scholarly journals Identification of Porphyrin-Silica Composite Nanoparticles using Atmospheric Solids Analysis Probe Mass Spectrometry

MRS Advances ◽  
2019 ◽  
Vol 4 (38-39) ◽  
pp. 2079-2086
Author(s):  
Casey Karler ◽  
Kylea J. Parchert ◽  
James B. Ricken ◽  
Bryan Carson ◽  
Curtis D. Mowry ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cellular Uptake ◽  
Hela Cells ◽  
Energy Transduction ◽  
Composite Nanoparticles ◽  
Growth Media ◽  
Glass Capillary ◽  
Silica Composite ◽  
Novel Method ◽  
Solids Analysis

ABSTRACTPorphyrins are vital pigments involved in biological energy transduction processes. Their abilities to absorb light, then convert it to energy, have raised the interest of using porphyrin nanoparticles as photosensitizers in photodynamic therapy. A recent study showed that self- assembled porphyrin-silica composite nanoparticles can selectively destroy tumor cells, but detection of the cellular uptake of porphyrin-silica composite nanoparticles was limited to imaging microscopy. Here we developed a novel method to rapidly identify porphyrin-silica composite nanoparticles using Atmospheric Solids Analysis Probe-Mass Spectrometry (ASAP-MS). ASAP-MS can directly analyze complex mixtures without the need for sample preparation. Porphyrin-silica composite nanoparticles were vaporized using heated nitrogen desolvation gas, and their thermo-profiles were examined to identify distinct mass- to-charge (M/Z) signatures. HeLa cells were incubated in growth media containing the nanoparticles, and after sufficient washing to remove residual nanoparticles, the cell suspension was loaded onto the end of ASAP glass capillary probe. Upon heating, HeLa cells were degraded and porphyrin-silica composite nanoparticles were released. Vaporized nanoparticles were ionized and detected by MS. The cellular uptake of porphyrin-silica composite nanoparticles was identified using this ASAP-MS method.

scholarly journals First Evidence of “Earth Wax” Inside the Casting Molds from the Roman Era

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4259
Author(s):  
Klára Jagošová ◽  
Jan Jílek ◽  
Pavel Fojtík ◽  
Ivan Čižmář ◽  
Miroslav Popelka ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution Mass Spectrometry ◽  
Intermediate Stage ◽  
Gas Chromatography Mass Spectrometry ◽  
Organic Residue ◽  
Organic Residues ◽  
Pyrolysis Gas ◽  
Main Component ◽  
Solids Analysis ◽  
Ceramic Casting

This research was focused on the analysis of material composition and organic residues present in three molds found in the Moravian region (Czech Republic) belonging to the Roman era. X-ray fluorescence spectroscopy pointed out the possible remelting of Roman objects in Barbarian territory. The analysis of organic residues retrieved from the internal part of mold #2 by pyrolysis-gas chromatography/mass spectrometry proved the presence of ozokerite wax (“earth wax”). Consequent analysis of this organic residue by Atmospheric Solids Analysis Probe–ion mobility spectrometry–high-resolution mass spectrometry (ASAP-IMS-HRMS) confirmed the presence of ceresin, the main component of ozokerite. Ceresin was also detected in a sample of the organic residue from mold #1. Note that this is the first application of ASAP-IMS-HRMS in archaeological research. The remains of earth wax in molds suggest the production of wax models as an intermediate stage for the production of lost-wax ceramic casting molds.

Proteome Analysis ofLactobacillus rhamnosusGG Using 2-D DIGE and Mass Spectrometry Shows Differential Protein Production in Laboratory and Industrial-Type Growth Media

2009 ◽  
Vol 8 (11) ◽  
pp. 4993-5007 ◽  
Author(s):  
Kerttu Koskenniemi ◽  
Johanna Koponen ◽  
Matti Kankainen ◽  
Kirsi Savijoki ◽  
Soile Tynkkynen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Production ◽  
Proteome Analysis ◽  
Growth Media ◽  
Differential Protein

Atmospheric Solids Analysis Probe Mass Spectrometry: A New Approach for Airborne Particle Analysis

2010 ◽  
Vol 82 (14) ◽  
pp. 5922-5927 ◽  
Author(s):  
Emily A. Bruns ◽  
Véronique Perraud ◽  
John Greaves ◽  
Barbara J. Finlayson-Pitts
Keyword(s):  
Mass Spectrometry ◽  
Particle Analysis ◽  
Airborne Particle ◽  
New Approach ◽  
Solids Analysis

Membrane Microdomain Structures of Liposomes and Their Contribution to the Cellular Uptake Efficiency into HeLa Cells

2016 ◽  
Vol 13 (2) ◽  
pp. 369-378 ◽  
Author(s):  
Yoshinori Onuki ◽  
Yasuko Obata ◽  
Kumi Kawano ◽  
Hiromu Sano ◽  
Reina Matsumoto ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Hela Cells ◽  
Uptake Efficiency ◽  
Membrane Microdomain ◽  
Cellular Uptake Efficiency

Evaluation of atmospheric solids analysis probe mass spectrometry for the analysis of coal-related model compounds

Fuel ◽  
2014 ◽  
Vol 117 ◽  
pp. 556-563 ◽  
Author(s):  
Shou-Ze Wang ◽  
Xing Fan ◽  
Ai-Li Zheng ◽  
Yu-Gao Wang ◽  
You-Quan Dou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Model Compounds ◽  
Related Model ◽  
Solids Analysis

Evaluation of sorghum root branching using fractals

1998 ◽  
Vol 131 (3) ◽  
pp. 259-265 ◽  
Author(s):  
C. E. A. MASI ◽  
J. W. MARANVILLE
Keyword(s):  
Fractal Dimension ◽  
Growth Media ◽  
Silty Clay ◽  
Root Branching ◽  
The Us ◽  
Branching Patterns ◽  
Novel Method ◽  
Sorghum Genotypes ◽  
Different Sources ◽  
Root Box

Root branching and architecture play a significant role in water and nutrient uptake, but description of these parameters has not been easy due to the difficulty of observing roots in their natural arrangement. Fractal geometry offers a novel method for studying the branching patterns of roots. Plants of ten diverse sorghum (Sorghum bicolor (L.) Moench) genotypes (five of African origin, three of US origin and two hybrids composed of African×US lines) were grown in root boxes containing 80% sand and 20% fine-textured Sharpsburg silty clay loam topsoil. The root fractal dimension (D) and abundance (log K) were determined at nine regions within the profile. Roots were washed free of growth media and photographic slides were taken of each region. Values of D and log K were determined by projecting photographs onto grids of progressively increasing sizes. The number of intersects was regressed on log grid size. Differences in D were found among genotypes (1·44[les ]D[les ]1·89) suggesting that these sorghum genotypes may be associated with greater root branching patterns. Greater fractal dimension (branching) and abundance values occurred in the 0–35 and 35–70 cm depths of the soil profile within the root box, indicating a greater root distribution in that part of the profile. Significant differences were also noted in branching patterns for sorghum genotypes derived from different sources. In general, the African sorghums were more branched and deeper rooted than the US-derived genotypes. Results indicated that fractal dimension can be used for the description of sorghum root system morphology and provides a good measure of branching patterns which can be distinguished.

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