scholarly journals Simultaneous Separation of Active Ingredients Contained in Ginger (Shokyo), Processed Ginger (Kankyo) and Magnolia Bark (Koboku) by Reversed-Phase HPLC with Core-Shell Type Columns: Application to Kampo Products and Food Analysis

2018 ◽  
Vol 39 (3) ◽  
pp. 153-160 ◽  
Author(s):  
Yuriko SUMIDA ◽  
Miho KIMURA ◽  
Tomomi YORIE ◽  
Nanase SOESHIMA ◽  
Kohei KAWABATA ◽  
...  
Keyword(s):  
Food Analysis ◽  
Reversed Phase ◽  
Core Shell ◽  
Reversed Phase Hplc ◽  
Active Ingredients ◽  
Shell Type ◽  
Simultaneous Separation ◽  
Magnolia Bark

scholarly journals The Determination of Six Ionophore Coccidiostats in Feed by Liquid Chromatography with Postcolumn Derivatisation and Spectrofotometric/Fluorescence Detection

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Małgorzata Olejnik ◽  
Piotr Jedziniak ◽  
Teresa Szprengier-Juszkiewicz
Keyword(s):  
Drug Resistance ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Internal Standard ◽  
Reversed Phase ◽  
Feed Additives ◽  
Core Shell ◽  
Proficiency Tests ◽  
Reversed Phase Hplc

The control of levels of anticoccidial feed additives in targeted feeds plays an important role in the assurance of efficiency of animal treatment, prevention of drug resistance, and food safety. The robust and labour-efficient method for the simultaneous determination of six ionophore coccidiostats (lasalocid, maduramicin, monensin, narasin, salinomycin, and semduramicin) in targeted feed has been developed. Properly grinded and homogenized feed sample was spiked with internal standard (monesin methyl ester) and extracted with methanol. The extract was analysed with reversed phase HPLC without any further purification. The separation of the analytes with conventional C18 and core-shell columns was compared. Lasalocid was analysed with fluorescence detection, whereas other ionophores were detected with UV-Vis detector after derivatisation with vanillin in the presence of sulfuric acid. Fortified samples and targeted feeds at authorized levels were used for method validation. Recovery was in the range of 85–110%, depending on the analyte. The within-laboratory reproducibility did not exceed the target value from Horwitz equation. The results of the proficiency tests (z-scores in the range of −1.0 to 1.9) confirmed the reliability of the developed protocol.

scholarly journals A Reversed-phase HPLC Study of the Complexation of Benzene Derivatives Guest Molecules with 5,17-bis(N-Tolyliminomethyl)-25,27-dipropoxycalix[4]arene in Acetonitrile–Water Solution

1999 ◽  
Vol 23 (1) ◽  
pp. 60-61
Author(s):  
O. I. Kalchenko ◽  
A. V. Solovyov ◽  
J. Lipkowski ◽  
V. I. Kalchenko
Keyword(s):  
Stability Constants ◽  
Water Solution ◽  
Reversed Phase ◽  
Benzene Derivatives ◽  
Reversed Phase Hplc ◽  
Guest Molecules ◽  
Hplc Study

Stability constants of the host–guest complexes of 5,17-bis( N-tolyliminomethyl)-25,27-dipropoxycalix[4]arene with benzene derivatives were determined by reversed-phase HPLC in acetonitrile–water solution.

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