Cellular protein thiols: studies on human prostate cell lines; a lymph node cancer line (LNCaP) and a virally transformed normal cell line (PNT2)

Abstract Background Breast cancer (BC), as the most widely recognized disease in women worldwide, represents about 30% of all cancers impacting women. This study was aimed to synthesize Cu2O nanoparticles from the cystoseira myrica algae (CM-Cu2O NPs) assess their antimicrobial activity against pathogenic bacteria and fungi. We evaluated the expression levels of lncRNAs (MALAT1 and GAS5) and apoptosis genes (p53, p27, bax, bcl2 and caspase3), their prognostic roles. Methods In this study, CM-Cu2O NPs synthesized by cystoseira myrica algae extraction used to evaluate its cytotoxicity and apoptotic properties on MDA-MB-231, SKBR3 and T-47D BC cell lines compared to HDF control cell line. The CM-Cu2O NPs was characterized by UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM). The antimicrobial activity of CM-Cu2O NPs was assessed against pathogenic bacteria, staphylococcus aureus (S. aureus) PTCC 1112 bacteria as a standard gram-positive bacteria and pseudomonas aeruginosa (P. aeruginosa) PTCC 1310 as a standard gram-negative bacterium. Expression profile of MALAT1 and GAS5 lncRNAs and apoptosis genes, i.e., p27, bax, bcl2 and caspase3 genes, were calculated utilizing qRT-PCR. The changes in the expression levels were determined using the DDCT method. Results MALAT1 was upregulated in MDA-MB-231, SKBR3 and T-47D BC (p < 0.01), while GAS5 was downregulated in SKBR3 and T-47D cell lines tested compared with HDF control cell line (p < 0.05) was found. The results revealed that, p27, bax and caspase3 were significantly upregulated in BC cell lines as compared with normal cell line. Bcl2 expression was also significantly increased in MDA-MB-231 and T47D cell lines compared with normal cell line, but bcl2 levels were downregulated in SKBR3 cell line. Conclusions Our results confirm the beneficial cytotoxic effects of green-synthesized CM-Cu2O NPs on BC cell lines. This nanoparticle decreased angiogenesis and induces apoptosis, so we conclude that CM-Cu2O NPs can be used as a supplemental drug in cancer treatments. Significantly, elevated circulating lncRNAs were demonstrated to be BC specific and could differentiate BC cell lines from the normal cell lines. It was demonstrated that lncRNAs used in this study and their expression profiles can be created as biomarkers for early diagnosis and prognosis of BC. Further studies utilizing patients would give recognizable identification of lncRNAs as key players in intercellular interactions.

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Coexpression of CAV-1, AT1-R and FOXM1 in prostate and breast cancer and normal cell lines and their influence on metastatic properties

Acta Biochimica Polonica ◽

10.18388/abp.2015_1016 ◽

2016 ◽

Vol 63 (3) ◽

Cited By ~ 7

Author(s):

Karolina Kowalska ◽

Magdalena Nowakowska ◽

Kamila Domińska ◽

Agnieszka W. Piastowska-Ciesielska

Keyword(s):

Breast Cancer ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Normal Cell ◽

Metastatic Potential ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Prostate Cell

The aim of this study was to evaluate the coexpression of caveolin-1 (CAV-1), angiotensin II type 1 receptor (AT1-R) and forkhead box Ml (FOXM1) in prostate and breast cancer cell lines, in comparison with normal cell lines. CAV-1, AT1-R and FOXM1 expression was determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis in the prostate cancer cell lines PC3, DU145 and LNCaP; prostate normal cell line PNT1A; breast cancer cell lines MCF-7 and MDA-MB-231; and the normal breast cell line 184A1. A correlation between the expression levels of the investigated genes and their metastatic properties was determined by the Spearman's rank test (P<0.05) and Aspin-Welsch t-test, respectively. In prostate cell lines, a significant correlation was noted between CAV-1 and AT1-R expression and between FOXM1 and CAV-1 expression. A correlation between the expression levels of the investigated genes and their metastatic potential was also observed, with relatively high expression of all the investigated genes in the normal prostate cell line PNT1A. In comparison to prostate cancer cell lines, an adverse dependency between CAV-1, AT1-R, FOXM1 expression and metastatic potential was observed in the breast cancer cell lines. Relatively high expression of all tested genes was observed in the normal breast cell line 184A1, which was decreasing respectively with increasing metastatic potential of breast cancer cell lines. The results obtained here indicate that CAV-1, FOXM1 and AT1-R may be potential markers of tumorigenesis in certain types of cancer in vitro.

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Gene expression analysis of human prostate cell lines with and without tumor metastasis suppressor CD82

BMC Cancer ◽

10.1186/s12885-020-07675-7 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Pushpaja Dodla ◽

Vanitha Bhoopalan ◽

Sok Kean Khoo ◽

Cindy Miranti ◽

Suganthi Sridhar

Keyword(s):

Gene Expression ◽

Cell Line ◽

Cell Lines ◽

Differentially Expressed Genes ◽

Tumor Metastasis ◽

Human Prostate ◽

Metastasis Suppressor ◽

Data Sets ◽

Prostate Cell ◽

Prostate Cell Lines

Abstract Background Tetraspanin CD82 is a tumor metastasis suppressor that is known to down regulate in various metastatic cancers. However, the exact mechanism by which CD82 prevents cancer metastasis is unclear. This study aims to identify genes that are regulated by CD82 in human prostate cell lines. Methods We used whole human genome microarray to obtain gene expression profiles in a normal prostate epithelial cell line that expressed CD82 (PrEC-31) and a metastatic prostate cell line that does not express CD82 (PC3). Then, siRNA silencing was used to knock down CD82 expression in PrEC-31 while CD82 was re-expressed in PC3 to acquire differentially-expressed genes in the respective cell line. Results Differentially-expressed genes with a P < 0.05 were identified in 3 data sets: PrEC-31 (+CD82) vs PrEC-31(−CD82), PC3–57 (+CD82) vs. PC3-5 V (−CD82), and PC3–29 (+CD82) vs. PC3-5 V (−CD82). Top 25 gene lists did not show overlap within the data sets, except (CALB1) the calcium binding protein calbindin 1 which was significantly up-regulated (2.8 log fold change) in PrEC-31 and PC3–29 cells that expressed CD82. Other most significantly up-regulated genes included serine peptidase inhibitor kazal type 1 (SPINK1) and polypeptide N-acetyl galactosaminyl transferase 14 (GALNT14) and most down-regulated genes included C-X-C motif chemokine ligand 14 (CXCL14), urotensin 2 (UTS2D), and fibroblast growth factor 13 (FGF13). Pathways related with cell proliferation and angiogenesis, migration and invasion, cell death, cell cycle, signal transduction, and metabolism were highly enriched in cells that lack CD82 expression. Expression of two mutually inclusive genes in top 100 gene lists of all data sets, runt-related transcription factor (RUNX3) and trefoil factor 3 (TFF3), could be validated with qRT-PCR. Conclusion Identification of genes and pathways regulated by CD82 in this study may provide additional insights into the role that CD82 plays in prostate tumor progression and metastasis, as well as identify potential targets for therapeutic intervention.

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miR-27a-3p Functions as a Tumor Suppressor and Regulates Non-Small Cell Lung Cancer Cell Proliferation via Targeting HOXB8

Technology in Cancer Research & Treatment ◽

10.1177/1533033819861971 ◽

2019 ◽

Vol 18 ◽

pp. 153303381986197 ◽

Cited By ~ 5

Author(s):

Xiaohong Yan ◽

Hui Yu ◽

Yao Liu ◽

Jie Hou ◽

Qiao Yang ◽

...

Keyword(s):

Lung Cancer ◽

Cell Proliferation ◽

Tumor Suppressor ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Normal Cell ◽

Cancer Cell Proliferation ◽

Lung Cancer Cell ◽

Normal Cell Line

MicroRNA-27a-3p has been implicated to play crucial roles in human cancers. However, the biological role and underlying mechanisms of microRNA-27a-3p in regulating nonsmall lung cancer remain unclear. MicroRNA-27a-3p expression levels in non-small lung cancer cell lines were detected by quantitative real-time polymerase chain reaction, using a normal cell line as control. The effects of microRNA-27a-3p on cell proliferation and apoptosis were analyzed by Cell Counting Kit-8 assay and flow cytometry assay. Luciferase activity reporter assay and Western blot were conducted to validate the potential targets of miR27a-3p after preliminary screening by TargetScan. Effect of microRNA-27a-3p or homeobox B8 on the overall survival of patients with non-small lung cancer was analyzed at Kaplan-Meier Plotter website. MicroRNA-27a-3p expression levels were significantly reduced in non-small lung cancer cell lines compared with normal cell line. Overexpression of microRNA-27a-3p inhibits non-small lung cancer cell proliferation but promotes cell apoptosis. Homeobox B8 was further validated as a functional target of microRNA-27a-3p. Collectively, our results indicated that microRNA-27a-3p acts as a tumor suppressor in non-small lung cancer via targeting homeobox B8.

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In vitro anticancer activity of methanolic extract of Granulocystopsis sp., a microalgae from an oligotrophic oasis in the Chihuahuan desert

PeerJ ◽

10.7717/peerj.8686 ◽

2020 ◽

Vol 8 ◽

pp. e8686

Author(s):

Faviola Tavares-Carreón ◽

Susana De la Torre-Zavala ◽

Hector Fernando Arocha-Garza ◽

Valeria Souza ◽

Luis J. Galán-Wong ◽

...

Keyword(s):

Anticancer Activity ◽

Cell Line ◽

Cell Lines ◽

Normal Cell ◽

Chihuahuan Desert ◽

Methanolic Extract ◽

Membrane Integrity ◽

Skin Melanoma ◽

Normal Cell Line

With the purpose of discovering new anticancer molecules that might have fewer side effects or reduce resistance to current antitumor drugs, a bioprospecting study of the microalgae of the Cuatro Cienegas Basin (CCB), an oasis in the Chihuahuan desert in Mexico was conducted. A microalgae was identified as Granulocystopsis sp. through sequencing the rbcL gene and reconstruction of a phylogenetic tree, and its anticancer activities were assessed using various in vitro assays and different cell lines of human cancers, including lung, skin melanoma, colorectal, breast and prostatic cancers, as well as a normal cell line. The values of IC50 of the microalgae methanolic extract using the MTT assay were lower than 20 μg/ml, except that in the lung cancer line and the normal cell line. In vitro, the microalgae extract caused the loss of membrane integrity, monitored by the trypan blue exclusion test and exhibited marked inhibition of adhesion and cell proliferation in cancer cell lines, through the evaluation of the clonogenic assay. Also, typical nuclear changes of apoptotic processes were observed under the microscope, using the dual acridine orange/ethidium bromide fluorescent staining. Finally, the microalgae extract increased the activity of caspases 3 and 7 in skin melanoma, colon, breast and prostate cancer cells, in the same way as the apoptotic inductor and powerful antitumoral drug, doxorubicin. This study shows the anticancer activity from Granulocystopsis sp., a microalgae isolated from the CCB.

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Stable W/O/W multiple nanoemulsion encapsulating natural tocotrienols and caffeic acid with cisplatin synergistically treated cancer cell lines (A549 and HEP G2) and reduced toxicity on normal cell line (HEK 293)

Materials Science and Engineering C ◽

10.1016/j.msec.2020.111808 ◽

2021 ◽

Vol 121 ◽

pp. 111808

Author(s):

Revathi Raviadaran ◽

Mei Han Ng ◽

Davannendran Chandran ◽

Kah Kooi Ooi ◽

Sivakumar Manickam

Keyword(s):

Cell Line ◽

Caffeic Acid ◽

Cell Lines ◽

Cancer Cell ◽

Normal Cell ◽

Cancer Cell Lines ◽

Hep G2 ◽

Hek 293 ◽

Normal Cell Line ◽

Reduced Toxicity

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Difference in Membrane Repair Capacity Between Cancer Cell Lines and a Normal Cell Line

The Journal of Membrane Biology ◽

10.1007/s00232-016-9910-5 ◽

2016 ◽

Vol 249 (4) ◽

pp. 569-576 ◽

Cited By ~ 18

Author(s):

Stine Krog Frandsen ◽

Anna K. McNeil ◽

Ivana Novak ◽

Paul L. McNeil ◽

Julie Gehl

Keyword(s):

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Normal Cell ◽

Cancer Cell Lines ◽

Membrane Repair ◽

Repair Capacity ◽

Normal Cell Line

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Surface-enhanced Raman spectroscopic detection and differentiation of lung cancer cell lines (A549, H1229) and normal cell line (AT II) based on gold nanostar substrates

RSC Advances ◽

10.1039/c4ra07603f ◽

2014 ◽

Vol 4 (109) ◽

pp. 64225-64234 ◽

Cited By ~ 12

Author(s):

Man Wang ◽

Xiaowei Cao ◽

Wenbo Lu ◽

Lin Tao ◽

Hang Zhao ◽

...

Keyword(s):

Lung Cancer ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Normal Cell ◽

Lung Cancer Cell ◽

Normal Cell Line ◽

Raman Spectroscopic ◽

Surface Enhanced ◽

Surface Enhanced Raman

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Integrative proteomic and phosphoproteomic profiling of prostate cell lines

10.1101/696450 ◽

2019 ◽

Author(s):

Maria Katsogiannou ◽

Jean-Baptiste Boyer ◽

Alberto Valdeolivas ◽

Elisabeth Remy ◽

Laurence Calzone ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Line ◽

Cell Lines ◽

Differentially Expressed ◽

Migration And Invasion ◽

Mass Spectrometry Experiment ◽

Disease Etiology ◽

Prostate Cell ◽

Functional Features ◽

Prostate Cell Lines

ABSTRACTBackgroundProstate cancer is a major public health issue, mainly because patients relapse after androgen deprivation therapy. Proteomic strategies, aiming to reflect the functional activity of cells, are nowadays among the leading approaches to tackle the challenges not only of better diagnosis, but also of unraveling mechanistic details related to disease etiology and progression.MethodsWe conducted here a large SILAC-based Mass Spectrometry experiment to map the proteomes and phosphoproteomes of four widely used prostate cell lines, namely PNT1A, LNCaP, DU145 and PC3, representative of different cancerous and hormonal status.ResultsWe identified more than 3000 proteins and phosphosites, from which we quantified more than 1000 proteins and 500 phosphosites after stringent filtering. Extensive exploration of this proteomics and phosphoproteomics dataset allowed characterizing housekeeping as well as cell-line specific proteins, phosphosites and functional features of each cell line. In addition, by comparing the sensitive and resistant cell lines, we identified protein and phosphosites differentially expressed in the resistance context. Further data integration in a molecular network highlighted the differentially expressed pathways, in particular migration and invasion, RNA splicing, DNA damage repair response and transcription regulation.ConclusionsOverall, this study proposes a valuable resource toward the characterization of proteome and phosphoproteome of four widely used prostate cell lines and reveals candidates to be involved in prostate cancer progression for further experimental validation.

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