scholarly journals Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae

2014 ◽  
Vol 1 (9) ◽  
pp. 303-314 ◽  
Author(s):  
Antonio Rego ◽  
◽  
Ana Duarte ◽  
Flavio Azevedo ◽  
Maria Sousa ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Death ◽  
Cell Wall ◽  
Acetic Acid ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

scholarly journals Deletion of Atg22 gene contributes to reduce programmed cell death induced by acetic acid stress in Saccharomyces cerevisiae

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Jingjin Hu ◽  
Yachen Dong ◽  
Wei Wang ◽  
Wei Zhang ◽  
Hanghang Lou ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Cell Death ◽  
Cell Wall ◽  
Acetic Acid ◽  
Programmed Cell Death ◽  
Cell Wall Integrity ◽  
Yeast Cells ◽  
Death Process ◽  
Lignocellulosic Biofuel

Abstract Background Programmed cell death (PCD) induced by acetic acid, the main by-product released during cellulosic hydrolysis, cast a cloud over lignocellulosic biofuel fermented by Saccharomyces cerevisiae and became a burning problem. Atg22p, an ignored integral membrane protein located in vacuole belongs to autophagy-related genes family; prior study recently reported that it is required for autophagic degradation and efflux of amino acids from vacuole to cytoplasm. It may alleviate the intracellular starvation of nutrition caused by Ac and increase cell tolerance. Therefore, we investigate the role of atg22 in cell death process induced by Ac in which attempt is made to discover new perspectives for better understanding of the mechanisms behind tolerance and more robust industrial strain construction. Results In this study, we compared cell growth, physiological changes in the absence and presence of Atg22p under Ac exposure conditions. It is observed that disruption and overexpression of Atg22p delays and enhances acetic acid-induced PCD, respectively. The deletion of Atg22p in S. cerevisiae maintains cell wall integrity, and protects cytomembrane integrity, fluidity and permeability upon Ac stress by changing cytomembrane phospholipids, sterols and fatty acids. More interestingly, atg22 deletion increases intracellular amino acids to aid yeast cells for tackling amino acid starvation and intracellular acidification. Further, atg22 deletion upregulates series of stress response genes expression such as heat shock protein family, cell wall integrity and autophagy. Conclusions The findings show that Atg22p possessed the new function related to cell resistance to Ac. This may help us have a deeper understanding of PCD induced by Ac and provide a new strategy to improve Ac resistance in designing industrial yeast strains for bioethanol production during lignocellulosic biofuel fermentation.

Necrotic and apoptotic cell death induced by Captan on Saccharomyces cerevisiae

2017 ◽  
Vol 33 (8) ◽  
Author(s):  
Fernando J. Scariot ◽  
Luciane Jahn ◽  
Ana Paula L. Delamare ◽  
Sergio Echeverrigaray
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

The fungicide Mancozeb induces metacaspase-dependent apoptotic cell death in Saccharomyces cerevisiae BY4741

APOPTOSIS ◽  
2016 ◽  
Vol 21 (7) ◽  
pp. 866-872 ◽  
Author(s):  
F. J. Scariot ◽  
L. M. Jahn ◽  
J. P. Maianti ◽  
A. P. L. Delamare ◽  
S. Echeverrigaray
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

Citral and geraniol induce necrotic and apoptotic cell death on Saccharomyces cerevisiae

2021 ◽  
Vol 37 (3) ◽  
Author(s):  
Fernando J. Scariot ◽  
Mariliza S. Pansera ◽  
Ana Paula L. Delamare ◽  
Sergio Echeverrigaray
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death
Sign in / Sign up

Export Citation Format

Share Document