Respiratory tract microbiocenosis in children with cystic fibrosis in Kharkiv region

2016 ◽  
Vol 78 (6) ◽  
pp. 24-26
Author(s):  
V.A. Klymenko ◽  
◽  
Y.A. Yanovskaya ◽  
Y.V. Pasichnik ◽  
◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Tract

Preliminary Report of In vitro and In vivo Effectiveness of Dornase Alfa on SARS-CoV-2 Infection (Preprint)

2020 ◽  
Author(s):  
Hacer Kuzu Okur ◽  
Koray Yalcin ◽  
Cihan Tastan ◽  
Sevda Demir ◽  
Bulut Yurtsever ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Tract ◽  
Mononuclear Cells ◽  
Multiple Organ ◽  
Peripheral Blood Mononuclear ◽  
Dornase Alfa ◽  
Tract Infections ◽  
Adult Peripheral Blood

UNSTRUCTURED Dornase alfa, the recombinant form of the human DNase I enzyme, breaks down neutrophil extracellular traps (NET) that include a vast amount of DNA fragments, histones, microbicidal proteins and oxidant enzymes released from necrotic neutrophils in the highly viscous mucus of cystic fibrosis patients. Dornase alfa has been used for decades in patients with cystic fibrosis to reduce the viscoelasticity of respiratory tract secretions, to decrease the severity of respiratory tract infections, and to improve lung function. Previous studies have linked abnormal NET formations to lung diseases, especially to acute respiratory distress syndrome (ARDS). Coronavirus disease 2019 (COVID-19) pandemic affected more than two million people over the world, resulting in unprecedented health, social and economic crises. The COVID-19, viral pneumonia that progresses to ARDS and even multiple organ failure, is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High blood neutrophil levels are an early indicator of SARS-CoV-2 infection and predict severe respiratory diseases. A similar mucus structure is detected in COVID-19 patients due to the accumulation of excessive NET in the lungs. Here, we show our preliminary results with dornase alfa that may have an in-vitro anti-viral effect against SARS-CoV-2 infection in a bovine kidney cell line, MDBK without drug toxicity on healthy adult peripheral blood mononuclear cells. In this preliminary study, we also showed that dornase alfa can promote clearance of NET formation in both an in-vitro and three COVID-19 cases who showed clinical improvement in radiological analysis (2-of-3 cases), oxygen saturation (SpO2), respiratory rate, disappearing of dyspnea and coughing.

scholarly journals Serial Analysis of the Gut and Respiratory Microbiome in Cystic Fibrosis in Infancy: Interaction between Intestinal and Respiratory Tracts and Impact of Nutritional Exposures

mBio ◽  
2012 ◽  
Vol 3 (4) ◽  
Author(s):  
J. C. Madan ◽  
D. C. Koestler ◽  
B. A. Stanton ◽  
L. Davidson ◽  
L. A. Moulton ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Tract ◽  
Microbial Communities ◽  
Effective Means ◽  
Significant Proportion ◽  
Gut Colonization ◽  
Pulmonary Damage ◽  
Chronic Colonization ◽  
High Degree ◽  
Over Time

ABSTRACT Pulmonary damage caused by chronic colonization of the cystic fibrosis (CF) lung by microbial communities is the proximal cause of respiratory failure. While there has been an effort to document the microbiome of the CF lung in pediatric and adult patients, little is known regarding the developing microflora in infants. We examined the respiratory and intestinal microbiota development in infants with CF from birth to 21 months. Distinct genera dominated in the gut compared to those in the respiratory tract, yet some bacteria overlapped, demonstrating a core microbiota dominated by Veillonella and Streptococcus. Bacterial diversity increased significantly over time, with evidence of more rapidly acquired diversity in the respiratory tract. There was a high degree of concordance between the bacteria that were increasing or decreasing over time in both compartments; in particular, a significant proportion (14/16 genera) increasing in the gut were also increasing in the respiratory tract. For 7 genera, gut colonization presages their appearance in the respiratory tract. Clustering analysis of respiratory samples indicated profiles of bacteria associated with breast-feeding, and for gut samples, introduction of solid foods even after adjustment for the time at which the sample was collected. Furthermore, changes in diet also result in altered respiratory microflora, suggesting a link between nutrition and development of microbial communities in the respiratory tract. Our findings suggest that nutritional factors and gut colonization patterns are determinants of the microbial development of respiratory tract microbiota in infants with CF and present opportunities for early intervention in CF with altered dietary or probiotic strategies. IMPORTANCE While efforts have been focused on assessing the microbiome of pediatric and adult cystic fibrosis (CF) patients to understand how chronic colonization by these microbes contributes to pulmonary damage, little is known regarding the earliest development of respiratory and gut microflora in infants with CF. Our findings suggest that colonization of the respiratory tract by microbes is presaged by colonization of the gut and demonstrated a role of nutrition in development of the respiratory microflora. Thus, targeted dietary or probiotic strategies may be an effective means to change the course of the colonization of the CF lung and thereby improve patient outcomes.

Cost of therapy for cystic fibrosis in different age groups with consideration to the respiratory tract infection and complications

2021 ◽  
Vol 31 (2) ◽  
pp. 238-249
Author(s):  
E. G. Furman ◽  
V. V. Shadrina ◽  
T. Yu. Maksimycheva ◽  
V. D. Sherman ◽  
E. I. Kondratyeva
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Tract ◽  
Pharmacoeconomic Analysis ◽  
Age Groups ◽  
Eradication Therapy ◽  
Cost Savings ◽  
Hypothetical Patient ◽  
Severity Of The Disease ◽  
Cost Of Drugs ◽  
The Cost

Despite the low prevalence of cystic fibrosis (CF), the costs of treating this disease are significant for the healthcare system. The aim of this economic study was to assess the total cost of drugs per year for children with CF, depending on age and course of the disease.Methods. For this pharmacoeconomic analysis, groups of “hypothetical” patients were formed according to the characteristics of the course of CF, taking into account the required volume of basic CF therapy, antibiotic therapy, and complications.Results. The cost of basic treatment for CF patients of different ages and disease progression in the hypothetical patient models can vary greatly. The cost of CF therapy increases significantly with age and Pseudomonas aeruginosa respiratory infection, especially in the patients with chronic infection, concomitant polyposis sinusitis, and low nutritional status.Conclusion. Patients with CF require regular follow-up with control of the microflora of the respiratory tract and effective eradication therapy for P. aeruginosa and other pathogenic microorganisms. Reducing infection rates and the severity of the disease can lead to significant cost savings.

Ceftazidime treatment of chronic Pseudomonas aeruginosa respiratory tract infection in cystic fibrosis

1983 ◽  
Vol 12 (suppl A) ◽  
pp. 313-323 ◽  
Author(s):  
H. Permin ◽  
C. Koch ◽  
N. Heiby ◽  
H. O. Christensen ◽  
A. F. Moller ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection

scholarly journals CLINICAL CHARACTERISTICS OF CHILDREN WITH CYSTIC FIBROSIS IN KHARKIV REGION

2017 ◽  
Vol 3 (4) ◽  
pp. 166-169
Author(s):  
Ekateryna Yanovska ◽  
Victoria Klimenko ◽  
Elena Pasichnik
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Tract ◽  
Ct Scan ◽  
Clinical Characteristics ◽  
Epithelial Lining ◽  
Bacteriological Examination ◽  
Epithelial Lining Fluid ◽  
X Ray ◽  
Chest X Ray ◽  
Microbiological Status

The work is devoted to the study of the clinical and paraclinical peculiarities in children with CF (including respiratory tract microbiocenosis) in Kharkiv region. Also it is about the correlation of microbiological status of CF patients with the disease morbidity.Under the supervision were 30 children with cystic fibrosis. They conducted clinical, paraclinical (bacteriological examination of sputum and epithelial lining fluid, chest X-ray, CT scan of lungs) were performed.Clinical and paraclinic (bacteriological examination of sputum and epithelial lining fluid, chest X-ray, CT scan of lungs) were performed.During observations revealed that the condition severity of CF patients is associated with chronic P. aeruginosa infection, and B. cepacia. And also, that none of patients in Kharkiv region has determined  any of pathognomonic respiratory causative microorganisms – M. Tuberculosis and non-tuberculous micobacteria, H. influenza, Ralstonia picketi, condition of infecting by P. Aeruginosa is not identified, and this is the evidence of insufficient laboratory diagnostics.Key words: cystiс fibrosis, children, microflora В.А. Клименко, К.О. Яновська, О.В. ПасічникКЛІНІЧНА ХАРАКТЕРИСТИКА ДІТЕЙ З МУКОВІСЦИДОЗОМ В ХАРКІВСЬКОМУ РЕГІОНІРобота присвячена вивченню клініко-параклінічних особливостей дітей з МВ (в тому числі - мікробіоценоз респіраторного тракту) в Харківському регіоні. А також визначенню кореляції мікробіологічного статусу хворих МВ з тяжкістю перебігу захворювання.Під наглядом були 30 дітей, хворих на муковісцидоз. Їм проведені клінічні, параклінічні (бактеріологічне дослідження мокротиння і промивних вод бронхів, рентгенограма органів грудної клітки, комп'ютерна томографія легенів) дослідження.В ході спостережень виявлено, що тяжкість стану хворих МВ асоційована з хронічною інфекцією P. aeruginosa і B. cepacia. А також, що в Харківському регіоні з патогномонічних респіраторних збудників МВ у жодного хворого не виявлено M. Tuberculosis і non-tuberculous micobacteria, H. influenza, Ralstonia picketi, не визначається статус інфікування P. aeruginosa, що свідчить про незадовільну лабораторну діагностику.Ключові слова: муковісцидоз, діти, мікрофлора. В.А.  Клименко, Е.А. Яновская, Е.В. ПасичникКЛИНИЧЕСКАЯ ХАРАКТЕРИСТИКА ДЕТЕЙ С МУКОВИСЦИДОЗОМ В ХАРЬКОВСКОМ РЕГИОНЕРабота посвящена изучению клинико-параклинических особенностей детей с МВ (в том числе – микробиоценоз респираторного тракта) в Харьковском регионе. А также определению корреляции микробиологического статуса больных МВ с тяжестью течения заболевания.Под наблюдением были 30 детей, больных муковисцидозом. Им проведены клинические, параклинические (бактериологическое исследование мокроты и промывных вод бронхов, рентгенограмма органов грудной клетки, компьютерная томография легких) исследования.В ходе наблюдений выявлено, что тяжесть состояния больных МВ ассоциирована с хронической инфекцией P. aeruginosa и B. cepacia.  А также, что в Харьковском регионе из патогномоничных респираторных возбудителей МВ ни у одного больного не выявлено M. Tuberculosis и non-tuberculous micobacteria, H. influenza, Ralstonia picketi, не определяется статус инфицирования P. aeruginosa, что свидетельствует о неудовлетворительной лабораторной диагностике.Ключевые слова: муковисцидоз, дети,  микрофлора.

Phenazine Content in the Cystic Fibrosis Respiratory Tract Negatively Correlates with Lung Function and Microbial Complexity

2012 ◽  
Vol 47 (6) ◽  
pp. 738-745 ◽  
Author(s):  
Ryan C. Hunter ◽  
Vanja Klepac-Ceraj ◽  
Magen M. Lorenzi ◽  
Hannah Grotzinger ◽  
Thomas R. Martin ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Lung Function ◽  
Respiratory Tract

Recovery of Pseudomonas gladioli from respiratory tract specimens of patients with cystic fibrosis.

1989 ◽  
Vol 27 (2) ◽  
pp. 270-273 ◽  
Author(s):  
J C Christenson ◽  
D F Welch ◽  
G Mukwaya ◽  
M J Muszynski ◽  
R E Weaver ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Tract
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