scholarly journals Experimental study of the antibacterial activity of the lytic Staphylococcus aureus bacteriophage ph20 and lytic Pseudomonas aeruginosa bacteriophage ph57 during modelling of its impregnation into poly(methylmetacrylate) orthopedic implants (bone cement)

2018 ◽  
Vol 73 (1) ◽  
pp. 59-68 ◽  
Author(s):  
A. G. Samokhin ◽  
Ju. N. Kozlova ◽  
D. V. Korneev ◽  
O. S. Taranov ◽  
E. A. Fedorov ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bone Cement ◽  
Polymethyl Methacrylate ◽  
Bacterial Colonization ◽  
Experimental Studies ◽  
Orthopedic Implants ◽  
Polymerization Process ◽  
Polymerization Reaction ◽  
Chemical Components

Background: The problem of bacterial colonization of implants used in medical practice continues to be relevant regardless of the material of the implant. Particular attention deserves polymeric implants, which are prepared ex tempore from polymethyl methacrylate, for example - duting orthopedic surgical interventions (so-called "bone cement"). The protection of such implants by antibiotic impregnation is subjected to multiple criticisms, therefore, as an alternative to antibiotics, lytic bacteriophages with a number of unique advantages can be used - however, no experimental studies have been published on the possibility of impregnating bacteriophages into polymethyl methacrylate and their antibacterial activity assessment under such conditions.Aims: to evaluate the possibility of physical placement of bacteriophages in polymethylmethacrylate and to characterize the lytic antibacterial effect of two different strains of bacteriophages when impregnated into polymer carrier ex tempore during the polymerization process in in vitro model.Materials and methods:  First stage - Atomic force microscopy (AFM) of polymethyl methacrylate samples for medical purposes was used to determine the presence and size of caverns in polymethyl methacrylate after completion of its polymerization at various reaction  temperatures (+6…+25°C and +18…+50°C).The second stage was performed in vitro and included an impregnation of two different bacteriophage strains (phage ph20 active against S. aureus and ph57 active against Ps. aeruginosa) into polymethyl methacrylate during the polymerization process, followed by determination of their antibacterial activity.Results: ACM showed the possibility of bacteriophages placement in the cavities of polymethyl methacrylate - the median of the section and the depth of cavities on the outer surface of the polymer sample polymerized at +18…+50°C were 100.0 and 40.0 nm, respectively, and on the surface of the transverse cleavage of the sample - 120.0 and 100.0 nm, respectively, which statistically did not differ from the geometric dimensions of the caverns of the sample polymerized at a temperature of +6…+25°C.The study of antibacterial activity showed that the ph20 bacteriophage impregnated in polymethyl methacrylate at +6…+25°C lost its effective titer within the first six days after the start of the experiment, while the phage ph57 retained an effective titer for at least 13 days.Conclusion: the study confirmed the possibility of bacteriophages impregnation into medical grade polymethyl methacrylate, maintaining the effective titer of the bacteriophage during phage emission into the external environment, which opens the way for the possible application of this method of bacteriophage delivery in clinical practice. It is also assumed that certain bacteriophages are susceptible to aggressive influences from the chemical components of "bone cement" and / or polymerization reaction products, which requires strict selection of bacteriophage strains that could be suitable for this method of delivery.

scholarly journals Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan

2020 ◽  
Vol 10 (18) ◽  
pp. 6528 ◽  
Author(s):  
Mayra Eliana Valencia Zapata ◽  
José Herminsul Mina Hernandez ◽  
Carlos David Grande Tovar ◽  
Carlos Humberto Valencia Llano ◽  
Blanca Vázquez-Lasa ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Bone Cement ◽  
Orthopedic Surgery ◽  
Bending Strength ◽  
Orthopedic Implants ◽  
Parietal Bone ◽  
Maximum Temperature ◽  
Cement Interface

Acrylic bone cement (ABC) is one of the most used materials in orthopedic surgery, mainly for the fixation of orthopedic implants to the bone. However, ABCs usually present lack of biological activity and osseointegration capacity that leads to loosening of the prosthesis. This work reports the effect of introducing graphene oxide (GO) and chitosan (CS), separately or together, in the ABC formulation on setting performance, mechanical behavior, and biological properties. Introduction of both CS and GO to the ABC decreased the maximum temperature by 21% and increased the antibacterial activity against Escherichia coli by 87%, while introduction of only CS decreased bending strength by 32%. The results of cell viability and cell adhesion tests showed in vitro biocompatibility. The in vivo response was investigated using both subdermal and bone parietal implantations in Wistar rats. Modified ABCs showed absence of immune response, as confirmed by a normal inflammatory response in Wistar rat subdermal implantation. The results of the parietal bone implantation showed that the addition of CS and GO together allowed a near total healing bone–cement interface, as observed in the micrographic analysis. The overall results support the great potential of the modified ABCs for application in orthopedic surgery mainly in those cases where osseointegration is required.

scholarly journals An In vitro Assessment of Antibacterial Activity of Three Self-etching Primers Against Oral Microflora

2017 ◽  
Vol 7 ◽  
pp. 181-187
Author(s):  
Sneha Dipak Shinde ◽  
Vikram Pai ◽  
R. Vijay Naik
Keyword(s):  
Antibacterial Activity ◽  
Growth Inhibition ◽  
Bacterial Colonization ◽  
Brain Heart Infusion ◽  
Dilution Method ◽  
Filter Paper Disc ◽  
Oral Microflora ◽  
Agar Diffusion Test ◽  
Serial Dilution Method

Aims This study aims to evaluate and compare the antibacterial activity of three self-etching primers (SEP), namely, Transbond plus, Reliance, and Gluma against commonly encountered oral microflora (Streptococcus mutans, Lactobacillus acidophilus, and Actinomyces viscosus). Subjects and Methods The antibacterial activity of the three SEPs was examined against microorganisms using agar diffusion test (ADT) and minimum inhibitory concentration (MIC). In ADT, Whatman’s filter paper disc of 5 mm was loaded with primer and polymerized. This was placed on previously inoculated brain heart infusion and blood agar plates and was incubated for 48– 72 h at 37°C according to the microorganism. For assessing MIC serial dilution method was used. Statistical Analysis Used Data were analyzed with Kruskal–Wallis (P < 0.001) and Mann–Whitney tests. Results Only Transbond plus SEP and Reliance SEP produced a clear growth inhibition halo against S. mutans, L. acidophilus and A. viscosus. Gluma SEP did not show any growth inhibition halo against S. mutans, L. acidophilus, and A. viscosus. Conclusions TSEP and Reliance SEP did show antibacterial activity in an in vitro environment. Therefore, this study concludes that the use of these SEPs may contribute to a reduction in bacterial colonization.

Effects of Stem Design and Pre-Cooling Prostheses on the Heat Generated by Bone Cement in an In Vitro Model

2002 ◽  
Vol 30 (3) ◽  
pp. 265-270 ◽  
Author(s):  
O Rodop ◽  
A Kiral ◽  
O Arpacioglu ◽  
I Akmaz ◽  
C Solakoglu ◽  
...  
Keyword(s):  
Bone Cement ◽  
In Vitro Model ◽  
Hip Prosthesis ◽  
Polymerization Process ◽  
Temperature Decrease ◽  
Stem Design ◽  
Temperature Changes ◽  
Cement Interface ◽  
Vitro Model

The necrotizing effects of the heat, particularly at more than 50 °C, produced by the exothermic polymerization process associated with the acrylic implant cement polymethylmethacrylate (PMMA) are well documented. The temperature changes that occur are dependent on the thickness of the PMMA. The current study investigates the hypothesis that the heat produced by the bone cement may be reduced by the choice of stem design and by pre-cooling the hip prosthesis. The thermal alterations at the bone-cement interface were measured in an in vitro model. The results indicated that a temperature decrease of approximately 7 °C could be achieved by pre-cooling the prosthesis, and by changing the shape of the prosthesis stem from flat and wide to round.

scholarly journals In vitro antimicrobial and cytotoxic activity of Lippia origanoides essential oil against bacteria of potential health concern

2021 ◽  
Author(s):  
Edwin Stiven Quiguanás Guarín ◽  
Juan Pablo Bedoya Agudelo ◽  
Jhon Esteban Lopez-Carvajal ◽  
Yuly Andrea Ramírez Tabares ◽  
Leonardo Padilla Sanabria ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Natural Products ◽  
Essential Oil ◽  
Vero Cells ◽  
In Vitro Cytotoxicity ◽  
Health Concern ◽  
Chemical Components ◽  
Potential Health ◽  
Major Chemical

Due to the growing resistance they develop of bacteria to drugs, the search for alternatives in natural products is considered important such as Lippia origanoides essential oil. Here, the antibacterial activity of the oil and two of its major chemical components were tested against bacteria of potential health concern. The cytotoxicity of these compounds was evaluated in human erythrocytes and Vero cells. 51 compounds were identified in the LOEO, being terpinen-4-ol, γ-Terpinene, citronellal and thymol the main. LOEO and thymol showed antibacterial activity from 904 μg/mL and 200 μg/mL, respectively. γ-Terpinene did not show activity any concentration tested. LOEO showed hemolysis at concentration of 3000 μg/mL and thymol at 100 μg/mL. LOEO and thymol showed cytotoxicity in the evaluated cell lines at 250 μg/mL and 100 μg/mL, respectively. These compounds have a moderate cytotoxicity so it's considered necessary to study alternatives to reduce the in vitro cytotoxicity of these compounds.

Thespesia populnea: An Ethnomedicinal, Phytochemical and Pharmacological review

2020 ◽  
Vol 10 ◽  
Author(s):  
Ashish Vishwakarma ◽  
Poonam Arora ◽  
Mahaveer Dhobi
Keyword(s):  
Therapeutic Potential ◽  
Biological Activities ◽  
Experimental Studies ◽  
Bioactive Molecules ◽  
Chemical Components ◽  
Coastal Forests ◽  
Thespesia Populnea ◽  
Wide Range

: Thespesia populnea, family, Malvaceae, commonly known as paras pipal and Indian tulip tree, is widely distributed in coastal forests of India and south-eastern areas. The plant is medicinally used for the treatment of numerous diseases including cutaneous infections, brain and liver disorders.The review summarizes all the information related to botanical characteristics, traditional uses, chemical components and biological activities of T. populnea, in order to exploit therapeutic potential of this plant.x.The available information about T. populnea was collected through the online search on Web of Science, PubMed, Science Direct, Springer and Google Scholar. T. populnea is widely explored concerning its phytochemistry and biological activities. Amongst all phytoconstituents present in Thespesia, sesquiterpenes and phenolic compounds are major bioactive ingredients in plant. Experimental studies show that these compounds exhibit a wide range of biological activities including anti-inflammatory, antidiabetic, analgesic, wound healing, anti-alzheimer, anti-ulcer and anti-psoriasis in in vitro and in vivo animal studies.To sum up, the plant, T. populnea, possess high medicinal and social value, that deserves further investigation. T. populnea is promising plant to be utilized in the development of pharmaceutical drug products. However, there is a lack of scientific studies to confirm its ethnopharmcological uses. In addition, further studies on isolation of bioactive molecules and their pharmacological studies are recommended that could be of great significance towards clinical application of this plant.

In vitro evaluation of bone cements impregnated with selenium nanoparticles stabilized by phosphatidylcholine (PC) for application in bone

2020 ◽  
Vol 35 (3) ◽  
pp. 385-404
Author(s):  
Zeynep Karahaliloglu ◽  
Ebru Kilicay
Keyword(s):  
Bone Cement ◽  
Cell Line ◽  
Bacterial Colonization ◽  
Antioxidant Properties ◽  
Osteogenic Sarcoma ◽  
Selenium Nanoparticles ◽  
Bone Cements ◽  
Graft Material ◽  
Osteoblastic Cell Line

One of the most common prophylactic techniques to solve prosthetic joint infection (PJI) is incorporation of antibiotics into acrylic bone cement to prevent bacterial colonization and proliferation by providing local antibiotic delivery directly at the implant site. Further, there has been a significant concern over the efficacy of commonly used antibiotics within bone cement due to the rise in multi-drug resistant (MDR) microorganisms. Selenium is an essential trace element that has multiple beneficial effects for human health and its chemotherapeutic action is well known. It was reported that nanostructured selenium enhanced bone cell adhesion and has an increased osteoblast function. In this context, we used the selenium nanoparticles (SeNPs) to improve antibacterial and antioxidant properties of poly (methyl methacrylate) (PMMA) and tri calcium phosphate (TCP)-based bone cements, and to reduce of the infection risk caused by orthopedic implants. As another novelty of this study, we proposed phosphatidylcholine (PC) as a unique and natural stabilizer in the synthesis of selenium nanoparticles. After the structural analysis of the prepared bone cements was performed, in vitro osteointegration and antibacterial efficiency were tested using MC3T-E1 (mouse osteoblastic cell line) and SaOS-2 (human primary osteogenic sarcoma) cell lines, and S. aureus (Gram positive) and E.coli (Gram negative) strains, respectively. More importantly, PC-SeNPs-reinforced bone cements exhibited significant effect against E. coli, compared to S. aureus and a dose-dependent antibacterial activity against both bacterial strains tested. Meanwhile, these bone cements induced the apoptosis of SaOS-2 through increased reactive oxygen species without negatively influencing the viability of the healthy cell line. Furthermore, the obtained confocal images revealed that PC-SeNPs (103.7 ± 0.56 nm) altered the cytoskeletal structure of SaOS-2 owing to SeNPs-induced apoptosis, when MC3T3-E1 cells showed a typical spindle-shaped morphology. Taken together, these results highlighted the potential of PC-SeNPs-doped bone cements as an effective graft material in bone applications.

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY OF CHALCONE DERIVATIVES FROM Kaempferia pandurata RHIZOMES AGAINST CLINICAL ISOLATE BACTERIA

2017 ◽  
Vol 13 (1) ◽  
pp. 41 ◽  
Author(s):  
Soerya Dewi Marliyana ◽  
Yana Maolana Syah ◽  
Didin - Mujahidin
Keyword(s):  
Antibacterial Activity ◽  
Clinical Isolate ◽  
Enterobacter Aerogenes ◽  
Salmonella Typhi ◽  
Chemical Components ◽  
Chalcone Derivatives ◽  
Microdilution Method ◽  
In Vitro Antibacterial Activity ◽  
Vacuum Liquid Chromatography

<p>In vitro antibacterial activity of chalcone derivatives from “temu kunci” (<em>K. pandurta</em>) rhizomes against clinical isolate bacteria has been done. Two chalcone derivatives, panduratin A (<strong>1</strong>) and 4-hydroxypanduratin A (<strong>2</strong>) were isolated from <em>Kaempferia pandurata</em> rhizomes. Isolation of the chemical components were done with extraction (maceration), vacuum liquid chromatography and radial chromatography methods. The structures were determined by NMR spectroscopy (<sup>1</sup>H-NMR, <sup>13</sup>C-NMR, 1D and 2D), then compare with data from literatures. Antibacterial activity was carried out with reference to the CLSI microdilution method, against eight clinical isolate bacteria such as <em>Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysentriae </em>and<em> Vibrio cholerae.</em> Compounds <strong>1</strong> and <strong>2</strong> showed significant antibacterial activity with highest activity against <em>S. aureus</em> and <em>B. subtilis</em> with MIC values of 2.4 to 18.8 µg/mL and MBC values of 4.8 to 37.5 µg/mL. These results showed these compounds as potential antibacterial agent for clinical isolate bacteria.</p>

scholarly journals Phytochemical and antibacterial screening of different fractions of root part of Ipomea Turpethum

2018 ◽  
Vol 17 (1) ◽  
pp. 93-97
Author(s):  
Tehmina Sohail ◽  
Sadia Ferheen ◽  
Hina Imran ◽  
Zahra Yaqeen ◽  
Atiq Ur Rehman ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Medical Science ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Chemical Components ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Antibacterial Screening

Objective: In folk medicines, different herbs and plants have been used for many thousands of years .Now it is important to investigate these plants and herbs scientifically which have been used in traditional medicines. The aim of this study was to investigate the antibacterial activity and preliminary phytochemical screening of root of Ipomea turpethum extracted in methanol and its fractions.Methods: The methanol extract was further extracted with three solvents ethyl acetate, chloroform and hexane and analyzed for their antibacterial activity using by agar well diffusion method. They were tested against six bacteria; Echrichia coli, Staphylococcus aureus, Staphylococcus epidermidus, Proteus vulgaris, Pseudomonas auroginosa and Salmonella typhi. The susceptibility of microorganisms to all three fractions was compared with each other and with standard antibotic (Ampicillin). The fractions of Ipomea turpethum was also qualitatively analyzed for the presence of chemical components, i.e. saponins, alkaloids, flavonoids, tannins and glycosides.Result: Among all fractions methanol exhibited highest antibacterial activity (average zone of inhibition 23.53mm ± 1.3) while ethyl acetate exhibited least antibacterial activity (average zone of inhibition 18.50mm ± 3.5). Minimum inhibitory concentration of methanol, ethyl acetate, chloroform and hexane fractions was found in the range of 650ug/ml to 2500ugl/ml against microorganisms.Conclusion: Results obtained from this preliminary in-vitro experiment indicate that, all three fractions of Ipomea turpethum has good antibacterial activity against all microorganisms used. By phytochemical analysis of extract, it has been found to contain some nutrient and chemical components which support its ethenomedicinal use but further work is required for development of new antibiotic compounds.Bangladesh Journal of Medical Science Vol.17(1) 2018 p.93-97

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