Tunable glass reference materials for quantitative backscattered electron imaging of mineralized tissues

2012 ◽  
Vol 27 (19) ◽  
pp. 2568-2577 ◽  
Author(s):  
Sara E. Campbell ◽  
Roy H. Geiss ◽  
Steve A. Feller ◽  
Virginia L. Ferguson
Keyword(s):  
Reference Materials ◽  
Backscattered Electron Imaging ◽  
Quantitative Backscattered Electron Imaging ◽  
Mineralized Tissues ◽  
Backscattered Electron ◽  
Image Position ◽  
Electron Imaging

Abstract

scholarly journals Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 732
Author(s):  
Karol Alí Apaza Alccayhuaman ◽  
Stefan Tangl ◽  
Stéphane Blouin ◽  
Markus A. Hartmann ◽  
Patrick Heimel ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Formation ◽  
Collagen Matrix ◽  
Microcomputed Tomography ◽  
Soft Tissue Augmentation ◽  
Backscattered Electron Imaging ◽  
Quantitative Backscattered Electron Imaging ◽  
Rat Calvaria ◽  
Backscattered Electron ◽  
Electron Imaging

Volume-stable collagen matrices (VSCM) are conductive for the connective tissue upon soft tissue augmentation. Considering that collagen has osteoconductive properties, we have investigated the possibility that the VSCM also consolidates with the newly formed bone. To this end, we covered nine rat calvaria circular defects with a VSCM. After four weeks, histology, histomorphometry, quantitative backscattered electron imaging, and microcomputed tomography were performed. We report that the overall pattern of mineralization inside the VSCM was heterogeneous. Histology revealed, apart from the characteristic woven bone formation, areas of round-shaped hypertrophic chondrocyte-like cells surrounded by a mineralized extracellular matrix. Quantitative backscattered electron imaging confirmed the heterogenous mineralization occurring within the VSCM. Histomorphometry found new bone to be 0.7 mm2 (0.01 min; 2.4 max), similar to the chondrogenic mineralized extracellular matrix with 0.7 mm2 (0.0 min; 4.2 max). Microcomputed tomography showed the overall mineralized tissue in the defect to be 1.6 mm3 (min 0.0; max 13.3). These findings suggest that in a rat cranial defect, VSCM has a limited and heterogeneous capacity to support intramembranous bone formation but may allow the formation of bone via the endochondral route.

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