Enhanced Human Bone Marrow Mesenchymal Stem Cell Chondrogenic Differentiation on Cold Atmospheric Plasma Modified Cartilage Scaffold

2014 ◽  
Vol 1723 ◽  
Author(s):  
Wei Zhu ◽  
Michael Keidar ◽  
Lijie Grace Zhang
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Chondrogenic Differentiation ◽  
Transforming Growth Factor ◽  
Human Bone ◽  
Atmospheric Plasma ◽  
Human Bone Marrow ◽  
Self Healing ◽  
Cold Atmospheric Plasma ◽  
Elisa Testing

ABSTRACTArticular cartilage is prone to degeneration and possesses extremely poor self-healing capacity due to its low cell density and absence of blood vessels. It has extensively reported tissue engineered scaffold can be a promising approach for cartilage repair. However, there still remains an inherent lack of desirable scaffolds that stimulate cartilage regrowth with appropriate functional properties. Therefore, in this study, we develop a biomimetic cartilage substitute comprising of electrospun polycaprolactone (PCL) with cold atmospheric plasma (CAP) modified cell favorable surface and sustained bioactive factor (bovine serum albumin (BSA) or transforming growth factor beta 1 (TGF-β1)) incorporated microspheres inside for improving stem cell chondrogenesis and cartilage regeneration. Scanning electron microscopy (SEM) analysis showed the drug delivery spheres homogeneously distribution in the fibrous scaffold. Furthermore, CAP treatment renders the scaffold’s surface more hydrophilic and results in more specific vitronectin adsorption as illustrated by contact angle and ELISA testing. Our results showed that the CAP treated scaffold can greatly improve growth and chondrogenic differentiation (such as increased glycosaminoglycan (GAG) synthesis) of human bone marrow-derived mesenchymal stem cells (MSCs).

scholarly journals DNA Methylation-Based Regulation of Human Bone Marrow-Derived Mesenchymal Stem/Progenitor Cell Chondrogenic Differentiation

2019 ◽  
Vol 207 (3-4) ◽  
pp. 115-126 ◽  
Author(s):  
Yu Nomura ◽  
Emilio Satoshi Hara ◽  
Yuya Yoshioka ◽  
Há Thi Nguyen ◽  
Shuji Nosho ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stem Cell ◽  
Chondrogenic Differentiation ◽  
Stem Cell Differentiation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cartilage Tissue ◽  
Stem Cell Markers ◽  
Cell Markers

Stem cells have essential applications in in vitro tissue engineering or regenerative medicine. However, there is still a need to understand more deeply the mechanisms of stem cell differentiation and to optimize the methods to control stem cell function. In this study, we first investigated the activity of DNA methyltransferases (DNMTs) during chondrogenic differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (hBMSCs) and found that DNMT3A and DNMT3B were markedly upregulated during hBMSC chondrogenic differentiation. In an attempt to understand the effect of DNMT3A and DNMT3B on the chondrogenic differentiation of hBMSCs, we transiently transfected the cells with expression vectors for the two enzymes. Interestingly, DNMT3A overexpression strongly enhanced the chondrogenesis of hBMSCs, by increasing the gene expression of the mature chondrocyte marker, collagen type II, more than 200-fold. Analysis of the methylation condition in the cells revealed that DNMT3A and DNMT3B methylated the promoter sequence of early stem cell markers, NANOG and POU5F1(OCT-4). Conversely, the suppression of chondrogenic differentiation and the increase in stem cell markers of hBMSCs were obtained by chemical stimulation with the demethylating agent, 5-azacitidine. Loss-of-function assays with siRNAs targeting DNMT3A also significantly suppressed the chondrogenic differentiation of hBMSCs. Together, these results not only show the critical roles of DNMTs in regulating the chondrogenic differentiation of hBMSCs, but also suggest that manipulation of DNMT activity can be important tools to enhance the differentiation of hBMSCs towards chondrogenesis for potential application in cartilage tissue engineering or cartilage regeneration.

scholarly journals Enhanced human bone marrow mesenchymal stem cell chondrogenic differentiation in electrospun constructs with carbon nanomaterials

Carbon ◽  
2016 ◽  
Vol 97 ◽  
pp. 1-13 ◽  
Author(s):  
Benjamin Holmes ◽  
Xiuqi Fang ◽  
Annais Zarate ◽  
Michael Keidar ◽  
Lijie Grace Zhang
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Chondrogenic Differentiation ◽  
Carbon Nanomaterials ◽  
Human Bone ◽  
Human Bone Marrow

scholarly journals Exosome-transported circRNA_0001236 enhances chondrogenesis and suppress cartilage degradation via the miR-3677-3p/Sox9 axis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Guping Mao ◽  
Yiyang Xu ◽  
Dianbo Long ◽  
Hong Sun ◽  
Hongyi Li ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mouse Model ◽  
Chondrogenic Differentiation ◽  
Cartilage Degradation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Luciferase Reporter ◽  
Gene And Protein Expression

Abstract Objectives Aberrations in exosomal circular RNA (circRNA) expression have been identified in various human diseases. In this study, we investigated whether exosomal circRNAs could act as competing endogenous RNAs (ceRNAs) to regulate the pathological process of osteoarthritis (OA). This study aimed to elucidate the specific MSC-derived exosomal circRNAs responsible for MSC-mediated chondrogenic differentiation using human bone marrow-derived MSCs (hMSCs) and a destabilization of the medial meniscus (DMM) mouse model of OA. Methods Exosomal circRNA deep sequencing was performed to evaluate the expression of circRNAs in human bone marrow-derived MSCs (hMSCs) induced to undergo chondrogenesis from day 0 to day 21. The regulatory and functional roles of exosomal circRNA_0001236 were examined on day 21 after inducing chondrogenesis in hMSCs and were validated in vitro and in vivo. The downstream target of circRNA_0001236 was also explored in vitro and in vivo using bioinformatics analyses. A luciferase reporter assay was used to evaluate the interaction between circRNA_0001236 and miR-3677-3p as well as the target gene sex-determining region Y-box 9 (Sox9). The function and mechanism of exosomal circRNA_0001236 in OA were explored in the DMM mouse model. Results Upregulation of exosomal circRNA_0001236 enhanced the expression of Col2a1 and Sox9 but inhibited that of MMP13 in hMSCs induced to undergo chondrogenesis. Moreover, circRNA_0001236 acted as an miR-3677-3p sponge and functioned in human chondrocytes via targeting miR-3677-3p and Sox9. Intra-articular injection of exosomal circRNA_0001236 attenuated OA in the DMM mouse model. Conclusions Our results reveal an important role for a novel exosomal circRNA_0001236 in chondrogenic differentiation. Overexpression of exosomal circRNA_0001236 promoted cartilage-specific gene and protein expression through the miR-3677-3p/Sox9 axis. Thus, circRNA_0001236-overexpressing exosomes may alleviate cartilage degradation, suppressing OA progression and enhancing cartilage repair. Our findings provide a potentially effective therapeutic strategy for treating OA.

Chemical interaction of polyphosphoric acid with titanium and its effect on human bone marrow derived mesenchymal stem cell behavior

2007 ◽  
Vol 82A (1) ◽  
pp. 195-200 ◽  
Author(s):  
Kenji Maekawa ◽  
Yasuhiro Yoshida ◽  
Atsushi Mine ◽  
Takuo Fujisawa ◽  
Bart Van Meerbeek ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Polyphosphoric Acid ◽  
Chemical Interaction ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Behavior
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