scholarly journals Environmental Exposure to Endocrine Disruptor of Bisphenol A and Semen Quality of Men

2018 ◽  
Vol 73 (5) ◽  
pp. 338-343
Author(s):  
Stanislav V. Chigrinets ◽  
Gennady V. Brukhin
Keyword(s):  
Reproductive Health ◽  
Male Infertility ◽  
Bisphenol A ◽  
Seminal Fluid ◽  
Concentration Level ◽  
Semen Quality ◽  
Roc Curves ◽  
Total Testosterone ◽  
Idiopathic Male Infertility

Background: The reproductive health disorders in men are one of the urgent problems of international medicine. The prevalence of idiopathic male infertility has the highest rate. Oxidative stress, genetic factor, and endocrine disruptors are considered to be the most probable causes for the idiopathic male infertility. In this regard, studying the effect of endocrine disruptors, in particular bisphenol A on male reproductive health, becomes actual and relevant. Aims: The aim of the study was to reveal the relationship between the concentration level of bisphenol A (BPA) in seminal fluid and semen quality in men with normo- or pathozoospermia, as well as between the concentration level of bisphenol A and the level of total testosterone and estradiol in plasma. Materials and methods: 53 samples of seminal fluid of men with normo- or pathoospermia were studied. In seminal fluid the concentration of bisphenol A was determined by gas chromatography with mass spectrometry (GC-MS). The spermiological analysis was performed according to the WHO recommendations (2010) including the evaluation of sperm count/concentration, motility and morphology, and DNA fragmentation index. In addition, the concentration of total testosterone and estradiol in plasma was determined. The results were statistically processed using the Mann–Whitney U test, the correlation analysis, the paired regression method, and the ROC curves to determine the cut-off point for BPA in the seminal fluid. The results were considered statistically significant at p0.05. Results: In 100% of the ejaculate samples BPA with a median concentration of 0.15 (0.06–0.31) ng/ml was detected. Using the Spearman rank correlation coefficient, statistically significant correlations between the concentration of BPA and the total count (r=-0.330, p=0.016), concentration (r=-0.309; p=0.024), the proportion of progressively motile spermatozoa (r=-0.575; p=0.001), the proportion of spermatozoa with normal morphology (r=-0.397, p=0.003), the degree of sperm DNA fragmentation (r=0.349, p=0.025), and the concentration of total testosterone (r =-0.616; p0.001) were registered. A statistically significant inverse linear relationship (r=-0.406, p=0.003) and (r =-0.364, p=0.048) was determined by a paired linear regression between the BPA concentration in the seminal fluid and the proportion of progressively motile spermatozoa, and the total testosterone level respectively. To assess the risk of pathozoospermia, the threshold value of seminal BPA concentration was determined using the analysis of ROC-curves; the cut-off point was 0.1025 ng/ml. Conclusions: BPA in the seminal fluid influences negatively on the quality of the sperm and suppress the level of total testosterone in plasma.

scholarly journals Coenzyme Q10 and Male Infertility: A Systematic Review

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 874
Author(s):  
Gianmaria Salvio ◽  
Melissa Cutini ◽  
Alessandro Ciarloni ◽  
Lara Giovannini ◽  
Michele Perrone ◽  
...  
Keyword(s):  
Systematic Review ◽  
Male Infertility ◽  
Coenzyme Q10 ◽  
Seminal Fluid ◽  
Semen Quality ◽  
Antioxidant Properties ◽  
Semen Parameters ◽  
Optimal Dosage ◽  
Idiopathic Male Infertility ◽  
Coq10 Supplementation

Infertility affects 15% of couples worldwide. A male factor is involved in 50% of cases. The etiology of male infertility is poorly understood, but there is evidence for a strong association between oxidative stress (OS) and poor seminal fluid quality. For this reason, therapy with antioxidants is one of the cornerstones of empirical treatment of male infertility. Coenzyme Q10 (CoQ10)—an essential cofactor for energy production with major antioxidant properties—is commonly used to support spermatogenesis in idiopathic male infertility. This systematic review aims to elucidate the usefulness of CoQ10 supplementation in the treatment of male infertility, particularly with regard to semen quality assessed by conventional and advanced methods, and pregnancy rates. All studies report a beneficial effect of CoQ10 supplementation on semen parameters, although randomized controlled trials are a minority. Moreover, the optimal dosage of CoQ10 or how it can be combined with other antioxidant molecules to maximize its effect is unknown. However, CoQ10 is still one of the most promising molecules to treat idiopathic male infertility and warrants further investigation.

scholarly journals Environmental exposure to endocrine disruptors on semen quality of men

2018 ◽  
Vol 19 (3) ◽  
pp. 42-47
Author(s):  
S. V. Chigrinets ◽  
G. V. Bryuhin
Keyword(s):  
Bisphenol A ◽  
Dna Fragmentation ◽  
Endocrine Disruptors ◽  
Semen Quality ◽  
World Health ◽  
Plasma Testosterone ◽  
Total Testosterone ◽  
Study Objective ◽  
Normal Morphology

The study objective is to investigate the correlations between endocrine disruptors (bisphenol A, triclosan and 4-nonylphenol) exposure, measured on semen and semen quality of men, total testosterone in the plasma.Materials and methods. We measured semen bisphenol A, triclosan and 4-nonylphenol concentrations of 41 men by gas chromatography/ mass spectrometry. According to the World Health Organization guidelines, we divided our patients into 2 groups: normozoospermia and patozoospermia. And furthermore, total plasma testosterone and index DNA fragmentation spermatozoa were determined. The results were statistically processed using the Mann–Whitney U-test and correlation analysis. A p-value less than 0.05 was considered significant.Results. Bisphenol A was detected in 100 % of semen samples, triclosan – in 85,4 %, 4-nonylphenol – in 97,6 %, with a median concentration of 0.108, 0.17 and 0.165 ng/ml respectively. The groups were different statistically significant for levels of bisphenol A, triclosan and index DNA fragmentation spermatozoa (р <0.001, р = 0.033, р = 0.005 respectively). We found significant negative correlations between concentration of triclosan and percentage of normal morphology (p = 0.004), percentage of progressively motile spermatozoa (p = 0.049) and the positive correlation – between concentration of triclosan and percentage of DNA fragmentation spermatozoa (p <0,001). Аlso we found significant negative correlations between concentration of bisphenol A and total count (p = 0.04), percentage of progressively motile spermatozoa (p <0.001), percentage of normal morphology (p = 0.002), levels of total testosterone in plasma (p = 0.047). We demonstrated the synergy of investigated endocrine disruptors on semen quality.Conclusion. Semen bisphenol A and triclosan concentrations have a negative correlation with the parameters of the ejaculate. Environmental levels of bisphenol A negatively correlated with testosterone levels in plasma.

scholarly journals Connection of urethral microbiota with the semen quality and the concentration of endocrine disrupters in men semen

2018 ◽  
Vol 19 (4) ◽  
pp. 60-66
Author(s):  
S. V. Chigrinets ◽  
G. V. Bryuhin
Keyword(s):  
Bisphenol A ◽  
Seminal Fluid ◽  
Semen Quality ◽  
Statistical Processing ◽  
Test System ◽  
P Value ◽  
Study Objective ◽  
Comparison Groups

The study objective is to investigate the correlations between opportunistic microorganisms of the urethra and the quality of ejaculate, as well as the concentration of ubiquitory endocrine disruptors (bisphenol A and triclosan) in men semen.Materials and methods. We analysed 63 semen samples of men with normo- and patozoospermia. In seminal fluid the concentration of bisphenol A and triclosan was determined by gas chromatography with mass spectrometry. The microbiocenosis of the urethra was studied by real-time polymerase chain reaction using the Androflor test system. The results were subjected to statistical processing using the Mann — Whitney U-test and correlation analysis. A p-value less than 0.05 was considered significant.Results. Bisphenol A was found in 100 % of the semen samples. Triclosan was detected in 84.9 % of the samples. Comparison groups on the quality of ejaculate (normo- and pathozoospermia) were statistically significantly different in the concentration of bisphenol A and triclosan in seminal fluid (p <0.001) and (p = 0.003) respectively. The study established statistically significant correlations between bisphenol A concentration, seminal triclosan and urethral microbiota: Lactobacillus spp. (r = 0.501, p = 0.003), Corynebacterium spp. (r = 0.425, p = 0.015), Anaerococcus spp. (r = 0.371, p = 0.045), Eubacterium spp. (r = 0.357, p = 0.037) for bisphenol A and Staphylococrns spp. (r = 0.392, p = 0.026), Streptococcus spp. (r = —0.486, p = 0.005) for triclosan.Conclusion. In this study the connection of the urethral microbiota with the quality of the ejaculate and the concentration of endocrine dis-ruptors (bisphenol A and triclosan) in men semen was established. In this regard, it is necessary to take into account the role of asymptomatic urethral dysbiosis when administering patients with poor ejaculate quality.

scholarly journals Endocrine Disruption of Androgenic Activity by Perfluoroalkyl Substances: Clinical and Experimental Evidence

2018 ◽  
Vol 104 (4) ◽  
pp. 1259-1271 ◽  
Author(s):  
Andrea Di Nisio ◽  
Iva Sabovic ◽  
Umberto Valente ◽  
Simone Tescari ◽  
Maria Santa Rocca ◽  
...  
Keyword(s):  
Reproductive Health ◽  
Male Infertility ◽  
Experimental Evidence ◽  
Molecular Mechanisms ◽  
Nuclear Translocation ◽  
Seminal Fluid ◽  
Semen Quality ◽  
Substantial Impact ◽  
Gene Reporter Assay ◽  
Male Reproductive Health

Abstract Background Considerable attention has been paid to perfluoroalkyl compounds (PFCs) because of their worldwide presence in humans, wildlife, and environment. A wide variety of toxicological effects is well supported in animals, including testicular toxicity and male infertility. For these reasons, the understanding of epidemiological associations and of the molecular mechanisms involved in the endocrine-disrupting properties of PFCs on human reproductive health is a major concern. Objective To investigate the relationship between PFC exposure and male reproductive health. Design This study was performed within a screening protocol to evaluate male reproductive health in high schools. Patients This is a cross-sectional study on 212 exposed males from the Veneto region, one of the four areas worldwide heavily polluted with PFCs, and 171 nonexposed controls. Main Outcome Measures Anthropometrics, seminal parameters, and sex hormones were measured in young males from exposed areas compared with age-matched controls. We also performed biochemical studies in established experimental models. Results We found that increased levels of PFCs in plasma and seminal fluid positively correlate with circulating testosterone (T) and with a reduction of semen quality, testicular volume, penile length, and anogenital distance. Experimental evidence points toward an antagonistic action of perfluorooctanoic acid on the binding of T to androgen receptor (AR) in a gene reporter assay, a competition assay on an AR-coated surface plasmon resonance chip, and an AR nuclear translocation assay. Discussion This study documents that PFCs have a substantial impact on human health as they interfere with hormonal pathways, potentially leading to male infertility.

scholarly journals Once-a-day Tadalafil administration improves the spermogram parameters in fertile patients

2015 ◽  
Vol 87 (3) ◽  
pp. 210 ◽  
Author(s):  
Antonio Corvasce ◽  
Giuseppe Albino ◽  
Teresa Leonetti ◽  
Agnese Filomena Buonomo ◽  
Ettore Cirillo Marucco
Keyword(s):  
Seminal Fluid ◽  
Semen Quality ◽  
Type A ◽  
Daily Treatment ◽  
Sperm Cells ◽  
Average Increase ◽  
Once Daily ◽  
Positive Effects ◽  
Semen Volume

Objectives: We explored the safety of Tadalafil once-daily treatment for 12 week and its effects on semen quality in a clinical subpopulation of men with psychogenic erectile dysfunction (ED). Patients and Methods: Twenty-seven men, between 19 and 35 years, unaware of their fertility status, suffering from psychogenic ED were enrolled. The examination of the seminal fluid was performed twice before administration of Tadalafil and twice after three months of continuous daily administration of Tadalafil 5 mg. The volume of the seminal fluid, the concentration of sperm, the concentration of sperm with type “a+b” motility, the concentration of sperm with type “a” motility, the concentration of normal sperm were taken into consideration. The results before treatment with Tadalafil (T0) and after 3 months of treatment (T3m) were compared. Results: The administration of once-daily Tadalafil 5mg, brings to an average increase of the total number of sperm cells, both total and fast motility (type a) and the percentage of nemasperms, and to an average increase of semen volume of only 0.41 ml. These quantitative and qualitative improvements of the seminal fluid resulted statistically significant as regard motility, nemasperm percentage and seminal fluid volume. No unespected safety findings were observed. Conclusions: Tadalafil administration improves the quality of sperm cells and seminal fluid: in particular motility, percentage of nemasperms and volume of seminal fluid. We emphasize the safety of the once-daily treatment with tadalafil 5mg and the positive effects on spermatogenesis.

scholarly journals Vegetarian food recommendation in the traditional Islamic teachings to increase the fertility of men

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Reza Dehghani Bidgoli
Keyword(s):  
Clinical Trials ◽  
Male Infertility ◽  
Seminal Fluid ◽  
Medical Science ◽  
Academic Research ◽  
Narrative Texts ◽  
Vegetable Food ◽  
New Knowledge ◽  
Effective Drugs

Abstract About half of the infertilities are due to men’s problems. In medicine, there are valuable treasures that confirm the effectiveness of some of them today and they have been approved for the treatment of diseases. The purpose of this paper is to investigate the drug’s advice in increasing the fertility of men in Muslim narrative texts and compare it with new knowledge of humanity in medicine. For this purpose, we searched the fertility, men, and vegetable food keywords in the database and valid Islamic books and presented them in this article. Also, the results of academic research have also been pointed out to confirm these religious teachings. Some of the issues in traditional Islamic teachings that have been expressed centuries ago have been confirmed by modern medical science and have been the basis of making effective drugs for treating male infertility. Some of them also need further research. According to the emphasis of available Islamic traditional medicine from the infallible on the efficacy of the vegetable food in improving the quality of the seminal fluid and also confirming the usefulness in clinical trials, these plants can be useful in increasing fertility.

scholarly journals Role of Kisspeptin Gene Polymorphism in Idiopathic Male Infertility in Iraq

2021 ◽  
pp. 3428-3435
Author(s):  
Firas Kareem Al-Kalabi ◽  
Adnan F Al-Azzawie ◽  
Estabraq AR. Al-Wasiti
Keyword(s):  
Male Infertility ◽  
Semen Quality ◽  
Control Group ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Idiopathic Male Infertility ◽  
Polymerase Chain ◽  
Infertile Group ◽  
Control Study

     This case control study aimed to determine single nucleotide polymorphisms (SNPs) in the Kisspeptin (KISS1) gene in males with idiopathic infertility and their association with sex hormones and semen quality. The study included a total of 60 infertile and 30 healthy fertile males. Our results show that the level of the measured hormones (LH, FSH, Testosterone, Prolactin and Kisspeptin-54) were higher in the control group than in the male infertile group at p<0.05. We used polymerase chain reaction restriction fragment length polymorphism (PCR-RELP) for the genotyping of KISS1 position rs35431622 (Q36R) KISS1, which showed three different genotypes of different sizes; a wild-type homozygous AA of 233 bp and a heterozygous AG that has digestion products of 233, 161, and 72 bp. The AG was more frequent in the patients group which also had high OR value of G allele (3.105). While for the rs4889 (C/G(, there was a correlation between the CC genotype and the patients group, but it was non-significant. Patients had an OR value of 2.5 for the CC genotype with 95% CI: 0.21 – 29.26, whereas the OR value for the C allele was 1.14 with 95% CI: 0.613 – 2.135. In conclusion, variations in SNPs of the KISS1 gene may be considered as a risk factor for idiopathic male infertility in Iraqi population.

scholarly journals Inhibin B and activin A in the pathogenesis of idiopathic male infertility

2015 ◽  
Vol 96 (5) ◽  
pp. 749-752
Author(s):  
E F Galimova ◽  
G Kh Akhmadullina ◽  
K V Bulygin ◽  
K S Mochalov ◽  
Sh N Galimov
Keyword(s):  
Male Infertility ◽  
Seminal Fluid ◽  
Biological Fluids ◽  
Regulatory Peptides ◽  
Activin A ◽  
Inhibin B ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reproductive Disorders ◽  
Idiopathic Male Infertility

Aim. To assess the role of serum and ejaculate inhibin-activin status disorders in the development of reproductive disorders at idiopathic male infertility. Methods. The research includes data of laboratory and clinical examination of 82 infertile and 60 fertile men. Serum and semen levels of reproduction regulatory peptides inhibin B and activin A were determined using standard commercially available kits for enzyme-linked immunosorbent assay. Other parameters of ejaculate were also investigated. Results. In men with infertility of the unknown cause, sperm cell concentration was significantly decreased, and the proportion of sex cells with morphological anomalies was increased. The main features of inhibin-activin profile of biological fluids in healthy males with proven fertility were revealed, which were the significant gradient of inhibin and activin intertissue concentrations and domination of these molecular fertility factors in seminal plasma, corresponding with the views of their key role in sperm fertilizing capacity. In patients with pathospermia, the significant decrease of inhibin B concentration in the ejaculate but not in serum, associated with increased activin A levels was revealed, accompanied by characteristic shift of inhibin-activin ratio associated with the deterioration of quality and quantity parameters of semen. Conclusion. Revealed changes in the inhibin-activin sperm plasma mirror may be a pathogenetic basis for the idiopathic infertility in couples. Inhibin-activin coefficient in blood serum and seminal fluid may be used as a promising diagnostic and prognostic marker of reproductive dysfunction risk.

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