scholarly journals Size Exclusion Chromatography Coupled with Multi-Angle Light Scattering Analysis of Physicochemical Properties of Block Copolymer Micelles

2015 ◽  
Vol 36 (1) ◽  
pp. 29-32
Author(s):  
Kumiko SAKAI-KATO ◽  
Kunie NANJO ◽  
Toru KAWANISHI ◽  
Haruhiro OKUDA ◽  
Yukihiro GODA
Keyword(s):  
Light Scattering ◽  
Block Copolymer ◽  
Physicochemical Properties ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Exclusion Chromatography ◽  
Block Copolymer Micelles ◽  
Copolymer Micelles

scholarly journals Comprehensive characterization and quantification of adeno associated vectors by size exclusion chromatography and multi angle light scattering

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicole L. McIntosh ◽  
Geoffrey Y. Berguig ◽  
Omair A. Karim ◽  
Christa L. Cortesio ◽  
Rolando De Angelis ◽  
...  
Keyword(s):  
Light Scattering ◽  
Size Exclusion Chromatography ◽  
Molar Mass ◽  
Size Exclusion ◽  
Minimal Sample ◽  
Accuracy And Precision ◽  
Vector Genome ◽  
Exclusion Chromatography ◽  
Novel Applications

AbstractAdeno associated virus (AAV) capsids are a leading modality for in vivo gene delivery. Complete and precise characterization of capsid particles, including capsid and vector genome concentration, is necessary to safely and efficaciously dose patients. Size exclusion chromatography (SEC) coupled to multiangle light scattering (MALS) offers a straightforward approach to comprehensively characterize AAV capsids. The current study demonstrates that this method provides detailed AAV characterization information, including but not limited to aggregation profile, size-distribution, capsid content, capsid molar mass, encapsidated DNA molar mass, and total capsid and vector genome titer. Currently, multiple techniques are required to generate this information, with varying accuracy and precision. In the current study, a new series of equations for SEC-MALS are used in tandem with intrinsic properties of the capsids and encapsidated DNA to quantify multiple physical AAV attributes in one 20-min run with minimal sample manipulation, high accuracy, and high precision. These novel applications designate this well-established method as a powerful tool for product development and process analytics in future gene therapy programs.

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