Lysine-Capped Silica Nanoparticles: A Solid-State NMR Spectroscopy Study

MRS Advances ◽  
2016 ◽  
Vol 1 (31) ◽  
pp. 2261-2266 ◽  
Author(s):  
Chengchen Guo ◽  
Gregory P. Holland ◽  
Jeffery L. Yarger
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Silica Nanoparticles ◽  
Protein Interactions ◽  
Solid State Nmr ◽  
Silanol Group ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Solid State Nmr Spectroscopy ◽  
Fumed Silica Nanoparticles

ABSTRACTTo achieve the goal of biocompatibility in nano-based materials we must first obtain a fundamental understanding of the physical and chemical behavior of biomolecules at the interfaces of nanomaterials. A first step towards understanding protein interactions with nanomaterials is to understand how individual amino acids interact at the interfaces. In this paper, we investigated the lysine adsorption behavior on fumed silica nanoparticles by solid-state NMR spectroscopy. We use 1H, 13C and 15N solid-state magic angle spinning (MAS) NMR techniques to elucidate how lysine is adsorbed on silica nanoparticles surfaces via strong hydrogen-bonding interaction between the protonated side-chain amine group and silanol group on silica nanoparticles surfaces.*

scholarly journals Characterization of nucleosome sediments for protein interaction studies by solid-state NMR spectroscopy

2021 ◽  
Author(s):  
Ulric B. le Paige ◽  
ShengQi Xiang ◽  
Marco M. R. M. Hendrix ◽  
Yi Zhang ◽  
Markus Weingarth ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Solid State Nmr ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Dense Phase ◽  
Binding Studies ◽  
Nmr Studies ◽  
Solid State Nmr Spectroscopy ◽  
Wide Range

Abstract. Regulation of DNA-templated processes such as gene transcription and DNA repair depend on the interaction of a wide range of proteins to the nucleosome, the fundamental building block of chromatin. Both solution and solid-state NMR spectroscopy have become an attractive approach to study the dynamics and interactions of nucleosomes, despite their high molecular weight of ~200 kDa. For solid-state NMR (ssNMR) studies, dilute solutions of nucleosomes are converted to a dense phase by sedimentation or precipitation. Since nucleosomes are known to self-associate, these dense phases may induce extensive interactions between nucleosomes, which could interfere with protein binding studies. Here, we characterized the packing of nucleosomes in the dense phase created by sedimentation using NMR and small-angle x-ray scattering (SAXS) experiments. We found that nucleosome sediments are gels with variable degrees of solidity, have nucleosome concentration close to that found in crystals, and are stable for weeks under high-speed magic angle spinning (MAS). Furthermore, SAXS data recorded on recovered sediments indicate that there is no pronounced long-range ordering of nucleosomes in the sediment. Finally, we show that the sedimentation approach can also be used to study low affinity protein interactions with the nucleosome. Together, our results give new insights into the sample characteristics of nucleosome sediments for ssNMR studies and illustrate the broad applicability of sedimentation-based NMR studies.

A unified heteronuclear decoupling strategy for magic-angle-spinning solid-state NMR spectroscopy

2015 ◽  
Vol 142 (18) ◽  
pp. 184201 ◽  
Author(s):  
Asif Equbal ◽  
Morten Bjerring ◽  
P. K. Madhu ◽  
Niels Chr. Nielsen
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Solid State Nmr ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Solid State Nmr Spectroscopy ◽  
Angle Spinning

scholarly journals Solid-state NMR measurements and DFT calculations of the magnetic shielding tensors of protons of water trapped in barium chlorate monohydrate

RSC Advances ◽  
2014 ◽  
Vol 4 (99) ◽  
pp. 56248-56258 ◽  
Author(s):  
Diego Carnevale ◽  
Sharon E. Ashbrook ◽  
Geoffrey Bodenhausen
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Dft Calculations ◽  
Solid State Nmr ◽  
Magic Angle Spinning ◽  
Magnetic Shielding ◽  
Magic Angle ◽  
Solid State Nmr Spectroscopy ◽  
Shielding Tensors ◽  
Angle Spinning

The magnetic shielding tensors of protons of water in barium chlorate monohydrate are investigated by means of solid-state NMR spectroscopy, both for static powders and under magic-angle spinning conditions.

Intermolecular Protein−RNA Interactions Revealed by 2D31P−15N Magic Angle Spinning Solid-State NMR Spectroscopy

2010 ◽  
Vol 132 (11) ◽  
pp. 3842-3846 ◽  
Author(s):  
Stefan Jehle ◽  
Melanie Falb ◽  
John P. Kirkpatrick ◽  
Hartmut Oschkinat ◽  
Barth-Jan van Rossum ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Solid State Nmr ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Solid State Nmr Spectroscopy ◽  
Angle Spinning
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