improved maize growth, reduced oxidative stress, and increased the antioxidant enzymes activities, antioxidant genes expression, and K+ ion concentration under salt stress. Compared with control, the GA3P + GA3FS recorded the highest increase in roots and shoots length (19-37%), roots fresh and dry weights (31-43%), shoots fresh and dry weights (31-47%), chlorophyll content (21-70%), antioxidant enzymes activities (73.03-150.74%), total soluble protein (13.05%), K+ concentration (13-23%) and antioxidants genes expression levels under different salinity levels. This treatment also reduced the H2O2 content, and Na+ ion concentration. These results indicated that GA3P + GA3FS could be used as an effective tool for improving the maize growth and development, and reducing the oxidative stress in salt-contaminated soils.

High-density linkage mapping for agronomic and physiological traits of rice (Oryza sativa L.) under reproductive-stage salt stress

2021 ◽  
Vol 100 (2) ◽  
Author(s):  
Mostafa Ahmadizadeh ◽  
Nadali Babaeian-Jelodar ◽  
Ghasem Mohammadi-Nejad ◽  
Nadali Bagheri ◽  
Rakesh Kumar Singh
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Linkage Mapping ◽  
Oryza Sativa L ◽  
High Density ◽  
Reproductive Stage ◽  
Physiological Traits

scholarly journals Transcriptomic Analysis of Short-Term Salt-Stress Response in Mega Hybrid Rice Seedlings

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1328
Author(s):  
Noushin Jahan ◽  
Yang Lv ◽  
Mengqiu Song ◽  
Yu Zhang ◽  
Liangguang Shang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Molecular Mechanisms ◽  
Oryza Sativa L ◽  
Transcriptome Profiling ◽  
Bhlh Transcription Factor ◽  
F1 Hybrid ◽  
Salt Stress Response ◽  
Productivity Losses ◽  
Expression Of Genes ◽  
Trait Locus

Salinity is a major abiotic stressor that leads to productivity losses in rice (Oryza sativa L.). In this study, transcriptome profiling and heterosis-related genes were analyzed by ribonucleic acid sequencing (RNA-Seq) in seedlings of a mega rice hybrid, Liang-You-Pei-Jiu (LYP9), and its two parents 93–11 and Pei-ai64s (PA64s), under control and two different salinity levels, where we found 8292, 8037, and 631 salt-induced differentially expressed genes (DEGs), respectively. Heterosis-related DEGs were obtained higher after 14 days of salt treatment than after 7 days. There were 631 and 4237 salt-induced DEGs related to heterosis under 7-day and 14-day salt stresses, respectively. Gene functional classification showed the expression of genes involved in photosynthesis activity after 7-day stress treatment, and in metabolic and catabolic activity after 14 days. In addition, we correlated the concurrence of an expression of DEGs for the bHLH transcription factor and a shoot length/salinity-related quantitative trait locus qSL7 that we fine-mapped previously, providing a confirmed case of heterosis-related genes. This experiment reveals the transcriptomic divergence of the rice F1 hybrid and its parental lines under control and salt stress state, and enlightens about the significant molecular mechanisms developed over time in response to salt stress.

Silicon alleviates salt stress-induced potassium deficiency by promoting potassium uptake and translocation in rice (Oryza sativa L.)

2021 ◽  
Vol 258-259 ◽  
pp. 153379
Author(s):  
Guochao Yan ◽  
Xiaoping Fan ◽  
Wanning Zheng ◽  
Zixiang Gao ◽  
Chang Yin ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Oryza Sativa L ◽  
Potassium Deficiency ◽  
Potassium Uptake

scholarly journals Efficacy of KNO3, SiO2 and SA priming for improving emergence, seedling growth and antioxidant enzymes of rice (Oryza sativa), under drought

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lawan Gana Ali ◽  
Rosimah Nulit ◽  
Mohd Hafiz Ibrahim ◽  
Christina Yong Seok Yien
Keyword(s):  
Salicylic Acid ◽  
Seedling Growth ◽  
Antioxidant Activities ◽  
Soluble Sugar ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Total Soluble Protein ◽  
Rice Seedlings ◽  
Chlorophyll Contents ◽  
Randomized Design

AbstractRice is an important staple crop produced and consumed worldwide. However, poor seed emergence is one of the main impediments to obtaining higher yield of rice especially in hot and dry ecosystems of the world that are ravaged by drought. Therefore, this study was carried out to evaluate the effects of potassium nitrate (KNO3), salicylic acid (SA) and silicon dioxide (SiO2) priming in improving emergence, seedling growth, biochemical attributes and antioxidant activities of FARO44 rice under drought conditions. Rice seedlings primed with 2.5% and 5% KNO3, 3% and 3.5% SiO2, and 1 mM and 2.5 mM SA were subjected to three drought levels of low, moderate and severe under the greenhouse. Seed emergence, seedling growth, biochemical attributes and antioxidant activities were thereafter evaluated. Seed priming experiments were laid in a completely randomized design with five replicates per treatment. The results found that rice seedlings responded differently to different priming treatments. However, all primed rice seedlings had significantly (P ≤ 0.05) improved emergence percentage (72–92%), seedling growth, seedling vigor, seedling fresh and dry biomass and shorter emergence time compared with controls. Likewise, total soluble protein content, activities of catalase, ascorbate peroxidase and superoxide dismutase, carbohydrate, soluble sugar and total chlorophyll contents of rice seedlings were increased by more than two-folds by seed priming compared with control. Salicylic acid showed less effect in increasing emergence, seedling growth, antioxidant activities and biochemical attributes of rice. Thus, this study established that seed priming with KNO3 (2.5% and 5%) and SiO2 (3% and 3.5%) were more effective in improving emergence, seedling growth, biochemical attributes and antioxidant activities of FARO44. Thus, priming of FARO44 rice with this chemical is recommended for fast emergence, seedling growth and drought resistance in dry ecosystems.

Mapping of quantitative trait loci for gibberellic acid response at rice (Oryza sativa L.) seedling stage

Plant Science ◽  
2006 ◽  
Vol 170 (1) ◽  
pp. 12-17 ◽  
Author(s):  
Yanjun Dong ◽  
H. Kamiuten ◽  
Zhongnan Yang ◽  
Dongzhi Lin ◽  
T. Ogawa ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Quantitative Trait Loci ◽  
Gibberellic Acid ◽  
Quantitative Trait ◽  
Oryza Sativa L ◽  
Seedling Stage ◽  
Trait Loci
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