Osteoblast Adhesion on Biodegradable Polymer Substrates

1993 ◽  
Vol 331 ◽  
Author(s):  
Susan L. Ishaug ◽  
Michael J. Yaszemski ◽  
Rena Bizios ◽  
Antonios G. Mikos
Keyword(s):  
Tissue Culture ◽  
Alkaline Phosphatase ◽  
Collagen Synthesis ◽  
Glycolic Acid ◽  
Polymer Substrates ◽  
Tissue Culture Polystyrene ◽  
Biodegradable Poly ◽  
Osteoblast Adhesion ◽  
Rat Osteoblasts

AbstractWe have investigated the adhesion of rat osteoblasts on biodegradable poly(α-hydroxy ester) films as an in vitro model of bone regeneration. Osteoblasts cultured on poly(L-lactic acid), poly(DL-lactic-co-glycolic acid) copolymers, and poly(glycolic acid) films for 14 days grew with rates comparable to those observed for tissue culture polystyrene and retained their phenotype as expressed by activity of alkaline phosphatase and collagen synthesis.

Acidosis inhibits osteoblastic and stimulates osteoclastic activity in vitro

1992 ◽  
Vol 262 (3) ◽  
pp. F442-F448 ◽  
Author(s):  
N. S. Krieger ◽  
N. E. Sessler ◽  
D. A. Bushinsky
Keyword(s):  
Alkaline Phosphatase ◽  
Metabolic Acidosis ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Collagen Synthesis ◽  
Calcium Flux ◽  
Osteoclastic Activity ◽  
Glucuronidase Activity

Metabolic acidosis induces net calcium flux (JCa) from cultured neonatal mouse calvariae through physicochemical and cell-mediated mechanisms. To determine the role of osteoblasts in acid-induced JCa, collagen synthesis and alkaline phosphatase activity were assessed in calvariae incubated in reduced pH and bicarbonate medium, a model of metabolic acidosis (Met), and compared with controls (Ctl). Collagen synthesis fell from 30.5 +/- 1.1 in Ctl to 25.1 +/- 0.4% with Met, and alkaline phosphatase decreased from 403 +/- 25 in Ctl to 298 +/- 21 nmol Pi.min-1.mg protein-1 with Met. During acidosis JCa was correlated inversely with percent collagen synthesis (r = -0.743, n = 11, P = 0.009) and with alkaline phosphatase activity (r = -0.453, n = 22, P = 0.034). To determine the role of osteoclasts in acid-induced JCa, osteoclastic beta-glucuronidase activity was determined in Ctl and Met in the absence or presence of the osteoclastic inhibitor calcitonin (CT, 3 x 10(-9) M). Met increased beta-glucuronidase (5.9 +/- 0.2) compared with Ctl (4.6 +/- 0.3 micrograms phenolphthalein released.bone-1.h-1), whereas CT inhibited beta-glucuronidase in both Ctl and Met (3.1 +/- 0.2 and 3.5 +/- 0.3, respectively). During acidosis JCa was correlated directly with beta-glucuronidase activity (r = 0.683, n = 42, P less than 0.001). Thus the cell-mediated component of JCa during acidosis in vitro appears to result from a combination of inhibited osteoblastic and stimulated osteoclastic activity.

Increased, Directed Osteoblast Adhesion at Nanophase Ti and Ti6Al4V Particle Boundaries

2003 ◽  
Vol 806 ◽  
Author(s):  
Thomas J. Webster ◽  
Jeremiah U. Ejiofor
Keyword(s):  
Carbon Nanofibers ◽  
Metal Particle ◽  
Glycolic Acid ◽  
In Vitro Study ◽  
Vitro Study ◽  
Orthopedic Implant ◽  
Grain Sizes ◽  
Nanophase Materials ◽  
Osteoblast Adhesion

ABSTRACTIncreased functions of osteoblasts (bone-forming cells) have been demonstrated on nanophase compared to conventional ceramics (specifically, alumina, titania, and hydroxyapatite), polymers (such as poly-lactic-glycolic acid and polyurethane), carbon nanofibers, and composites thereof. Nanophase materials are materials that simulate dimensions of constituent components of bone since they possess particle or grain sizes less than 100 nm. However, to date, interactions of osteoblasts on nanophase compared to conventional metals remain to be elucidated. For this reason, the objective of the present in vitro study was to design, fabricate, and evaluate osteoblast adhesion on nanophase metals (specifically, Ti and Ti6Al4V). Results of this study provided the first evidence of increased osteoblast adhesion on nanophase compared to conventional Ti-based metals. Moreover, directed osteoblast adhesion was observed preferentially at metal particle boundaries. It is speculated that since more particle boundaries were created through the use of nanophase compared to conventional metals, increased osteoblast adhesion resulted. Because adhesion is a necessary prerequisite for subsequent functions of osteoblasts (such as deposition of calcium-containing mineral), the present study suggests that Ti-based nanophase metals should be further considered for orthopedic implant applications.

scholarly journals Encapsulation of Nicardipine Hydrochloride and Release from Biodegradable Poly(D,L-lactic-co-glycolic acid) Microparticles by Double Emulsion Process: Effect of Emulsion Stability and Different Parameters on Drug Entrapment

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Nopparuj Soomherun ◽  
Narumol Kreua-ongarjnukool ◽  
Sorayouth Chumnanvej ◽  
Saowapa Thumsing
Keyword(s):  
Drug Delivery ◽  
Controlled Release ◽  
Glycolic Acid ◽  
Release Kinetics ◽  
Double Emulsion ◽  
Average Diameter ◽  
Optimal Processing ◽  
Alternative Drug ◽  
Biodegradable Poly

Poly(D,L-lactic-co-glycolic acid) (PLGA) is an important material used in drug delivery when controlled release is required. The purpose of this research is to design and characterize PLGA microparticles (PLGA MPs) implants for the controlled release of nicardipine hydrochloride (NCH) in vitro. This study used the water-in-oil-in-water (w1/o/w2) double emulsion and solvent diffusion/evaporation approach to prepare PLGA MPs. Optimal processing conditions were found, such as polymer content, surfactant type, stabilizer concentration, inner and outer aqueous phase volumes, and stirring speed. The PLGA MPs for use as nicardipine hydrochloride (NCH) loading and release had spherical morphology, and the average diameter was smaller than 5.20±0.25 μm. The release kinetics were modeled to elucidate the possible mechanism of drug release. In vitro release studies indicated that the NCH release rate is slow and continuous. PLGA MPs are an interesting alternative drug delivery system, especially for use with NCH for biomedical applications.

scholarly journals Modulating Mesenchymal Stem Cell Behavior Using Human Hair Keratin-Coated Surfaces

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Pietradewi Hartrianti ◽  
Ling Ling ◽  
Lyn Mei Ming Goh ◽  
Kok Seng Amos Ow ◽  
Rebekah Margaret Samsonraj ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Human Hair ◽  
Ex Vivo ◽  
The Body ◽  
Animal Origin ◽  
Homogeneous Distribution ◽  
Tissue Culture Polystyrene ◽  
Coated Surfaces ◽  
Hair Keratins

Human mesenchymal stem cells (hMSCs) have shown great potential for therapeutic purposes. However, the low frequencies of hMSCs in the body and difficulties in expanding their numbersin vitrohave limited their clinical use. In order to develop an alternative strategy for the expansion of hMSCsin vitro, we coated tissue culture polystyrene with keratins extracted from human hair and studied the behavior of cells from 2 donors on these surfaces. The coating resulted in a homogeneous distribution of nanosized keratin globules possessing significant hydrophilicity. Results from cell attachment assays demonstrated that keratin-coated surfaces were able to moderate donor-to-donor variability when compared with noncoated tissue culture polystyrene. STRO-1 expression was either sustained or enhanced on hMSCs cultured on keratin-coated surfaces. This translated into significant increases in the colony-forming efficiencies of both hMSC populations, when the cells were serially passaged. Human hair keratins are abundant and might constitute a feasible replacement for other biomaterials that are of animal origin. In addition, our results suggest that hair keratins may be effective in moderating the microenvironment sufficiently to enrich hMSCs with high colony-forming efficiencyex vivo, for clinical applications.

scholarly journals Impregnation of Curcumin into a Biodegradable (Poly-lactic-co-glycolic acid, PLGA) Support, to Transfer Its Well Known In Vitro Effect to an In Vivo Prostate Cancer Model

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2312 ◽  
Author(s):  
Eulalio Gracia ◽  
Andrea Mancini ◽  
Alessandro Colapietro ◽  
Cristina Mateo ◽  
Ignacio Gracia ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Glycolic Acid ◽  
Curcuma Longa ◽  
Subcutaneous Administration ◽  
Uv Spectrophotometry ◽  
Medicinal Plant Extracts ◽  
Biodegradable Poly ◽  
Vitro Effect

Prostate cancer (PCa) is one of the most common cancers in older men and is associated with high mortality. Despite advances in screening for early detection of PCa, a large proportion of patients continue to be diagnosed with metastatic disease, with ~20% of men showing a high tumor grade and stage. Medicinal plant extracts have a great potential to prevent/treat PCa, as well as to reduce its incidence/prevalence and improve survival rates. One of the most promising extracts is curcumin, which is a major, nontoxic, bioactive compound of Curcuma longa. Curcumin has strong antitumor activity in vitro. However, its potential beneficial in vivo affects are limited by its low intestinal absorption and rapid metabolism. In this study, curcumin was impregnated into a biodegradable poly(lactic-co-glycolic) acid (PLGA) support and characterized by FTIR and DSC, and its release by UV spectrophotometry. PLGA-curcumin was tested in different subcutaneous PCa xenograft models (PC3, 22rv1, and DU145 PCa cell-lines), and its effects evaluated by tumor progression an immuno-histochemical analysis (Trichromic, Ki67 and TUNEL stainings), were compared with those of a commercial curcumin preparation. Our results indicate that curcumin-impregnated PLGA is significantly more active (~2-fold increase) with respect to oral curcumin, which supports its use for subcutaneous administration.

Increased In Vivo Collagen Synthesis and In Vitro Cell Proliferative Effect of Glycolic Acid

1998 ◽  
Vol 24 (10) ◽  
pp. 1054-1058 ◽  
Author(s):  
SEONG-JIN KIM ◽  
JONG-HYUK PARK ◽  
DO-HYUN KIM ◽  
YOUNG-HO WON ◽  
HOWARD I. MAIBACH
Keyword(s):  
Collagen Synthesis ◽  
Glycolic Acid ◽  
Proliferative Effect

scholarly journals In vitro and in vivo release of nerve growth factor from biodegradable poly-lactic-co-glycolic-acid microspheres

2010 ◽  
Vol 95A (4) ◽  
pp. 1067-1073 ◽  
Author(s):  
Ralph de Boer ◽  
Andrew M. Knight ◽  
Robert J. Spinner ◽  
Martijn J. A. Malessy ◽  
Michael J. Yaszemski ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Glycolic Acid ◽  
Nerve Growth ◽  
In Vivo Release ◽  
Biodegradable Poly
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