Cartilage: Biomimetic Study of the Extracellular Matrix

2014 ◽  
Vol 1622 ◽  
pp. 61-68
Author(s):  
Chinedu I. Anyaeji ◽  
Peter J. Basser ◽  
Ferenc Horkay
Keyword(s):  
Extracellular Matrix ◽  
Biological Tissue ◽  
Vinyl Alcohol ◽  
Biological Function ◽  
External Loading ◽  
Poly Vinyl Alcohol ◽  
Swelling Properties ◽  
Macroscopic Behavior ◽  
Cartilage Extracellular Matrix ◽  
Compressive Resistance

ABSTRACTCartilage is a complex biological tissue that exhibits gel-like behavior. Its primary biological function is providing compressive resistance to external loading and nearly frictionless lubrication of joints. In this study, we model cartilage extracellular matrix using a biomimetic system. We demonstrate that poly(vinyl) alcohol (PVA) hydrogels are robust biomaterials exhibiting mechanical and swelling properties similar to that of cartilage extracellular matrix. A comparison is made between the macroscopic behavior of PVA gels and literature data reported for cartilage.

scholarly journals Bioprinting Via a Dual-Gel Bioink Based on Poly(Vinyl Alcohol) and Solubilized Extracellular Matrix towards Cartilage Engineering

2021 ◽  
Vol 22 (8) ◽  
pp. 3901
Author(s):  
Mohsen Setayeshmehr ◽  
Shahzad Hafeez ◽  
Clemens van Blitterswijk ◽  
Lorenzo Moroni ◽  
Carlos Mota ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Viability ◽  
Vinyl Alcohol ◽  
Compression Modulus ◽  
Synthetic Polymers ◽  
Poly Vinyl Alcohol ◽  
Cross Linking ◽  
Light Curing ◽  
Shape Retention ◽  
Amine Groups

Various hydrogel systems have been developed as biomaterial inks for bioprinting, including natural and synthetic polymers. However, the available biomaterial inks, which allow printability, cell viability, and user-defined customization, remains limited. Incorporation of biological extracellular matrix materials into tunable synthetic polymers can merge the benefits of both systems towards versatile materials for biofabrication. The aim of this study was to develop novel, cell compatible dual-component biomaterial inks and bioinks based on poly(vinyl alcohol) (PVA) and solubilized decellularized cartilage matrix (SDCM) hydrogels that can be utilized for cartilage bioprinting. In a first approach, PVA was modified with amine groups (PVA-A), and mixed with SDCM. The printability of the PVA-A/SDCM formulations cross-linked by genipin was evaluated. On the second approach, the PVA was functionalized with cis-5-norbornene-endo-2,3-dicarboxylic anhydride (PVA-Nb) to allow an ultrafast light-curing thiol-ene cross-linking. Comprehensive experiments were conducted to evaluate the influence of the SDCM ratio in mechanical properties, water uptake, swelling, cell viability, and printability of the PVA-based formulations. The studies performed with the PVA-A/SDCM formulations cross-linked by genipin showed printability, but poor shape retention due to slow cross-linking kinetics. On the other hand, the PVA-Nb/SDCM showed good printability. The results showed that incorporation of SDCM into PVA-Nb reduces the compression modulus, enhance cell viability, and bioprintability and modulate the swelling ratio of the resulted hydrogels. Results indicated that PVA-Nb hydrogels containing SDCM could be considered as versatile bioinks for cartilage bioprinting.

Synthesis of Poly(vinyl alcohol)/Chitosan/Titanium Oxide Beads

2013 ◽  
Vol 65 (4) ◽  
Author(s):  
Syazwan Liyana Sulaiman ◽  
Zawati Harun ◽  
Sufizar Ahmad ◽  
Hariati Taib
Keyword(s):  
Wastewater Treatment ◽  
Titanium Oxide ◽  
Alkaline Solution ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Distilled Water ◽  
Swelling Properties ◽  
Promising Alternative ◽  
Porous Beads ◽  
Mean Time

The application of photocatalytic beads in wastewater treatment application has gained attention of many researchers. In this study, the synthesised poly(vinyl alcohol)/chitosan/Titanium oxide (PVA/CS/TiO2) beads has been considered as a promising alternative to conventional means of wastewater treatment. The present study has successfully synthesised PVA/CS/TiO2 beads through simple dropwise method in which varied concentration of CS from 1.0 g to 2.5 g. Morphological analysis of the synthesised PVA/CS/TiO2 beads investigated by the FE-SEM technique revealed that porous beads were obtained regardless of the varied CS concentration content. Furthermore, solubility and swelling properties investigation of the beads were also performed within the prescribed time of 24h in three different medium which were acidic, distilled water and alkaline solution. Result of solubility test proved that PVA/CS/TiO2 beads were insoluble only in distilled water and alkaline solution. However, the beads were found to dissolve in acidic solution due to the CS content. Meanwhile, the swelling test revealed that increased concentration of CS leads to swelling of beads in distilled water and alkaline solution. Results revealed that beads with the highest CS composition which is 2.5 g recorded 82.6% and 118.4% of swelling in distilled water and alkaline solution, respectively. In the mean time, beads with the lowest CS composition which is 1.0 g only swell for 65.8% and 93.3% in distilled water and alkaline solution, respectively. As a conclusion, the synthesised beads in this study is feasible to be applied in natural to alkaline environment.

Electrospun nanostructured chitosan–poly(vinyl alcohol) scaffolds: a biomimetic extracellular matrix as dermal substitute

2012 ◽  
Vol 7 (4) ◽  
pp. 045005 ◽  
Author(s):  
Dhakshinamoorthy Sundaramurthi ◽  
Kirthanashri Srinivasan Vasanthan ◽  
Purushothaman Kuppan ◽  
Uma Maheswari Krishnan ◽  
Swaminathan Sethuraman
Keyword(s):  
Extracellular Matrix ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Dermal Substitute

Albumin and urea production by hepatocytes cultured on extracellular matrix proteins-conjugated poly (vinyl alcohol) membranes

2005 ◽  
Vol 16 (7) ◽  
pp. 847-860 ◽  
Author(s):  
Akon Higuchi ◽  
Marie Kurihara ◽  
Kenichi Kobayashi ◽  
Chong Su Cho ◽  
Toshihiro Akaike ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Vinyl Alcohol ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Poly Vinyl Alcohol ◽  
Urea Production

scholarly journals Optimized swelling properties of hydrogels based on poly(vinyl alcohol)-carrageenan

2020 ◽  
Author(s):  
D. Rahmi ◽  
S. Paramadina ◽  
M. Anjelika ◽  
R. Widjajanti
Keyword(s):  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Swelling Properties

scholarly journals Synthesis and Properties of pH-, Thermo-, and Salt-Sensitive Modified Poly(aspartic acid)/Poly(vinyl alcohol) IPN Hydrogel and Its Drug Controlled Release

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Jingqiong Lu ◽  
Yinhui Li ◽  
Deng Hu ◽  
Xiaoling Chen ◽  
Yongmei Liu ◽  
...  
Keyword(s):  
Controlled Release ◽  
Aspartic Acid ◽  
Vinyl Alcohol ◽  
Polymer Network ◽  
Interpenetrating Polymer Network ◽  
Simulated Gastric Fluid ◽  
Poly Vinyl Alcohol ◽  
Simulated Intestinal Fluid ◽  
Swelling Properties ◽  
Drug Controlled Release

Modified poly(aspartic acid)/poly(vinyl alcohol) interpenetrating polymer network (KPAsp/PVA IPN) hydrogel for drug controlled release was synthesized by a simple one-step method in aqueous system using poly(aspartic acid) grafting 3-aminopropyltriethoxysilane (KH-550) and poly(vinyl alcohol) (PVA) as materials. The hydrogel surface morphology and composition were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The thermal stability was analyzed by thermogravimetric analysis (TGA). The swelling properties and pH, temperature, and salt sensitivities of KPAsp, KPAsp/PVA semi-interpenetrating polymer network (semi-IPN), and KPAsp/PVA IPN hydrogels were also investigated. All of the three hydrogels showed ampholytic pH-responsive properties, and swelling behavior was also extremely sensitive to the temperature, ionic strength, and cationic species. Finally, the drug controlled release properties of the three hydrogels were evaluated and results indicated that three hydrogels could control drug release by external surroundings stimuli. The drug controlled release properties of KPAsp/PVA IPN hydrogel are the most outstanding, and the correlative measured release profiles of salicylic acid at 37°C were 32.6 wt% at pH = 1.2 (simulated gastric fluid) and 62.5 wt% at pH = 7.4 (simulated intestinal fluid), respectively. These results indicated that KPAsp/PVA IPN hydrogels are a promising carrier system for controlled drug delivery.

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