Low-cost synthesis of carbon nanodots from millets for bioimaging

MRS Advances ◽  
2019 ◽  
Vol 4 (3-4) ◽  
pp. 249-254
Author(s):  
Ngo Khoa Quang ◽  
Che Thi Cam Ha
Keyword(s):  
Water Solubility ◽  
Biomedical Application ◽  
Low Cost ◽  
Average Diameter ◽  
Optical Measurements ◽  
Carbon Nanodots ◽  
Excitation Wavelength ◽  
Simple Method ◽  
Potential Applications ◽  
Multicolor Fluorescence

ABSTRACTWe presented a green and simple method to synthesize carbon nanodots (C-dots) from millets using hydrothermal synthesis route for the first time. The obtained C-dots have average diameter ranging from 6 to 10 nm. Optical measurements showed the insight into the formation of functional groups on the particle surfaces, resulting in their good water solubility and bioconjugation. After treatment with C-dots, small subpopulation of the human cervical tumor cells became bright and exhibited multicolor fluorescence under different excitation wavelength. The achievement demonstrated potential applications of fluorescent C-dots in the field of biomedical application.

scholarly journals ID: 1038 Hydrothermal synthesis of carbon nanodots from millets for cancer cells imaging

2017 ◽  
Vol 4 (S) ◽  
pp. 116
Author(s):  
Quang Ngo Khoa ◽  
Tran Thi Xuan Thuy ◽  
Che Thi Cam Ha
Keyword(s):  
Hydrothermal Synthesis ◽  
Cancer Cells ◽  
Biomedical Application ◽  
Average Diameter ◽  
Optical Measurements ◽  
Carbon Nanodots ◽  
Excitation Wavelength ◽  
Economical Method ◽  
Potential Applications ◽  
Multicolor Fluorescence

We presented a green, simple and economical method to synthesize carbon nanodots (C-dots) from millets using hydrothermal synthesis route. The obtained C-dots have average diameter ranging from 6 to 8 nm. Optical measurements showed the formation of hydroxyl, carbonyl/carboxyl, amino functional groups on the particle surfaces, resulting in their high hydrophilicity and bioconjugation. After treatment with C-dots, human cervical and lung cancer cells became bright and exhibited multicolor fluorescence under different excitation wavelength. The achievement demonstrated potential applications of fluorescent C-dots in the field of biomedical application, especially in diagnostic disease techniques.

scholarly journals Hydrothermal synthesis of carbon nanodots from wine cork and biocompatible fluorescent probe

2021 ◽  
Author(s):  
Ngo Khoa Quang ◽  
Nguyen Ngoc Hieu ◽  
Vo Van Quoc Bao ◽  
Vo Thi Phuoc ◽  
Le Xuan Diem Ngoc ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hydrothermal Synthesis ◽  
Low Cost ◽  
Average Diameter ◽  
Optical Measurements ◽  
Carbon Nanodots ◽  
Structural And Optical Properties ◽  
Simple Method ◽  
Fluorescent Image ◽  
Potential Applications

We presented a low-cost and simple method to synthesize carbon nanodots (CDs) from waste wine cork using hydrothermal synthesis.  The structural and optical properties of the CDs are characterized by TEM, FTIR, Raman, UV-Vis absorption, and photoluminescence (PL) spectra. The analysis results indicated the average diameter of CDs ⁓ 6.2 ± 2.7 nm. Optical measurements showed the phenomenon of excitation-dependent PL and the formation of functional groups on the surface of the particles. CDs with a quantum yield of 1.54% was calculated using quinine sulfate as reference. Furthermore, a probe of wine cork-derived CDs in bioimaging has been successfully applied in living mesenchymal stem cells (MSCs). After treatment with CDs, MSCs exhibited fluorescence including green, yellow, and red colors under the excitation wavelengths in the range 330–385 nm, 450–480 nm, and 510–550 nm, respectively. The achievement demonstrated potential applications of fluorescent CDs in the field of the fluorescent image.

scholarly journals Recent Findings in Azaphilone Pigments

2021 ◽  
Vol 7 (7) ◽  
pp. 541
Author(s):  
Lúcia P. S. Pimenta ◽  
Dhionne C. Gomes ◽  
Patrícia G. Cardoso ◽  
Jacqueline A. Takahashi
Keyword(s):  
Water Solubility ◽  
Biological Activities ◽  
Low Cost ◽  
Scale Up ◽  
Structural Diversity ◽  
Downstream Processing ◽  
Global Market ◽  
Yield Improvement ◽  
Potential Applications ◽  
New Compounds

Filamentous fungi are known to biosynthesize an extraordinary range of azaphilones pigments with structural diversity and advantages over vegetal-derived colored natural products such agile and simple cultivation in the lab, acceptance of low-cost substrates, speed yield improvement, and ease of downstream processing. Modern genetic engineering allows industrial production, providing pigments with higher thermostability, water-solubility, and promising bioactivities combined with ecological functions. This review, covering the literature from 2020 onwards, focuses on the state-of-the-art of azaphilone dyes, the global market scenario, new compounds isolated in the period with respective biological activities, and biosynthetic pathways. Furthermore, we discussed the innovations of azaphilone cultivation and extraction techniques, as well as in yield improvement and scale-up. Potential applications in the food, cosmetic, pharmaceutical, and textile industries were also explored.

A carbon-based nanocarrier for efficient gene delivery

2021 ◽  
Author(s):  
Tahereh Kashkoulinejad-Kouhi ◽  
Shadi Sawalha ◽  
Shahrokh Safarian ◽  
Blanca Arnaiz
Keyword(s):  
Hydrogen Peroxide ◽  
Gene Delivery ◽  
Water Solubility ◽  
Chemotherapy Resistance ◽  
A549 Cells ◽  
Carbon Nanodots ◽  
Simple Method ◽  
Efficient Gene ◽  
Low Toxicity ◽  
Positively Charged

Aim: Several types of nanocarriers, most of which show significant cytotoxicity, have been developed to overcome the problem of gene-delivery barriers. Biocompatibility, low toxicity and water solubility of carbon nanodots (CNDs) are major advantages that recommend them as delivery systems. Materials & methods: We present a simple method to produce positively charged CNDs. Ethanolamine, ethylenediamine and hydrogen peroxide were utilized to synthesize these CNDs. Results & conclusion: Our results indicated that delivery of the CND–siGFP complex led to significant switching-off of the fluorescence of the GFP-expressing A549 cell. Next, the A549 cells were transfected with siRNA against BiP, which is a pivotal protein in the chemotherapy resistance of cancer cells. The expression levels of BiP decreased remarkably.

L-Histidine Stabilized Copper Nanoclusters as a Fluorescence Probe for Determination of Fluazinam

NANO ◽  
2020 ◽  
Vol 15 (05) ◽  
pp. 2050063
Author(s):  
Zhifeng Cai ◽  
Xiu Yin ◽  
Jingling Fang ◽  
Jie Zhao ◽  
Tianqi Wu ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Photoelectron Spectroscopy ◽  
Water Solubility ◽  
Fluorescence Probe ◽  
Synthesis Method ◽  
Fluorescence Emission ◽  
Average Diameter ◽  
High Dispersion ◽  
Excitation Wavelength ◽  
One Pot

In this contribution, a one-pot synthesis method possessing the advantages of simple, green and low-cost had been researched for the preparation of L-histidine-stabilized Cu nanoclusters (Cu NCs). Subsequently, the structure and optical properties of as-prepared Cu NCs were studied by using Fourier transform infrared (FTIR), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), fluorescence spectroscopy and UV-Vis absorption spectroscopy. TEM image of the Cu NCs showed high dispersion with an average diameter of 2.0[Formula: see text]nm. Fluorescence spectrum displayed that the Cu NCs emitted green fluorescence (emission wavelength of 492[Formula: see text]nm) under excitation wavelength of 393[Formula: see text]nm. Moreover, the as-synthesized Cu NCs illustrated excellent performances, such as good water solubility, UV stability and high-salt resistance. Interestingly, the fluorescence intensity of as-prepared Cu NCs was obviously quenched in the presence of fluazinam. Under optimal conditions, the relative fluorescence intensity was linear with the fluazinam concentrations from 1 to 40[Formula: see text][Formula: see text]M, with a detection limit of 0.25[Formula: see text][Formula: see text]M. Eventually, the fluorescence sensor was successfully used to determine fluazinam in real water samples.

scholarly journals Tribological Anti-Wear and Extreme-Pressure Performance of Multifunctional Metal and Nonmetal Doped C-based Nanodots

Lubricants ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 36 ◽  
Author(s):  
Tomala ◽  
Kumar ◽  
Porat ◽  
Michalczewski ◽  
Gedanken
Keyword(s):  
Tribological Properties ◽  
Water Solubility ◽  
Low Cost ◽  
Carbon Nanodots ◽  
Extreme Pressure ◽  
One Pot ◽  
Engine Oils ◽  
Nitrogen Doped ◽  
Tribological Study ◽  
First Time

Carbon nanodots (CDs) are extensively explored due to their little toxicity, excellent water solubility, and biocompatibility. Particularly, fluorescent CDs have received ever-increasing attention. Nevertheless, up to now, only a few findings have been dedicated to measuring the tribological properties of doped CDs, especially Ga doped CDs (Ga@CDs and nitrogen doped CDs (N@CDs)), and to compare their tribological properties with CDs. Here, we describe a strategy for the low-cost one-pot synthesis of CDs for tribological study. The presented research for the first time describes tribological properties and indicates a possible application of the multifunctional CDs (N@CDs, Ga@CDs, and CDs) as highly specific materials for various tests of engine oils and hydraulic oils. Further, it is stated that the doping of the CDs with various elements can tailor demanding tribological performances like anti-wear and extreme-pressure performances.

scholarly journals DFT Based Material Computations of Metal-doped and Pure Carbon Nanodots for Examining their Enhancement of Sulfur Dioxide Adsorption

2021 ◽  
Author(s):  
Mikail ASLAN
Keyword(s):  
Low Cost ◽  
Carbon Nanodots ◽  
High Adsorption ◽  
Impurity Atoms ◽  
So2 Adsorption ◽  
Gas Molecule ◽  
Pure Carbon ◽  
Potential Applications ◽  
Metal Doped ◽  
So2 Gas

Carbon nanodots, one of the last members of the nanocarbon family, show superior properties, such as low-cost production, good conductivity, and optical properties, nontoxic behavior, high biocompatibility, and eco-friendly nature. Understanding the effect of metal doping on the modification of the electronic structure of carbon nanodots is critical for enlarging its potential applications. In the present study, in terms of structural, energetic, and electronic analyses, X-doped carbon nanodot structures (X = B, N, Si, Al, Co, Au, Pd, and Pt) and their SO2 adsorption abilities were examined comprehensively by employing DFT. Results depict that embedding the heavy impurity metals (Pd, Pt) to the nanodot structures does not improve the SO2 sensing ability of carbon nanodot materials relatively. However, the doping of the low concentrated metals to the carbon nanodots may be one of the best ways for enhancing the SO2 trapping ability of the carbon nanodot materials since the calculated results having high adsorption energy values indicate SO2 gas molecule is easily adsorbed on the surface of doped carbon nanodots. This means higher adsorption capability compared to pure ones. Thus, it is suggested that the doped carbon nanodots consisting of B, Si, and N impurity atoms may be good candidates for effective SO2 sensing (adsorptions).

Carbon Dots as Nanotherapeutics for Biomedical Application

2020 ◽  
Vol 26 (19) ◽  
pp. 2207-2221 ◽  
Author(s):  
Eemaan N. Cohen ◽  
Pierre P.D. Kondiah ◽  
Yahya E. Choonara ◽  
Lisa C. du Toit ◽  
Viness Pillay
Keyword(s):  
Drug Delivery ◽  
Side Effects ◽  
Targeted Drug Delivery ◽  
Carbon Dots ◽  
Water Solubility ◽  
Biomedical Application ◽  
Drug Efficacy ◽  
Cost Effective ◽  
Carbon Nanodots ◽  
Targeted Drug

Carbon nanodots are zero-dimensional spherical allotropes of carbon and are less than 10nm in size (ranging from 2-8nm). Based on their biocompatibility, remarkable water solubility, eco- friendliness, conductivity, desirable optical properties and low toxicity, carbon dots have revolutionized the biomedical field. In addition, they have intrinsic photo-luminesce to facilitate bio-imaging, bio-sensing and theranostics. Carbon dots are also ideal for targeted drug delivery. Through functionalization of their surfaces for attachment of receptor-specific ligands, they ultimately result in improved drug efficacy and a decrease in side-effects. This feature may be ideal for effective chemo-, gene- and antibiotic-therapy. Carbon dots also comply with green chemistry principles with regard to their safe, rapid and eco-friendly synthesis. Carbon dots thus, have significantly enhanced drug delivery and exhibit much promise for future biomedical applications. The purpose of this review is to elucidate the various applications of carbon dots in biomedical fields. In doing so, this review highlights the synthesis, surface functionalization and applicability of biodegradable polymers for the synthesis of carbon dots. It further highlights a myriad of biodegradable, biocompatible and cost-effective polymers that can be utilized for the fabrication of carbon dots. The limitations of these polymers are illustrated as well. Additionally, this review discusses the application of carbon dots in theranostics, chemo-sensing and targeted drug delivery systems. This review also serves to discuss the various properties of carbon dots which allow chemotherapy and gene therapy to be safer and more target-specific, resulting in the reduction of side effects experienced by patients and also the overall increase in patient compliance and quality of life.

Effect of Ultrasonic Amplitude on Surface Properties of Anodised Titanium for Biomedical Application

2016 ◽  
Vol 840 ◽  
pp. 160-164 ◽  
Author(s):  
Te Chuan Lee ◽  
Mohd Hafifi Hafizat Mazlan ◽  
Mohamad Imran Abbas ◽  
Hasan Zuhudi Abdullah ◽  
Maizlinda Izwana Idris
Keyword(s):  
Surface Properties ◽  
Biomedical Application ◽  
Low Cost ◽  
Disodium Salt ◽  
Anode Surface ◽  
Simple Method ◽  
Force Microscopy ◽  
Atomic Force ◽  
Ceramic Films ◽  
Titanium Foils

Anodic oxidation is an electrochemical method for the production of ceramic films on a metallic substrate. It is a simple and low cost method to produce bioactive material. This work describes the effect of ultrasonic amplitude on the surface properties of anodised titanium. Specifically, high purity titanium foils were anodised in mixture of 0.04 M β-glycerophosphate disodium salt pentahydrate (β-GP) and 0.4 M calcium acetate monohydrate (CA) at 350 V and 70 mA.cm-2 for 10 minutes. The ultrasonic amplitude was varied from 20-60 μm. Next, field emission scanning electron microscopy (FESEM) glancing angle X-ray diffractometer (GAXRD) and atomic force microscopy (AFM) were used to characterise the anodised titanium. The results showed that application of sonication is able to remove the entrapped bubbles on the anode surface and enhance the oxidation process. The pores size and surface roughness were increased as increasing of ultrasonic amplitude. At ultrasonic amplitude ≥ 50 μm, rutile TiO2 was formed on the surface of oxide layer. It was found that the sonication is a simple method to improve the surface properties of anodised titanium for implant applications.

A Simple and Rapid Phosphorescence Probe Based on Mn-Doped ZnS Quantum Dots for Chloramphenicol Detection

NANO ◽  
2019 ◽  
Vol 14 (11) ◽  
pp. 1950144
Author(s):  
Zhenping Liu ◽  
Xianfeng Wang ◽  
Jingzhou Hou ◽  
Danqun Huo ◽  
Changjun Hou
Keyword(s):  
Quantum Dots ◽  
Low Cost ◽  
Inner Filter Effect ◽  
Average Diameter ◽  
Excitation Wavelength ◽  
Optimal Conditions ◽  
Phosphorescence Probe ◽  
Cap Analysis ◽  
Mn Doped ◽  
Nanometer Particle

An innovative phosphorescence probe based on Mn-doped ZnS quantum dots (Mn:ZnS QDs) was developed for selective detection of chloramphenicol (CAP) via inner-filter effect (IFE). Mn:ZnS QDs were synthesized by water method and modified with L-Cysteine for better stability, and the average diameter of the nanometer particle was 3.8[Formula: see text]nm. With the excitation wavelength at 289[Formula: see text]nm, the strong phosphorescence of Mn:ZnS QDs can be emitted at 583[Formula: see text]nm. The excitation spectrum of Mn:ZnS QDs was substantially overlapped with the absorption spectrum of the target CAP. The excited light of Mn:ZnS QDs can be absorbed partially by CAP when they coexist, the phosphorescence intensity decreased with the increasing concentration of CAP, and it has a good linear relationship. Under optimal conditions, the linear relational concentration range achieved four orders of magnitude from 25 to [Formula: see text] ([Formula: see text]), with a detection limit (LOD; [Formula: see text]) down to 0.81[Formula: see text][Formula: see text]. The simple, rapid and low cost IFE phosphorescent probe exhibited satisfactory recoveries ranging from 88.9% to 98.5% for CAP analysis in spiked honey, which shows a potential for routine screening of CAP in ensuring the food safety.

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