Electron Spin Resonance (ESR) Observation of Radicals on Biological Organism Interacted with Plasmas

2012 ◽  
Vol 1469 ◽  
Author(s):  
Kenji Ishikawa ◽  
Hiroko Moriyama ◽  
Kazuhiro Tamiya ◽  
Hiroshi Hashizume ◽  
Takayuki Ohta ◽  
...  
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Reaction Mechanism ◽  
Electron Spin ◽  
Fungal Spores ◽  
Plasma Discharge ◽  
Oxygen Species ◽  
Spin Resonance ◽  
G Value

ABSTRACTWe report the kinetic analysis of radicals on fungal spores of Penicillium digitatum interacted with charged-neutral oxygen species (O*) generated plasma discharge using real time in situ electron spin resonance (ESR) measurements. The ESR signal from the spores was observed at a g-value of around 2.004 with a line width of approximately 5G. We have successfully obtained information regarding the reaction mechanism with free radicals and realtime in situ ESR has proven to be a useful method to elucidate plasma-induced surface reactions on biological specimens

scholarly journals Real-time in situ electron spin resonance measurements on fungal spores of Penicillium digitatum during exposure of oxygen plasmas

2012 ◽  
Vol 101 (1) ◽  
pp. 013704 ◽  
Author(s):  
Kenji Ishikawa ◽  
Hiroko Mizuno ◽  
Hiromasa Tanaka ◽  
Kazuhiro Tamiya ◽  
Hiroshi Hashizume ◽  
...  
Keyword(s):  
Electron Spin Resonance ◽  
Real Time ◽  
Electron Spin ◽  
Fungal Spores ◽  
Penicillium Digitatum ◽  
Spin Resonance

Free radicals in the reaction between t-butyl hydroperoxide and titanous ion: Some observations by electron spin resonance spectrometry

1965 ◽  
Vol 18 (8) ◽  
pp. 1177 ◽  
Author(s):  
MFR Mulcahy ◽  
JR Steven ◽  
JC Ward
Keyword(s):  
Aqueous Solution ◽  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Peroxy Radicals ◽  
Methyl Radicals ◽  
Methyl Radical ◽  
Butyl Hydroperoxide ◽  
Spin Resonance ◽  
G Value

The reaction between t-butyl hydroperoxide and titanous ion in aqueous solution produces free methyl radicals detectable by electron spin resonance spectrometry (Dixon and Norman). However, the presence of titanous ion in concentrations greater than 0.01M broadens the spectrum of the methyl radical, causing it effectively to disappear at titanous concentrations greater than 0.1M. At hydroperoxide concentrations above 0.25M t-butyl peroxy radicals (identified by a strong single-line spectrum with g-value 2.0136) are produced by the reaction ���������� R. + (CH3)3COOH → RH + (CH3)3COO. Their concentration reaches a maximum about 1 sec after the concentration of the methyl radicals has fallen to an undetectable value and their half-life (≈ 5 sec) is about ten times that of the methyl radicals.

scholarly journals Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-11
Author(s):  
Shinobu Ito ◽  
Tomohisa Mori ◽  
Hideko Kanazawa ◽  
Toshiko Sawaguchi
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Spin Trap ◽  
Detection Sensitivity ◽  
Mouse Tissue ◽  
Spin Adduct ◽  
Simple Method ◽  
Oxidation Stress ◽  
Spin Resonance

Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗in liver (y=113.8–40.7 log (day),R1=-0.779,R2=0.6,P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

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