scholarly journals Sodium Chloride Stress Induced Changes in Leaf Osmotic Adjustment of Trifoliate Orange (Poncirus trifoliata) Seedlings Inoculated with Mycorrhizal Fungi

2011 ◽  
Vol 39 (2) ◽  
pp. 64 ◽  
Author(s):  
Ying-Ning ZOU ◽  
Qiang-Sheng WU
Keyword(s):  
Osmotic Adjustment ◽  
Mycorrhizal Fungi ◽  
Plant Biomass ◽  
Arbuscular Mycorrhizal ◽  
Arbuscular Mycorrhizas ◽  
Mycorrhizal Colonization ◽  
Poncirus Trifoliata ◽  
Mycorrhizal Symbiosis ◽  
Trifoliate Orange ◽  
K Concentration

Citrus plants are sensitive to salinity, and thus employing new approaches to alleviate salt damage are necessary. The present study evaluated the effect of two arbuscular mycorrhizal fungi (AMF), Glomus mosseae and G. versiforme, on leaf osmotic adjustment of trifoliate orange (Poncirus trifoliata) seedings exposed to 100 mM NaCl. Salinity significantly inhibited mycorrhizal colonization, plant biomass and leaf relative water content, whereas the reduce of plant biomass was notably alleviated by the mycorrhizal colonization. Mycorrhizal seedlings exhibited significantly lower Na+ and Ca2+ concentrations, whilst also recorded higher K+ concentration and K+/Na+, Ca2+/Na+ and Mg2+/Na+ ratios at both salinity levels. Under salinity stress, mycorrhizal symbiosis markedly decreased sucrose concentrations of leaves and also increased glucose, fructose and proline concentrations of leaves. The results suggest that arbuscular mycorrhizas improved leaf osmotic adjustment responses of the seedlings to salt stress, thus enhancing salt tolerance of mycorrhizal plants.

scholarly journals Exogenous Carbon Magnifies Mycorrhizal Effects on Growth Behaviour and Sucrose Metabolism in Trifoliate Orange

2018 ◽  
Vol 46 (2) ◽  
pp. 365-370 ◽  
Author(s):  
Li TIAN ◽  
Yan LI ◽  
Qiang-Sheng WU
Keyword(s):  
Plant Growth ◽  
Root Morphology ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Arbuscular Mycorrhizas ◽  
Sucrose Metabolism ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Positive Effects ◽  
Mycorrhizal Plants

Arbuscular mycorrhizas (AMs) need the carbohydrates from host plants for its growth, whereas it is not clear whether exogenous carbon affects mycorrhizal roles. A two-chambered rootbox was divided into root + hyphae chamber and hyphae chamber (free of roots) by 37-μm nylon mesh, in which trifoliate orange (Poncirus trifoliata) seedlings and Funneliformis mosseae were applied into root + hyphae chamber, and exogenous 40 mmol/L fructose, glucose and sucrose was applied to hyphae chamber. Application of exogenous sugars dramatically elevated root mycorrhizal colonization. Sole arbuscular mycorrhizal fungi (AMF) inoculation significantly promoted plant growth and root morphology than non-AMF treatment. Mycorrhiza-improved plant growth and root modification could be enlarged by exogenous carbon, especially fructose. Exogenous carbon markedly increased root fructose, glucose and sucrose accumulation in mycorrhizal plants, especially sucrose. Exogenous fructose significantly reduced leaf and root sucrose synthase (SS) activity in synthesis direction and increased them in cleavage direction in AMF seedlings. Exogenous glucose and sucrose heavily elevated root SS activity of mycorrhizal seedlings in synthesis and cleavage direction and reduced leaf SS activity in synthesis direction. Leaf acid invertase (AI) and neutral invertase (NI) activities of mycorrhizal seedlings were decreased by exogenous carbon, except sucrose in NI. Exogenous fructose significantly increased root AI and NI activity in mycorrhizal plants. These results implied that mycorrhizal inoculation represented positive effects on plant growth, root morphology, and sucrose metabolism of trifoliate orange, which could be magnified further by exogenous carbon, especially fructose.

scholarly journals Exogenous Glomalin-Related Soil Proteins Differentially Regulate Soil Properties in Trifoliate Orange

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1896
Author(s):  
Rui-Cheng Liu ◽  
Ying-Ning Zou ◽  
Kamil Kuča ◽  
Abeer Hashem ◽  
Elsayed Fathi Abd_Allah ◽  
...  
Keyword(s):  
Soil Properties ◽  
Phosphatase Activity ◽  
Mycorrhizal Fungi ◽  
Root Biomass ◽  
Plant Biomass ◽  
Arbuscular Mycorrhizal ◽  
Poncirus Trifoliata ◽  
Organic Carbon Content ◽  
Trifoliate Orange ◽  
Shoot And Root Biomass

Glomalin-related soil protein (GRSP) is a specific glycoprotein secreted into the soil by hyphae and spores of arbuscular mycorrhizal fungi that have many potential functions. It is not clear whether exogenous GRSP has an effect on plant growth and soil properties or whether the effects are related to the type of GRSP used. In this study, trifoliate orange (Poncirus trifoliata L. Raf.) seedlings were used to analyze the effects of easily extractable GRSP (EE-GRSP) and difficultly extractable GRSP (DE-GRSP) at a quarter-, half-, and full-strength concentration on shoot and root biomass as well as soil properties The results showed that, at different strengths, exogenous EE-GRSR significantly increased shoot and root biomass compared to the control, which displayed the most significant effects from the half-strength EE-GRSP. In contrast, DE-GRSP, at various strengths, significantly reduced shoot and root biomass. Furthermore, the application of exogenous EE-GRSP stimulated soil water-stable aggregate (WSA) content at 2–4 mm and 0.5–1 mm sizes, while DE-GRSP strongly reduced WSA content at the 2–4 mm, 1–2 mm, 0.5–1 mm, and 0.25–0.5 mm sizes, consequently leading to an increase or decrease in the WSA stability, according to the mean weight diameter. However, exogenous EE-GRSP decreased soil pH and DE-GRSP increased it, which was related to WSA stability. Exogenous EE-GRSP almost significantly increased soil acidic, neutral, and alkaline phosphatase activity at different strengths, while exogenous DE-GRSP, also at different strengths, significantly inhibited soil acidic phosphatase activity. The application of both exogenous EE-GRSP and DE-GRSP increased the organic carbon content of the soil. This study concluded that exogenous GRSP exerted differential effects on plant biomass and soil properties, and EE-GRSP can be considered as a soil stimulant for use in citrus plants. To our knowledge, this is the first report on the negative effects of exogenous DE-GRSP on plant biomass and soil properties.

scholarly journals Community Analysis of Arbuscular Mycorrhizal Fungi in Roots ofPoncirus trifoliataandCitrus reticulataBased on SSU rDNA

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Wang ◽  
Yin Wang
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Small Subunit ◽  
Poncirus Trifoliata ◽  
Spore Density ◽  
Morphological Observation ◽  
Trifoliate Orange ◽  
Hyphal Length

Morphological observation of arbuscular mycorrhizal fungi (AMF) species in rhizospheric soil could not accurately reflect the actual AMF colonizing status in roots, while molecular identification of indigenous AMF colonizing citrus rootstocks at present was rare in China. In our study, community of AMF colonizing trifoliate orange (Poncirus trifoliataL. Raf.) and red tangerine (Citrus reticulataBlanco) were analyzed based on small subunit of ribosomal DNA genes. Morphological observation showed that arbuscular mycorrhizal (AM) colonization, spore density, and hyphal length did not differ significantly between two rootstocks. Phylogenetic analysis showed that 173 screened AMF sequences clustered in at least 10 discrete groups (GLO1~GLO10), all belonging to the genus ofGlomusSensu Lato. Among them, GLO1 clade (clustering with uncultured Glomus) accounting for 54.43% clones was the most common in trifoliate orange roots, while GLO6 clade (clustering withGlomus intraradices) accounting for 35.00% clones was the most common in red tangerine roots. Although, Shannon-Wiener indices exhibited no notable differences between both rootstocks, relative proportions of observed clades analysis revealed that composition of AMF communities colonizing two rootstocks varied severely. The results indicated that native AMF species in citrus rhizosphere had diverse colonization potential between two different rootstocks in the present orchards.

scholarly journals Enhancement of Drought Tolerance in Trifoliate Orange by Mycorrhiza: Changes in Root Sucrose and Proline Metabolisms

2018 ◽  
Vol 46 (1) ◽  
pp. 270-276 ◽  
Author(s):  
Fei ZHANG ◽  
Jia-Dong HE ◽  
Qiu-Dan NI ◽  
Qiang-Sheng WU ◽  
Ying-Ning ZOU
Keyword(s):  
Drought Tolerance ◽  
Mycorrhizal Fungi ◽  
Water Status ◽  
Lateral Roots ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Sucrose Phosphate Synthase ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Am Symbiosis

Sucrose and proline metabolisms are often associated with drought tolerance of plants. This study was conducted to investigate the effects of two arbuscular mycorrhizal fungi (AMF) species (Funneliformis mosseae and Paraglomus occultum) on root biomass, lateral root number, root sucrose and proline metabolisms in trifoliate orange (Poncirus trifoliata) seedlings under well-watered (WW) or drought stress (DS). All the AMF treatments significantly increased root dry weight, taproot length, and the number of lateral roots in 1st, 2nd, and 3rd class under WW and DS. Mycorrhizal seedlings conferred considerably higher fructose and glucose concentrations but lower sucrose accumulation, regardless of soil water status. Under DS, F. mosseae treatment significantly increased root sucrose synthase (SS, degradative direction) and sucrose phosphate synthase (SPS) activity but deceased root acid invertase (AI) and neutral invertase (NI) activity, and P. occultum inoculation markedly increased root AI, NI, SS, and SPS activities. AMF treatments led to a lower proline accumulation in roots, in company with lower activities of Δ1-pyrroline-5-carboxylate synthetase (P5CS), δ-ornithine aminotransferase (OAT), Δ1-pyrroline-5-carboxylate reductase (P5CR), and proline dehydrogenase (ProDH) in roots. It appears that the AM symbiosis induced greater root development and sucrose and proline metabolisms to adapt DS.

The influence of mycorrhizal colonization on the vegetative growth and sexual reproductive potential of Lythrum salicaria L.

2001 ◽  
Vol 79 (4) ◽  
pp. 381-388 ◽  
Author(s):  
Leanne J Philip ◽  
Usher Posluszny ◽  
John N Klironomos
Keyword(s):  
Sexual Reproduction ◽  
Vegetative Growth ◽  
Mycorrhizal Fungi ◽  
Plant Biomass ◽  
Mycorrhizal Fungus ◽  
Reproductive Potential ◽  
Arbuscular Mycorrhizal ◽  
Lythrum Salicaria ◽  
Mycorrhizal Colonization ◽  
Purple Loosestrife

Lythrum salicaria L., purple loosestrife, is a heterostylous, perennial plant with prolific and at times invasive vegetative growth and sexual reproduction. Sexual reproduction occurs following pollination and fertilization between two different floral morphs. We investigated the influence of the arbuscular mycorrhizal fungus, Glomus aggregatum Schenck and Smith emend. Koske, on the vegetative growth and sexual reproductive potential of L. salicaria. Mycorrhiza decreased plant biomass both aboveground and belowground. Flower production, number of days to anthesis, numbers of flowers per inflorescence, and inflorescence lengths were not significantly different between mycorrhizal and non-mycorrhizal treatments. However, pollen production per anther and per flower increased with mycorrhizal colonization. Though ovule production was not affected, some aspects of purple loosestrife morphology did change. Plants with mycorrhizae produced inflorescence (in lateral positions) further up the stem. In addition, flower distribution within an inflorescence differed according to morph (short, mid, and long style) such that in the mid and long morphs flower number increased with inflorescence length and was unevenly distributed, while in the short morph this distribution appeared even. The relative biomass of stems, leaves, lateral branches, and reproductive structures were not significantly different in mycorrhizal plants, whereas in the absence of mycorrhizal colonization, stem biomass was higher relative to other structures. This study suggests some vegetative and reproductive characteristics in purple loosestrife change with an association with arbuscular mycorrhizal fungi.Key words: purple loosestrife, arbuscular mycorrhizal fungi, plant reproduction.

scholarly journals Metabolomics Analysis Reveals Drought Responses of Trifoliate Orange by Arbuscular Mycorrhizal Fungi With a Focus on Terpenoid Profile

2021 ◽  
Vol 12 ◽  
Author(s):  
Sheng-Min Liang ◽  
Fei Zhang ◽  
Ying-Ning Zou ◽  
Kamil Kuča ◽  
Qiang-Sheng Wu
Keyword(s):  
Drought Stress ◽  
Arbuscular Mycorrhizal Fungi ◽  
Soil Water ◽  
Mycorrhizal Fungi ◽  
Metabolic Pathways ◽  
Arbuscular Mycorrhizal ◽  
Poncirus Trifoliata ◽  
Soil Drought ◽  
Trifoliate Orange ◽  
Normal Water

Soil water deficit seriously affects crop production, and soil arbuscular mycorrhizal fungi (AMF) enhance drought tolerance in crops by unclear mechanisms. Our study aimed to analyze changes in non-targeted metabolomics in roots of trifoliate orange (Poncirus trifoliata) seedlings under well-watered and soil drought after inoculation with Rhizophagus intraradices, with a focus on terpenoid profile. Root mycorrhizal fungal colonization varied from 70% under soil drought to 85% under soil well-watered, and shoot and root biomass was increased by AMF inoculation, independent of soil water regimes. A total of 643 secondary metabolites in roots were examined, and 210 and 105 differential metabolites were regulated by mycorrhizal fungi under normal water and drought stress, along with 88 and 17 metabolites being up-and down-regulated under drought conditions, respectively. KEGG annotation analysis of differential metabolites showed 38 and 36 metabolic pathways by mycorrhizal inoculation under normal water and drought stress conditions, respectively. Among them, 33 metabolic pathways for mycorrhization under drought stress included purine metabolism, pyrimidine metabolism, alanine, aspartate and glutamate metabolism, etc. We also identified 10 terpenoid substances, namely albiflorin, artemisinin (−)-camphor, capsanthin, β-caryophyllene, limonin, phytol, roseoside, sweroside, and α-terpineol. AMF colonization triggered the decline of almost all differential terpenoids, except for β-caryophyllene, which was up-regulated by mycorrhizas under drought, suggesting potential increase in volatile organic compounds to initiate plant defense responses. This study provided an overview of AMF-induced metabolites and metabolic pathways in plants under drought, focusing on the terpenoid profile.

scholarly journals Effect of Vermicompost and Selected Plant Preparations on the Development of Arbuscular Mycorrhizal Symbiosis in Lettuce (Lactuca sativa)

2018 ◽  
Vol 66 (1) ◽  
pp. 195-201
Author(s):  
Michaela Stroblová ◽  
Ladislava Prokopová ◽  
Jaroslav Záhora
Keyword(s):  
Mycorrhizal Fungi ◽  
Dry Matter ◽  
Plant Biomass ◽  
Arbuscular Mycorrhizal ◽  
Arbuscular Mycorrhizal Symbiosis ◽  
Mycorrhizal Symbiosis ◽  
Extraradical Mycelium ◽  
Significant Difference ◽  
Plant Biomass Production ◽  
Dry Matter Weight

The aim of the pot experiment was to evaluate effect of vermicompost and promoting plant preparations application (Symbivit and Plantaktiv) on the development of arbuscular mycorrhizal symbiosis in lettuce, amount of extraradical mycelium of mycorrhizal fungi in soil and plant biomass production. Symbivit contains six species of mycorrhizal fungi and Plantaktiv contains magnesium sulfate activated by oxygen promoting activity of aerobic microorganisms in the soil. The application of vermicompost and promoting plant preparations did not have a statistically significant effect on lettuce root colonization by mycorrhizal fungi. The greatest length of extraradical mycelium was observed in variant with vermicompost application, in variant with addition of vermicompost and Symbivit, and in variant with vermicompost applied with both plant preparations. There was detected statistically significant difference when compared to control variant. Dry matter weight of aboveground biomass and root of the lettuce were statistically significantly increased in all variants, where vermicompost was applied, either alone or in combination with plant preparations. Separate application of Symbivit or Plantaktiv did not have a statistically significant effect either on the length of extraradical mycelium or dry matter weight of the lettuce.

scholarly journals Arbuscular Mycorrhizal Symbiosis Mitigates Iron (Fe)-Deficiency Retardation in Alfalfa (Medicago sativa L.) Through the Enhancement of Fe Accumulation and Sulfur-Assisted Antioxidant Defense

2020 ◽  
Vol 21 (6) ◽  
pp. 2219 ◽  
Author(s):  
Md. Atikur Rahman ◽  
Monika Parvin ◽  
Urmi Das ◽  
Esrat Jahan Ela ◽  
Sang-Hoon Lee ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Mycorrhizal Fungi ◽  
Antioxidant Defense ◽  
Plant Biomass ◽  
Reductase Activity ◽  
Arbuscular Mycorrhizal ◽  
Fe Deficiency ◽  
Mycorrhizal Symbiosis ◽  
Uptake System ◽  
Medicago Sativa L

Iron (Fe)-deficiency is one of the major constraints affecting growth, yield and nutritional quality in plants. This study was performed to elucidate how arbuscular mycorrhizal fungi (AMF) alleviate Fe-deficiency retardation in alfalfa (Medicago sativa L.). AMF supplementation improved plant biomass, chlorophyll score, Fv/Fm (quantum efficiency of photosystem II), and Pi_ABS (photosynthesis performance index), and reduced cell death, electrolyte leakage, and hydrogen peroxide accumulation in alfalfa. Moreover, AMF enhanced ferric chelate reductase activity as well as Fe, Zn, S and P in alfalfa under Fe-deficiency. Although Fe-transporters (MsIRT1 and MsNramp1) did not induce in root but MsFRO1 significantly induced by AMF under Fe deficiency in roots, suggesting that AMF-mediated Fe enhancement is related to the bioavailability of Fe at rhizosphere/root apoplast rather than the upregulation of Fe transporters under Fe deficiency in alfalfa. Several S-transporters (MsSULTR1;1, MsSULTR1;2, MsSULTR1;3, and MsSULTR3;1) markedly increased following AMF supplementation with or without Fe-deficiency alfalfa. Our study further suggests that Fe uptake system is independently influenced by AMF regardless of the S status in alfalfa. However, the increase of S in alfalfa is correlated with the elevation of GR and S-metabolites (glutathione and cysteine) associated with antioxidant defense under Fe deficiency.

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