Forgery Determination by Functional Analysis

1992 ◽  
Vol 267 ◽  
Author(s):  
Leonard Gorelick ◽  
A.J. Gwinnett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Functional Analysis ◽  
Drill Holes ◽  
Scanning Electron ◽  
Made In

ABSTRACTStone statuettes from turkey in simply shaped animal and bird forms were analyzed using scanning electron microscopy. observations made in drill holes in some of the artifacts led to definite unequivocal evidence for forgery while others were equivocal.

scholarly journals Physical and mechanical properties of membrane polyvinilidene flouride with the addition of silver nitrate

2018 ◽  
Vol 156 ◽  
pp. 08014
Author(s):  
Agung Mataram ◽  
S. Rizal ◽  
Estu Pujiono
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Silver Nitrate ◽  
Pore Diameter ◽  
Standardized Test ◽  
Physical And Mechanical Properties ◽  
Mixed Composition ◽  
Scanning Electron ◽  
Made In

The membrane is a layer formed from fine fibers are used as a filter. In this research, measured the tensile strength and pore size of the membrane that is made in three variations of a specimen with a mixed composition of different Polyvinilidene Fluoride 17.5%, 20%, 22.5%., membrane made using Polyvinilidene Fluoride polimer granules and N,N-Dimethylformamide as solvent and silver nitrate. Membrane molded into standardized test specimens and tested with a device Adhesion Tearring Strength Tester. The results show an increase in the value of the tensile test of 404.8356 KPa to the composition of 17.5%, 507.3598 KPa for composition of 20% and 603.7218 KPa to a composition of 22.5%. For testing the microstructure using a Scanning Electron Microscopy with the resulting increase in pore diameter, for the range of 558.4 nm-781.8 nm are void to the composition of 17.5%, range 670.1 nm - 781.8 nm without voids for the composition of 20% and 1.117 μm-1.228μm without voids on a composition of 22.5

Scanning Electron Microscopy of the Normal Rabbit Hepatocyte

1975 ◽  
Vol 33 ◽  
pp. 520-521
Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Inferior Vena ◽  
Healthy Adult ◽  
Three Dimensional ◽  
Vena Cava ◽  
Tissue Preparation ◽  
Renal Veins ◽  
Scanning Electron ◽  
Made In

Current interest in the ultrastructure of the liver arises partly from demonstrations that scanning electron microscopy is very useful for studying surfaces and interrelationships of intrahepatic structures in both normal and pathological situations. In recent years, the development of the scanning electron microscope and techniques of tissue preparation has provided a three-dimensional method for examination of the features of intrahepatic ultrastructure. Only reports on the rat liver were available. However, SEM observations on the liver from other species are currently in progress in our laboratories. In this report, the SEM observations on the rabbit hepatocyte and the relevant technique for preparing hepatic tissues are described.Healthy, adult New Zealand rabbits were anesthetized by either intravenous or intraperitoneal injection of pentobarbital. Both abdominal and thoracic walls of anesthetized animals were opened. A small incision was made in the inferior vena cava at the level just below the renal veins, immediately followed by intracardiac perfusion of chilled 0.9% saline for 5 minutes.

Scanning electron microscopy 1928-1965

1993 ◽  
Vol 51 ◽  
pp. 762-763 ◽  
Author(s):  
D. McMullan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Near Field ◽  
Optical Microscope ◽  
Cambridge University ◽  
Engineering Department ◽  
Near Field Scanning ◽  
Scanning Electron ◽  
Made In

The beginning of the general use of the scanning electron microscope (SEM) can be accurately dated to 1965 when the Cambridge Instrument Company in the U.K. marketed their Stereoscan 1 SEM (to be followed about 6 months later by JEOL in Japan). But development had been in progress intermittently over the previous 30 years in Germany, the U.S.A., France, and the U.K. where in 1948 work was begun in Charles Oatley’s department at the Cambridge University Engineering Department which led directly to the Stereoscan. The purpose of this paper is to trace the development of the SEM and to show that some of the ideas pul forward by the early workers were well ahead of their time and only became technologically practicable much later.The First proposal for the application of scanning to microscopy was made in 1928 by Synge, an Irish scientific dilettante, who conceived the near-field scanning optical microscope but did not attempt to build one.

scholarly journals Scale ontogeny in the cardinalfish family Apogonidae

Zootaxa ◽  
2016 ◽  
Vol 4196 (1) ◽  
pp. 107 ◽  
Author(s):  
OFER GON
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sister Group ◽  
Scale Morphology ◽  
Scale Type ◽  
Molecular Studies ◽  
Goby Species ◽  
Ctenoid Scale ◽  
Scanning Electron ◽  
Made In

Following the discovery of spinoid scales in species of the cardinalfish genus Siphamia, a survey of 20 apogonid genera, using scanning electron microscopy, found that scale ontogeny in the Apogonidae usually proceeds along three phases, cycloid, spinoid and transforming ctenoid, that develop in that order. The transforming ctenoid scales of the Pempheridae, considered a sister group of the Apgogonidae by some authors, follow the same ontogenetic pattern. Transforming ctenoid scales are the ancestral scale type in the Apogonidae, making their spinoid and cycloid scales a secondary loss or reversal. Though sharing the transforming ctenoid scale type with the apogonids, the ontogeny of this scale type in several scorpionfishes (Scorpaenidae) does not have a spinoid phase. Recent molecular studies indicate that gobiids are related to apogonids, but the goby species examined in this study have peripheral ctenoid scales that lacked a spinoid phase in their ontogeny. The observations made in this study suggest that the peripheral ctenoid scale, the whole ctenoid scale and the crenate scale found in percomorph fishes were derived from a transforming ctenoid scale. Scale morphology and ontogeny could provide useful characters for resolving relationships between percomorph families. 

Electron microscopy of nocardial cell division

1978 ◽  
Vol 36 (2) ◽  
pp. 398-399
Author(s):  
Janet Hearn Woodward
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
Potato Dextrose Agar ◽  
Transmission Electron ◽  
Noble Agar ◽  
Scanning Electron ◽  
Made In

Nocardia polychromogenes is an aerobic, gram (+), non-motile, partially acid-fast actinomycete with primary mycelia that fragment into bacillary and coccoid elements.For scanning electron microscopy, N. polychromogenes culture strain Waksman 3409-A was grown on Potato Dextrose Agar at 25 C for 12-72 h. Five mm2 sections of the colonies, including portions of the interior and perimeter were fixed by exposure to osmium fumes for 16-24 h and air dried for 2 h. Specimens, mounted on stubs and sputter coated with gold, were viewed in a Cambridge Stereoscan Mark II scanning electron microscope. For transmission electron microscopy, the organism was grown on Potato Dextrose Agar at 25 C for 12-32 h. Whole colonies, 1-2 mm in diameter, were fixed by exposure to osmium fumes for 24 h. After suspension in noble agar, cells taken from the periphery of the fixed colonies were stained with 0. 5% uranyl acetate made in acetate-veranol buffer.

Technic for the Study of Small, Soft-Bodied Aurelia with Scanning EM

1975 ◽  
Vol 33 ◽  
pp. 682-683
Author(s):  
Dorothy B. Spangenberg ◽  
William Kuenning
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Soft Tissues ◽  
Scanning Em ◽  
Scanning Electron ◽  
Made In ◽  
Sem Studies

Rapid advances have been made in the preservation and preparation of cells and soft tissues of vertebrates for scanning electron microscopy (SEM). These technics, however, only rarely have been applied to small soft-bodied invertebrates. When an effort was made to preserve intact metamorphosing Aurelia polyps (strobilae) for SEM studies, it became apparent that special methodology was needed. The following technic was developed:Aurelia polyps are induced to metamorphose with 1 x 10-5M thyroxine. Strobilae in the desired stage of metamorphosis are fixed in 2 changes of 3% gluteraldehyde for 1 hr. each. They are rinsed 2X in collidine buffer, pH 7.4 (based on). The organisms are dehydrated in grades of acetone from 30% to 100%. To avoid disturbing the strobilae, acetone is added to the dilute acetone every 10 min. to achieve concentrations of 50, 70, and 95%. The strobilae are rinsed 2X in 100% acetone for 5 and 10 minutes each.

Morphological and Chromatic Variation in Four Populations of Triatoma mexicana (Hemiptera: Reduviidae)

2020 ◽  
Author(s):  
Nancy Rivas ◽  
Victor Sánchez-Cordero ◽  
Alejandro D Camacho ◽  
Ricardo Alejandre-Aguilar
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphometric Analysis ◽  
Endemic Species ◽  
Geographical Origin ◽  
Morphological Characteristics ◽  
Adaptive Responses ◽  
And Behavior ◽  
Scanning Electron ◽  
Made In

Abstract Triatoma mexicana is an endemic species of Mexico and is distributed in the states of Hidalgo, Queretaro, Guanajuato, and San Luis Potosi, being naturally infected with Trypanosoma cruzi, which increases its importance in the region. The species description was made in 1848, but there are only a few studies on its morphology, biology, and behavior. The present manuscript shows the presence of morphological and chromatic variations among populations of T. mexicana from the states of Hidalgo (Valle del Mezquital and Meztitlan), Guanajuato and Queretaro. The study employed 136 specimens collected in four locations. Scanning electron microscopy was used to study the morphological characteristics of the head, pronotum, and scutellum; also, we measured the width of the abdomen and the total length in the specimens of each population. The morphometric analysis considered 19 variables in the previous structures. Significant differences were found in the dimensions of the head and pronotum, but not in the scutellum; there is clear discrimination among the four proposed populations. The chromatic patterns observed in the connexivum go from yellow to brown and show some significant differences related to geographical origin. The set of evaluated characters showed a higher degree of difference in the population of Guanajuato, clearly separating from the rest of the populations, indicating the possibility of a divergence process. The characteristics observed in the remaining populations could be adaptive responses to their habitat.

Revisions in the technique of epi-illumination light microscopy for the study of floral and vegetative apices

1980 ◽  
Vol 58 (23) ◽  
pp. 2491-2495 ◽  
Author(s):  
U. Posluszny ◽  
M. G. Scott ◽  
R. Sattler
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Serial Sectioning ◽  
Scanning Electron ◽  
Made In

Many improvements have been made in the technique of epi-illumination light microscopy. These modifications have resulted in micrographs of better resolution and subsequently in greater flexibility in the study of floral and vegetative apices. The preparation, mounting, microscopy, and photography of apices are discussed in detail. The correlation of this technique with others such as scanning electron microscopy and serial sectioning is discussed. The numerous applications of epi-illumination light microscopy are considered.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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