Small-Angle Neutron Scattering of Bilayer Vesicles Made with Synthetic Phospholipids

1998 ◽  
Vol 550 ◽  
Author(s):  
S. F. Trevino ◽  
Robert lvkov ◽  
Gary R. Matyas ◽  
Frank J. Lebeda
Keyword(s):  
Neutron Scattering ◽  
Lipid Bilayers ◽  
Small Angle ◽  
Lipid Membranes ◽  
Small Angle Neutron Scattering ◽  
Bilayer Lipid Membranes ◽  
Lipid Concentration ◽  
The Core ◽  
Delivery Vehicles ◽  
Therapeutic Compounds

AbstractWe have used Small-Angle Neutron Scattering to investigate the structure of bilayer lipid membranes in aqueous solution. The lipids consist of equimolar concentrations of two molecules DMPG and DMPC (see text). The structures consist of solvent filled cores surrounded by shells composed of the lipid bilayers. In particular the radii of the core and shell thickness are measured as a function of lipid concentration and temperature. Other features which reveal themselves are vesicle forming ability of the lipids, additional larger structures and inter-vesicle interactions at large vesicle concentrations. The study is motivated by the possible use of these systems as delivery vehicles for various beneficial therapeutic compounds.

Comment on “Cholesterol solubility limit in lipid membranes probed by small angle neutron scattering and MD simulations” by S. Garg et al., Soft Matter, 2014, 10, 9313

Soft Matter ◽  
2015 ◽  
Vol 11 (27) ◽  
pp. 5580-5581
Author(s):  
Richard M. Epand ◽  
Diana Bach ◽  
Ellen Wachtel
Keyword(s):  
Phase Separation ◽  
Neutron Scattering ◽  
Small Angle ◽  
Lipid Membranes ◽  
Small Angle Neutron Scattering ◽  
Soft Matter ◽  
Md Simulations ◽  
Solubility Limit ◽  
Phospholipid Bilayers ◽  
Cholesterol Solubility

As consistently described in the literature, the solubility limit of cholesterol in phospholipid bilayers is defined by its phase separation and crystallization.

Cholesterol solubility limit in lipid membranes probed by small angle neutron scattering and MD simulations

Soft Matter ◽  
2014 ◽  
Vol 10 (46) ◽  
pp. 9313-9317 ◽  
Author(s):  
Sumit Garg ◽  
Francisco Castro-Roman ◽  
Lionel Porcar ◽  
Paul Butler ◽  
Pedro Jesus Bautista ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Neutron Scattering ◽  
Small Angle ◽  
Lipid Membranes ◽  
Small Angle Neutron Scattering ◽  
Md Simulations ◽  
Solubility Limit ◽  
Coarse Grained ◽  
Small Unilamellar Vesicles ◽  
Cholesterol Solubility

The solubility limits of cholesterol in small unilamellar vesicles made of POPS and POPC were probed using Small Angle Neutron Scattering (SANS) and coarse grained (CG) molecular dynamics (MD) simulations.

Small-angle neutron scattering method for in situ studies of the dense cores of biological cells and vesicles: application to isolated neurosecretory vesicles

1989 ◽  
Vol 22 (6) ◽  
pp. 546-555 ◽  
Author(s):  
S. Krueger ◽  
J. W. Lynn ◽  
J. T. Russell ◽  
R. Nossal
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Scattering Method ◽  
Contrast Variation ◽  
Model Calculations ◽  
The Core ◽  
Dense Cores ◽  
Dense State

Small-angle neutron scattering was used to study the structure of the dense cores of intact neurosecretory vesicles (NSV). Contrast-variation techniques were used to minimize the scattering due to vesicle membranes and emphasize that due to the cores. By examination of a suspension of NSV membranes along with the intact NSV, residual membrane scattering was suppressed. The resultant scattering is inconsistent with model calculations which assume that the dense state of the vesicle cores is achieved by random dense packing or crystallization of small globular particles. Rather, the data suggest that the core constituents exist in a disordered state, forming aggregates with radii of gyration significantly larger than 100 Å.

scholarly journals Nanoscale Structure of Lipid Bilayers Revealed By In-Silico and Experimental Small Angle Neutron Scattering

2018 ◽  
Vol 114 (3) ◽  
pp. 269a
Author(s):  
Mitchell Dorrell ◽  
Fred Heberle ◽  
John Katsaras ◽  
Ed Lyman ◽  
Alexander Sodt
Keyword(s):  
Neutron Scattering ◽  
Lipid Bilayers ◽  
Small Angle ◽  
In Silico ◽  
Small Angle Neutron Scattering ◽  
Nanoscale Structure

scholarly journals The microstructure and morphology of carbon black: A study using small angle neutron scattering and contrast variation

1994 ◽  
Vol 9 (12) ◽  
pp. 3210-3222 ◽  
Author(s):  
Rex P. Hjelm ◽  
Wesley A. Wampler ◽  
Philip A. Seeger ◽  
Michel Gerspacher
Keyword(s):  
Carbon Black ◽  
Neutron Scattering ◽  
Internal Structure ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
High Surface ◽  
High Surface Area ◽  
Major Axis ◽  
Contrast Variation ◽  
The Core

This is a study of the form and structure of particles and particle aggregates of an experimental high surface area carbon black (HSA) using small-angle neutron scattering and the method of contrast variation. Contrast variation was effected by studying suspensions of the carbon black in cyclohexane containing different fractions of deuterocyclohexane. We find that the approximately 29 nm diameter USA particles are arranged as small, linear aggregates with an average aggregation number between 4 and 6. The structure averaged over the particle population is best represented by a prolate ellipsoid of revolution with semiaxes 14.5 and 76.4 nm. The surface of the aggregates appears smooth over length scales larger than 1 nm, which places an upper limit on the surface roughness observed by other methods. The internal structure of the aggregates is described by a shell-core model, with the shell density being consistent with a graphitic structure and the core being of lower density, more like amorphous carbon. Some fraction of the core volume (0.1 to 0.2) is taken up by voids that are not accessible to the solvent. An estimate of the shell thickness gives 1 to 2 nm along the ellipsoid minor axis and 6 to 10 nm along the major axis. The particles of the aggregate appear to be fused so that the less dense amorphous core is continuous through the inner parts of the aggregate. The information that can be obtained on the internal structure using contrast variation is limited by heterogeneity in the chemical composition of carbon black aggregates.

scholarly journals Membrane Protein Structures in Lipid Bilayers; Small-Angle Neutron Scattering With Contrast-Matched Bicontinuous Cubic Phases

2021 ◽  
Vol 8 ◽  
Author(s):  
Charlotte E. Conn ◽  
Liliana de Campo ◽  
Andrew E. Whitten ◽  
Christopher J. Garvey ◽  
Anwen M. Krause-Heuer ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Neutron Scattering ◽  
Phase Behavior ◽  
Lipid Bilayers ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Protein Structures ◽  
Bicontinuous Cubic ◽  
Contrast Matching ◽  
Detergent Micelles

This perspective describes advances in determining membrane protein structures in lipid bilayers using small-angle neutron scattering (SANS). Differentially labeled detergents with a homogeneous scattering length density facilitate contrast matching of detergent micelles; this has previously been used successfully to obtain the structures of membrane proteins. However, detergent micelles do not mimic the lipid bilayer environment of the cell membrane in vivo. Deuterated vesicles can be used to obtain the radius of gyration of membrane proteins, but protein-protein interference effects within the vesicles severely limits this method such that the protein structure cannot be modeled. We show herein that different membrane protein conformations can be distinguished within the lipid bilayer of the bicontinuous cubic phase using contrast-matching. Time-resolved studies performed using SANS illustrate the complex phase behavior in lyotropic liquid crystalline systems and emphasize the importance of this development. We believe that studying membrane protein structures and phase behavior in contrast-matched lipid bilayers will advance both biological and pharmaceutical applications of membrane-associated proteins, biosensors and food science.

scholarly journals Probing the Complex Loading Dependent Structural Changes in Ultra-High Drug Loaded Polymer Micelles by Small-Angle Neutron Scattering

2019 ◽  
Author(s):  
Benedikt Sochor ◽  
Özgür Düdückcü ◽  
Michael M Lübtow ◽  
Bernhard Schummer ◽  
Sebastian Jaksch ◽  
...  
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Drug Loading ◽  
Scattering Data ◽  
Resonance Spectroscopy ◽  
Polymer Micelle ◽  
Polymer Micelles ◽  
The Core ◽  
The Impact

Drug loaded polymer micelles or nanoparticles are being continuously explored in the fields of drug delivery and nanomedicine. Commonly, a simple core-shell structure is assumed, in which the core incorporates the drug and the corona provides steric shielding, colloidal stability, and prevents protein adsorption. Recently, the interactions of the dissolved drug with the micellar corona have received increasing attention. Here, using small-angle neutron scattering, we provide an in-depth study of the differences in polymer micelle morphology of a small selection of structurally closely related polymer micelles at different loadings with the model compound curcumin. This work supports a previous study using solid state nuclear magnetic resonance spectroscopy and we confirm that the drug resides predominantly in the core of the micelle at low drug loading. As the drug loading increases, neutron scattering data suggests that an inner shell is formed, which we interpret as the corona also starting to incorporate the drug, whereas the outer shell mainly contains water and the polymer. The presented data clearly shows that a better understanding of the inner morphology and the impact of the hydrophilic block can be important parameters for improved drug loading in polymer micelles as well as provide insights into structure-property relationships.<br>

scholarly journals Nanoscale Structure of Lipid Bilayers Revealed by In-Silico and Experimental Small Angle Neutron Scattering

2019 ◽  
Vol 116 (3) ◽  
pp. 90a
Author(s):  
Mitchell Dorrell ◽  
Frederick A. Heberle ◽  
John Katsaras ◽  
Edward Lyman ◽  
Alexander J. Sodt
Keyword(s):  
Neutron Scattering ◽  
Lipid Bilayers ◽  
Small Angle ◽  
In Silico ◽  
Small Angle Neutron Scattering ◽  
Nanoscale Structure
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