Hydroxyapatite Coatings of Varying Crystallinity

1993 ◽  
Vol 331 ◽  
Author(s):  
Suzanne H. Maxian ◽  
Janice B. Liesch ◽  
Moti L. Tiku ◽  
Joseph P. Zawadsky ◽  
Michael G. Dunn
Keyword(s):  
Tissue Culture ◽  
Alkaline Phosphatase ◽  
Cell Attachment ◽  
Fibroblast Cell ◽  
Bone Cell ◽  
Titanium Implants ◽  
Interface Failure ◽  
Hydroxyapatite Coatings ◽  
Coated Surfaces

AbstractQuestions remain as to the integrity of hydroxyapatite (HA) coatings on orthopaedic implants and the possibility of failure at the coating/implant interface in vivo. Previous investigations of resorbable HA coated implants showed bone bonding strengths equal to non-resorbable HA coated surfaces in interference fit surgical model. If the concept of a resorbing coating will alleviate concerns of interface failure, further investigation as to the mode of coating resorption and the cellular events at the interface are required. The effect of coating these implants with Matrigel, as a means to enhance bone cell attachment and growth was studied. Fibroblast cell behavior on resorbable, non-resorbable HA coated and uncoated rough titanium implants were studied at 4 and 24 hours for attachment and spreading. Postnatal rat calvarial cell attachment, growth and morphology studies were also performed on these three implants and on tissue culture plates with and without Matrigel at 2, 7, and 10 days. Resorbable HA coatings showed greater surface activity and cell spreading, but not greater cell attachment at 4 and 24 hours. Greater alkaline phosphatase production per cell was seen on Matrigel coated tissue culture plates than uncoated wells. Cytoplasmic processes similar to osteocytic canalicular networks were seen. Matrigel coating on the three types of implants showed slightly greater alkaline phosphatase production of cells than without Matrigel coating.

scholarly journals Simultaneous autoradiographic and histochemical analysis of bone formed in vitro.

1986 ◽  
Vol 34 (6) ◽  
pp. 769-773 ◽  
Author(s):  
H C Tenenbaum ◽  
C A McCulloch ◽  
K Palangio
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Time Course ◽  
Bone Cell ◽  
Histochemical Demonstration ◽  
Thymidine Uptake ◽  
Kinetics In Vivo

The simultaneous histochemical demonstration of alkaline phosphatase activity and autoradiographic demonstration of [3H]-thymidine uptake is valuable for study of bone cell kinetics in vivo or in vitro. By use of this technique, it has been possible to detect changes induced by a single dose of dexamethasone (10(-7) M) in the time course of alkaline phosphatase activity, the number of alkaline phosphatase-positive cells, and [3H]-thymidine labeling in bone formed in vitro.

The effects of parathyroid hormone on osteoblast-like cells from embryonic chick calvaria

1985 ◽  
Vol 108 (2) ◽  
pp. 217-223 ◽  
Author(s):  
A. K. Hall ◽  
I. R. Dickson
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Bone Cells ◽  
Primary Cultures ◽  
Enzymatic Digestion ◽  
Bone Cell ◽  
Prostaglandin E ◽  
Embryonic Chick ◽  
Low Concentrations

Abstract. A bone cell fraction was isolated from 16 day old embryonic chick calvaria using a sequential enzymatic digestion procedure. The fraction contained cells, of an osteoblast-like character, which responded to parathyroid hormone (PTH) and prostaglandin E2, but not to calcitonin, in terms of increased production of cyclic AMP. Primary cultures of cells maintained their responsiveness to PTH for at least 2 weeks after reaching confluence. Production of alkaline phosphatase by the bone cells was inhibited when 1,25(OH)2 vitamin D3 was added to cultures at concentrations of 10−8 m or greater. When cells were cultured in the presence of PTH a biphasic effect was observed; alkaline phosphatase levels were stimulated at low concentrations of this hormone but were decreased at higher concentrations. The latter finding appears consistent with observations that PTH can in vivo exert either anabolic or catabolic effects on bone, depending upon the circulating level of hormone present.

scholarly journals Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Martina Schleicher ◽  
Jan Hansmann ◽  
Bentsian Elkin ◽  
Petra J. Kluger ◽  
Simone Liebscher ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Human Blood ◽  
Cell Attachment ◽  
Culture Conditions ◽  
Dna Oligonucleotides ◽  
Para Xylene ◽  
Coated Surfaces ◽  
Blood Vessel Substitutes ◽  
Cardiovascular Implants

In vivoself-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the creation of cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces. DNA-oligonucleotides immobilized by adsorption on parylene (poly(monoaminomethyl-para-xylene)) coated polystyrene and ePTFE were resistant to high shear stress (9.5 N/m2) and human blood serum for up to 96 h. Adhesion of murine endothelial progenitor cells, HUVECs and endothelial cells from human adult saphenous veins as well as viability over a period of 14 days of HUVECs on oligonucleotide coated samples under dynamic culture conditions was significantly enhanced (P<0.05). Oligonucleotide-coated surfaces revealed low thrombogenicity and excellent hemocompatibility after incubation with human blood. These properties suggest the suitability of immobilization of DNA-oligonucleotides for biofunctionalization of blood vessel substitutes for improvedin vivoendothelialization.

scholarly journals Comparison of Fibroblast and Osteoblast Response to Cultivation on Titanium Implants with Different Grain Sizes

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Vaclav Babuska ◽  
Jana Dobra ◽  
Vlastimil Kulda ◽  
Michaela Kripnerova ◽  
Amin Moztarzadeh ◽  
...  
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Longitudinal Section ◽  
Titanium Implants ◽  
Grain Sizes ◽  
Osteoblast Cell Line ◽  
Human Fibroblast Cell ◽  
Cellular Behaviour

Thein vitroresponse of human fibroblast cell line HFL1 and human osteoblast cell line hFOB 1.19 on nanostructured titanium with different grain sizes has been compared in the present study. Used samples of titanium produced by equal channel angular (ECA) pressing have grain sizes of 160 nm, 280 nm, and 2400 nm with cross- and longitudinal sections. Similar cellular behaviour was observed on all studied biomaterials. There were significant differences related to the initial phase of attachment, but not in proliferation. Furthermore, the results indicate that osteoblasts grow best on material with grain size of 160 nm with a longitudinal section in comparison with other examined materials. Therefore, this material could be recommended for further evaluation with respect to osseointegrationin vivo.

In vivo behaviour of hydroxyapatite coatings on titanium implants: a quantitative study in the rabbit

Biomaterials ◽  
2002 ◽  
Vol 23 (12) ◽  
pp. 2569-2575 ◽  
Author(s):  
G.L. Darimont ◽  
R. Cloots ◽  
E. Heinen ◽  
L. Seidel ◽  
R. Legrand
Keyword(s):  
Quantitative Study ◽  
Titanium Implants ◽  
Hydroxyapatite Coatings

Osseointegration interface of calcified bone and endosseous implants

1992 ◽  
Vol 50 (2) ◽  
pp. 1096-1097
Author(s):  
K.E. Krizan ◽  
J.E. Laffoon ◽  
M.J. Buckley
Keyword(s):  
Structure And Function ◽  
Titanium Implants ◽  
En Bloc ◽  
Endosseous Implants ◽  
Ultrastructural Studies ◽  
The Past ◽  
Cacodylate Buffer ◽  
One Year ◽  
And Function

With increase use of tissue-integrated prostheses in recent years it is a goal to understand what is happening at the interface between haversion bone and bulk metal. This study uses electron microscopy (EM) techniques to establish parameters for osseointegration (structure and function between bone and nonload-carrying implants) in an animal model. In the past the interface has been evaluated extensively with light microscopy methods. Today researchers are using the EM for ultrastructural studies of the bone tissue and implant responses to an in vivo environment. Under general anesthesia nine adult mongrel dogs received three Brånemark (Nobelpharma) 3.75 × 7 mm titanium implants surgical placed in their left zygomatic arch. After a one year healing period the animals were injected with a routine bone marker (oxytetracycline), euthanized and perfused via aortic cannulation with 3% glutaraldehyde in 0.1M cacodylate buffer pH 7.2. Implants were retrieved en bloc, harvest radiographs made (Fig. 1), and routinely embedded in plastic. Tissue and implants were cut into 300 micron thick wafers, longitudinally to the implant with an Isomet saw and diamond wafering blade [Beuhler] until the center of the implant was reached.

scholarly journals Transformation-associated alterations in interactions between pre-B cells and fibronectin

Blood ◽  
1990 ◽  
Vol 76 (11) ◽  
pp. 2311-2320 ◽  
Author(s):  
FM Lemoine ◽  
S Dedhar ◽  
GM Lima ◽  
CJ Eaves
Keyword(s):  
B Cells ◽  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Cell Attachment ◽  
Attachment Site ◽  
Receptor Antibodies

Abstract Marrow stromal elements produce as yet uncharacterized soluble growth factors that can stimulate the proliferation of murine pre-B cells, although close contact between these two cell types appears to ensure a better pre-B cell response. We have now shown that freshly isolated normal pre-B cells (ie, the B220+, surface mu- fraction of adult mouse bone marrow) adhere to fibronectin (FN) via an RGD cell-attachment site, as shown in a serum-free adherence assay, and they lose this functional ability on differentiation in vivo into B cells (ie, the B220+, surface mu+ fraction). Similarly, cells from an immortalized but stromal cell-dependent and nontumorigenic murine pre-B cell line originally derived from a Whitlock-Witte culture were also found to adhere to fibronectin (FN) via an RGD cell-attachment site. Moreover, in the presence of anti-FN receptor antibodies, the ability of this immortalized pre-B cell line to proliferate when co-cultured with a supportive stromal cell line (M2–10B4 cells) was markedly reduced (down to 30% of control). This suggests that pre-B cell attachment to FN on stromal cells may be an important component of the mechanism by which stromal cells stimulate normal pre-B cell proliferation and one that is no longer operative to control their more differentiated progeny. Two differently transformed pre-B cell lines, both of which are autocrine, stromal-independent, tumorigenic in vivo, and partially or completely differentiation-arrested at a very early stage of pre-B cell development, did not bind to FN. In addition, anti-FN receptor antibodies were much less effective in diminishing the ability of these tumorigenic pre-B cells to respond to M2–10B4 cell stimulation, which could still be demonstrated when the tumorigenic pre-B cells were co- cultured with M2–10B4 cells at a sufficiently low cell density. Analysis of cell surface molecules immunoprecipitated from both the nontumorigenic and tumorigenic pre-B cell lines by an anti-FN receptor antibody showed an increase in very late antigen (VLA) alpha chain(s) in both tumorigenic pre-B cell lines and a decrease in the beta 1 chain in one. Interestingly, all of the pre-B cell lines expressed similar amounts of messenger RNA for the beta 1 chain of the FN receptor. These results suggest that alteration of FN receptor expression on pre-B cells may represent a mechanism contributing to the outgrowth of leukemic pre-B cells with an autocrine phenotype and capable of stromal cell-independent, autonomous growth.

scholarly journals Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts

Coatings ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 781 ◽  
Author(s):  
Paula E. Florian ◽  
Liviu Duta ◽  
Valentina Grumezescu ◽  
Gianina Popescu-Pelin ◽  
Andrei C. Popescu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Doping Agent ◽  
Hydroxyapatite Coatings ◽  
3D Network ◽  
Immunofluorescence Labelling ◽  
Good Biocompatibility ◽  
Adhesion Process

This study is focused on the adhesion and differentiation of the human primary mesenchymal stem cells (hMSC) to osteoblasts lineage on biological-derived hydroxyapatite (BHA) and lithium-doped BHA (BHA:LiP) coatings synthesized by Pulsed Laser Deposition. An optimum adhesion of the cells on the surface of BHA:LiP coatings compared to control (uncoated Ti) was demonstrated using immunofluorescence labelling of actin and vinculin, two proteins involved in the initiation of the cell adhesion process. BHA:LiP coatings were also found to favor the differentiation of the hMSC towards an osteoblastic phenotype in the presence of osteoinductive medium, as revealed by the evaluation of osteoblast-specific markers, osteocalcin and alkaline phosphatase. Numerous nodules of mineralization secreted from osteoblast cells grown on the surface of BHA:LiP coatings and a 3D network-like organization of cells interconnected into the extracellular matrix were evidenced. These findings highlight the good biocompatibility of the BHA coatings and demonstrate that the use of lithium as a doping agent results in an enhanced osteointegration potential of the synthesized biomaterials, which might therefore represent viable candidates for future in vivo applications.

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