Scanning Electron Microscopy and Surface Enhanced Raman Spectroscopy Correlation Studies of Functionalized Composite Organic-Inorganic SERS Nanoparticles on Cancer Cells

2011 ◽  
Vol 1316 ◽  
Author(s):  
Ai Leen Koh ◽  
Robert Sinclair
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Raman Spectroscopy ◽  
Cancer Cells ◽  
Surface Enhanced Raman Spectroscopy ◽  
Excitation Light ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Intensity ◽  
Scanning Electron

ABSTRACTComposite Organic-Inorganic Nanoparticles (COINs) are a novel type of surface-enhanced Raman (SER) scattering nanoparticle formed by aggregating inorganic silver particles in the presence of a chosen organic molecule with a distinct Raman fingerprint. Binding between antibody-functionalized COINs and cells is detected primarily using Raman spectroscopy, which measures spectral shifts of the excitation light due to inelastic scattering. It has been suggested that the amount of antibody-conjugated COINs binding on cells will vary according to the antigen-expression levels in cells and will lead to changes in measured SERS intensities. COINs functionalized with antibodies CD54 and CD8 were conjugated to U937 and SupT1 cancer cells and investigated in this study. SERS intensity measurements were obtained from each of the four sample variants and normalized against control samples comprising non-antibody-functionalized COINs with cells. The amount of COINs binding on cells was determined using scanning electron microscopy (SEM) and correlated with the SER spectroscopy intensity. Although we found a positive correlation between the number of COINs binding to cells and their respective SERS intensity, this relationship is not one-to-one, nor does it appear to be linear. We demonstrated that SEM imaging and SER spectroscopy can complement each other to provide information about COINs binding onto cancer cells.

Surface-enhanced Raman scattering (SERS) by Ag nanoparticles on anodized TiO2−X nanotubes

2019 ◽  
Vol 34 (01n03) ◽  
pp. 2040009
Author(s):  
Ting Zhang ◽  
Junzhi Ye ◽  
Rakesh Arul ◽  
Tingxuan Yang ◽  
Yixuan Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Raman Spectroscopy ◽  
Magnetron Sputtering ◽  
Ag Nanoparticles ◽  
Raman Enhancement ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Scanning Electron

In this work, Ag nanoparticles were deposited in the TiO[Formula: see text] nanotube films through magnetron sputtering to form Ag-TiO[Formula: see text] nanocomposite. Scanning electron microscopy (SEM) was used to study microstructure and surface morphology. Rhodamine B (RhB) was deposited on the surface of Ag-TiO[Formula: see text] substrate for Raman spectroscopy test. The size of Ag nanoparticles can be controlled by changing the magnetron sputtering time. The result indicated that sputtering for 30 s obtained the highest Raman enhancement.

scholarly journals Fabrication-friendly polarization-sensitive plasmonic grating for optimal surface-enhanced Raman spectroscopy

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Arpan Dutta ◽  
Tarmo Nuutinen ◽  
Khairul Alam ◽  
Antti Matikainen ◽  
Peng Li ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fill Factor ◽  
Surface Enhanced Raman Spectroscopy ◽  
Transverse Magnetic ◽  
Raman Signal ◽  
Optical Resonance ◽  
Excitation Light ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Plasmonic Gratings

Abstract Plasmonic nanostructures are widely utilized in surface-enhanced Raman spectroscopy (SERS) from ultraviolet to near-infrared applications. Periodic nanoplasmonic systems such as plasmonic gratings are of great interest as SERS-active substrates due to their strong polarization dependence and ease of fabrication. In this work, we modelled a silver grating that manifests a subradiant plasmonic resonance as a dip in its reflectivity with significant near-field enhancement only for transverse-magnetic (TM) polarization of light. We investigated the role of its fill factor, commonly defined as a ratio between the width of the grating groove and the grating period, on the SERS enhancement. We designed multiple gratings having different fill factors using finite-difference time-domain (FDTD) simulations to incorporate different degrees of spectral detunings in their reflection dips from our Raman excitation (488 nm). Our numerical studies suggested that by tuning the spectral position of the optical resonance of the grating, via modifying their fill factor, we could optimize the achievable SERS enhancement. Moreover, by changing the polarization of the excitation light from transverse-magnetic to transverse-electric, we can disable the optical resonance of the gratings resulting in negligible SERS performance. To verify this, we fabricated and optically characterized the modelled gratings and ensured the presence of the desired detunings in their optical responses. Our Raman analysis on riboflavin confirmed that the higher overlap between the grating resonance and the intended Raman excitation yields stronger Raman enhancement only for TM polarized light. Our findings provide insight on the development of fabrication-friendly plasmonic gratings for optimal intensification of the Raman signal with an extra degree of control through the polarization of the excitation light. This feature enables studying Raman signal of exactly the same molecules with and without electromagnetic SERS enhancements, just by changing the polarization of the excitation, and thereby permits detailed studies on the selection rules and the chemical enhancements possibly involved in SERS.

Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip

Lab on a Chip ◽  
2017 ◽  
Vol 17 (7) ◽  
pp. 1306-1313 ◽  
Author(s):  
Zhimin Zhai ◽  
Fengqiu Zhang ◽  
Xiangyu Chen ◽  
Jie Zhong ◽  
Gang Liu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Microfluidic Chip ◽  
Surface Enhanced Raman Spectroscopy ◽  
Living Cells ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

This paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS).

scholarly journals Rapid Detection of Tetracycline Residues in Duck Meat Using Surface Enhanced Raman Spectroscopy

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Jinhui Zhao ◽  
Ping Liu ◽  
Haichao Yuan ◽  
Yijie Peng ◽  
Qian Hong ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Rapid Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Detection Scheme ◽  
Meat Extract ◽  
Relative Standard ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Tetracycline Residues ◽  
Sers Intensity

A rapid detection method based on surface enhanced Raman spectroscopy (SERS) was proposed in this paper in order to realize the detection of tetracycline residues in duck meat. Firstly, surface enhanced Raman spectra characteristics of tetracycline aqueous solution, duck meat extract, and duck meat extract containing tetracycline were analyzed. Secondly, the effect of the addition amount of duck meat extract containing tetracycline on SERS intensity and the effect of the adsorption time on SERS intensity were discussed, respectively. Thirdly, SERS intensity ratio at 1272 and 1558 cm−1 (I1272/I1558) was used to establish the SERS calibration curve. A good linearity relationship between the tetracycline concentration in duck meat extract and I1272/I1558 was obtained, and the linear regression equation and the correlation coefficient (r) were y=0.0177x+0.1213 and 0.950, respectively. The average recovery of tetracycline in duck meat extract was 101~108% with relative standard deviation (RSD) of 2.4~4.6%. The experimental results showed that the method proposed in this paper was a good detection scheme for the rapid detection of tetracycline residues in duck meat.

scholarly journals Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced Raman spectroscopy

2016 ◽  
Vol 1 (1) ◽  
pp. 60-63 ◽  
Author(s):  
Jonathan Simpson ◽  
Derek Craig ◽  
Karen Faulds ◽  
Duncan Graham
Keyword(s):  
Raman Spectroscopy ◽  
Cholera Toxin ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Intensity

Cholera toxin B subunit (CTB) is detected sensitively in samples of synthetic freshwater by measuring changes in the SERS intensity of the glyconanoparticles upon their binding of CTB and subsequently aggregating. The particles are coated in both galactose and sialic acid.

Sign in / Sign up

Export Citation Format

Share Document