Computer-assisted designing and biofabrication of 3-D hydrogel structures towards thick 3-D tissue engineering

2012 ◽  
Vol 1415 ◽  
Author(s):  
Makoto Nakamura ◽  
Kenichi Arai ◽  
Hideki Toda ◽  
Shintaroh Iwanaga ◽  
Kozo Ito ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Image Data ◽  
Honeycomb Structure ◽  
Digital Data ◽  
Computer Assisted ◽  
Alginate Hydrogel ◽  
Cell Printing ◽  
Custom Made ◽  
Direct Cell

ABSTRACTEngineering and manufacturing of thick and bio-functional tissue products is one of the big issues in tissue engineering. To produce such tissues, we need some innovative technologies, which enable us to build up thick, three-dimensional structures and to arrange multiple types of cells to make complicated tissue structures. Based on such considerations, we have developed a custom-made inkjet 3D bioprinter, which realized both of direct cell printing and 3D laminating printing with cells and hydrogel. Recently, it has been improved, and here we report recent progresses and our achievements with new version 3D bioprinter.Image based printing mode and active Z-axis control system were added. As a useful structure, an image of multi-honeycomb pattern was designed in computer and next it was copied and finally in total 100 image data were prepared. Using those digital data, 3D image of thick multi-honeycomb structure was reconstructed in computer, and then, laminating printing was carried out using our new version 3D bioprinter with alginate hydrogel. The new version printer showed good performance of 3D laminating printing and finally complicated 3D multi-honeycomb hydrogel structures could be successfully fabricated. It is indicated that fabrication of cell containing 3D structures based on the computer aided designs is feasible and that such biofabrication technologies must contribute to further innovative advancement of tissue engineering.

scholarly journals Advances in 3D Printing for Tissue Engineering

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3149
Author(s):  
Angelika Zaszczyńska ◽  
Maryla Moczulska-Heljak ◽  
Arkadiusz Gradys ◽  
Paweł Sajkiewicz
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
3D Printing ◽  
Complex Structure ◽  
Three Dimensional ◽  
Computer Assisted ◽  
Scaffold Fabrication ◽  
Manufacturing Techniques ◽  
Printing Techniques ◽  
State Of Art

Tissue engineering (TE) scaffolds have enormous significance for the possibility of regeneration of complex tissue structures or even whole organs. Three-dimensional (3D) printing techniques allow fabricating TE scaffolds, having an extremely complex structure, in a repeatable and precise manner. Moreover, they enable the easy application of computer-assisted methods to TE scaffold design. The latest additive manufacturing techniques open up opportunities not otherwise available. This study aimed to summarize the state-of-art field of 3D printing techniques in applications for tissue engineering with a focus on the latest advancements. The following topics are discussed: systematics of the available 3D printing techniques applied for TE scaffold fabrication; overview of 3D printable biomaterials and advancements in 3D-printing-assisted tissue engineering.

scholarly journals Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3522
Author(s):  
Su Jeong Lee ◽  
Jun Hee Lee ◽  
Jisun Park ◽  
Wan Doo Kim ◽  
Su A Park
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Research Groups ◽  
Porcine Skin ◽  
Alginate Hydrogel ◽  
Chemical Treatments ◽  
Soft Tissue Engineering

Recently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine skin powder (PSP) to determine rheological properties, biocompatibility, and extracellular matrix (ECM) formation in cells in PSP-ink after 3D printing. PSP was extracted without cells by mechanical, enzymatic, and chemical treatments of porcine dermis tissue. Our developed PSP-containing bio-ink showed enhanced printability and biocompatibility. To identify whether the bio-ink was printable, the viscosity of bio-ink and alginate hydrogel was analyzed with different concentration of PSP. As the PSP concentration increased, viscosity also increased. To assess the biocompatibility of the PSP-containing bio-ink, cells mixed with bio-ink printed structures were measured using a live/dead assay and WST-1 assay. Nearly no dead cells were observed in the structure containing 10 mg/mL PSP-ink, indicating that the amounts of PSP-ink used were nontoxic. In conclusion, the proposed skin dermis decellularized bio-ink is a candidate for 3D bioprinting.

Three-dimensional reconstruction based on images from spiral high-resolution computed tomography of the temporal bone: anatomy and clinical application

2005 ◽  
Vol 119 (9) ◽  
pp. 693-698 ◽  
Author(s):  
Beom-Cho Jun ◽  
Sun-Wha Song ◽  
Ju-Eun Cho ◽  
Chan-Soon Park ◽  
Dong-Hee Lee ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
3D Reconstruction ◽  
Temporal Bone ◽  
Three Dimensional ◽  
Image Data ◽  
Computer Assisted ◽  
Surface Rendering ◽  
High Resolution Computed Tomography

The aim of this study was to investigate the usefulness of a three-dimensional (3D) reconstruction of computed tomography (CT) images in determining the anatomy and topographic relationship between various important structures. Using 40 ears from 20 patients with various otological diseases, a 3D reconstruction based on the image data from spiral high-resolution CT was performed by segmentation, volume-rendering and surface-rendering algorithms on a personal computer. The 3D display of the middle and inner ear structures was demonstrated in detail. Computer-assisted measurements, many of which could not be easily measured in vivo, of the reconstructed structures provided accurate anatomic details that improved the surgeon’s understanding of spatial relationships. A 3D reconstruction of temporal bone CT might be useful for education and increasing understanding of the anatomical structures of the temporal bone. However, it will be necessary to confirm the correlation between the 3D reconstructed images and histological sections through a validation study.

scholarly journals Recent Cell Printing Systems for Tissue Engineering

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Hyeongjin Lee ◽  
YoungWon Koo ◽  
Miji Yeo ◽  
SuHon Kim ◽  
Geun Hyung Kim
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
3D Printing ◽  
Cell Damage ◽  
Three Dimensional ◽  
Human Tissues ◽  
Printing Process ◽  
Cell Printing ◽  
3D Space ◽  
Printing Ink

 Three-dimensional (3D) printing in tissue engineering has been studied for the bio mimicry of the structures of human tissues and organs. Now it is being applied to 3D cell printing, which can position cells and biomaterials, such as growth factors, at desired positions in the 3D space. However, there are some challenges of 3D cell printing, such as cell damage during the printing process and the inability to produce a porous 3D shape owing to the embedding of cells in the hydrogel-based printing ink, which should be biocompatible, biodegradable, and non-toxic, etc. Therefore, researchers have been studying ways to balance or enhance the post-print cell viability and the print-ability of 3D cell printing technologies by accommodating several mechanical, electrical, and chemical based systems. In this mini-review, several common 3D cell printing methods and their modified applications are introduced for overcoming deficiencies of the cell printing process.

Direct Freeform Fabrication Technique for Bio-Manufacturing of Pre-Seeded, Spatially Heterogeneous, Anatomically-Shaped Alginate Hydrogel Implants

2005 ◽  
Author(s):  
Daniel L. Cohen ◽  
Evan Malone ◽  
Hod Lipson ◽  
Lawrence J. Bonassar
Keyword(s):  
Tissue Engineering ◽  
Solid Freeform Fabrication ◽  
Crosslinking Agent ◽  
Three Dimensional ◽  
Patient Specific ◽  
Efficient Production ◽  
Alginate Hydrogel ◽  
Freeform Fabrication ◽  
Patient Specific Implants ◽  
Multiple Material

A major challenge in orthopaedic tissue engineering is the generation of cell-seeded implants with structures that mimic native tissue, both in terms of anatomic geometries and intratissue cell distributions. By combining the strengths of injection molding tissue engineering with those of Solid Freeform Fabrication (SFF), three-dimensional pre-seeded implants were fabricated without custom-tooling, enabling efficient production of patient-specific implants. The incorporation of SFF technology also enables the fabrication of geometrically complex, multiple-material implants with spatially heterogeneous cell distributions that could not otherwise be produced. Using a custom-built robotic SFF platform and gel deposition tools, alginate hydrogel was used with calcium sulfate as a crosslinking agent to produce pre-seeded living implants of arbitrary geometries. The process was determined to be sterile and viable at 94±5%. The GAG production was found to be about half that of a similarly molded samples. The compressive elastic modulus was determined to be 1.462±0.113 kPa.

Collagen-alginate as bioink for three-dimensional (3D) cell printing based cartilage tissue engineering

2018 ◽  
Vol 83 ◽  
pp. 195-201 ◽  
Author(s):  
Xingchen Yang ◽  
Zhenhui Lu ◽  
Huayu Wu ◽  
Wei Li ◽  
Li Zheng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Cell Printing

Biofabrication of controllable alginate hydrogel cell scaffolds based on bipolar electrochemistry

2021 ◽  
pp. 088391152110539
Author(s):  
Fei Xie ◽  
Changyue Li ◽  
Xiaoqing Hua ◽  
Li Ma
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Growth Model ◽  
Calcium Alginate ◽  
Three Dimensional ◽  
Alginate Hydrogel ◽  
Bipolar Electrodes ◽  
Cell Scaffolds ◽  
Alginate Hydrogels ◽  
Bipolar Electrochemistry

Bipolar electrochemistry successfully realized the electrodeposition of calcium alginate hydrogels in specific target areas in tissue engineering. However, the shape and quantity of three-dimensional cannot be accurately controlled. We presented a novel growth model for fabricating hydrogels based on bipolar electrochemical by patterned bipolar electrodes using photolithography. This work highlights pattern customization and quantitative control of hydrogels in cell culture platforms. Furthermore, alginate hydrogels with different heights can be controlled by adjusting the key parameters of the growth model. This strategy exhibits promising potential for cell-oriented scaffolds in tissue engineering.

MORPHOMUSEUM: AN ONLINE PLATFORM FOR PUBLICATION AND STORAGE OF VIRTUAL SPECIMENS

2016 ◽  
Vol 22 ◽  
pp. 183-195 ◽  
Author(s):  
Renaud Lebrun ◽  
Maëva J. Orliac
Keyword(s):  
Review Process ◽  
Scientific Journal ◽  
Three Dimensional ◽  
Digital Data ◽  
Morphological Data ◽  
Computer Assisted ◽  
Scientific Journals ◽  
Unique Identifier ◽  
Scientific Publications ◽  
And Storage

AbstractSince the early 1990s, methods for the acquisition of three-dimensional (3-D) data and computer-assisted techniques for the visualization of such data have grown increasingly popular among biologists, paleontologists, and paleoanthropologists. However, thus far no standardized repository for complex virtual models based on 3-D digital data of specimens has emerged, whereas the need for researchers to provide access to 3-D models of specimens as well as the pressure imposed on authors by scientific journals to make original 3-D morphological data publicly available have increased. MorphoMuseuM (M3) aims to fill this gap. M3 is both a peer-reviewed scientific journal (M3 Journal) and a virtual specimen repository (M3 Repository). All scientific articles and their associated 3-D models deposited in M3 go through a formal review process. Each published model is given a DOI and a unique identifier code, which should be cited by researchers using this model in their scientific publications. In this paper, we describe the place of M3 among other online repositories for 3-D data, and explain how the growing community of biologists working with 3-D data can benefit from using M3.

scholarly journals Tissue engineering and surgery: from translational studies to human trials

2017 ◽  
Vol 2 (4) ◽  
pp. 189-202 ◽  
Author(s):  
Jan Jeroen Vranckx ◽  
Margot Den Hondt
Keyword(s):  
Tissue Engineering ◽  
Wound Repair ◽  
Three Dimensional ◽  
Three Dimensional Printing ◽  
Engineering Sciences ◽  
Custom Made ◽  
Intrinsic Complexity ◽  
Dimensional Printing ◽  
Extensive Burn

AbstractTissue engineering was introduced as an innovative and promising field in the mid-1980s. The capacity of cells to migrate and proliferate in growth-inducing medium induced great expectancies on generating custom-shaped bioconstructs for tissue regeneration. Tissue engineering represents a unique multidisciplinary translational forum where the principles of biomaterial engineering, the molecular biology of cells and genes, and the clinical sciences of reconstruction would interact intensively through the combined efforts of scientists, engineers, and clinicians. The anticipated possibilities of cell engineering, matrix development, and growth factor therapies are extensive and would largely expand our clinical reconstructive armamentarium. Application of proangiogenic proteins may stimulate wound repair, restore avascular wound beds, or reverse hypoxia in flaps. Autologous cells procured from biopsies may generate an ‘autologous’ dermal and epidermal laminated cover on extensive burn wounds. Three-dimensional printing may generate ‘custom-made’ preshaped scaffolds – shaped as a nose, an ear, or a mandible – in which these cells can be seeded. The paucity of optimal donor tissues may be solved with off-the-shelf tissues using tissue engineering strategies. However, despite the expectations, the speed of translation of in vitro tissue engineering sciences into clinical reality is very slow due to the intrinsic complexity of human tissues. This review focuses on the transition from translational protocols towards current clinical applications of tissue engineering strategies in surgery.

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