scholarly journals The dual role of cyclin C connects stress regulated gene expression to mitochondrial dynamics

2014 ◽  
Vol 1 (10) ◽  
pp. 318-324 ◽  
Author(s):  
Randy Strich ◽  
◽  
Katrina Cooper
Keyword(s):  
Gene Expression ◽  
Mitochondrial Dynamics ◽  
Dual Role ◽  
Cyclin C ◽  
Regulated Gene Expression

scholarly journals E2F factors rate controls the dual role of CDE/E2F composite element: A model of E2F-regulated gene expression in plant development

FEBS Letters ◽  
2006 ◽  
Vol 580 (22) ◽  
pp. 5167-5171 ◽  
Author(s):  
Frédéric Lincker ◽  
Mélanie Messmer ◽  
Guy Houlné ◽  
Martine Devic ◽  
Marie-Edith Chabouté
Keyword(s):  
Gene Expression ◽  
Plant Development ◽  
Dual Role ◽  
Composite Element ◽  
Regulated Gene Expression

scholarly journals Role of a COP1 Interactive Protein in Mediating Light-Regulated Gene Expression in Arabidopsis

1998 ◽  
Vol 10 (7) ◽  
pp. 1083-1094 ◽  
Author(s):  
Yoshiharu Y. Yamamoto ◽  
Minami Matsui ◽  
Lay-Hong Ang ◽  
Xing-Wang Deng
Keyword(s):  
Gene Expression ◽  
Regulated Gene Expression

scholarly journals Role of metadherin in estrogen-regulated gene expression

2017 ◽  
Vol 40 (2) ◽  
pp. 303-310
Author(s):  
Yujun Li ◽  
Jesus Gonzalez Bosquet ◽  
Shujie Yang ◽  
Kristina W. Thiel ◽  
Yuping Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulated Gene Expression

scholarly journals Role of Nuclear Factor-κB in the Antiviral Action of Interferon and Interferon-regulated Gene Expression

2004 ◽  
Vol 279 (30) ◽  
pp. 31304-31311 ◽  
Author(s):  
Lawrence M. Pfeffer ◽  
Jong-Gwan Kim ◽  
Susan R. Pfeffer ◽  
Dennis J. Carrigan ◽  
Darren P. Baker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Antiviral Action ◽  
Regulated Gene Expression

scholarly journals An Improved Method for the Study of Apoptosis-Related Genes Using the Tet-On System

2011 ◽  
Vol 16 (3) ◽  
pp. 332-337 ◽  
Author(s):  
Marc W. Halterman
Keyword(s):  
Gene Expression ◽  
Caspase 3 ◽  
Functional Characterization ◽  
Continuous Exposure ◽  
Genetic Screens ◽  
Pulse Dose ◽  
Target Effect ◽  
Regulated Gene Expression

Inducible gene expression systems are particularly useful for the functional characterization of genes with putative toxic properties. In the course of studying the role of hypoxia-regulated gene expression on cell survival using the tetracycline-inducible (tet-on) system, the author noted that exposure to the inducing ligand doxycycline (dox) inhibited caspase-3 cleavage in control samples. To limit this confounding off-target effect, he devised an in vitro pulse dose, delayed-injury protocol testing both dox and a novel tetracycline analog 9-t-butyl doxycycline (9-TB). Although 9-TB induced higher transgene levels compared to matched concentrations of dox, continuous exposure to both drugs inhibited caspase-3 cleavage in hypoxic samples. Conversely, a 6-h pulse dose of 9-TB followed by a 40-h washout period prior to hypoxic challenge activated robust transgene expression and lessened the inhibitory effects on caspase-3 processing. It is anticipated that these protocol modifications will improve the performance of tet-regulated genetic screens, particularly in situations where cell death is used as a primary end point.

scholarly journals A toxin-antitoxin system associated transcription factor of Caulobacter crescentus can influence cell cycle-regulated gene expression during the SOS response

2020 ◽  
Author(s):  
Koyel Ghosh ◽  
Kamilla Ankær Brejndal ◽  
Clare L. Kirkpatrick
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Dna Damage ◽  
Transcription Factor ◽  
Caulobacter Crescentus ◽  
Sos Response ◽  
Cell Cycle Gene ◽  
Regulated Gene Expression ◽  
Cell Cycle Gene Expression

AbstractToxin-antitoxin (TA) systems are widespread in bacterial chromosomes but their functions remain enigmatic. Although many are transcriptionally upregulated by stress conditions, it is unclear what role they play in cellular responses to stress and to what extent the role of a given TA system homologue varies between different bacterial species. In this work we investigate the role of the DNA damage-inducible TA system HigBA of Caulobacter crescentus in the SOS response and discover that in addition to the toxin HigB affecting cell cycle gene expression through inhibition of the master regulator CtrA, HigBA possesses a transcription factor third component, HigC, which both auto-regulates the TA system and acts independently of it. Through HigC, the system exerts downstream effects on antibiotic (ciprofloxacin) resistance and cell cycle gene expression. HigB and HigC had inverse effects on cell cycle gene regulation, with HigB reducing and HigC increasing the expression of CtrA-dependent promoters. Neither HigBA nor HigC had any effect on formation of persister cells in response to ciprofloxacin. Rather, their role in the SOS response appears to be as transcriptional and post-transcriptional regulators of cell cycle-dependent gene expression, transmitting the status of the SOS response as a regulatory input into the cell cycle control network via CtrA.ImportanceAlmost all bacteria respond to DNA damage by upregulating a set of genes that helps them to repair and recover from the damage, known as the SOS response. The set of genes induced during the SOS response varies between species, but frequently includes toxin-antitoxin systems. However, it is unknown what the consequence of inducing these systems is, and whether they provide any benefit to the cells. We show here that the DNA damage-induced TA system HigBA of the asymmetrically dividing bacterium Caulobacter crescentus affects the cell cycle regulation of this bacterium. HigBA also has a transcription factor encoded immediately downstream of it, named here HigC, which controls expression of the TA system and potentially other genes as well. Therefore, this work identifies a new role for TA systems in the DNA damage response, distinct from non-specific stress tolerance mechanisms which had been proposed previously.

scholarly journals Cilium Expression Score Predicts Glioma Survival

2021 ◽  
Vol 12 ◽  
Author(s):  
Srinivas Rajagopalan ◽  
Amartya Singh ◽  
Hossein Khiabanian
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Brain Regions ◽  
Poor Survival ◽  
Aggressive Tumors ◽  
Regulated Gene Expression ◽  
Upregulated Genes ◽  
Expression Score

The accurate classification, prognostication, and treatment of gliomas has been hindered by an existing cellular, genomic, and transcriptomic heterogeneity within individual tumors and their microenvironments. Traditional clustering is limited in its ability to distinguish heterogeneity in gliomas because the clusters are required to be exclusive and exhaustive. In contrast, biclustering can identify groups of co-regulated genes with respect to a subset of samples and vice versa. In this study, we analyzed 1,798 normal and tumor brain samples using an unsupervised biclustering approach. We identified co-regulated gene expression profiles that were linked to proximally located brain regions and detected upregulated genes in subsets of gliomas, associated with their histologic grade and clinical outcome. In particular, we present a cilium-associated signature that when upregulated in tumors is predictive of poor survival. We also introduce a risk score based on expression of 12 cilium-associated genes which is reproducibly informative of survival independent of other prognostic biomarkers. These results highlight the role of cilia in development and progression of gliomas and suggest potential therapeutic vulnerabilities for these highly aggressive tumors.

scholarly journals Zinc and Traumatic Brain Injury: From Chelation to Supplementation

2020 ◽  
Vol 8 (3) ◽  
pp. 36 ◽  
Author(s):  
Cathy W. Levenson
Keyword(s):  
Gene Expression ◽  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Incidence Rate ◽  
Molecular Mechanisms ◽  
Behavioral Deficits ◽  
Zinc Accumulation ◽  
Regulated Gene Expression

With a worldwide incidence rate of almost 70 million annually, traumatic brain injury (TBI) is a frequent cause of both disability and death. Our modern understanding of the zinc-regulated neurochemical, cellular, and molecular mechanisms associated with TBI is the result of a continuum of research spanning more than three decades. This review describes the evolution of the field beginning with the initial landmark work on the toxicity of excess neuronal zinc accumulation after injury. It further shows how the field has expanded and shifted to include examination of the cellular pools of zinc after TBI, identification of the role of zinc in TBI-regulated gene expression and neurogenesis, and the use of zinc to prevent cognitive and behavioral deficits associated with brain injury.

Dual role of TTC5 cofactor in GR-mediated gene expression

2013 ◽  
pp. 1-1
Author(s):  
Malihah Sadeq ◽  
Constantinos Demonacos ◽  
Marija KrsticDemonacos
Keyword(s):  
Gene Expression ◽  
Dual Role
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