scholarly journals An in Situ High Voltage Electron Microscopy Technique for the Study of Deformation and Fracture: in Multilayered Materials

1995 ◽  
Vol 382 ◽  
Author(s):  
M.A. Wall ◽  
T.W. Barbee ◽  
T.P. Weihs
Keyword(s):  
High Voltage ◽  
Tensile Deformation ◽  
Specimen Preparation ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
Microscopy Technique ◽  
High Voltage Electron ◽  
Deformation And Fracture ◽  
Multilayered Materials

ABSTRACTA novel, in situ, high voltage electron microcopy technique for the direct observation of the micromechanisms of tensile deformation and fracture in nanostructured materials is detailed. This technique is particularly well suited for the dynamic observations of deformation and fracture in multilayered materials. The success of this type of in situ technique is highly dependent upon unique specimen preparation procedures and sample design, the importance thereof will be discussed. The initial observations discussed here are expected to aid in our understanding of the mechanical behavior of this new class of atomically engineered materials.

On the High Voltage Electron Microscopy (1 MeV) of Biological Thick Sections

1972 ◽  
Vol 30 ◽  
pp. 464-465
Author(s):  
William H. Massover
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Present Report ◽  
Biological Tissues ◽  
Specimen Preparation ◽  
Technical Problems ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
High Voltage Electron

Stereoscopic examination of thick sections of fixed and embedded biological tissues by high voltage electron microscopy has been shown to allow direct visualization of three-dimensional fine structure. The present report will consider the occurrence of some new technical problems in specimen preparation and Image interpretation that are not common during lower voltage studies of thin sections.Thick Sectioning and Tissue Coloration - Epon sections of 0.5 μm or more that are cut with glass knives do not have a uniform thickness as Judged by their interference colors; these colors change with time during their flotation on the knife bath, and again when drying onto the specimen support. Quoted thicknesses thus must be considered only as rough estimates unless measured in specific regions by other methods. Chloroform vapors do not always result in good spreading of thick sections; however, they will spread spontaneously to large degrees after resting on the flotation bath for several minutes. Ribbons of thick sections have been almost impossible to obtain.

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