scholarly journals High-glucose induced HIF-1α down-regulation impairs the function of the endothelial progenitor cells via PI3K/AKT signaling pathway

2020 ◽  
Vol 6 (4) ◽  
Author(s):  
Yanting Dong ◽  
Xiaohui Zhou ◽  
Song Zhang ◽  
Xihua Lin ◽  
Nan Zhang
Keyword(s):  
Progenitor Cells ◽  
Signaling Pathway ◽  
Endothelial Progenitor Cells ◽  
High Glucose ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Endothelial Progenitor ◽  
Down Regulation

Interleukin-1 Beta Increases Activity of Human Endothelial Progenitor Cells: Involvement of PI3K-Akt Signaling Pathway

Inflammation ◽  
2012 ◽  
Vol 35 (4) ◽  
pp. 1242-1250 ◽  
Author(s):  
Lin Yang ◽  
Xiao-Gang Guo ◽  
Chang-Qing Du ◽  
Jin-Xiu Yang ◽  
Dong-Mei Jiang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Signaling Pathway ◽  
Endothelial Progenitor Cells ◽  
Interleukin 1 ◽  
Akt Signaling ◽  
Interleukin 1 Beta ◽  
Akt Signaling Pathway ◽  
Endothelial Progenitor

scholarly journals Dronedarone hydrochloride enhances the bioactivity of endothelial progenitor cells via regulation of the AKT signaling pathway

2021 ◽  
Vol 25 (5) ◽  
pp. 459-466
Author(s):  
Jian Zhang ◽  
Thi Hong Van Le ◽  
Vinoth Kumar Rethineswaran ◽  
Yeon-Ju Kim ◽  
Woong Bi Jang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Signaling Pathway ◽  
Endothelial Progenitor Cells ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Endothelial Progenitor

scholarly journals Occludin downregulation in high glucose is regulated by SSTR2 via the VEGF/NRP1/Akt signaling pathway in RF/6A cells

2017 ◽  
Vol 14 (2) ◽  
pp. 1732-1738 ◽  
Author(s):  
Mengling Li ◽  
Shuaiwei Wang ◽  
Songjiang Wang ◽  
Lei Zhang ◽  
Dongdong Wu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
High Glucose ◽  
Akt Signaling ◽  
Akt Signaling Pathway

scholarly journals Cinnamic hydroxamic acid inhibits the proliferation of gastric cancer cells via upregulation of miR 145 expression and down-regulation of P13K/Akt signaling pathway

2020 ◽  
Vol 19 (5) ◽  
pp. 957-963
Author(s):  
ShanPing Li ◽  
SenMao Hu
Keyword(s):  
Gastric Cancer ◽  
Signaling Pathway ◽  
Hydroxamic Acid ◽  
Akt Signaling ◽  
Migration And Invasion ◽  
Akt Signaling Pathway ◽  
Down Regulation ◽  
Protein Expressions ◽  
Dose Dependent ◽  
Cinnamic Hydroxamic Acid

Purpose: To investigate the anti-proliferative effect of cinnamic hydroxamic acid (CHA) on gastric cancer (GC) cells, and its mechanism of action.Methods: Two GC cell lines (SGC-7901 and MKN1) and normal human gastric epithelial cells (GES1) were used for this study. The GC cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM)supplemented with 10 % fetal bovine serum (FBS) and 1 % penicillin/streptomycin solution at 37 °C for 24 h in a humidified atmosphere of 5 % CO2 and 95 % air. GES1 cells were cultured in RPMI medium supplemented with 10 % FBS only. Cell viability and apoptosis were determined using 3 (4,5 dimethyl thiazol 2 yl) 2,5 diphenyl 2H tetrazolium bromide (MTT), and flow cytometric assays, respectively. The level of expression of microRNA-145 (miR-145) was determined using real-time quantitative polymerase chain reaction (qRT-PCR). Protein expressions of c-Myc, p-AKT, PI3K, p21, and matrix metalloproteinase (MMP)-2 and MMP-9were determined using Western blotting.Results: Treatment of GC cells with CHA for 72 h led to significant and dose-dependent reduction in their viability, and significant and dose-dependent increase in the number of apoptotic cells (p < 0.05). It also significantly arrested GC cell cycle at G1 phase (p < 0.05). The treatment significantly and dosedependently decreased SGC-7901 and MKN1 cell migration and invasion, and upregulated miR-145 mRNA expression (p < 0.05). The expression of miR-145 mRNA was significantly higher in MKN1 cells than in SGC-7901cells (p < 0.05). Treatment of SGC-7901 and MKN1 cells with CHA significantly downregulated protein expressions of c-Myc, MMP-2/9, PI3K and p-AKT, but upregulated p21 protein expression (p< 0.05).Conclusion: These results show that CHA inhibits the proliferation of GC cells via upregulation of miR-145 expression and down-regulation of  P13K/Akt signaling pathway. Therefore, CHA has a good potential as a therapeutic agent for the management of gastric cancer Keywords: Apoptosis, Cinnamic hydroxamic acid, Gastric cancer, Metastasis, Proliferation

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