Control of Cell Adhesion and Detachment on Temperature-Responsive Block Copolymer Langmuir Films

2014 ◽  
Vol 1621 ◽  
pp. 101-106 ◽  
Author(s):  
Morito Sakuma ◽  
Yoshikazu Kumashiro ◽  
Masamichi Nakayama ◽  
Nobuyuki Tanaka ◽  
Umemura Kazuo ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Block Copolymer ◽  
Raft Polymerization ◽  
Langmuir Films ◽  
Chemical Compositions ◽  
Langmuir Film ◽  
Atomic Force ◽  
Responsive Surfaces ◽  
Modified Surface ◽  
Response To Temperature

ABSTRACTThis study used Langmuir-Schaefer (LS) method to produce thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) modified surface. Block copolymer composed of polystyrene (PSt) and PIPAAm was synthesized by RAFT polymerization. PSt-block-PIPAAm (St-IP) with various chemical compositions was dropped on an air-water interface and formed Langmuir film by compression. Then, the Langmuir film changing a density was transferred on a hydrophobic modified glass substrate to produce St-IP transferred surface (St-IP LS surface). From the observation of atomic force microscope images, a nanostructure was observed on the transference of Langmuir films. Cell adhesion and detachment were also evaluated on the LS surfaces in response to temperature. Cell adhesion on LS surfaces at 37 °C was controlled by changing the chemical compositions and densities. After reducing temperature to 20 °C, adhering cells rapidly detached themselves with lower Am and higher composition of PIPAAm. Our method should be proved novel insights for investigating cell adhesion and detachment on thermo-responsive surfaces.

scholarly journals Dispersion of Few-Layer Black Phosphorus in Binary Polymer Blend and Block Copolymer Matrices

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1996
Author(s):  
Serena Coiai ◽  
Elisa Passaglia ◽  
Simone Pinna ◽  
Stefano Legnaioli ◽  
Silvia Borsacchi ◽  
...  
Keyword(s):  
Thin Films ◽  
Raman Spectroscopy ◽  
Block Copolymer ◽  
Raft Polymerization ◽  
Black Phosphorus ◽  
Force Microscopy ◽  
Atomic Force ◽  
Binary Polymer ◽  
Reversible Addition ◽  
Copolymer Matrices

Exfoliated black phosphorus (bP) embedded into a polymer is preserved from oxidation, is stable to air, light, and humidity, and can be further processed into devices without degrading its properties. Most of the examples of exfoliated bP/polymer composites involve a single polymer matrix. Herein, we report the preparation of biphasic polystyrene/poly(methyl methacrylate) (50/50 wt.%) composites containing few-layer black phosphorus (fl-bP) (0.6–1 wt.%) produced by sonicated-assisted liquid-phase exfoliation. Micro-Raman spectroscopy confirmed the integrity of fl-bP, while scanning electron microscopy evidenced the influence of fl-bP into the coalescence of polymeric phases. Furthermore, the topography of thin films analyzed by atomic force microscopy confirmed the effect of fl-bP into the PS dewetting, and the selective PS etching of thin films revealed the presence of fl-bP flakes. Finally, a block copolymer/fl-bP composite (1.2 wt.%) was prepared via in situ reversible addition–fragmentation chain transfer (RAFT) polymerization by sonication-assisted exfoliation of bP into styrene. For this sample, 31P solid-state NMR and Raman spectroscopy confirmed an excellent preservation of bP structure.

TUNING PROPERTIES AND MORPHOLOGY IN HIGH VINYL CONTENT SBS BLOCK COPOLYMER, A THERMOPLASTIC ELASTOMER VIA THIOL-ENE MODIFICATION

2017 ◽  
Vol 90 (3) ◽  
pp. 550-561 ◽  
Author(s):  
Prithwiraj Mandal ◽  
Siva Ponnupandian ◽  
Soumyadip Choudhury ◽  
Nikhil K. Singha
Keyword(s):  
Block Copolymer ◽  
Thermoplastic Elastomer ◽  
Atomic Force Microscopy Analysis ◽  
Ene Reaction ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microscopy Analysis ◽  
Styrene Butadiene Styrene ◽  
Styrene Butadiene ◽  
Butadiene Styrene

ABSTRACT Thiol-ene modification of high vinyl content thermoplastic elastomeric styrene butadiene styrene (SBS) block copolymer (BCP) was carried out using different thiolating agents in toluene at 70 °C. 1H NMR analysis confirmed the participation of vinyl double bond in the thiol-ene modification reaction of SBS. Surface morphology of the block copolymers evaluated by atomic force microscopy analysis showed higher roughness after the thiol-ene reaction. The thiol-modified SBS block copolymer showed better adhesion strength and oil resistance properties than the pristine SBS.

scholarly journals Evaluation of Cell Adhesion Force with Atomic Force Microscope

2000 ◽  
Vol 40 (supplement) ◽  
pp. S80
Author(s):  
H. Kim ◽  
T. Osada ◽  
A. Ikai
Keyword(s):  
Cell Adhesion ◽  
Atomic Force Microscope ◽  
Adhesion Force ◽  
Atomic Force

One-pot synthesis of PLA-b-PHEA via sequential ROP and RAFT polymerizations

2017 ◽  
Vol 8 (39) ◽  
pp. 6086-6098 ◽  
Author(s):  
Ilknur Yildirim ◽  
Pelin Sungur ◽  
Anna C. Crecelius-Vitz ◽  
Turgay Yildirim ◽  
Diana Kalden ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Ring Opening ◽  
Raft Polymerization ◽  
Ring Opening Polymerization ◽  
One Pot ◽  
One Pot Synthesis

A block copolymer library of polylactide and poly(2-hydroxyethyl acrylate) was prepared via sequential ring opening polymerization and RAFT polymerization in a one-pot approach.

scholarly journals Synthesis of amphiphilic ABA triblock oligomer via ATRP and its surface properties

2017 ◽  
Vol 95 (5) ◽  
pp. 605-611 ◽  
Author(s):  
Lei Wang ◽  
Shaoqing Wen ◽  
Zhanxiong Li
Keyword(s):  
Photoelectron Spectroscopy ◽  
Microphase Separation ◽  
Gel Permeation Chromatography ◽  
Contact Angles ◽  
Chemical Compositions ◽  
Water Contact ◽  
Force Microscopy ◽  
Atomic Force ◽  
Poly Ethylene ◽  
Magnetic Resonances

A series of novel amphiphilic ABA-type poly(tridecafluorooctylacrylate)-poly(ethylene glycol)-poly(tridecafluorooctylacrylate) (henceforth referred to as p-TDFA-PEG-p-TDFA) triblock oligomers were successfully synthesized via atom transfer radical polymerization (ATRP) using well-defined Br-PEG-Br as macroinitiator and copper as catalyst. The block oligomers were characterized by Fourier transform infrared (FTIR) spectroscopy and 1H and 19F nuclear magnetic resonances (NMR). Gel permeation chromatography (GPC) showed that the block oligomers have been obtained with narrow molecular weight distributions of 1.22–1.33. X-ray photoelectron spectroscopy (XPS) was carried out to confirm the attachment of p-TDFA-PEG-p-TDFA onto the silicon substrate, together with the chemical compositions of p-TDFA-PEG-p-TDFA. The wetabilities of the oligomer films were measured by water contact angles (CAs). Water CAs of p-TDFA-PEG-p-TDFA film were measured and their morphologies were tested by atomic force microscopy (AFM). The result showed that the CAs of the oligomer films, which possess fluoroalkyl groups assembled on the outer surface, increase after heating due to the migration of fluoroalkyl groups and the resulted microphase separation of the p-TDFA-PEG-p-TDFA.

Mixed polymeric micelles as a multifunctional visual thermosensor for the rapid analysis of mixed metal ions with Al3+ and Fe3+

2018 ◽  
Vol 42 (15) ◽  
pp. 12853-12864 ◽  
Author(s):  
Juan Han ◽  
Yunfeng Cai ◽  
Yun Wang ◽  
Xiaohui Dai ◽  
Lei Wang ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Metal Ions ◽  
Chain Transfer ◽  
Polymeric Micelles ◽  
Raft Polymerization ◽  
Rapid Analysis ◽  
Mixed Metal ◽  
Reversible Addition ◽  
Mixed Polymeric Micelles

A novel type of responsive mixed double hydrophilic block copolymer (DHBC)-based multifunctional visual thermosensor for the detection of Al3+ and Fe3+ was designed and synthesized based on reversible addition fragmentation chain transfer (RAFT) polymerization.

RAFT polymerization of a RGD peptide-based methacrylamide monomer for cell adhesion

2018 ◽  
Vol 9 (14) ◽  
pp. 1780-1786 ◽  
Author(s):  
Chao Chen ◽  
San H. Thang
Keyword(s):  
Cell Adhesion ◽  
Raft Polymerization ◽  
Rgd Peptide ◽  
Robust Method ◽  
Support Cell

The present study provides a robust method for the preparation of RGD peptide-based polymers that has important implications in the synthesized biomaterials that support cell adhesion.

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