Titanium nanoparticles phytosynthesized from Eichhornia crassipes leaf extract and their antimicrobial activity.

MRS Advances ◽  
2020 ◽  
Vol 5 (62) ◽  
pp. 3293-3299
Author(s):  
Monserrat Velázquez-Hernández ◽  
Pablo Schabes-Retchkiman ◽  
Sonia Martínez-Gallegos ◽  
V. Albiter
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Eichhornia Crassipes ◽  
Leaf Extract ◽  
Plate Count ◽  
Tem Analysis ◽  
E Coli ◽  
Solid Agar ◽  
Bacteriological Test ◽  
Titanium Nanoparticles

AbstractIn this study, we reported an environmentally friendly technique for the synthesis of titanium nanoparticles using Eichhornia crassipes leaf extract as a non-toxic reducing agent and efficient stabilizer. Also the antimicrobial activity TiNPs against E. coli. Bacteriological test were performed on solid agar plates with different concentrations of TiNPs. On the other hand TiNPs were characterized by UV-visible spectroscopy, EDS (Dispersive Energy X-ray Spectroscopy), SEM (Scanning Electron Microscopy), and TEM (Transmission Electron Microscopy). It was found that TiNPs exhibit high crystallinity whit rutile titania structure. TEM analysis shows the nanoparticle size in the range from 22 to 44 nm, antimicrobial study was performed by plate count technique witch showed >99% mortality for E. coli bacteria studied after 24 h of incubation.

scholarly journals An assessment of the wound healing potential of a herbal gel containing an Azadirachta indica leaf extract

2021 ◽  
Vol 66 (No. 3) ◽  
pp. 99-109
Author(s):  
M Munir ◽  
SNH Shah ◽  
U Almas ◽  
FA Khan ◽  
A Zaidi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Leaf Extract ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Physicochemical Characteristics ◽  
Wound Healing Process ◽  
E Coli ◽  
Carbopol 940 ◽  
Gel Formulations

The objective of this study was to produce a Carbopol 940 based gel formula containing an Azadirachta indica leaf extract and evaluate its wound healing potential. The ethanolic extract was derived from the dried leaves of Azadirachta indica and was subjected to a phytochemical evaluation. Three gel formulations of Carbopol 940 containing an Azadirachta indica extract in three different concentrations, i.e., 1, 2, and 3% w/w were prepared. These gels were evaluated for their physical appearance, stability, antimicrobial activity, extrudability, skin irritability, pH, spreadability, and viscosity. The prepared formulas were stable, greenish and homogeneous. None of them showed irritation to the skin. The spreadability (g.cm/sec), viscosity (cps), and pH of all three formulations was 34.68, 53 270–65 400, and 6–7, respectively. Gel-III exhibited the highest antimicrobial potential against E. coli and P. aeruginosa with a zone of inhibition of 16.2 ± 0.6 mm and 15.6 ± 0.6 mm, respectively. It was revealed from the wound healing studies that the epithelialisation time for the Albino rabbits treated with Gel-III was 23 days. The Albino rabbits treated with Gel-I, Gel-II, a standard gel, and those with the untreated one (control), epithelialised in 27, 25, 26, and 34 days, respectively. A formulation containing 3% w/w extract showed better antimicrobial activity, physicochemical characteristics, and pharmacological parameters than the other formulations. It can be concluded that the wound healing process was faster with the gel formulation containing 3% w/w of the Azadirachta indica extract, proposing that this formulation is a promising candidate for wound healing.

scholarly journals PSIDIUM GUAJAVA: A NOVEL PLANT IN THE SYNTHESIS OF SILVER NANOPARTICLES FOR BIOMEDICAL APPLICATIONS.

2018 ◽  
Vol 11 (1) ◽  
pp. 341
Author(s):  
Sharmila C ◽  
Ranjith Kumar R ◽  
Chandar Shekar B
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Biomedical Applications ◽  
Leaf Extract ◽  
Cost Effective ◽  
Psidium Guajava ◽  
Tem Analysis ◽  
X Ray ◽  
Green Route

 Objective: Synthesis of silver nanoparticles (AgNPs) using a simple, cost-effective and environmentally friendly green route approach and to study the antibacterial activity of AgNPs against human pathogens.Methods: Green route approach is used to synthesize AgNPs using Psidium guajava leaf extract. Fourier transform infrared (FTIR) was used to identify the presence of the functional group. X-ray diffraction (XRD) was used to analyze the structure of prepared AgNPs. Energy dispersive X-ray was used to the characteristic to the composition of the prepared nanoparticles. Size and morphology of the prepared AgNPs were investigated using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) analysis. Antibacterials efficiency of prepared AgNPs was tested against Escherichia coli and Staphylococcus aureus by well diffusion methods.Results: FTIR study shows the presence of different functional groups present in the leaves mediated AgNPs. The XRD studies yield diffraction peaks corresponding to face-centered cubic structure of Ag crystals. Spherical shaped AgNPs with a particle size of about ~55 nm were evidenced using FESEM and TEM analysis. Energy dispersive spectrum of the synthesized AgNPs confirms the presence of silver in the prepared nanoparticles. From UV-VIS analysis it is shown that the absorption band was red-shifted from 430 nm to 456 nm. The prepared AgNPs shows good antibacterial activity against E. coli and S. aureus.Conclusions: P. guajava leaf extract is a potential reducing agent to synthesize AgNPs. The green synthesis approach provides cost-effective and eco-friendly nanoparticles, which could be used in biomedical applications.

scholarly journals Bacterial Mediated Rapid and Facile Synthesis of Silver Nanoparticles and Their Antimicrobial Efficacy against Pathogenic Microorganisms

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2615
Author(s):  
Md. Amdadul Huq ◽  
Shahina Akter
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Field Emission ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Gram Positive ◽  
Tem Analysis ◽  
Gram Negative ◽  
X Ray

In the present study, silver nanoparticles (AgNPs), biosynthesized using culture supernatant of bacterial strain Paenarthrobacter nicotinovorans MAHUQ-43, were characterized and their antimicrobial activity was investigated against both Gram-positive Bacillus cereus and Gram-negative bacteria Pseudomonas aeruginosa. Bacterial-mediated synthesized AgNPs were characterized by UV-Visible (UV-Vis) spectrophotometer, field emission-transmission electron microscopy (FE-TEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) analysis. The UV-Vis spectral analysis showed the absorption maxima at 466 nm which assured the synthesis of AgNPs. The FE-TEM analysis revealed the spherical shape of nanoparticles with the size range from 13 to 27 nm. The EDX and XRD analysis ensured the crystalline nature of biosynthesized AgNPs. The FTIR analysis revealed the involvement of different biomolecules for the synthesis of AgNPs as reducing and capping agents. The bacterial-mediated synthesized AgNPs inhibited the growth of pathogenic strains B. cereus and P. aeruginosa and developed a clear zone of inhibition (ZOI). The MIC and MBC for both pathogens were 12.5 µg/mL and 25 µg/mL, respectively. Moreover, field emission scanning electron microscopy analysis revealed that the synthesized AgNPs can destroy the outer membrane and alter the cell morphology of treated pathogens, leading to the death of cells. This study concludes the eco-friendly, facile and rapid synthesis of AgNPs using P. nicotinovorans MAHUQ-43 and synthesized AgNPs showed excellent antimicrobial activity against both Gram-positive and Gram-negative pathogens.

scholarly journals Areca Catechu Leaf Extract-Mediated Synthesis of Stabilized Silver Doped-Copper Oxide Nanoparticles and its Biomedical Applications

2021 ◽  
Author(s):  
Shwetha UR ◽  
Virupaxappa S. Betageri ◽  
Latha M.S. ◽  
Ravindra Veerapur ◽  
Chandan Shivamallu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Crystalline Structure ◽  
Leaf Extract ◽  
Cell Model ◽  
Research Work ◽  
Antidiabetic Activity ◽  
Solution Combustion ◽  
Tem Analysis ◽  
Cuo Nps ◽  
Areca Catechu

Abstract The current research work gives an insight into the green synthesis of metallic nanoparticle using medicinally important plant, which has gained increasing applications in therapeutics and medicinal science. Herein, we describe an eco-friendly method to synthesize Ag- CuO nanoparticles using Areca catechu leaf extract via solution combustion method. Crystalline structure, size and surface morphology of the synthesized nanoparticles were confirmed by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), Transmission Electron Microscopy (TEM) and Fourier Transformer Infrared Spectroscopy (FTIR). XRD patterns of the as-prepared Ag-CuO NPs revealed that they possess monoclinic crystalline structure. The morphology and the elemental composition was found by SEM-EDS and TEM analysis, which revealed the nano-spherical shape of Ag-CuO NPs. Further, the Ag-CuO NPs were tested for Antidiabetic activity by using Yeast cell model and amalyze inhibition, which showed promising antidiabetic activity. In addition, the as-prepared material displayed significant anticancer activity against MCF-7 cancer cell line.

scholarly journals Increasing the Shelf Life of Milk by Metal Oxide Nanoparticles and Mild Heat

2020 ◽  
Author(s):  
Mahbooubeh Mirhosseini ◽  
Roghayeh Dehestani
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Metal Oxide ◽  
Pathogenic Bacteria ◽  
Morphological Changes ◽  
Synergistic Effects ◽  
Metal Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
E Coli ◽  
Mild Heat

Background: The spread of pathogenic microorganisms in food and beverage and their resistance to antibiotics have raised major concerns for public health. The aim of this study was to investigate the antimicrobial activity of various metal oxide nanoparticles (NPs) including zinc oxide (ZnO), magnesium oxide (MgO), and iron oxide (Fe2O3) NPs against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Furthermore, the antimicrobial activity of these NPs in milk was studied along with mild heat. Methods: In this experimental study, the antibacterial activity of ZnO, MgO, and Fe2O3 NPs were initially evaluated by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. Later, the antimicrobial effect of these NPs was investigated in milk along with mild heating. To determine the morphological changes in S. aureus and E. coli, electron microscopy scanning was applied before and after the antimicrobial treatments. Results: The MBC and MIC values presented by Fe2O3, ZnO, and MgO NPs against pathogenic bacteria showed that MgO NPs were the most potent substances for inhibiting the growth of S. aureus and E. coli. The results also indicated that use of these NPs had synergistic effects in combination with the heating treatment. Electron microscopy scanning also revealed that treatment with MgO NPs could distort and impair the cell wall of the pathogenic bacteria, leading to the leakage of intracellular components and bacterial death. Conclusion: The results suggest that MgO, ZnO, and Fe2O3 NPs can be applied for industrial food processing as effective antimicrobial compounds to decrease the temperature required for pasteurizing milk.

SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF PERGULARIA DAEMIA AND ANALYSIS OF ANTIMICROBIAL ACTIVITY

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (08) ◽  
pp. 25-29
Author(s):  
Jambuwant A. Kadam ◽  
Mahesh A Karale ◽  
Pushpa Karale
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Visual Observation ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
E Coli ◽  
Antimicrobial Potential ◽  
Transmission Electron ◽  
Research Findings

The present work deals with the green synthesis of silver nanoparticle from aqueous extract of Pergularia daemia as reducing agent and evaluation of the antimicrobial potential of synthesized green nanoparticles (GNPs). The synthesized silver nanoparticles (SNPs) were characterized by UltravioletVisible absorption spectroscopy (UV-Vis) and high-resonance transmission electron microscopy (TEM) analysis. Visual observation showed that the color of the fresh leaf extracts of P. daemia turned into dark brown after incubation of 24 h with Ag precursors. The TEM analysis showed that nanoparticles were spherical in shape and the size was found to be in the range of 7-22 nm. The green synthesized nanoparticles showed concentration dependent (25 µg/mL, 50 µg/mL and 100 µg/mL) noteworthy antimicrobial activity against E. coli, S. aureus and B. subtilis with ciprofloxacin as a standard. Research findings conclude that GNPs possess superior antimicrobial potential and it is a new option to combat antibiotic resistance.

scholarly journals Marine Alkaloid 2,2-Bis(6-bromo-3-indolyl) Ethylamine and Its Synthetic Derivatives Inhibit Microbial Biofilms Formation and Disaggregate Developed Biofilms

2019 ◽  
Vol 7 (2) ◽  
pp. 28 ◽  
Author(s):  
Raffaella Campana ◽  
Gianfranco Favi ◽  
Wally Baffone ◽  
Simone Lucarini
Keyword(s):  
Antimicrobial Activity ◽  
Plate Count ◽  
Side Chain ◽  
Bacterial Populations ◽  
E Coli ◽  
Microbial Biofilms ◽  
Marine Alkaloid ◽  
Natural Alkaloid ◽  
Bisindole Alkaloid ◽  
Synthetic Derivatives

The antimicrobial activity of the marine bisindole alkaloid 2,2-bis(6-bromo-3-indolyl) ethylamine (1) and related synthetic analogues (compounds 2–8) against target microorganisms was investigated by Minimum Inhibitory Concentration (MIC) determination. Compound 1 showed the greatest antimicrobial activity with the lowest MIC (8 mg/L) against Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae, while the derivatives exhibited higher MICs values (from 16 to 128 mg/L). Compounds 1, 3, 4, and 8, the most active ones, were then tested against E. coli, S. aureus, K. pneumoniae, and Candida albicans during biofilms formation as well as on 24 h developed biofilms. The natural alkaloid 1 inhibited the biofilm formation of all the tested microorganisms up to 82.2% and disaggregated biofilms of E. coli, S. aureus, K. pneumoniae, and C. albicans after 30 min of contact, as assessed by viable plate count and crystal violet (CV) staining (optical density at 570 nm). Synthetic derivatives 3, 4, and 8 displayed anti-biofilm activity toward individual bacterial populations. This study highlights the potential of marine bisindole alkaloid 1 as anti-biofilm agent and shows, through a preliminary structure activity relationship (SAR), the importance of halogens and ethylamine side chain for the antimicrobial and antibiofilm activities of this bisindole series.

scholarly journals Controlling the Antimicrobial Action of Surface Modified Magnesium Hydroxide Nanoparticles

Biomimetics ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 41 ◽  
Author(s):  
Ahmed F. Halbus ◽  
Tommy S. Horozov ◽  
Vesselin N. Paunov
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Magnesium Hydroxide ◽  
Inorganic Nanoparticles ◽  
X Ray Diffraction ◽  
X Ray ◽  
E Coli ◽  
Transmission Electron ◽  
Surface Modified ◽  
Magnesium Hydroxide Nanoparticles

Magnesium hydroxide nanoparticles (Mg(OH)2NPs) have recently attracted significant attention due to their wide applications as environmentally friendly antimicrobial nanomaterials, with potentially low toxicity and low fabrication cost. Here, we describe the synthesis and characterisation of a range of surface modified Mg(OH)2NPs, including particle size distribution, crystallite size, zeta potential, isoelectric point, X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), energy dispersive X-ray analysis (EDX), Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). We explored the antimicrobial activity of the modified Mg(OH)2NPs on the microalgae (C. reinhardtii), yeast (S. cerevisiae) and Escherichia coli (E. coli). The viability of these cells was evaluated for various concentrations and exposure times with Mg(OH)2NPs. It was discovered that the antimicrobial activity of the uncoated Mg(OH)2NPs on the viability of C. reinhardtii occurred at considerably lower particle concentrations than for S. cerevisiae and E. coli. Our results indicate that the antimicrobial activity of polyelectrolyte-coated Mg(OH)2NPs alternates with their surface charge. The anionic nanoparticles (Mg(OH)2NPs/PSS) have much lower antibacterial activity than the cationic ones (Mg(OH)2NPs/PSS/PAH and uncoated Mg(OH)2NPs). These findings could be explained by the lower adhesion of the Mg(OH)2NPs/PSS to the cell wall, because of electrostatic repulsion and the enhanced particle-cell adhesion due to electrostatic attraction in the case of cationic Mg(OH)2NPs. The results can be potentially applied to control the cytotoxicity and the antimicrobial activity of other inorganic nanoparticles.

scholarly journals De-novo fabrication of sunlight irradiated silver nanoparticles and their efficacy against E. coli and S. epidermidis

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Hammad Arshad ◽  
Saima Sadaf ◽  
Umer Hassan
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
De Novo ◽  
Cost Effective ◽  
Microbial Pathogens ◽  
Solar Irradiation ◽  
Root Extract ◽  
Salvadora Persica ◽  
Tem Analysis ◽  
E Coli

AbstractSilver nanoparticles (AgNPs) gained significant attention due to their activity against microbial pathogens, cancer cells, and viral particles etc. Traditional fabrication methods require hazardous chemicals as reducing agents and their usage and disposal pose a significant hazard to environmental ecosystem. Here, a de novo, robust, cost effective and an eco-friendly method is reported to fabricate AgNPs irradiated with sunlight (SL) while using Salvadora persica root extract (SPE) as reducing agent. Sunlight (SL) irradiated S. persica silver nanoparticles (SpNPs) i.e., SL-SpNPs were characterized using multiple techniques and their antibacterial efficacy was evaluated. The SL-SpNPs were synthesized in 10 min. Field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) analysis revealed their spherical morphology with a size range of 4.5–39.7 nm, while surface plasmon resonance (SPR) peaked at 425 nm. Fourier transform infrared spectroscopy (FTIR) analysis suggested that the reduction of SL-SpNPs was due to the presence of phytochemicals in the SPE. Furthermore, X-ray powder diffraction (P-XRD) pattern depicted the crystal structure of SL-SpNPs, hence proving the presence of AgNPs. Further the antibacterial studies were carried out against Escherichia coli (ATCC 11229) and Staphylococcus epidermidis (ATCC 12228) using Kirby Bauer method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for E. coli were determined to be 1.5 μg/mL and 3.0 μg/mL respectively while MIC and MBC values for S. epidermidis were found to be 12.5 μg/mL and 25 μg/mL respectively. The solar irradiation-based fabrication method and resulting SL-SpNPs can find their utility in many biomedical and environmental applications.

scholarly journals Evaluation of Antimicrobial activity and Genotoxic Potential of Capparis spinosa (L.) Plant Extracts

2021 ◽  
pp. 48-57
Author(s):  
Ghaleb M. Adwan ◽  
Ghadeer Ibrahim Omar
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Fungal Infections ◽  
Bacterial Species ◽  
Genotoxic Effect ◽  
Enterobacterial Repetitive Intergenic Consensus ◽  
E Coli ◽  
Rapd Pcr ◽  
Capparis Spinosa ◽  
Aqueous Leaf Extract

Objective: The aims of this study were to evaluate the antimicrobial activity and the genotoxic effect of both ethanolic and aqueous extracts of stem and leaf of Capparis spinosa (C. spinosa) plant on Escherichia coli (E. coli) ATCC 25922, Staphylococcus aureus (S. aureus) ATCC 6538P, clinical isolate of Methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae (K. pneumoniae) and Candida albicans (C. albicans) ATCC 90028. Materials and Methods: The antimicrobial activity was determined using microbroth dilution method, while the genotoxic effect was investigated using randomly amplified polymorphic DNA (RAPD)-PCR and enterobacterial repetitive intergenic consensus (ERIC)-PCR. Results: The MIC values of both ethanolic and aqueous leaf and stem extracts of C. spinosa plant had a range 6.25 mg/ml to 100 mg/ml. In addition, it was found that ethanolic extract more effective than aqueous extract. The genotoxic activity of aqueous leaf extract, showed changes in both Random Amplified Polymorphic DNA (RAPD)-PCR and Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR profiles of E. coli strain treated with extract compared to untreated (negative) control. These changes included an alteration in the intensity, absence or appearance of new amplified fragments. Conclusions: Results of this study strongly show the genotoxic effect of aqueous leaf extract from C. spinosa plant on E. coli. The findings draw awareness to the possible toxic effect use of C. spinosa plant in traditional medicine and point out the capability of using C. spinosa to treat bacterial or fungal infections. More studies are needed to detect the exact ingredients of this plant as well as the mechanisms responsible for genotoxicity. Further in vivo genotoxicity studies are recommended to ensure and to evaluate the safety of using plants for therapeutic purposes. In addition, results of this study showed that molecular fingerprinting based on ERIC-PCR can be used to evaluate the genotoxic effect in the model bacterial species E. coli.

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