TICK-BORNE ENCEPHALITIS IN KEMEROVO REGION: EPIDEMIOLOGY AND GENETIC DIVERSITY OF THE VIRUS

Efimova A.R.;  ; Karan' L.S.; Drozdova O.M.; Grigorieva Ya.E.; Frolova N.A.; Sheiderova I.D.; Bezrukova E.G.; Kolyasnikova N.M.; Shcherbinina M.S.; Pogodina V.V.;  ;  ;

doi:10.15610/29_1_1

Genetic diversity and geographical distribution of the Siberian subtype of the tick-borne encephalitis virus

Ticks and Tick-borne Diseases ◽

10.1016/j.ttbdis.2019.101327 ◽

2020 ◽

Vol 11 (2) ◽

pp. 101327 ◽

Cited By ~ 8

Author(s):

S.E. Tkachev ◽

I.V. Babkin ◽

G.S. Chicherina ◽

I.V. Kozlova ◽

M.M. Verkhozina ◽

...

Keyword(s):

Genetic Diversity ◽

Geographical Distribution ◽

Encephalitis Virus ◽

Tick Borne Encephalitis ◽

Tick Borne Encephalitis Virus ◽

Siberian Subtype

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Genotyping of tick-borne encephalitis and Kemerovo viruses in taiga ticks collected in the Komi Republic

Russian Journal of Infection and Immunity ◽

10.15789/2220-7619-got-1147 ◽

2020 ◽

Vol 10 (1) ◽

pp. 159-166

Author(s):

M. Yu Kartashov ◽

T. P. Mikryukova ◽

E. I. Krivosheina ◽

A. I. Kuznetsov ◽

L. I. Glushkova ◽

...

Keyword(s):

Genetic Diversity ◽

Komi Republic ◽

Gene Fragment ◽

Tick Bites ◽

The Urals ◽

Tick Borne Encephalitis ◽

Natural Foci ◽

Tick Borne Encephalitis Virus ◽

Far Eastern ◽

Virus Strains

Over the last years, an increasing rate of ixodes tick bites has been registered in the northern regions of the European Russia. In addition, the number of subjects request medical assistance due to tick bites has been dramatically increased in the Komi Republic. In addition, incidence of tick-borne encephalitis was also increased particularly starting since 2009. However, highly limited data on pathogen genetic diversity related to viral tick-borne infections in this region are currently available. Taiga ticks (Ixodespersulcatus) collected from the Komi Republic southern and central part vegetation were examined to identify and genotype tick-borne viruses. Individual ticks were used to identify by RT-PCR viral RNA coupled to tick-borne encephalitis and Kemerovo viruses. Viral genome fragment sequencing allowed to unambiguously identify these viruses. It was found that viral RNA tick-borne encephalitis was detected in 6.8±1.2% individual ticks. Moreover, tick-linked isolate genotyping based on analyzing E protein gene fragment nucleotide sequence derived from tick-borne encephalitis discovered that 35% and 65% isolates belonged to the Far Eastern and Siberian subtype, respectively. In addition, subsequent phylogenetic analysis demonstrated that at least four variants of the Siberian and Far Eastern subtypes of tick-borne encephalitis virus were detected, which were close to the viruses circulating in the Urals and Siberia. In contrast, prevalence of Kemerovo virus in taiga ticks was 0.8±0.2%. Sequencing of Kemerovo virus RNA-dependent RNA polymerase gene fragment showed around 94% homology with the remainder of the Kemerovo virus strains. Phylogenetic analysis of the Kemerovo virus genome fragments demonstrated at least two subtypes circulating in the Komi Republic. Thus, it was suggested that tick-borne encephalitis virus was introduced relatively recently from the Urals and Siberian region into the natural foci of the Komi Republic. Moreover, genetic differences found in Kemerovo virus strains presume for them a longer lasting evolution throughout the natural foci of this region. In addition, a potential role for birds and their ticks in rapid spreading of viral tick-borne infections in the Komi Republic is also discussed. Thus, the data on genetic diversity of the viral agents related to tick-born encephalitis and Kemerovo fever may be useful for improving their diagnostics, prevention and treatment in the Komi Republic.

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Genetic diversity of East European and Asian strains of tick-borne encephalitis virus belonging to Siberian genotype

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2006--24-35 ◽

2006 ◽

Vol 5 ◽

pp. 24-35

Author(s):

L. S. Karan ◽

V. V. Pogodina ◽

T. V. Frolova ◽

A. Ye. Platonov

Keyword(s):

Genetic Diversity ◽

Amino Acid ◽

Amino Acid Position ◽

Encephalitis Virus ◽

The Other ◽

Asian Origin ◽

East European ◽

E Gene ◽

Tick Borne Encephalitis ◽

Tick Borne Encephalitis Virus

We have studied 40 strains of tick-borne encephalitis virus attributed to Siberian genotype. The fragment of E gene (211 nt) has been sequenced for all strains. Futher analysis has evidenced the formation of two clusters. One of them includes the strains of East European (EE) origin, the other comprises the strains of Asian origin (A). 17 of 20 East European strains have histidin (H) in amino acid position 234 of gene E, the remaining 3 have either glutamine (Q) or tyrosine (Y). 13 Asian strains have histidine and 7 Asian strains have glutamine in amino acid position 234.

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