Attenuation- and Phase-Contrast Microtomography Using Synchrotron Radiation for the 3-D Investigation of Specimens Consisting of Elements With Low and Medium Absorption

1999 ◽  
Vol 590 ◽  
Author(s):  
F. Beckmann ◽  
U. Bonse
Keyword(s):  
Synchrotron Radiation ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
High Spatial Resolution ◽  
X Ray ◽  
Contrast Mechanism

ABSTRACTAttenuation- and phase-contrast microtomography using synchrotron radiation is applied to different samples demonstrating the advantages and limits of the two different contrast mechanism. Photon energies in the range of 8-25 keV and 60-100 keV are used. Scanning techniques employing a 2-dim. x-ray detector allow for investigation of larger specimens at high spatial resolution.

Effect of Coating with Hydroxyapatite on the Bonding of Bone to Implant

2012 ◽  
Vol 706-709 ◽  
pp. 1661-1666
Author(s):  
Abdelilah Benmarouane ◽  
Pierre Millet ◽  
Thomas Buslaps ◽  
Alain Lodini ◽  
Veijo Honkimäki
Keyword(s):  
Synchrotron Radiation ◽  
Spatial Resolution ◽  
Orthopaedic Surgery ◽  
High Spatial Resolution ◽  
High Energy ◽  
Direct Connection ◽  
X Ray Diffraction ◽  
X Ray ◽  
Natural Bone ◽  
Non Destructive

The aim of the present study was to study the interface implant-bone by synchrotron radiation, the implant has two faces the first one coated with hydroxyapatite and the second uncoated. In orthopaedic surgery, Titanium (Ti-Al-4V) implants are currently coated with hydroxyapatite (HAp), Ca10(PO4)6(OH)2, in order to obtain a stable and functional direct connection between the bone and the implant. At the implant-bone interface, the new bone reconstituted after two months of implantation must have the same properties like the natural bone in order to accept the implant. Therefore we studied the texture of the reconstituted bone crystals at the interface applying non destructive x-ray diffraction. The required high spatial resolution was achieved utilizing high-energy synchrotron radiation on ID15 at ESRF in Grenoble, France.

scholarly journals X-ray phase-contrast tomography for high-spatial-resolution zebrafish muscle imaging

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
William Vågberg ◽  
Daniel H. Larsson ◽  
Mei Li ◽  
Anders Arner ◽  
Hans M. Hertz
Keyword(s):  
Spatial Resolution ◽  
Phase Contrast ◽  
High Spatial Resolution ◽  
X Ray ◽  
Phase Contrast Tomography

scholarly journals High-resolution tomographic imaging of a human cerebellum: comparison of absorption and grating-based phase contrast

2010 ◽  
Vol 7 (53) ◽  
pp. 1665-1676 ◽  
Author(s):  
Georg Schulz ◽  
Timm Weitkamp ◽  
Irene Zanette ◽  
Franz Pfeiffer ◽  
Felix Beckmann ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Brain Tissue ◽  
Purkinje Cells ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Stratum Granulosum ◽  
X Ray ◽  
Human Cerebellum ◽  
Absorption Contrast

Human brain tissue belongs to the most impressive and delicate three-dimensional structures in nature. Its outstanding functional importance in the organism implies a strong need for brain imaging modalities. Although magnetic resonance imaging provides deep insights, its spatial resolution is insufficient to study the structure on the level of individual cells. Therefore, our knowledge of brain microstructure currently relies on two-dimensional techniques, optical and electron microscopy, which generally require severe preparation procedures including sectioning and staining. X-ray absorption microtomography yields the necessary spatial resolution, but since the composition of the different types of brain tissue is similar, the images show only marginal contrast. An alternative to absorption could be X-ray phase contrast, which is known for much better discrimination of soft tissues but requires more intricate machinery. In the present communication, we report an evaluation of the recently developed X-ray grating interferometry technique, applied to obtain phase-contrast as well as absorption-contrast synchrotron radiation-based microtomography of human cerebellum. The results are quantitatively compared with synchrotron radiation-based microtomography in optimized absorption-contrast mode. It is demonstrated that grating interferometry allows identifying besides the blood vessels, the stratum moleculare, the stratum granulosum and the white matter. Along the periphery of the stratum granulosum, we have detected microstructures about 40 µm in diameter, which we associate with the Purkinje cells because of their location, size, shape and density. The detection of individual Purkinje cells without the application of any stain or contrast agent is unique in the field of computed tomography and sets new standards in non-destructive three-dimensional imaging.

High-Spatial-Resolution Three-dimensional Imaging of Human Spinal Cord and Column Anatomy with Postmortem X-ray Phase-Contrast Micro-CT

Radiology ◽  
2021 ◽  
Vol 298 (1) ◽  
pp. 135-146
Author(s):  
Giacomo E. Barbone ◽  
Alberto Bravin ◽  
Alberto Mittone ◽  
Sergio Grosu ◽  
Jens Ricke ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
High Spatial Resolution ◽  
Three Dimensional ◽  
Micro Ct ◽  
Three Dimensional Imaging ◽  
X Ray ◽  
Human Spinal Cord

scholarly journals High-Spatial-Resolution Bone Densitometry with Dual-Energy X-ray Absorptiometric Region-free Analysis

Radiology ◽  
2015 ◽  
Vol 275 (1) ◽  
pp. 310-310 ◽  
Author(s):  
Richard M. Morris ◽  
Lang Yang ◽  
Miguel A. Martín-Fernández ◽  
Jose M. Pozo ◽  
Alejandro F. Frangi ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Bone Densitometry ◽  
High Spatial Resolution ◽  
Dual Energy ◽  
X Ray

scholarly journals Visualization Ability of Phase-Contrast Synchrotron-Based X-Ray Imaging Using an X-Ray Interferometer in Soft Tissue Tumors

2021 ◽  
Vol 20 ◽  
pp. 153303382110101
Author(s):  
Thet-Thet Lwin ◽  
Akio Yoneyama ◽  
Hiroko Maruyama ◽  
Tohoru Takeda
Keyword(s):  
Computed Tomography ◽  
Soft Tissue ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
Soft Tissue Tumors ◽  
X Ray ◽  
3 Dimensional ◽  
Computed Tomography Images ◽  
X Ray Imaging ◽  
X Ray Computed

Phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer provides high sensitivity and high spatial resolution, and it has the ability to depict the fine morphological structures of biological soft tissues, including tumors. In this study, we quantitatively compared phase-contrast synchrotron-based X-ray computed tomography images and images of histopathological hematoxylin-eosin-stained sections of spontaneously occurring rat testicular tumors that contained different types of cells. The absolute densities measured on the phase-contrast synchrotron-based X-ray computed tomography images correlated well with the densities of the nuclear chromatin in the histological images, thereby demonstrating the ability of phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer to reliably identify the characteristics of cancer cells within solid soft tissue tumors. In addition, 3-dimensional synchrotron-based phase-contrast X-ray computed tomography enables screening for different structures within tumors, such as solid, cystic, and fibrous tissues, and blood clots, from any direction and with a spatial resolution down to 26 μm. Thus, phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer shows potential for being useful in preclinical cancer research by providing the ability to depict the characteristics of tumor cells and by offering 3-dimensional information capabilities.

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