scholarly journals Resident Cardiac Stem Cells on Fibrous Poly (L-lactide) Patch Augment Cardiac Repair via in-patch Differentiation and Simultaneous Migration into Infarcted Heart Tissue

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Heiwon Kyung ◽  
Heejung Kim ◽  
Hye-Jin Chung ◽  
Seung Jin Lee ◽  
In Kyong Shim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Heart Tissue ◽  
Cardiac Repair ◽  
Cardiac Stem Cells ◽  
Infarcted Heart

scholarly journals Local activation of cardiac stem cells for post-myocardial infarction cardiac repair

2012 ◽  
Vol 16 (11) ◽  
pp. 2549-2563 ◽  
Author(s):  
Zhuzhi Wen ◽  
Zun Mai ◽  
Haifeng Zhang ◽  
Yangxin Chen ◽  
Dengfeng Geng ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cells ◽  
Cardiac Repair ◽  
Post Myocardial Infarction ◽  
Cardiac Stem Cells ◽  
Local Activation

scholarly journals INCREASED POTENCY OF CARDIAC STEM CELLS COMPARED TO BONE MARROW MESENCHYMAL STEM CELLS IN CARDIAC REPAIR

2011 ◽  
Vol 57 (14) ◽  
pp. E1014
Author(s):  
Behzad Nasehi Oskouei ◽  
Guillaume Lamirault ◽  
Chacko Joseph ◽  
Stephanie Landa ◽  
Marc Dauer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cardiac Repair ◽  
Cardiac Stem Cells ◽  
Marrow Mesenchymal Stem Cells

OVER-EXPRESSION OF SDF1α ENHANCES CARDIAC REPAIR BY CARDIAC STEM CELLS

2014 ◽  
Vol 30 (10) ◽  
pp. S185
Author(s):  
E.L. Tilokee ◽  
R. Jackson ◽  
A. Mayfield ◽  
N. Latham ◽  
B. Ye ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cardiac Repair ◽  
Cardiac Stem Cells ◽  
Over Expression

Abstract 1471: Development and Characterization of Force Generating Human Engineered Heart Tissue from Embryonic Stem Cells

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Christina Rogge ◽  
Michael Didié ◽  
Erich Wettwer ◽  
Ursula Ravens ◽  
Ralph Graichen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Heart Tissue ◽  
Cardiac Repair ◽  
Embryoid Bodies ◽  
Laser Scanning Microscopy ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Engineered Heart Tissue

Engineered Heart Tissue (EHT) from neonatal rat cardiomyocytes has been used successfully as in vitro model and in cardiac repair. Here, we hypothesized that human embryonic stem cells (hESC) can be used to generate EHT with properties of native myocardium. Methods: hESC (hES3-ENVY) were differentiated in embryoid bodies, enzymatically dispersed, and subjected to EHT-generation in circular casting molds (1.5x10 6 cells, 0.4 mg collagen, 10% Matrigel/EHT; inner/outer diameter - 2/4 mm). Contractile function was assessed 10 days after casting under isometric conditions (37°C, 1.5 Hz, Tyrode’s solution). Action potentials (AP) were recorded in spontaneously contracting EHTs with intracellular electrodes (37°C, Tyrode’s solution). Calcium gradients were assessed by confocal laser scanning microscopy (CLSM) after rhod-2 loading. EHT-morphology was examined by CLSM and electron microscopy (EM). Results: hESC-EHTs contracted synchronously and spontaneously at 1.1±0.1 Hz (n=3). Increasing concentrations of extracellular calcium (0.2–2.4 mM) enhanced force of contraction from 53±8 to 199±22 μN (n=8, p<0.05; EC 50 : 0.8±0.04 mM). Isoprenaline (1 μM) at 0.4 mM calcium increased twitch tension from 61±7 to 108±15 μN (n=8, p<0.05) and shortened relaxation time from 111±6 to 87±4 ms (n=3, p<0.05). Cardiomyocytes within EHTs formed a functional syncytium composed of predominantly oriented muscle strands with a high degree of sarcomere differentiation (CLSM, EM). Cell-cell contacts through adherens junctions were identified by EM. Synchronous calcium gradient spread in spontaneously contracting EHTs indicated electrical coupling of individual cells within the multicellular constructs. AP recordings identified pacemaker cells (spontaneous diastolic depolarization) and cells with a flat phase 4 of the AP (working myocardium-like cells). Pharmacological studies demonstrated the presence and functional relevance of I Na (10–30 μM flecainide), I Ca (1 μM nisoldipine), and I Kr (1–5 μM E4031). Conclusion: Human force-generating EHT with functional and morphological properties of native myocardium can be generated. Ultimately, hESC-EHTs may constitute a model system for substance screening and could further be utilized in cardiac repair.

Abstract 15747: Over-Expression of Insulin-Like Growth Factor-1 by Human Cardiac Stem Cells Enhances Long-Term Retention of Transplanted Cells While Promoting Salvage of Injured Myocardium

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Robyn Jackson ◽  
Everad L Tilokee ◽  
Nicholas Latham ◽  
Bin Ye ◽  
Munir Boodhwani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Annexin V ◽  
Cardiac Repair ◽  
Population Doubling ◽  
Cardiac Stem Cells ◽  
Insulin Like Growth Factor ◽  
Over Expression ◽  
Myocardial Apoptosis

Background: Insulin-like growth factor (IGF-1) is a potent pro-survival cytokine that is not robustly expressed by human cardiac stem cells (CSCs). Previously, we have shown that paracrine engineering of CSCs with IGF-1 improves cell-mediated cardiac repair. Here, we explore the mechanisms underlying IGF-1 enhanced cardiac repair by CSCs. Methods/Results: Sub-culture of isolated c-Kit+, CD90+ and lineage negative cells (c-Kit-/CD90-) demonstrated that the natural low level production of IGF-1 by CSCs (149±16 pg/ml*mg) is secreted by all 3 sub-populations. After culture in hypoxic reduced serum media, lentiviral mediated over-expression of IGF-1 enhanced proliferation (population doubling time: 1.4±1.7 vs.-0.9±1.2 and -1.9±2.4 days, respectively; p≤0.01), expression of pro-survival transcripts (AKT, ERK and MAPK pathways) and pro-survival proteins (Bcl-2, Bcl-x, HIF-1a; p≤0.01) while decreasing expression of apoptotic markers (3.5±0.9 and 3.7±0.9 fold less annexin V; p≤0.01) as compared to GFP- and non-transduced CSCs. The high expression of the IGF-1 (79±3%) or the insulin receptor (61±5%) on CSCs suggests that autocrine pro-survival pre-conditioning underlies these effects. Direct and indirect co-culture of CSCs with neonatal rat ventricular cardiomyocytes (NRVMs) within hypoxic conditions demonstrated that IGF-1 promoted indirect myocardial repair by increasing NRVM viability and pro-survival signaling (Bcl2+; p≤0.01) while reducing apoptosis (annexin V+; p≤0.05) as compared GFP- or non-transduced CSCs. Transplant of CSCs genetically engineered to over-express IGF-1 into immunodeficient mice one week after infarction boosted IGF-1 content within infarcted tissue by 2.9±0.2 fold (p=0.004) and long-term engraftment (+4 weeks human alu content increased by 9.1±4 fold; p=0.05) while reducing myocardial apoptosis (3.4±0.3 and 2.5±0.5 fold reduction expression of Bax and p53, respectively; p<0.05) and long-term myocardial scarring (+ 4 weeks 2.2±0.4 fold less; p=0.01) as compared to GFP-transduced CSCs. Conclusions: Transplantation of IGF-1 enriched CSCs enhances cardiac repair by boosting transplant cell survival and reducing myocardial apoptosis to improve myocardial function and salvage of damaged myocardium.

scholarly journals Isolation of human explant derived cardiac stem cells from cryopreserved heart tissue

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0176000 ◽  
Author(s):  
Robyn Jackson ◽  
Seth Mount ◽  
Bin Ye ◽  
Audrey E. Mayfield ◽  
Vincent Chan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Heart Tissue ◽  
Cardiac Stem Cells

scholarly journals Another angiogenesis-independent role for VEGF: SDF1-dependent cardiac repair via cardiac stem cells

2011 ◽  
Vol 91 (3) ◽  
pp. 369-370 ◽  
Author(s):  
F. Claes ◽  
W. Vandevelde ◽  
L. Moons ◽  
M. Tjwa
Keyword(s):  
Stem Cells ◽  
Cardiac Repair ◽  
Cardiac Stem Cells

scholarly journals Haemodynamic unloading increases the survival and affects the differentiation of cardiac stem cells after implantation into an infarcted heart

2014 ◽  
Vol 45 (6) ◽  
pp. 976-982 ◽  
Author(s):  
H. Kurazumi ◽  
T.-S. Li ◽  
Y. Takemoto ◽  
R. Suzuki ◽  
A. Mikamo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cardiac Stem Cells ◽  
Infarcted Heart

Cardiac Stem Cells to Cure Heart Through Nanotechnology

2020 ◽  
Author(s):  
Prithiv K R Kumar
Keyword(s):  
Stem Cells ◽  
Embryonic Stem ◽  
Underlying Mechanism ◽  
Heart Tissue ◽  
Vital Role ◽  
Heart Cells ◽  
Cardiac Stem Cells ◽  
Sustainable Environment ◽  
Heart Repair ◽  
Key Issues

Loss of heart cells lead to heart attack. The blood flow slows down, eventually pumping gets effected. Several surgeries only weaken the heart. The solution to heart trouble is rather underlying mechanism to derive into regeneration. Several clinical trials have highlighted that stem cells have a promising effect in regeneration. More methods such as cardiac stem cells, bone marrow based cells, mesenchyme stem cells; embryonic stem cells have been effective to an extent. There have been some inconsistencies as well in these methods. Performance of heart has lowered; efficiency to pump blood has influence on selection of type of stem cells. However, future of therapies has to rely on technology. Not just ordinary technology, but nanotechnology. The future of heart repair and advancement of stem cells to cure any heart disease. Nanotechnology helps in understanding cellular layers of heart tissue, which plays a vital role for any heart repair or regeneration. This review discusses the aspects of nanoparticles and nanogel effect in curing heart. Nanoparticles have been effective in drug delivery, targeting particular area of cure. Meanwhile nanogel helps in navigating the drugs and creating a sustainable environment for treatment. Another concept highlighted in this review is nanopatch along with nano material or nanocells communication, helping in regenerative therapy. Thus key issues for future prospects are discussed below.

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