Characterization of Erythrocytes in the Sickle Cell Trait

2011 ◽  
Vol 1301 ◽  
Author(s):  
Jamie L. Maciaszek ◽  
George Lykotrafitis
Keyword(s):  
Sickle Cell ◽  
Single Molecule ◽  
Protein Interactions ◽  
Sickle Cell Trait ◽  
Human Subjects ◽  
Protein Protein Interactions ◽  
Force Microscopy ◽  
Laminin Receptors ◽  
Atomic Force ◽  
Normal Human

ABSTRACTAtomic force microscopy (AFM) allows for high-resolution topography studies of biological cells, measurement of their mechanical properties, and quantification of protein-protein interactions in physiological conditions. In this work, AFM was employed to investigate morphological, material, and chemomechanical properties of red blood cells from human subjects with sickle cell trait. We measured the stiffness of the cells and demonstrated that the Young’s modulus of pathological erythrocytes was three times greater than in normal cells. A single molecule AFM method was employed to report that erythrocytes from human subjects with the sickle cell trait express a greater number of the laminin receptors BCAM/Lu (p < 0.05) than erythrocytes from normal human subjects. Observed differences indicate the effect of sickle hemoglobin in the erythrocyte and possible changes in the organization of the cell cytoskeleton and membrane proteins associated with the sickle cell trait.

Nanoscale Analysis of the Effect of Pathogenic Mutations on Polycystin-1

2010 ◽  
Author(s):  
Liang Ma ◽  
Meixiang Xu ◽  
Andres F. Oberhauser
Keyword(s):  
Single Molecule ◽  
Conformational Changes ◽  
Protein Interactions ◽  
Structure And Function ◽  
Eukaryotic Cells ◽  
Mechanical Forces ◽  
Force Microscopy ◽  
Physiological Conditions ◽  
Atomic Force ◽  
And Function

The activity of proteins and their complexes often involves the conversion of chemical energy (stored or supplied) into mechanical work through conformational changes. Mechanical forces are also crucial for the regulation of the structure and function of cells and tissues. Thus, the shape of eukaryotic cells is the result of cycles of mechano-sensing, mechano-transduction, and mechano-response. Recently developed single-molecule atomic force microscopy (AFM) techniques can be used to manipulate single molecules, both in real time and under physiological conditions, and are ideally suited to directly quantify the forces involved in both intra- and intermolecular protein interactions. In combination with molecular biology and computer simulations, these techniques have been applied to characterize the unfolding and refolding reactions in a variety of proteins, such as titin (an elastic mechano-sensing protein found in muscle) and polycystin-1 (PC1, a mechanosensor found in the kidney).

scholarly journals High-Speed Atomic Force Microscopy of Protein-Protein Interactions

2013 ◽  
Vol 104 (2) ◽  
pp. 386a
Author(s):  
Simon Scheuring ◽  
Ignacio Casuso ◽  
Felix Rico ◽  
Adai Colom
Keyword(s):  
Atomic Force Microscopy ◽  
Protein Interactions ◽  
High Speed ◽  
Protein Protein Interactions ◽  
Force Microscopy ◽  
Atomic Force

Modulation of BCAM/Lu and ICAM-4 Expression on Red Blood Cell Membranes in Physiological Stress Conditions

2011 ◽  
Author(s):  
Jamie L. Maciaszek ◽  
Biree Andemariam ◽  
George Lykotrafitis
Keyword(s):  
Single Molecule ◽  
Sickle Cell Trait ◽  
Physiological Stress ◽  
Physical Exertion ◽  
Strenuous Exercise ◽  
Intercellular Adhesion Molecule ◽  
Force Microscopy ◽  
Atomic Force ◽  
Red Blood Cell Membranes

Using single-molecule atomic force microscopy (AFM) experiments, we report that epinephrine, a hormone secreted during stress and strenuous exercise, increases both the frequency and strength of adhesion events of sickle cell trait erythrocytes to the endothelial extracellular matrix (ECM). The specific interactions quantified include Lutheran (BCAM/Lu) and intercellular adhesion molecule-4 (ICAM-4) on the RBC surface with ECM laminin and endothelial αvβ3, respectively. These data present significant evidence of the role of epinephrine in BCAM/Lu-laminin and ICAM-4-αvβ3 bonding, and suggest mechanisms of vaso-occlusion during physical exertion in SCT.

Sickle Cell Trait Erythrocytes are Thrice as Stiff as Healthy Erythrocytes

2010 ◽  
Author(s):  
Jamie L. Maciaszek ◽  
George Lykotrafitis
Keyword(s):  
Young’S Modulus ◽  
Sickle Cell ◽  
Blood Cells ◽  
Healthy Subjects ◽  
Sickle Cell Trait ◽  
Young's Modulus ◽  
Force Microscopy ◽  
Physiological Conditions ◽  
Human Red Blood Cells ◽  
Atomic Force

Atomic force microscopy (AFM) allows for high-resolution topography studies of biological cells and measurement of their mechanical properties in physiological conditions. In this work, AFM was employed to measure the stiffness of abnormal human red blood cells (RBCs) from patients with the genotype for sickle cell trait. The determined Young’s modulus was compared with that obtained from measurements of erythrocytes from healthy subjects. The results showed that the Young’s modulus of pathological erythrocytes was approximately three times higher than in normal cells. Observed differences indicate the effect of hemoglobin S as well as possible changes in the organization of the cell cytoskeleton associated with the sickle cell trait.

Atomic Force Microscopy (AFM) for Topography and Recognition Imaging at Single Molecule Level

2013 ◽  
pp. 102-112
Author(s):  
Memed Duman ◽  
Andreas Ebner ◽  
Christian Rankl ◽  
Jilin Tang ◽  
Lilia A. Chtcheglova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Recognition Imaging
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