Surface-Enhanced Raman Spectroscopy from Colloids at High Pressures

1992 ◽  
Vol 289 ◽  
Author(s):  
Michael Bradley ◽  
John Krech ◽  
Shlomo Efrima
Keyword(s):  
High Pressures ◽  
Colloidal Suspension ◽  
Blue Shift ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Spectrum ◽  
Frequency Shifts ◽  
Pressure Surface ◽  
Surface Enhanced ◽  
Anisic Acid ◽  
Surface Enhanced Raman

AbstractHigh pressure surface enhanced Raman (SERS) spectra are reported for a highly dense silver colloidal suspension, termed a MEtal Liquid-Like Film or MELLF. Comparison is made with the SERS spectrum of a dilute organosol. Raman signals due to adsorbed molecules and the solvent dichloromethane reveal appreciable frequency shifts as the colloidal environment is influenced by temperature, pressure, and packing. The C-Cl stretch of neat dichloromethane near 705 cm−1 shifts 1.7 cm −1 blue in a MELLF. Raman signals due to the adsorbed anisic acid in MELLFs blue shift about 2 cm −1 as 4 kilobars of pressure are applied; the solvent peaks shift blue only about 1 cm −1. Temperature influences the MELLF in two ways: desorption of anisic acid and formation of agglomerated particles.

scholarly journals Surface-Enhanced Raman Spectroscopy of Polyelectrolyte-Wrapped Gold Nanoparticles in Colloidal Suspension

2013 ◽  
Vol 117 (20) ◽  
pp. 10677-10682 ◽  
Author(s):  
Sean T. Sivapalan ◽  
Brent M. DeVetter ◽  
Timothy K. Yang ◽  
Matthew V. Schulmerich ◽  
Rohit Bhargava ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Raman Spectroscopy ◽  
Colloidal Suspension ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Spectroelectrochemical Investigation of the Interaction of Adenine with Pyridoxine at Physiological pH

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Yasmin Roye ◽  
Uche Udeochu ◽  
Maraizu Ukaegbu ◽  
Jonathan Onuegbu
Keyword(s):  
Raman Band ◽  
Bathochromic Shift ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Shift ◽  
Sers Spectrum ◽  
Visible Absorption ◽  
Adenine Ring ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
The Individual

Spectroelectrochemical techniques were used to probe the interaction of adenine with pyridoxine at pH 7.0. Analysis of UV-visible absorption of the adenine-pyridoxine complex at 260 nm using the Lineweaver–Burk double reciprocal plot produced a linear graph indicating a 1 : 1 mode of interaction between the compounds and a binding constant of 29.1. Change in the background current and broadening of adenine and pyridoxine cyclic voltammetry (CV) oxidation peaks at 1.0 V and 0.8 V, respectively, compared to the CV of the individual compounds is indicative of an interaction. The Raman shift of the coupled –C(11)H2-OH bending and in-plane N(7)-H mode at 1235 cm−1 to 1215 cm−1 of pyridoxine and the shift to the lower wavenumber of the adenine –N(10)H2 rocking band from 942 to 906 cm−1 confirm that the adenine exocyclic amino group and its purine nitrogen atom N(7) interacts with pyridoxine O(12) via the formation of hydrogen bonds. Strong enhancement of surface-enhanced Raman spectroscopy (SERS) bands pertaining to adenine and the bathochromic shift of the normal Raman band due to the adenine ring breathing mode observed at 722 cm−1 in the spectrum of adenine, to 732 cm−1 in the SERS spectrum of aqueous adenine-pyridoxine indicates that the complex adsorbs onto the Ag nanoparticle surface with the adenine portion possessing a perpendicular orientation.

Surface-Enhanced Raman Spectroscopy as a Detection Method in Gas Chromatography

1994 ◽  
Vol 48 (10) ◽  
pp. 1193-1195 ◽  
Author(s):  
E. Roth ◽  
W. Kiefer
Keyword(s):  
Gas Chromatography ◽  
Detection Method ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Spectrum ◽  
Static System ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Tlc Plates ◽  
On Line ◽  
Enhanced Raman Scattering

The use of surface-enhanced Raman scattering (SERS) as a detection method in gas chromatography (GC) was investigated by two different approaches; GC eluates were trapped either in liquid silver sol or on solid thin-layer chromatography (TLC) plates, previously coated with silver colloidal solution. Subsequently, the trapped analytes were monitored by their SERS spectrum. Pyridine was successfully used as probe molecule for both interfaces. The extension of this static system for application in on-line detection of GC eluates is discussed.

scholarly journals SERS study on myeloperoxidase and its immunocomplex: Identification of binding interactions

2010 ◽  
Vol 24 (3-4) ◽  
pp. 183-190
Author(s):  
Elisabeth S. Papazoglou ◽  
Sundar Babu ◽  
David R. Hansberry ◽  
Sakya Mohapatra ◽  
Chirag Patel
Keyword(s):  
Amino Acids ◽  
Raman Spectroscopy ◽  
Conformational Changes ◽  
Surface Enhanced Raman Spectroscopy ◽  
Biological Molecules ◽  
Sers Spectrum ◽  
Amino Acid Residues ◽  
Igg Antibodies ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Surface Enhanced Raman Spectroscopy (SERS) has demonstrated significant benefit in the identification of biological molecules. In this paper we have examined how to identify and differentiate the 150 kDa protein myeloperoxidase (MPO) from its corresponding antibody (Ab) and their immunocomplex through the use of SERS. The SERS signal of these biological molecules was enabled by 40 nm gold nanoparticles. The SERS spectra for both MPO and the Ab (an IgG molecule) demonstrated results consistent with previous published work on the Raman spectra of MPO and IgG antibodies. The immunocomplex SERS spectra showed peak shifts and intensity variations that could be attributed to conformational changes that occur during immunocomplex formation. Several key spectral areas have been identified which correspond to specific amino acids being shielded from undergoing resonance while new amino acid residues are made visible in the SERS spectrum of the immunocomplex and could be a result of conformational binding. These results indicate that SERS can be used to identify binding events and distinguish an immunocomplex from its individual components.

scholarly journals Identifying and detecting Entomopathogenic fungi using Surface-enhanced Raman spectroscopy / Identificação e detecção de fungos entomopatogênicos utilizando Superficie-enhanced Raman espalhamento

2021 ◽  
Vol 4 (4) ◽  
pp. 4833-4851
Author(s):  
Javier Christian Ramirez Perez ◽  
Tatiana Alves Dos Reis ◽  
Marcia de Almeida Rizzutto
Keyword(s):  
Raman Spectroscopy ◽  
Entomopathogenic Fungi ◽  
Insect Pests ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Spectrum ◽  
Natural Ecosystem ◽  
Fungal Entomopathogens ◽  
Short Period ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

In the natural ecosystem, fungal entomopathogens are the most efficient biocontrol agents against insect pests. In this study we offer an alternative for conventional fungal diagnostic, Surface-enhanced Raman spectroscopy (SERS) technique combine with principal component analysis (PCA) for detection and identification three entomopathogenic fungi, namely, IBCB 66 Beauveria bassiana, IBCB 130 Isaria fumosorosea, and IBCB 425 Metarhizium anisopliae. Using a simple preparation approach, highly active silver nanoparticles suitable for detecting complex biomolecules were produced for application in the SERS technique. Entomopathogens fungi produced highly enhanced and reproducible Raman signals based on their biochemical composition due to the high density of hot spots at the confluence of silver nano-aggregates, allowing the three entomopathogens species to be differentiated in the SERS spectrum fingerprint region, 550-1700 cm-1. The SERS method, along with PCA analysis, accounted for over 99 % of total variance and allowed for very high probability discrimination between the three entomopathogens, allowing taxonomic affiliation to be determined in a short period of time.  These findings suggest that the SERS methodology can be used to develop a new, fast, accurate, and cost-effective diagnostic method for fungal entomopathogens.

Potential-Averaged Surface-Enhanced Raman Spectroscopy

1996 ◽  
Vol 50 (12) ◽  
pp. 1569-1577 ◽  
Author(s):  
Z. Q. Tian ◽  
W. H. Li ◽  
B. W. Mao ◽  
S. Z. Zou ◽  
J. S. Gao
Keyword(s):  
Raman Spectroscopy ◽  
Active Sites ◽  
Single Channel ◽  
Pulse Height ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Spectrum ◽  
Surface Species ◽  
Electrode Potentials ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

This paper describes a novel technique called potential-averaged surface-enhanced Raman spectroscopy (PASERS) which has several advantages over SERS. A PASERS spectrum is acquired when the electrode is rapidly modulated between two potentials by applying a square-wave voltage. The potential-averaged SERS spectrum contains all the information on the surface species at the two modulated potentials, and each individual SERS spectrum can then be extracted by deconvolution. By properly choosing the two modulating potentials, one can obtain SERS spectra of surface species at electrode potentials where SERS-active sites are normally unstable. PASERS also leads to a unique way of studying complex interfacial kinetic processes by controlling the voltage pulse height, frequency, and shape. Moreover, the measurement of time-resolved spectra in the very low vibrational frequency region can be achieved by PASERS with the use of a conventional scanning spectrometer with a single-channel detector. In this paper, the main advantages of PASERS are illustrated by studying two typical SERS systems, i.e., thiocyanate ion and thiourea adsorbed at silver electrodes, respectively. It is shown that the potential-averaging method can be applied as a common method to many other existing spectroelectrochemical techniques.

scholarly journals Waveguide excitation and collection of surface-enhanced Raman scattering from a single plasmonic antenna

Nanophotonics ◽  
2018 ◽  
Vol 7 (7) ◽  
pp. 1299-1306 ◽  
Author(s):  
Frédéric Peyskens ◽  
Pieter Wuytens ◽  
Ali Raza ◽  
Pol Van Dorpe ◽  
Roel Baets
Keyword(s):  
Signal To Noise Ratio ◽  
Single Mode ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Spectrum ◽  
Plasmonic Enhancement ◽  
Detection Scheme ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
On Chip

AbstractThe integration of plasmonic antennas on single-mode silicon nitride waveguides offers great perspective for integrated surface-enhanced Raman spectroscopy (SERS). However, the few reported experimental demonstrations still require multiple plasmonic antennas to obtain a detectable SERS spectrum. Here, we show, for the first time, SERS signal detection by a single nanoplasmonic antenna integrated on a single-mode SiN waveguide. For this purpose, we investigated a backscattering detection scheme in combination with background noise reduction, which allowed an optimization of the signal-to-noise ratio (SNR) of this platform. Furthermore, a comparison with the free-space SERS spectrum of the same antenna shows that the conversion efficiency from pump power to total radiated Stokes power is twice as efficient in the case of waveguide excitation. As such, we explored several important aspects in the optimization of on-chip SERS sensors and experimentally demonstrated the power of exciting nanoplasmonic antennas using the evanescent field of a waveguide. This observation not only is useful for Raman sensing but also could be beneficial for any process involving plasmonic enhancement.

scholarly journals Optimizing electroporation assisted silver nanoparticle delivery into living C666 cells for surface-enhanced Raman spectroscopy

2011 ◽  
Vol 25 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Yun Yu ◽  
Juqiang Lin ◽  
Yanan Wu ◽  
Shangyuan Feng ◽  
Yongzeng Li ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Spectrum ◽  
Electric Pulses ◽  
Nanoparticle Delivery ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Delivery Efficiency

Electroporation assisted metallic nanoparticle delivery has been shown by our previous work to significantly reduce the time of sample preparation for surface-enhanced Raman spectroscopy (SERS) measurements of biological cells. In this paper, we report our experimental work to optimize the electroporation parameters, including adjustment of the pulse pattern, operation temperature, and electroporation buffer, for fastest delivery of silver nanoparticles into living C666 cells (a human nasopharyngeal carcinoma cell line). The delivery efficiency was evaluated by the integrated intensity of whole cell SERS spectrum. Our work concluded that the silver nanoparticle delivery rate is best under the electroporation condition of using 4 consecutive 350 V (875 V/cm) rectangular electric pulses of 1, 10, 10 and 1 ms durations, respectively. Low temperature (0–4°C) is necessary for keeping cell viability during the electroporation process and it also improves the delivery efficiency of silver nanoparticles. The serum in the buffer has no obvious effect on the delivery efficiency.

scholarly journals Fabrication of Biosensor Based on Polyaniline/Gold Nanorod Composite

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Uğur Tamer ◽  
Ali İhsan Seçkin ◽  
Erhan Temur ◽  
Hilal Torul
Keyword(s):  
Glucose Oxidase ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Gold Nanorod ◽  
Sers Spectrum ◽  
Pani Film ◽  
Amperometric Response ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

This present paper describes a new approach to fabricate a new amperometric sensor for the determination of glucose. Polyaniline (PANI) film doped with colloidal gold nanorod particles has been used to immobilize glucose oxidase by glutaraldehyde. The polyaniline/gold nanorod composite structure gave an excellent matrix for enzyme immobilization due to the large specific surface area and higher electroactivity. The composite has been characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), and surface-enhanced Raman spectroscopy (SERS). The SERS spectrum of the surface-immobilized glucose oxidase and the spectrum of the native enzyme indicate that the main feature of the native structure of glucose oxidase was conserved after being immobilized on the polymer matrix. The amperometric response was measured as a function of concentration of glucose at a potential of 0.6 V versus Ag/AgCl in 0.1 M phosphate buffer at pH 6.4. Linear range of the calibration curve was from 17.6 μM to 1 mM with a sensitivity of 13.8 μA⋅mM−1⋅cm−2and a limit of detection (LOD) of 5.8 μM. The apparent Michaelis-Menten constantKMwas calculated as 1.0 mM and the response time was less than 3 seconds.

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