Biotechnological Mineral Composites via Vaterite Precursors

2012 ◽  
Vol 1465 ◽  
Author(s):  
E. Weber ◽  
C. Guth ◽  
M. Eder ◽  
P. Bauer ◽  
E. Arzt ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Organic Molecules ◽  
Organic Matrix ◽  
Stable Form ◽  
Ambient Conditions ◽  
Calcium Carbonate Precipitation ◽  
Mineral Phases ◽  
Mineralization Processes ◽  
Co Precipitation

ABSTRACTVaterite is one of the thermodynamically less stable polymorphs of calcium carbonate. Under ambient conditions it transforms into calcite, the most stable form of calcium carbonate. Organisms are able to stabilize minerals such as vaterite by means of organic molecules. The exact mechanisms how biomineralization proteins interact with metastable mineral phases are, however, less well understood. Many in vitro studies were performed using calcite as a model system. A deeper understanding of the interaction of organic molecules with metastable mineral phases would make them useful as a tool to control mineralization processes in vitro. In this study, we report on the co-precipitation of a natively soluble histidine-tagged GFP (green fluorecent protein) with a metastable vaterite phase and the subsequent insolubility of the fluorescent organic matrix in a 30μl calcium carbonate precipitation assay. The intrinsic fluorescence of GFP is conserved during the interaction with the mineral phase, indicating proper folding even in the insoluble state. This experiment can be extended to obtain deeper insights into some mechanistic models of biomineralization proteins by tracking native and modified GFP proteins microscopically during various stages of mineral precipitation and dissolution.

Controlling calcium carbonate precipitation in the presence of biological and organic molecules

2001 ◽  
Author(s):  
Kathryn M. McGrath ◽  
Michael F. Barker ◽  
Steven R. Dickinson ◽  
G. Henderson ◽  
Callum R. MacKenzie ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Organic Molecules ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation

Attchment/Release of Phosphonate to/from a CaCO3 Surface in Supersaturated Brines

2014 ◽  
Author(s):  
Nan Zhang ◽  
Amy T. Kan ◽  
Mason B. Tomson
Keyword(s):  
Calcium Carbonate ◽  
Produced Water ◽  
Saturation Index ◽  
Extended Period ◽  
Calcium Carbonate Precipitation ◽  
Flowback Water ◽  
High Supersaturation ◽  
Co Precipitation ◽  
Kinetics Of ◽  
Theoretical Limitation

Abstract One of the most intractable concerns when engineers try to reuse the produced water as frac fluid in the Bakken and some other shale plays is the scale formation caused by the incompatibility of produced water with additives in the frac fluids and with the formation. In order to obtain a more efficient scale treatment for a successful hydraulic fracing that handles the extraordinary amount of water with high supersaturation level, the better understanding of inhibitor retention and release in the production system is urgent. To explore the mechanism of attachment/release of phosphonate to/from a mineral surface, calcite supersaturated feed solutions with different diethylenetriamine penta (DTPMP) concentrations were introduced into the steel tubing that was internally pre-coated with a thin layer of CaCO3. It is unveiled that DTPMP attachment was dominated by the precipitation of calcium phosphonate solid once the solution is supersaturated with Ca3H4DTPMP (pKsp=53.5), and the total amount of DTPMP attached on the calcite surface added up with the increasing supersaturation of Ca3H4DTPMP. The co-precipitation of CaCO3 and Ca3H4DTPMP has facilitated the attachment of the inhibitor with the increase of supersaturation of CaCO3. The retained phosphonate was released from the surface with a steady and low level inhibitor concentration over extended period of time. Combining with the kinetics of calcium carbonate precipitation in the presence of inhibitor, a 1500 gram of calcium phosphonate precipitation can protect the scaling for about 100 days (100 bbl/day) when the saturation index of calcium carbonate (SIcalcite) is as high as 1.3. The results provide a better understanding of calcium-phosphonate-carbonate interaction, and show the phosphonate inhibitor can continuously accumulate on the carbonate and slowly dissolve. We anticipate this study can shed a light on how much inhibitor can be delivered to the unconventional reservoir as well as the theoretical limitation of inhibitor return in the flowback water.

Calcium Carbonate Mineralization: Involvement of Extracellular Polymeric Materials Isolated from Calcifying Bacteria

2012 ◽  
Vol 18 (4) ◽  
pp. 829-839 ◽  
Author(s):  
Claudia Ercole ◽  
Paola Bozzelli ◽  
Fabio Altieri ◽  
Paola Cacchio ◽  
Maddalena Del Gallo
Keyword(s):  
Calcium Carbonate ◽  
Calcium Ions ◽  
Protein Profile ◽  
Synthetic Medium ◽  
Polymeric Materials ◽  
Crystal Formation ◽  
Calcium Carbonate Precipitation ◽  
Direct Role ◽  
Spectrometry Analysis

AbstractThis study highlights the role of specific outer bacterial structures, such as the glycocalix, in calcium carbonate crystallizationin vitro. We describe the formation of calcite crystals by extracellular polymeric materials, such as exopolysaccharides (EPS) and capsular polysaccharides (CPS) isolated fromBacillus firmusandNocardia calcarea. Organic matrices were isolated from calcifying bacteria grown on synthetic medium—in the presence or absence of calcium ions—and their effect on calcite precipitation was assessed. Scanning electron microscopy observations and energy dispersive X-ray spectrometry analysis showed that CPS and EPS fractions were involved in calcium carbonate precipitation, not only serving as nucleation sites but also through a direct role in crystal formation. The utilization of different synthetic media, with and without addition of calcium ions, influenced the biofilm production and protein profile of extracellular polymeric materials. Proteins of CPS fractions with a molecular mass between 25 and 70 kDa were overexpressed when calcium ions were present in the medium. This higher level of protein synthesis could be related to the active process of bioprecipitation.

scholarly journals Effect of Divalent Cations (Cu, Zn, Pb, Cd, and Sr) on Microbially Induced Calcium Carbonate Precipitation and Mineralogical Properties

2021 ◽  
Vol 12 ◽  
Author(s):  
Yumi Kim ◽  
Sunki Kwon ◽  
Yul Roh
Keyword(s):  
Calcium Carbonate ◽  
Removal Efficiency ◽  
Metal Removal ◽  
Divalent Cations ◽  
Growth Media ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Calcium Carbonates ◽  
Mineralogical Properties ◽  
Co Precipitation

Microbially induced calcium carbonate precipitation (MICP) is a bio-geochemical process involving calcium carbonate precipitation and possible co-precipitation of other metals. The study investigated the extent to which a urease-positive bacterium, Sporosarcina pasteurii, can tolerate a range of metals (e.g., Cu, Zn, Pb, Cd, and Sr), and analyzed the role of calcium carbonate bioprecipitation in eliminating these divalent toxicants from aqueous solutions. The experiments using S. pasteurii were performed aerobically in growth media including urea, CaCl2 (30 mM) and different metals such Cu, Zn, Pb, and Cd (0.01 ∼ 1 mM), and Sr (1 ∼ 30 mM). Microbial growth and urea degradation led to an increase in pH and OD600, facilitating the precipitation of calcium carbonate. The metal types and concentrations contributed to the mineralogy of various calcium carbonates precipitated and differences in metal removal rates. Pb and Sr showed more than 99% removal efficiency, whereas Cu, Zn, and Cd showed a low removal efficiency of 30∼60% at a low concentration of 0.05 mM or less. Thus the removal efficiency of metal ions during MICP varied with the types and concentrations of divalent cations. The MICP in the presence of divalent metals also affected the mineralogical properties such as carbonate mineralogy, shape, and crystallinity.

scholarly journals The Skeletal Organic Matrix from Mediterranean Coral Balanophyllia europaea Influences Calcium Carbonate Precipitation

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e22338 ◽  
Author(s):  
Stefano Goffredo ◽  
Patrizia Vergni ◽  
Michela Reggi ◽  
Erik Caroselli ◽  
Francesca Sparla ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Organic Matrix ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation

Lessons from Biomineralisation: the Role of Post-translational Modification

2021 ◽  
Author(s):  
◽  
Benjamin Matthewson
Keyword(s):  
Calcium Carbonate ◽  
Sea Urchin ◽  
Calcium Binding ◽  
Keratan Sulfate ◽  
Organic Matrix ◽  
Active Species ◽  
Protein Glycosylation ◽  
Post Translational Modification ◽  
Spin Filters

<p>In this thesis we present our findings following analysis of the acidic organic matrix (SMP) occluded in the calcite spines of the New Zealand sea urchin Evechinus chloroticus. The main focus involves correlation of the structure and function of the post-translational modifications (PTMs). The experimental framework developed to achieve this involved mapping the structure of the PTMs throughout SMP based on molecular weight (MW) followed by selective removal of each of the identified PTMs. The functional analysis involved the use of SMP, and its derivatives, as additives in an in vitro calcium carbonate crystallisation assay. The adoption of in vitro methods was considered appropriate as the focus of this work was to develop strategies towards programmable crystal growth in vitro. From analysis of the PTMs we have shown that there is extensive protein glycosylation, sulfation, and phosphorylation; all are involved in rendering the isoelectric point (pI) of the SMP macromolecules. The sulfates are exclusively housed on the glycan framework whereas the phosphate is protein bound. The majority of the SMP glycone is charged with O-glycosylation accounting for 80.0 +/- 4.0 wt%. The structure of the glycans includes sulfated HexNAc oligomers, and potentially mucin-like/keratan sulfate and/or carrageenan structures. Using Stains-All we have shown that the desulfated HexNAc oligomers have the ability to bind calcium which signals relevance in the formation of calcium carbonate. SMP was fractionated by MW across a series of spin-filters. Use of the various fractions in the crystallisation assay showed that the species in the greater than 30 kDa fraction held the ability to increase the number of crystals nucleated. In contrast, the macromolecules in the 10 to 30 kDa range contained the full complement of morphologically active species. The result that these functions can be isolated demonstrates that they are independently controlled. The structure-function relationships determined include: the protein and the acidic glycans are jointly sufficient to generate the nucleating function; deglycosylated SMP holds the complete morphological activity, however, the glycans contribute by increasing reproducibility presumably through regulatory influences; and the sterically hindered phosphate residues make a slight contribution to this morphological activity. These results indicate that analyses which involve characterisation of the morphological function of cloned biomineral proteins may indeed correspond to their native counterparts. The observation that the morphologically active species are phosphorylated identifies them as the calcium-binding phosphoproteins. The morphological activity of SMP stripped of all PTMs is equivalent to the proteins extracted from the aragonitic layer of Haliotis iris. Characterisation of SMP demonstrated similarities with the OMs of other sea urchin species. For example, SMP appears to include SM30. In addition, the overall structure of SMP includes abundant acidic glycosylation with a relatively neutral protein component. This structural make-up is in contrast to the highly acidic proteins which are barely post-translationally modified.</p>

Influences of Coral Intra-skeletal Organic Matrix on Calcium Carbonate Precipitation

2016 ◽  
pp. 207-222
Author(s):  
Michela Reggi ◽  
Simona Fermani ◽  
Oren Levy ◽  
Zvy Dubinsky ◽  
Stefano Goffredo ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Organic Matrix ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation
Sign in / Sign up

Export Citation Format

Share Document