Interfacing Conducting Polymers and Biological Macromolecules: A Case Study of Insecticide Biosensor Development

1993 ◽  
Vol 330 ◽  
Author(s):  
Kenneth Marx ◽  
Madhu Ayyagari ◽  
Sanjay Kamtekar ◽  
Rajiv Pande ◽  
Jeong Ok Lim ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Smart Materials ◽  
Enzyme Inhibitors ◽  
Hydrophobic Surface ◽  
Biological Macromolecules ◽  
Glass Capillary ◽  
Alkyl Chains ◽  
Flexible System ◽  
Conducting Copolymers

ABSTRACTThe creation of smart materials is a current goal of many laboratories including our own. We are involved in studying a number of thin film and monolayer systems which share the common features of involving highly conjugated conducting organic polymers and the evolved properties of specific biological macromolecules. In this report we describe a generic ‘cassette’ methodology for immobilizing biotinylated biological macromolecules to hydrophobic surfaces using a novel class of conducting copolymers of polythiophene. These copolymers are derivatized with long alkyl chains and biotin moieties to bind, respectively, to the hydrophobic surface and the biotinylated species, through the biotin - streptavidin interaction. We utilize the monolayer ‘cassette’ approach to attach a signal transducing biomolecule alkaline phosphatase to the surface of a glass capillary. This produces a flexible system in which chemiluminescence provides the basis for a useful measurement strategy. A novel technique involving the generation of chemiluminescence signal from alkaline phosphatase catalyzed dephosphorylation of a macrocyclic phosphate compound is described. Detection of paraoxon and methyl parathion, both enzyme inhibitors, has been achieved at ppb levels. The technique is rapid, sensitive, and is applicable to the detection of all organophosphorus based insecticides. The technique will be used in developing a fiber;optic biosensor for remote detection of insecticides.

Embodied interactions: Towards an exploration of the expressive and narrative potential of performance costume through wearable technologies

Scene ◽  
2014 ◽  
Vol 2 (1) ◽  
pp. 179-196 ◽  
Author(s):  
Sofia Pantouvaki
Keyword(s):  
Smart Materials ◽  
Costume Design ◽  
The Body ◽  
Wearable Electronics ◽  
Live Performance ◽  
Wearable Technologies ◽  
Opening Ceremony ◽  
Interactive Interfaces ◽  
Specific Fragment

The use of smart materials and wearable electronics has rapidly expanded in the field of fashion, introducing new interactive qualities of surfaces, materials and garments. In fashion garments, the performative environment functions as an abstract site for experimentation, expression and communication of the wearer through the intelligent garment. However, there is still limited use of embodied technologies in the field of performance costume for text-based and music-based performance, with the exception of integrated lighting technologies, currently broadly used in musical performance. This article provides a critical review of specific examples of technology-led garments in live performance, and uses a specific fragment from the Athens 2004 Olympic Games Opening Ceremony as a case study to highlight how technologies embedded in costume can create interactive interfaces between the body of the performer and the environment – the space, the other performers, the audience – becoming a transmitter and receiver of emotions, experiences and meanings in innovative ways. By analysing this case, as well as by posing questions, this article aims at generating a discourse on the expressive and narrative potential of the use of intelligent materials and embodied technologies within the creative practice of costume design.

scholarly journals Implementing CCTV-Based Attendance Taking Support System Using Deep Face Recognition: A Case Study at FPT Polytechnic College

Symmetry ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 307 ◽  
Author(s):  
Ngo Tung Son ◽  
Bui Ngoc Anh ◽  
Tran Quy Ban ◽  
Le Phuong Chi ◽  
Bui Dinh Chien ◽  
...  
Keyword(s):  
Face Recognition ◽  
Motion Blur ◽  
Security Monitoring ◽  
Flexible System ◽  
Lighting Conditions ◽  
Satisfactory Performance ◽  
Measurement Results ◽  
The Face ◽  
Recognition Systems

Face recognition (FR) has received considerable attention in the field of security, especially in the use of closed-circuit television (CCTV) cameras in security monitoring. Although significant advances in the field of computer vision are made, advanced face recognition systems provide satisfactory performance only in controlled conditions. They deteriorate significantly in the face of real-world scenarios such as lighting conditions, motion blur, camera resolution, etc. This article shows how we design, implement, and conduct the empirical comparisons of machine learning open libraries in building attendance taking (AT) support systems using indoor security cameras called ATSS. Our trial system was deployed to record the appearances of 120 students in five classes who study on the third floor of FPT Polytechnic College building. Our design allows for flexible system scaling, and it is not only usable for a school but a generic attendance system with CCTV. The measurement results show that the accuracy is suitable for many different environments.

scholarly journals RCSB Protein Data Bank tools for 3D structure-guided cancer research: human papillomavirus (HPV) case study

Oncogene ◽  
2020 ◽  
Vol 39 (43) ◽  
pp. 6623-6632
Author(s):  
David S. Goodsell ◽  
Stephen K. Burley
Keyword(s):  
Human Papillomavirus ◽  
Protein Data Bank ◽  
3D Structure ◽  
Healthcare Providers ◽  
Data Bank ◽  
Data Repository ◽  
Biological Macromolecules ◽  
Biologic Drugs ◽  
Related Proteins

Abstract Atomic-level three-dimensional (3D) structure data for biological macromolecules often prove critical to dissecting and understanding the precise mechanisms of action of cancer-related proteins and their diverse roles in oncogenic transformation, proliferation, and metastasis. They are also used extensively to identify potentially druggable targets and facilitate discovery and development of both small-molecule and biologic drugs that are today benefiting individuals diagnosed with cancer around the world. 3D structures of biomolecules (including proteins, DNA, RNA, and their complexes with one another, drugs, and other small molecules) are freely distributed by the open-access Protein Data Bank (PDB). This global data repository is used by millions of scientists and educators working in the areas of drug discovery, vaccine design, and biomedical and biotechnology research. The US Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB) provides an integrated portal to the PDB archive that streamlines access for millions of worldwide PDB data consumers worldwide. Herein, we review online resources made available free of charge by the RCSB PDB to basic and applied researchers, healthcare providers, educators and their students, patients and their families, and the curious public. We exemplify the value of understanding cancer-related proteins in 3D with a case study focused on human papillomavirus.

Design of a Compact Actuated Compliant Elbow Joint

2014 ◽  
Vol 14 (08) ◽  
pp. 1440030 ◽  
Author(s):  
Johannes Gerard Kleinjan ◽  
Alje Geert Dunning ◽  
Justus Laurens Herder
Keyword(s):  
Smart Materials ◽  
Performance Indicators ◽  
Elbow Joint ◽  
Large Deflection ◽  
Rotational Axis ◽  
Half Cycle ◽  
Compliant Joints ◽  
Compliant Joint ◽  
Cycle Efficiency

Compactness is a valuable property in designs of assistive devices and exoskeletons. Current devices are large and stigmatizing in the eyes of the users. The cosmetic appearance will increase by reducing the size. The users want a device that is small enough to be worn underneath the clothes, so it becomes unnoticeable. The goals of this paper are (1) to provide an overview of the shape-changing-material-actuated large-deflection compliant rotational joints, (2) provide new introduced performance indicators that evaluate the designs on performance with respect to volume or weight and (3) design a compact active assistive elbow device as a case study. In order to reach these goals, two evolving fields of study are brought together that have great potential to reduce the size of exoskeletons: smart materials and compliant rotational joints. Smart materials have the ability to change their shape, which make them suitable as actuators. Compliant joints can be compact, since they are made out of one piece of material. An overview of shape-changing-material-actuated large-deflection compliant rotational joints is presented. Performance indicators are proposed to evaluate the existing designs and the prototype. As a case study a compact actuated rotational elbow joint is presented. An antagonistic actuator made from shape memory alloy wires is able to carry an external load and to actuate to move the arm to different positions. The compliant joint is optimized to balance the weight of the arm and to auto-align with the rotational axis of the human elbow joint. A prototype is able to generate a volume specific stall torque of 5.77 ⋅ 103 Nm/m3, produces a work density of 7.27 ⋅ 103 J/m3 based on volumes including isolation covers and the half-cycle efficiency of the device is 3.6%. The prototype is able to balance and actuate a torque of 1.1 Nm.

scholarly journals Potret dan Dinamika Wirausaha Muda Kreatif Kaliabu

2019 ◽  
Vol 6 (2) ◽  
pp. 636
Author(s):  
Onto Kusumo ◽  
Dewi Cahyani Puspitasari
Keyword(s):  
Graphic Design ◽  
Financial Services ◽  
Economic Benefits ◽  
Creative Class ◽  
Economic Changes ◽  
Study Approach ◽  
Flexible System ◽  
Cross Country ◽  
The Village

This study aimed to understand the emergence of the creative class in the context of youth villager. This study used a case study approach by taking the case of the emergence of graphic designers at Kaliabu Village, Magelang Regency. This study used the theory of Creative Class and Youth to explain the dynamics of the emergence of the creative class in Kaliabu. Opportunities in the form of professional graphic designers who comes in line with the current socio-economic changes currently utilized by the younger generation Kaliabu. These changes are related to the economic system based on information technology, progress and financial services industry, the emergence of the company with a flexible system, global supply chains, as well as the process of globalization. These things are external factors that allow graphic designers in Kaliabu Village to carry out cross-country service transactions in online graphic design marketplaces such as 99design.The external change then responded with passion of high exploration by youths Kaliabu. The success of one of the residents in Kaliabu by winning the online design contest fortunately inspire other youths. The learning process occurs among Kaliabu youth. Ultimately in 2012 in the village of Kaliabu already has two hundred young people who become a graphic designer. They are embodied in a community called as Rewo-Rewo. Their function as provider of graphic design and the relations on aspects of talent, technology, and tolerance explaining they are parts of the creative classes. The conclusion of this study showed that the rise of the creative class in the form of hundreds of graphic designers in Kaliabu is a result of the dynamics of youth Kaliabu in response to socio-economic changes. The graphic design of the logo brought economic benefits to them.

Hydrophobic Surface Coating of Nanodiamonds by Polyglycerol‐Based Polymers with Alkyl Chains for Dispersing in an Organic Solvent

ChemNanoMat ◽  
2020 ◽  
Vol 6 (9) ◽  
pp. 1332-1336
Author(s):  
Wei Wang ◽  
Yajuan Zou ◽  
Alejandro López‐Moreno ◽  
Yujing Jiang ◽  
Feng Wen ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Surface Coating ◽  
Hydrophobic Surface ◽  
Alkyl Chains

scholarly journals Toxicological effects of a herbicide – Pendinmethalin: a case study of Kenana Sugar Industry, White Nile Province, Sudan

2014 ◽  
Vol 3 (2) ◽  
pp. 258-262
Author(s):  
Muawia IA Abdelgadir ◽  
Murwan K Sabahelkhier ◽  
Mohamed A Adris ◽  
Omer F Idris ◽  
Shama IY Adam ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Wistar Rats ◽  
Sugar Industry ◽  
High Dose ◽  
Toxicological Effects ◽  
Impaired Function ◽  
White Nile ◽  
High Doses ◽  
Phosphatase Enzyme

The aim of this experiment is to know the toxicity of high uses of pendimethalin as herbicide used in Kenana Sugar Industry, period of experiment is 30 days. The results were reveal that there was significantly effect of high doses of pendimethalin on the liver of Wistar rats, because increase in the dose of the pendimethalin reflected by increasing the alkaline phosphatase enzyme during increase the period of time. In addition there were histopathological medical in cells of the liver of Wistar rats, which lead to impaired function of the liver, so it is very clear observed in water collected from Kenana Sugar Industry plate two, and 375 and 750 (plate three and four) compared with plate one( free from pendimethalin treatment. This experiment indicated that high dose of pendimethalin causes toxicity. DOI: http://dx.doi.org/10.3126/ije.v3i2.10638 International Journal of the Environment Vol.3(2) 2014: 258-262

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