Bone Formation in the Maxillary Sinus by Using Platelet-rich Plasma: An Experimental Study in Sheep

2005 ◽  
Vol 31 (1) ◽  
pp. 2-17 ◽  
Author(s):  
Edgar Grageda ◽  
Jaime L. Lozada ◽  
Phillip J. Boyne ◽  
Nicholas Caplanis ◽  
Paul J. McMillan
Keyword(s):  
Platelet Count ◽  
Bone Regeneration ◽  
Maxillary Sinus ◽  
Platelet Rich Plasma ◽  
Test Group ◽  
Bone Allograft ◽  
Autogenous Bone ◽  
Control Group ◽  
Freeze Dried ◽  
Log Ratio

Abstract Recently, platelet-rich plasma (PRP) has been proven to be an effective regeneration adjunct when combined with autogenous bone in the reconstruction of mandibular defects. However, little is known about the effect of PRP when combined with a bone allograft in the maxillary sinus. The purpose of this study was to quantitatively evaluate the ability of PRP to enhance bone regeneration in the maxillary sinus of sheep when combined with demineralized freeze-dried bone allograft (DFDBA) and cortical cancellous freeze-dried bone allograft (CCFDBA). Ten sheep were selected for bilateral sinus augmentation DFDBA + CCFDBA + PRP (test) and DFDBA + CCFDBA (control). Five were sacrificed at 3 months and the other 5 at 6 months. Hematology tests were performed for platelet count, and histology slides were obtained for histomorphometric analysis taking 2 measures of interest: total area (square millimeters) and percentage of bone fill. Student t tests showed no significant difference between test and control groups for total area (P > .25) and percentage of bone fill (P > .80) at either 3 or 6 months. The control group showed no statistical difference for total area (P < .095) and percentage of bone fill (P < .60) between 3- and 6-month healing times. The test group, however, showed a significant increase in total area (P < .025) but not in percentage of bone fill (P < .40) for the 2 healing periods. When the treatments were compared for interactions within the animal model, no clear tendency was evident for the test group to perform in relation to the control group regarding total area (r = .766, P < .01). A moderate tendency existed between the percentages of bone filled (r = .824, P < .005). Platelet-rich plasma showed higher platelet count than did the whole blood (2 to 5 times). However, no correlation was found between the log ratio and the bone measures. Within the limitations of this study, PRP failed to enhance or accelerate bone regeneration in the maxillary sinus of sheep when combined with bone allograft.

Efficacy of Platelet-Rich-Plasma (PRP) in Bone Regeneration after Cyst Enucleation in Pediatric Patients – A Clinical Study

2010 ◽  
Vol 35 (1) ◽  
pp. 81-87 ◽  
Author(s):  
NB Nagaveni ◽  
B Praveen Reddy ◽  
KV Umashankar ◽  
B Pranav ◽  
Sreedevi Reddy ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Pediatric Patients ◽  
Platelet Rich Plasma ◽  
Test Group ◽  
Control Group ◽  
Clinical Use ◽  
Group Control ◽  
Group Control Group ◽  
Regeneration Of Bone

Background: Platelet-rich-plasma (PRP) is a type of natural source of autologous growth factors, and has been used successfully in various fields. However, the use of PRP in children is not well documented. Objective:To evaluate the efficacy of adding PRP to a bone graft in the bone regeneration of cystic bony defects following cystectomy. Study design: Study sample included 20 children who were randomly divided into two groups with 10 patients in each group; all underwent cystectomy. In the first group (test group), after cystectomy the cystic defect was filled with PRP and bone graft. In the second group (control group) bone graft alone (without PRP) was used. Radiographs were recorded at 1 month, 2 months, 4 months and 6 months intervals after surgery to evaluate the defect bone fill in both groups. Results: The post-operative successive radiographs in the test group showed a significantly greater regeneration of bone in the height of bony defects with application of PRP to bone graft as compared to the control group. In the test group, by the first post operative month, about 58% of the defect was filled, which gradually increased in each month and showed about 94% of defect-fill by 6 months. In the control group, similar observation revealed only 31%of defect-fill by the first post-operative month and a 47% defect-fill at 6 months. Conclusion: The addition of PRP to bone graft appeared to enhance bone regeneration considerably. The combination of PRP and bone graft might have a potential for routine clinical use for regeneration of cystic bony defects in children.

scholarly journals Evaluation of the Histomorphometric and Micromorphometric Performance of a Serum Albumin-Coated Bone Allograft Combined with A-PRF for Early and Conventional Healing Protocols after Maxillary Sinus Augmentation: A Randomized Clinical Trial

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1810
Author(s):  
Bálint Trimmel ◽  
Szabolcs Gyulai-Gaál ◽  
Márton Kivovics ◽  
Noémi Piroska Jákob ◽  
Csaba Hegedűs ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Maxillary Sinus ◽  
Treatment Time ◽  
Test Group ◽  
Bone Allograft ◽  
Control Group ◽  
Two Stage ◽  
Sinus Augmentation ◽  
Maxillary Sinus Augmentation ◽  
Total Treatment Time

The aim of this study was to compare the microarchitecture of augmented bone following maxillary sinus augmentation (MSA) after healing periods of 3 (test) and 6 (control) months using the combination of advanced platelet-rich fibrin (A-PRF) and a serum albumin-coated bone allograft (SACBA). Twenty-six patients with 30 surgical sites who required two-stage MSA were enrolled and grafted with the combination of A-PRF and SACBAs. The surgical sites were randomly allocated to the test or control group. During implant site preparation, 17 bone core biopsy samples were collected from each study group for histological, histomorphometric and micromorphometric analysis. Resonance frequency analysis was performed at the time of implant placement and 6, 8, 10, and 12 weeks postoperatively. The percentage of newly formed bone was 44.89 ± 9.49% in the test group and 39.75 ± 8.15% in the control group (p = 0.100). The results of the µCT analysis showed no significant differences in morphometric parameters between the study groups. The implant stability quotient was not significantly different between the two groups at 10 and 12 weeks postoperatively. Based on these findings, the total treatment time may be reduced by 3 months with the use of A-PRF and SACBAs for two-stage MSA.

Bioglass Associated With Leukocyte-Poor Platelet-Rich Plasma in the Rabbit Maxillary Sinus: Histomorphometric, Densitometric, and Fractal Analysis

2010 ◽  
Vol 36 (5) ◽  
pp. 333-343 ◽  
Author(s):  
Ivy Kiemle Trindade-Suedam ◽  
Juliana Aparecida Najarro Dearo de Morais ◽  
Rafael Silveira Faeda ◽  
Fábio Renato Manzolli Leite ◽  
Guilherme Monteiro Tosoni ◽  
...  
Keyword(s):  
Fractal Dimension ◽  
Bone Density ◽  
Bone Formation ◽  
Maxillary Sinus ◽  
Platelet Rich Plasma ◽  
Autogenous Bone ◽  
New Bone Formation ◽  
Mean Values ◽  
Dimension Analysis ◽  
Fractal Dimension Analysis

Abstract The objective of the present study was to evaluate the outcomes of autogenous bone graft (AB) and bioglass (BG) associated or not with leukocyte-poor platelet-rich plasma (LP-PRP) in the rabbit maxillary sinus (MS) by histomorphometric and radiographic analysis. Twenty rabbits divided into 2 groups (G1, G2) were submitted to sinus lift surgery. In G1, 10 MS were grafted with AB and 10 MS were grafted with BG. In G2, 10 MS were grafted with AB + LP-PRP and 10 MS were grafted with BG + LP-PRP. After 90 days, the animals were killed and specimens were obtained, x-rayed, and submitted to histomorphometric, radiographic bone density (RD) and fractal dimension analysis. Radiographic bone density mean values (SD), expressed as aluminum equivalent in mm, of AB, BG, AB + LP-PRP, and BG + LP-PRP groups were 1.79 (0.31), 2.04 (0.39), 1.61 (0.28), and 1.53 (0.30), respectively. Significant differences (P < 0.05) were observed between BG and AB, and BG + PRP and BG. Fractal dimension mean values were 1.48 (0.04), 1.35 (0.08), 1.44 (0.04), and 1.44 (0.06), respectively. Significant differences were observed between BG and AB, and AB + LP-PRP and BG. Mean values for the percentage of bone inside MS were 63.30 (8.60), 52.65 (10.41), 55.25 (7.01), and 51.07 (10.25), respectively. No differences were found. No correlations were observed among percentage of bone, RD and FD. Histological analysis showed that MS treated with AB presented mature and new bone formation. The other groups showed minor bone formation. Within the limitations of this study, the results indicated that at a 90-day time end point, AB yielded better results than AB + LP-PRP, BG, and BG + LP-PRP and should be considered the primary material for MS augmentation.

scholarly journals The Human Amniotic Mesenchymal Stem Cells (hAMSCs) Improve the Implant Osseointegration and Bone Regeneration in Maxillary Sinus Floor Elevation in Rabbits

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Lu Yin ◽  
Zhi-xuan Zhou ◽  
Ming Shen ◽  
Ning Chen ◽  
Fei Jiang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Maxillary Sinus ◽  
Bone Volume ◽  
Control Group ◽  
Sinus Floor Elevation ◽  
Dental Implantation ◽  
Posterior Maxilla ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Maxillary Sinus Floor Elevation

Insufficient bone height in the posterior maxilla is a challenging problem in dental implantation. Bio-Oss, though routinely used in maxillary sinus floor elevation (MSFE), is not osteoinductive. Human amniotic mesenchymal cells (hAMSCs) isolated from placental tissues have potential for multidifferentiation and immunomodulatory properties and can be easily obtained without the need for invasive procedures and without ethical concerns. This is the first study to use hAMSCs to improve implant osseointegration and bone regeneration after MSFE. Human AMSCs were loaded into a fibrin gel and injected into rabbit MSFE models. The rabbits were assigned to four groups (n=3 per group), i.e., the control group, the hAMSC group, the Bio-Oss group, and the hAMSC/Bio-Oss group. The animals were sacrificed at postsurgery for four and twelve weeks and evaluated by histology and immunohistochemistry. Bone volume, bone volume/tissue volume, bone-to-implant contact ratio, and vessel-like structures in the hAMSC/Bio-Oss group were significantly better than those in other groups in the peri-implant and augmented areas. Immunofluorescence staining showed that alkaline phosphatase (ALP) activities of two hAMSC groups were higher than those of the other two groups. Sequential fluorescent labeling was performed in all of the 12-week groups. Observations showed that hAMSCs accelerated mineralized deposition rates on implant surfaces and in bone-augmented areas. These data demonstrated that hAMSCs could enhance implant osseointegration and bone regeneration after MSFE and might be used to optimize dental implantation in the future.

The Use of Platelet-Rich Plasma Combined With Demineralized Freeze-Dried Bone Allograft in the Treatment of Periodontal Endosseous Defects

2010 ◽  
Vol 141 (8) ◽  
pp. 967-978 ◽  
Author(s):  
Nikolaos Markou ◽  
Eudoxie Pepelassi ◽  
Sotirios Kotsovilis ◽  
Ioannis Vrotsos ◽  
Helen Vavouraki ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Bone Allograft ◽  
Freeze Dried
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