Kinetics for De-Extracellular Matrix(ECM)

MRS Advances ◽  
2018 ◽  
Vol 3 (40) ◽  
pp. 2379-2384 ◽  
Author(s):  
Namsoo P. Kim ◽  
Jihye Kim ◽  
Guikuan Yue ◽  
Diana Cho
Keyword(s):  
Mass Transfer ◽  
Extracellular Matrix ◽  
Deoxyribonucleic Acid ◽  
Deoxycholic Acid ◽  
Reaction Order ◽  
Heterogeneous Reaction ◽  
Transfer Coefficients ◽  
Batch System ◽  
Solid Liquid Interface ◽  
Solid Liquid

ABSTRACTA kinetic model for ECM decellularization with Trypsin and Deoxycholic Acid as reactants in a batch system has been developed. The decellularization mechanism has been analysed by solving a series of ordinary differential equations relating heterogeneous reaction kinetics at the solid/liquid interface to mass transfer of reactant and products. In order to remove the Deoxyribonucleic acid (DNA) of porcine, special emphasis has been given to the surface area for regulating the depth and amount of the penetration of the reagent, the numerical analysis thereof, and the corresponding experiment were carried out. The effect of chemical reaction order, rate constants and mass transfer coefficients on the overall decellularization rate has been analysed and discussed.

The role of (bio)surfactant sorption in promoting the bioavailability of nutrients localized at the solid-water interface

1999 ◽  
Vol 39 (7) ◽  
pp. 91-98 ◽  
Author(s):  
Ryan N. Jordan ◽  
Eric P. Nichols ◽  
Alfred B. Cunningham
Keyword(s):  
Mass Transfer ◽  
Cell Membrane ◽  
Substrate Concentration ◽  
Water Interface ◽  
Industrial Systems ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Surfactant Sorption

Bioavailability is herein defined as the accessibility of a substrate by a microorganism. Further, bioavailability is governed by (1) the substrate concentration that the cell membrane “sees,” (i.e., the “directly bioavailable” pool) as well as (2) the rate of mass transfer from potentially bioavailable (e.g., nonaqueous) phases to the directly bioavailable (e.g., aqueous) phase. Mechanisms by which sorbed (bio)surfactants influence these two processes are discussed. We propose the hypothesis that the sorption of (bio)surfactants at the solid-liquid interface is partially responsible for the increased bioavailability of surface-bound nutrients, and offer this as a basis for suggesting the development of engineered in-situ bioremediation technologies that take advantage of low (bio)surfactant concentrations. In addition, other industrial systems where bioavailability phenomena should be considered are addressed.

Double channel electrodes and the measurement of heterogeneous reaction rates at the solid-liquid interface

1988 ◽  
Vol 18 (4) ◽  
pp. 657-665 ◽  
Author(s):  
Richard G. Compton ◽  
Geoffrey M. Stearn ◽  
Patrick R. Unwin ◽  
Anthony J. Barwise
Keyword(s):  
Heterogeneous Reaction ◽  
Reaction Rates ◽  
Liquid Interface ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Double Channel
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