Interactions of Carbon Nanomaterials With Mammalian Cells

2006 ◽  
Vol 951 ◽  
Author(s):  
Pavan M. V. Raja ◽  
Jennifer Connolley ◽  
Lijie Ci ◽  
Gopal P. Ganesan ◽  
Pulickel M. Ajayan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Carbon Nanotube ◽  
Cell Growth ◽  
Growth Inhibition ◽  
Mammalian Cells ◽  
Carbon Nanomaterials ◽  
Cell Growth Inhibition ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon

ABSTRACTDespite their diverse application potential, carbon nanotubes (CNT) have adverse effects in vitro, and in vivo. Previous research has focused on the in vitro cytotoxic impact of CNT aggregates and associated nanoparticulate impurities. In this study, we compared the single-walled carbon nanotube (SWNT) aggregates, and their associated finely dispersed, non-aggregated carbon nanomaterials on rat aortic smooth muscle cells (SMC), through filtration of the aggregates from the CNT-treated cell culture media. In general, our research shows that the removal of single-walled carbon nanotube (SWNT) aggregates from cell culture test media inhibited the growth in SMC to a lower extent than the corresponding unfiltered media at pre-filtered SWNT dosages below 0.10 mg/ml. We also found suspended nanoparticles (likely amorphous and graphitic carbon associated with the SWNT) and a small quantity of SWNT in the filtered media may have contributed to the observed cell growth inhibition by the filtered media. In addition, we compared the effect of SWNT, a nano-sized material, with activated carbon (AC), a nanoporous, microparticulate material, on SMC growth. AC (0.1 mg/ml) was found to be less inhibitory to SMC growth than the SWNT aggregates and suspended matter (0.1 mg/ml), potentially implying an inverse proportionality between carbon nanomaterial size regimes and cell growth inhibition.

scholarly journals Effect of β-carotene-rich tomato lycopene β-cyclase (tlcy-b) on cell growth inhibition in HT-29 colon adenocarcinoma cells

2008 ◽  
Vol 102 (2) ◽  
pp. 207-214 ◽  
Author(s):  
Paola Palozza ◽  
Diana Bellovino ◽  
Rossella Simone ◽  
Alma Boninsegna ◽  
Francesco Cellini ◽  
...  
Keyword(s):  
Cell Growth ◽  
Growth Inhibition ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Cell Growth Inhibition ◽  
Adenocarcinoma Cells ◽  
Ht 29 ◽  
Colon Adenocarcinoma Cells ◽  
Β Carotene

Lycopene β-cyclase (tlcy-b) tomatoes, obtained by modulating carotenogenesis via genetic engineering, contain a large amount of β-carotene, as clearly visible by their intense orange colour. In the present study we have subjected tlcy-b tomatoes to an in vitro simulated digestion and analysed the effects of digestate on cell proliferation. To this aim we used HT-29 human colon adenocarcinoma cells, grown in monolayers, as a model. Digested tomatoes were diluted (20 ml, 50 ml and 100 ml/l) in culture medium and added to the cells for different incubation times (24 h, 48 h and 72 h). Inhibition of cell growth by tomato digestate was dose-dependent and resulted from an arrest of cell cycle progression at the G0/G1 and G2/M phase and by apoptosis induction. A down-regulation of cyclin D1, Bcl-2 and Bcl-xl expression was observed. We also found that heat treatment of samples before digestion enhanced β-carotene release and therefore cell growth inhibition. To induce with purified β-carotene solubilised in tetrahydrofuran the same cell growth inhibition obtained with the tomato digestate, a higher amount of the carotenoid was necessary, suggesting that β-carotene micellarised during digestion is utilised more efficiently by the cells, but also that other tomato molecules, reasonably made available during digestion, may be present and cooperate with β-carotene in promoting cell growth arrest.

Effects of O2 and N2/H2 plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds

2011 ◽  
Vol 257 (20) ◽  
pp. 8535-8541 ◽  
Author(s):  
Ok Ja Yoon ◽  
Hyun Jung Lee ◽  
Yeong Mi Jang ◽  
Hyun Woo Kim ◽  
Won Bok Lee ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Cell Growth ◽  
Neuronal Cell ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Plasma Treatments

Amine-functionalized Single-walled Carbon Nanotube/Polycaprolactone Electrospun Scaffold for Bone Tissue Engineering: in vitro Study

2019 ◽  
Vol 20 (9) ◽  
pp. 1869-1882 ◽  
Author(s):  
Hadi Tohidlou ◽  
Seyedeh Sara Shafiei ◽  
Shahsanam Abbasi ◽  
Mitra Asadi-Eydivand ◽  
Mehrnoosh Fathi-Roudsari
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Vitro Study ◽  
Electrospun Scaffold ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Amine Functionalized

A Facile Synthesis of 9-Deaza Analogue of Olomoucine

2003 ◽  
Vol 68 (4) ◽  
pp. 779-791 ◽  
Author(s):  
Petr Čapek ◽  
Miroslav Otmar ◽  
Milena Masojídková ◽  
Ivan Votruba ◽  
Antonín Holý
Keyword(s):  
Cell Growth ◽  
Growth Inhibition ◽  
Cell Lines ◽  
Alkaline Solution ◽  
Catalytic Hydrogenation ◽  
Cell Growth Inhibition ◽  
Facile Synthesis ◽  
Hydroxymethyl Group ◽  
Chloro Derivative

Heating of 6-(benzylamino)-2-chloro-9-deazapurine (3) with ethanolamine afforded 6-(benzylamino)-2-[(2-hydroxyethyl)amino]-9-deazapurine (8). Its treatment with formaldehyde in alkaline solution, after protection of the OH group with DMTr, led to hydroxymethylation at position 9. Conversion of the hydroxymethyl group to methyl was performed by catalytic hydrogenation under simultaneous deprotection, which resulted in the formation of the 9-deaza analogue 1 of olomoucine. Compound 1 does not exhibit any significant in vitro cell growth inhibition of CCRF-CEM, HeLa and L-1210 cell lines. Cytostatic activity was found in 6-(benzylamino)-9-deazapurine (2) and its 2-chloro derivative 3 in CCRF-CEM cells with IC50 13.3 and 15.8 μM, respectively.

In vitro nanotoxicity of single-walled carbon nanotube–dendrimer nanocomplexes against murine myoblast cells

2013 ◽  
Vol 219 (1) ◽  
pp. 18-25 ◽  
Author(s):  
J. Cancino ◽  
I.M.M. Paino ◽  
K.C. Micocci ◽  
H.S. Selistre-de-Araujo ◽  
V. Zucolotto
Keyword(s):  
Carbon Nanotube ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Myoblast Cells

scholarly journals Mechanism of In vitro Pancreatic Cancer Cell Growth Inhibition by Melanoma Differentiation–Associated Gene-7/Interleukin-24 and Perillyl Alcohol

2008 ◽  
Vol 68 (18) ◽  
pp. 7439-7447 ◽  
Author(s):  
Irina V. Lebedeva ◽  
Zhao-zhong Su ◽  
Nichollaq Vozhilla ◽  
Lejuan Chatman ◽  
Devanand Sarkar ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cell Growth ◽  
Growth Inhibition ◽  
Cancer Cell ◽  
Pancreatic Cancer Cell ◽  
Perillyl Alcohol ◽  
Cell Growth Inhibition ◽  
Cancer Cell Growth

Platinum(II) Complexes with Antitumoral/Antiviral Aromatic Heterocycles:  Effect of Glutathione upon in Vitro Cell Growth Inhibition

2005 ◽  
Vol 48 (9) ◽  
pp. 3364-3371 ◽  
Author(s):  
Paride Papadia ◽  
Nicola Margiotta ◽  
Alberta Bergamo ◽  
Gianni Sava ◽  
Giovanni Natile
Keyword(s):  
Cell Growth ◽  
Growth Inhibition ◽  
Cell Growth Inhibition ◽  
Aromatic Heterocycles

scholarly journals In Vitro Tumor Cell Growth Inhibition Induced by Lophocereus marginatus (DC.) S. Arias and Terrazas Endophytic Fungi Extracts

2021 ◽  
Vol 18 (18) ◽  
pp. 9917
Author(s):  
Jesica M. Ramírez-Villalobos ◽  
César I. Romo-Sáenz ◽  
Karla S. Morán-Santibañez ◽  
Patricia Tamez-Guerra ◽  
Ramiro Quintanilla-Licea ◽  
...  
Keyword(s):  
Cell Growth ◽  
Tumor Cell ◽  
Growth Inhibition ◽  
Endophytic Fungi ◽  
Tumor Cell Growth ◽  
Cell Growth Inhibition ◽  
Antitumor Effects ◽  
Ht 29 ◽  
Mcf 7

Endophytic fungi have become potential sources of antitumor agents, particularly against antineoplastic-resistant cancer cells, with marginal or nil adverse effects for the oncological patient. Endophytic fungi were isolated from stems of the Lophocereus marginatus cactus, commonly found in Mexico. Methanol extracts were then obtained from fungus liquid cultures and their effects on tumor cell growth against murine lymphoma (L5178Y-R), human colorectal adenocarcinoma (HT-29), and human breast cancer (MCF-7) cells were evaluated at concentrations ranging from 31 µg/mL to 250 µg/mL via the colorimetric 3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide reduction assay, using monkey kidney epithelial (MA-104) and human peripheral mononuclear (PBMC) cells as controls. Furthermore, we obtained the IC50 and the selectivity index (SI) was calculated from the IC50 ratio of normal and tumor cells. In addition, molecular identification of fungi showing cytotoxic activity was determined, using internal transcribed spacer molecular markers. PME-H001, PME-H002, PME-H005, PME-H007, and PME-H008 filamentous fungus strain extracts showed significant (p < 0.05) tumor cell growth inhibition. In particular, they significantly (p < 0.05) inhibited L5178Y-R cell growth, whereas the least susceptible cell line was HT-29. The endophytic strain PME-H008 of Cladosporium sp. caused the highest growth inhibition percentage against L5178Y-R and HT-29 cells with 96.6% (p < 0.01) and 42.5% (p < 0.05) respectively, and the highest SIs against L5178Y-R cells with 2.4 and 2.9 for MA-104 and PBMCs, respectively, whereas the PME-H005 extract showed SIs of 2.77 and 1.5 against MCF-7 and L5178Y-R cells, respectively, as compared with PBMCs. In addition, the endophytic strain PME-H007 of Metarhizium anisopliae caused the highest percentage of growth inhibition (p < 0.01) against MCF-7 cells with 55.8% at 250 µg/mL. We demonstrated in vitro antitumor effects of L. marginatus endophytic fungi. Further research will involve the isolation and in vivo testing of bioactive compounds.

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