Processing of biphasic calcium phosphate ceramics for culturing of bone marrow stem cells

2017 ◽  
Vol 32 (17) ◽  
pp. 3260-3270 ◽  
Author(s):  
Qinghao Zhang ◽  
Qi Jiapeng ◽  
Wenfu Wang ◽  
Ian Nettleship
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Bone Marrow Stem Cells ◽  
Calcium Phosphate Ceramics ◽  
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Abstract

scholarly journals Chondrogenic and BMP-4 primings confer osteogenesis potential to human cord blood mesenchymal stromal cells delivered with biphasic calcium phosphate ceramics

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Meadhbh Á. Brennan ◽  
Mario Barilani ◽  
Francesco Rusconi ◽  
Julien de Lima ◽  
Luciano Vidal ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Cord Blood ◽  
Stromal Cells ◽  
Biphasic Calcium Phosphate ◽  
Bone Repair ◽  
Calcium Phosphate Ceramics

AbstractBone marrow mesenchymal stem/stromal cells (BMSCs) show great promise for bone repair, however they are isolated by an invasive bone marrow harvest and their regenerative potential decreases with age. Conversely, cord blood can be collected non-invasively after birth and contains MSCs (CBMSCs) that can be stored for future use. However, whether CBMSCs can replace BMSCs targeting bone repair is unknown. This study evaluates the in vitro osteogenic potential of unprimed, osteogenically primed, or chondrogenically primed CBMSCs and BMSCs and their in vivo bone forming capacity following ectopic implantation on biphasic calcium phosphate ceramics in nude mice. In vitro, alkaline phosphatase (intracellular, extracellular, and gene expression), and secretion of osteogenic cytokines (osteoprotegerin and osteocalcin) was significantly higher in BMSCs compared with CBMSCs, while CBMSCs demonstrated superior chondrogenic differentiation and secretion of interleukins IL-6 and IL-8. BMSCs yielded significantly more cell engraftment and ectopic bone formation compared to CBMSCs. However, priming of CBMSCs with either chondrogenic or BMP-4 supplements led to bone formation by CBMSCs. This study is the first direct quantification of the bone forming abilities of BMSCs and CBMSCs in vivo and, while revealing the innate superiority of BMSCs for bone repair, it provides avenues to induce osteogenesis by CBMSCs.

Bone marrow stem cells make hepatocytes with replicative potential

2001 ◽  
Vol 120 (5) ◽  
pp. A62-A62
Author(s):  
S FORBES ◽  
M ALISON ◽  
K HODIVALADILKE ◽  
R JEFFERY ◽  
R POULSOM ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells

Autologous 5-azacytidine-cultured bone-marrow stem cells in surgical treatment of heart failure

2008 ◽  
Vol 7 ◽  
pp. 114-115
Author(s):  
R AKCHURIN ◽  
T RAKHMATZADE ◽  
E SKRIDLEVSKAYA ◽  
L SAMOYLENKO ◽  
V SERGIENKO ◽  
...  
Keyword(s):  
Heart Failure ◽  
Stem Cells ◽  
Bone Marrow ◽  
Surgical Treatment ◽  
Bone Marrow Stem Cells
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