scholarly journals Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes

2019 ◽  
Vol 6 (1) ◽  
pp. 65-101 ◽  
Author(s):  
Hannah L Klein ◽  
Kenny K.H. Ang ◽  
Michelle R. Arkin ◽  
Emily C. Beckwitt ◽  
Yi-Hsuan Chang ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Recombination ◽  
Repair Processes

Modulation of DNA repair processes by arsenic and selenium compounds

Toxicology ◽  
2003 ◽  
Vol 193 (1-2) ◽  
pp. 161-169 ◽  
Author(s):  
A. Hartwig ◽  
H. Blessing ◽  
T. Schwerdtle ◽  
I. Walter
Keyword(s):  
Dna Repair ◽  
Selenium Compounds ◽  
Repair Processes

scholarly journals Host Cell DNA Repair Pathways in Adeno-Associated Viral Genome Processing

2006 ◽  
Vol 80 (21) ◽  
pp. 10346-10356 ◽  
Author(s):  
Vivian W. Choi ◽  
Douglas M. McCarty ◽  
R. Jude Samulski
Keyword(s):  
Dna Repair ◽  
Dna Recombination ◽  
Recq Helicase ◽  
Cellular Mechanisms ◽  
Adeno Associated Virus ◽  
Mrn Complex ◽  
Dependent Vector ◽  
Cellular Dna ◽  
Dna Maintenance

ABSTRACT Recentstudies have shown that wild-type and recombinant adeno-associated virus (AAV and rAAV) genomes persist in human tissue predominantly as double-stranded (ds) circular episomes derived from input linear single-stranded virion DNA. Using self-complementary recombinant AAV (scAAV) vectors, we generated intermediates that directly transition to ds circular episomes. The scAAV genome ends are palindromic hairpin-structured terminal repeats, resembling a double-stranded break repair intermediate. Utilizing this substrate, we found cellular DNA recombination and repair factors to be essential for generating circular episomal products. To identify the specific cellular proteins involved, the scAAV circularization-dependent vector was used as a reporter in 19 mammalian DNA repair-deficient cell lines. The results show that RecQ helicase family members (BLM and WRN), Mre11 and NBS1 of the Mre11-Rad50-Nbs1 (MRN) complex, and ATM are required for efficient scAAV genome circularization. We further demonstrated that the scAAV genome requires ATM and DNA-PKCS, but not NBS1, to efficiently convert to a circular form in nondividing cells in vivo using transgenic mice. These studies identify specific pathways involved for further elucidating viral and cellular mechanisms of DNA maintenance important to the viral life cycle and vector utilizations.

recA-dependent DNA repair processes

BioEssays ◽  
1989 ◽  
Vol 10 (1) ◽  
pp. 12-16 ◽  
Author(s):  
Kendric C. Smith ◽  
Tzu-Chien V. Wang
Keyword(s):  
Dna Repair ◽  
Repair Processes

DNA repair processes are critical mediators of p53-dependent tumor suppression

2018 ◽  
Vol 24 (7) ◽  
pp. 947-953 ◽  
Author(s):  
Ana Janic ◽  
Liz J. Valente ◽  
Matthew J. Wakefield ◽  
Leon Di Stefano ◽  
Liz Milla ◽  
...  
Keyword(s):  
Dna Repair ◽  
Tumor Suppression ◽  
Repair Processes

Is Ap4A involved in DNA repair processes?

1988 ◽  
Vol 177 (1) ◽  
pp. 143-153 ◽  
Author(s):  
G. Gilson ◽  
J.P. Ebel ◽  
P. Remy
Keyword(s):  
Dna Repair ◽  
Repair Processes
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