scholarly journals Clinical Significance of microRNA Polymorphisms and Expression Profiles in Oral Cancer Development

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Kaiser Jamil
Keyword(s):  
Oral Cancer ◽  
Clinical Significance ◽  
Expression Profiles ◽  
Cancer Development

scholarly journals Human Papillomavirus 16 Oncoproteins Downregulate the Expression of miR-148a-3p, miR-190a-5p, and miR-199b-5p in Cervical Cancer

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Mi-Soon Han ◽  
Jae Myun Lee ◽  
Soo-Nyung Kim ◽  
Jae-Hoon Kim ◽  
Hyon-Suk Kim
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
High Risk ◽  
Expression Profiles ◽  
Normal Group ◽  
Cancer Development ◽  
Hybridization Method ◽  
Cancer Group ◽  
Cervical Cancer Development ◽  
High Risk Hpv

Almost all cervical cancers are associated with human papillomavirus (HPV); however, the majority of women infected with this virus do not develop cervical cancer. Therefore, new markers are needed for reliable screening of cervical cancer, especially in relation to HPV infection. We aimed to identify potential microRNAs that may serve as diagnostic markers for cervical cancer development in high-risk HPV-positive patients. We evaluated the microRNA expression profiles in 12 cervical tissues using the hybridization method and verified them by quantitative polymerase chain reaction (qPCR). Finally, we evaluated the effects of HPV16 oncoproteins on the expression of selected microRNAs using cervical cancer cells (CaSki and SiHa) and RNA interference. With the hybridization method, eight microRNAs (miR-9-5p, miR-136-5p, miR-148a-3p, miR-190a-5p, miR-199b-5p, miR-382-5p, miR-597-5p, and miR-655-3p) were found to be expressed differently in the HPV16-positive cervical cancer group and HPV16-positive normal group (fold change ≥ 2). The results of qPCR showed that miR-148a-3p, miR-190a-5p, miR-199b-5p, and miR-655-3p levels significantly decreased in the cancer group compared with the normal group. Upon silencing of HPV16 E5 and E6/E7, miR-148a-3p levels increased in both cell lines. Silencing of E6/E7 in SiHa cells led to the increase in miR-199b-5p and miR-190a-5p levels. Three HPV16 oncoproteins (E5, E6, and E7) downregulate miR-148a-3p, while E6/E7 inhibit miR-199b-5p and miR-190a-5p expression in cervical carcinoma. The three microRNAs, miR-148a-3p, miR-199b-5p, and miR-190a-5p, may be novel diagnostic biomarkers for cervical cancer development in high-risk HPV-positive patients.

scholarly journals Association between expression of Carboxypeptidase 4 and stem cell markers and their clinical significance in liver cancer development

2017 ◽  
Vol 8 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Lichao Sun ◽  
Chunguang Guo ◽  
Joseph Burnett ◽  
Jian Pan ◽  
Zhihua Yang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Cancer ◽  
Clinical Significance ◽  
Cancer Development ◽  
Stem Cell Markers ◽  
Cell Markers

scholarly journals The Regulatory Roles of MicroRNAs in Bone Remodeling and Perspectives as Biomarkers in Osteoporosis

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Mengge Sun ◽  
Xiaoya Zhou ◽  
Lili Chen ◽  
Shishu Huang ◽  
Victor Leung ◽  
...  
Keyword(s):  
Cardiovascular Diseases ◽  
Bone Loss ◽  
Bone Remodeling ◽  
Expression Profiles ◽  
Pathological Process ◽  
Cancer Development ◽  
Tissue Formation ◽  
Research Groups ◽  
Novel Biomarkers ◽  
Serum Micrornas

MicroRNAs are involved in many cellular and molecular activities and played important roles in many biological and pathological processes, such as tissue formation, cancer development, diabetes, neurodegenerative diseases, and cardiovascular diseases. Recently, it has been reported that microRNAs can modulate the differentiation and activities of osteoblasts and osteoclasts, the key cells that are involved in bone remodeling process. Meanwhile, the results from our and other research groups showed that the expression profiles of microRNAs in the serum and bone tissues are significantly different in postmenopausal women with or without fractures compared to the control. Therefore, it can be postulated that microRNAs might play important roles in bone remodeling and that they are very likely to be involved in the pathological process of postmenopausal osteoporosis. In this review, we will present the updated research on the regulatory roles of microRNAs in osteoblasts and osteoclasts and the expression profiles of microRNAs in osteoporosis and osteoporotic fracture patients. The perspective of serum microRNAs as novel biomarkers in bone loss disorders such as osteoporosis has also been discussed.

scholarly journals Exploring lay public and dental professional knowledge around HPV transmission via oral sex and oral cancer development

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Mario A. Brondani ◽  
Adriana B. Siqueira ◽  
Claudia Maria Coelho Alves
Keyword(s):  
Human Papillomavirus ◽  
Oral Cancer ◽  
Health Care Professionals ◽  
Oropharyngeal Cancer ◽  
Hpv Infection ◽  
Cancer Development ◽  
Oral Sex ◽  
Sexual Activities ◽  
Lay Public ◽  
Dental Professionals

Abstract Background Human papillomavirus (HPV) has been associated with certain types of oropharyngeal cancers and yet, the level of knowledge that dental professionals and the lay public have in terms of HPV transmission, oral sexual activities, and oral cancer development needs exploration. The aim of this study was to assess the knowledge held by practicing dental professionals as well as the lay public regarding Human Papillomavirus (HPV) transmission through oral sex and subsequent oropharyngeal cancer development. Methods Textual data were collected from a public forum with dental professionals in. Vancouver, who discussed the HPV-oral sex-oral cancer triad, and from survey data gathered from 212 lay public participants (also in Vancouver) who answered a 13-item questionnaire on the perceived risks of oral sex in terms of HPV infection and oropharyngeal cancer development. The data were analyzed statistically by age group, gender, and sexual orientation using descriptive statistics, while an ANOVA test was used to compare variation in the responses to the survey (p-value = 0.05). Results The forum engaged 46 health care professionals, many of whom were aware of the potential risks for head and neck cancer development due to HPV infection, while also questioning “how to effectively talk about HPV with patients.” The survey revealed that 34.5% of the participants believed that oral sex is an activity with no or low risk for the transmission of HPV, while 84% of participants believed the same sexual practices were of low or no-risk for HIV (Human Immunodeficiency Virus) transmission. Most participants (82%) never discussed oral sexual activities with their physicians or dentists/dental hygienists. Conclusions The general public remains mostly unaware of the potential links between HPV infection and oropharyngeal cancer. Physicians and dental providers should discuss oral sexual practice with their patients to raise awareness.

scholarly journals New DNA Methylation Markers and Global DNA Hypomethylation Are Associated with Oral Cancer Development

2015 ◽  
Vol 8 (11) ◽  
pp. 1027-1035 ◽  
Author(s):  
Jean-Philippe Foy ◽  
Curtis R. Pickering ◽  
Vassiliki A. Papadimitrakopoulou ◽  
Jaroslav Jelinek ◽  
Steven H. Lin ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Oral Cancer ◽  
Cancer Development ◽  
Dna Hypomethylation

Abstract 3091: Oncogenic roles of nuclear VCP in oral cancer development

2010 ◽  
Author(s):  
I-Wen Chiu ◽  
Chien-Feng Li ◽  
Chien-Chen Lai ◽  
Ming-Tsung Lai ◽  
Chih-Mei Chen ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Development

scholarly journals MUC1, MUC2, MUC5AC, and MUC6 in colorectal cancer: expression profiles and clinical significance

2016 ◽  
Vol 469 (3) ◽  
pp. 255-265 ◽  
Author(s):  
Johannes Betge ◽  
Nora I. Schneider ◽  
Lars Harbaum ◽  
Marion J. Pollheimer ◽  
Richard A. Lindtner ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Clinical Significance ◽  
Expression Profiles

Connecting gene expression subtypes of colorectal cancer (CRC) with cell lines and drug resistance.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e14544-e14544
Author(s):  
Eva Budinska ◽  
Jenny Wilding ◽  
Vlad Calin Popovici ◽  
Edoardo Missiaglia ◽  
Arnaud Roth ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Clinical Significance ◽  
Cell Lines ◽  
Drug Response ◽  
Drug Sensitivity ◽  
Expression Profiles ◽  
Enrichment Analysis ◽  
Gene Set Enrichment Analysis

e14544 Background: We identified CRC gene expression subtypes (ASCO 2012, #3511), which associate with established parameters of outcome as well as relevant biological motifs. We now substantiate their biological and potentially clinical significance by linking them with cell line data and drug sensitivity, primarily attempting to identify models for the poor prognosis subtypes Mesenchymal and CIMP-H like (characterized by EMT/stroma and immune-associated gene modules, respectively). Methods: We analyzed gene expression profiles of 35 publicly available cell lines with sensitivity data for 82 drug compounds, and our 94 cell lines with data on sensitivity for 7 compounds and colony morphology. As in vitro, stromal and immune-associated genes loose their relevance, we trained a new classifier based on genes expressed in both systems, which identifies the subtypes in both tissue and cell cultures. Cell line subtypes were validated by comparing their enrichment for molecular markers with that of our CRC subtypes. Drug sensitivity was assessed by linking original subtypes with 92 drug response signatures (MsigDB) via gene set enrichment analysis, and by screening drug sensitivity of cell line panels against our subtypes (Kruskal-Wallis test). Results: Of the cell lines 70% could be assigned to a subtype with a probability as high as 0.95. The cell line subtypes were significantly associated with their KRAS, BRAF and MSI status and corresponded to our CRC subtypes. Interestingly, the cell lines which in matrigel created a network of undifferentiated cells were assigned to the Mesenchymal subtype. Drug response studies revealed potential sensitivity of subtypes to multiple compounds, in addition to what could be predicted based on their mutational profile (e.g. sensitivity of the CIMP-H subtype to Dasatinib, p<0.01). Conclusions: Our data support the biological and potentially clinical significance of the CRC subtypes in their association with cell line models, including results of drug sensitivity analysis. Our subtypes might not only have prognostic value but might also be predictive for response to drugs. Subtyping cell lines further substantiates their significance as relevant model for functional studies.

AGING AND ORAL CANCER DEVELOPMENT

2021 ◽  
Vol 132 (1) ◽  
pp. e32
Author(s):  
S Niklander ◽  
D Bandaru ◽  
DW Lambert ◽  
KD Hunter
Keyword(s):  
Oral Cancer ◽  
Cancer Development
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