Management of Bacterial Kidney Disease in Chinook Salmon Hatcheries Based on Broodstock Testing by Enzyme-Linked Immunosorbent Assay: A Multiyear Study

2010 ◽  
Vol 30 (4) ◽  
pp. 940-955 ◽  
Author(s):  
A. Douglas Munson ◽  
Diane G. Elliott ◽  
Keith Johnson
Keyword(s):  
Kidney Disease ◽  
Immunosorbent Assay ◽  
Chinook Salmon ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Kidney Disease

Genetic effects of ELISA-based segregation for control of bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha)

2006 ◽  
Vol 63 (12) ◽  
pp. 2793-2808 ◽  
Author(s):  
Jeffrey J Hard ◽  
Diane G Elliott ◽  
Ronald J Pascho ◽  
Dorothy M Chase ◽  
Linda K Park ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Level ◽  
Renibacterium Salmoninarum ◽  
Listonella Anguillarum ◽  
Bacterial Kidney Disease ◽  
Genetic And Phenotypic Correlations ◽  
High Heritability

We evaluated genetic variation in ability of Chinook salmon (Oncorhynchus tshawytscha) to resist two bacterial pathogens: Renibacterium salmoninarum, the agent of bacterial kidney disease (BKD), and Listonella anguillarum, an agent of vibriosis. After measuring R. salmoninarum antigen in 499 adults by enzyme-linked immunosorbent assay (ELISA), we mated each of 12 males with high or low antigen levels to two females with low to moderate levels and exposed subsets of their progeny to each pathogen separately. We found no correlation between R. salmoninarum antigen level in parents and survival of their progeny following pathogen exposure. We estimated high heritability for resistance to R. salmoninarum (survival h2 = 0.890 ± 0.256 (mean ± standard error)) independent of parental antigen level, but low heritability for resistance to L. anguillarum (h2 = 0.128 ± 0.078). The genetic correlation between these survivals (rA = –0.204 ± 0.309) was near zero. The genetic and phenotypic correlations between survival and antigen levels among surviving progeny exposed to R. salmoninarum were both negative (rA = –0.716 ± 0.140; rP = –0.378 ± 0.041), indicating that variation in antigen level is linked to survival. These results suggest that selective culling of female broodstock with high antigen titers, which is effective in controlling BKD in salmon hatcheries, will not affect resistance of their progeny.

scholarly journals DIFFERENCES OF SALIVA LEPTIN LEVELS IN CHILDREN WITH CHRONIC KIDNEY DISEASE ON HEMODIALYSIS AND HEALTHY CHILDREN (STUDY IN CHILDREN WITH GINGIVITIS)

2018 ◽  
Vol 11 (4) ◽  
pp. 192
Author(s):  
Berthauli Esther Nurmaida ◽  
Heriandi Sutadi ◽  
Sarworini B Budiardjo ◽  
Eka Laksmi Hidayati
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Children ◽  
Significant Difference ◽  
The Difference

 Objectives: Analyze the difference of salivary leptin in between healthy children with gingivitis and hemodialysis (HD) children with gingivitis.Methods: A total of 20 children, ages 11–16-year-old with gingivitis, were chosen as subjects; 10 were on HD and 10 were healthy children. The level of salivary leptin was measured using the enzyme-linked immunosorbent assay method.Results: The results showed a significant difference of salivary leptin levels between the children on HD (61,300 ± 4151 pg/ml) and the healthy children (57,200 ± 3173 pg/ml).Conclusions: There is a significant difference in the salivary leptin levels in children on HD with gingivitis and healthy children with gingivitis.

scholarly journals Both msa Genes in Renibacterium salmoninarum Are Needed for Full Virulence in Bacterial Kidney Disease

2006 ◽  
Vol 72 (4) ◽  
pp. 2672-2678 ◽  
Author(s):  
Alison M. Coady ◽  
Anthony L. Murray ◽  
Diane G. Elliott ◽  
Linda D. Rhodes
Keyword(s):  
Kidney Disease ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Soluble Antigen ◽  
Renibacterium Salmoninarum ◽  
Juvenile Chinook Salmon ◽  
Bacterial Kidney Disease ◽  
Important Virulence Factor ◽  
Chromosomal Loci

ABSTRACT Renibacterium salmoninarum, a gram-positive diplococcobacillus that causes bacterial kidney disease among salmon and trout, has two chromosomal loci encoding the major soluble antigen (msa) gene. Because the MSA protein is widely suspected to be an important virulence factor, we used insertion-duplication mutagenesis to generate disruptions of either the msa1 or msa2 gene. Surprisingly, expression of MSA protein in broth cultures appeared unaffected. However, the virulence of either mutant in juvenile chinook salmon (Oncorhynchus tshawytscha) by intraperitoneal challenge was severely attenuated, suggesting that disruption of the msa1 or msa2 gene affected in vivo expression.

Enzyme-linked Immunosorbent Assay for a Soluble Antigen of Renibacterium salmoninarum, the Causative Agent of Salmonid Bacterial Kidney Disease

1987 ◽  
Vol 44 (1) ◽  
pp. 183-191 ◽  
Author(s):  
R. J. Pascho ◽  
D. Mulcahy
Keyword(s):  
Immunosorbent Assay ◽  
Incubation Temperature ◽  
Tween 80 ◽  
Broth Culture ◽  
Tween 20 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Soluble Antigen ◽  
Renibacterium Salmoninarum ◽  
Bacterial Kidney Disease ◽  
Wash Buffer

A double-antibody enzyme-linked immunosorbent assay (ELISA) for detection of a soluble fraction of Renibacterium salmoninarum was developed from components extracted from the supernatant of an R. salmoninarum broth culture. The Costar® Serocluster™ EIA microplate gave the highest absorbance and signal-to-noise ratios among seven types tested. Including Tween 80 in the wash buffer resulted in higher absorbances than Tween 20 when antigen was present. Background absorbance did not increase when Tween 80 was added to the wash buffer, but did when Tween 80 replaced Tween 20 in antigen and conjugate diluents. Adsorption of coating antibody peaked within 4 h at 37 °C and 16 h at 4 °C. Antigen attachment to antibody-coated microplate wells depended more on incubation temperature than duration; we adopted a 3-h incubation at 25 °C. Conjugate incubation for longer than 1 h at 37 °C or 3 h at 25 °C resulted in unacceptable background levels. No cross-reactions resulted from heat-extracted antigens of 10 other species of bacteria. The optimized ELISA is a 6-h test that enables detection of levels of soluble antigen as low as 2–20 ng.

scholarly journals Experimental induction of bacterial kidney disease in Chinook salmon by immersion and cohabitation challenges

1992 ◽  
Vol 12 ◽  
pp. 91-96 ◽  
Author(s):  
CB Murray ◽  
TPT Evelyn ◽  
TD Beacham ◽  
LW Bamer ◽  
JE Ketcheson ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Chinook Salmon ◽  
Bacterial Kidney Disease ◽  
Experimental Induction
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