A rapid validated high-performance thin-layer chromatographic-densitometric method for the simultaneous estimation of different chemical-nature compounds piperine and gallic acid in pharmaceutical dosage forms

2013 ◽  
Vol 26 (4) ◽  
pp. 325-330 ◽  
Author(s):  
Subrata De ◽  
Pankaj Nariya ◽  
Nikhil Jirankalgikar
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
High Performance ◽  
Chemical Nature ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Forms

HPTLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CHLORTHALIDONE AND IRBESARTAN IN COMBINED TABLET DOSAGE FORM

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (07) ◽  
pp. 46-52
Author(s):  
P Kalaiselvi ◽  
◽  
K. G. Lalitha
Keyword(s):  
High Performance ◽  
Method Development ◽  
Limit Of Detection ◽  
Ammonia Solution ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Limit Of Quantification ◽  
Pharmaceutical Dosage Forms ◽  
Tablet Dosage ◽  
Hptlc Method

A simple, accurate and rapid high performance thin layer chromatography (HPTLC)-densitometry method was developed for separation and determination of chlorthalidone (CHL) and irbesartan (IBS) in pharmaceutical dosage forms. The compounds were separated on silica gel 60 GF254, HPTLC plates using mobile phase of toluene: ethyl acetate: acetonitrile: methanol: ammonia solution (25%) [5:2:2:1:0.2 v/v/v/v] as compact spots at Rf of 0.57 for chlorthalidone and Rf of 0.36 for irbesartan. Densitometric detection was performed at 254 nm. The method was validated in terms of linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ). The calibration curves were linear in the range of 12.5-75 ng/spot for CHL and 150-900ng/spot for IBS. For CHL recovery varied in range of 99.26-101.25% and for IBS recovery varied in range of 99.76-100.40%. The LOD was found 1.33 and 11.34 ng/spot for CHL and IBS respectively. The LOQ was found 4.03 and 14.37 ng /spot for CHL and IBS respectively. It was observed that the proposed HPTLC method could be used for efficient analysis of the CHL and IBS in combined tablet dosage forms.

scholarly journals Simultaneous estimation of phenylephrine HCl, chlorpheniramine maleate and dextromethorphan HBr in a pharmaceutical (syrup) formulations by RP-HPLC using PDA detector

2016 ◽  
Vol 2 (2) ◽  
pp. 89 ◽  
Author(s):  
S Ashutosh Kumar ◽  
Manidipa Debnath ◽  
D. Vimala
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Dosage Forms ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Chlorpheniramine Maleate ◽  
Pharmaceutical Dosage Forms ◽  
Bulk Drug ◽  
Tablet Dosage

The present study aimed to develop and validate the simultaneous estimation of phenylephrine HCl, chlorpheniramine maleate and dextromethorphan HBr in tablet dosage forms. A gradient reversed phase high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 220 nm has been developed for the simulataneous determination of phenylephrine HCl, chlorpheniramine maleate and dextromethorphan HBr in pharmaceutical dosage forms (Syrup). Good chromatographic separation was achieved by using a stainless steel analytical column, the Hypersil BDS C8 column (4.6 X 250 mm; 5 μm). The system was operated at 25 ± 2°C using a mobile phase consisted of HPLC grade water (composed of TEA and 1-octane sulfonic acid sodium salt) (pH adjusted to 3.2 using orthophosphoric acid) and acetonitrile, mixed at gradient mode, manitained flow rate at 1.0 mL/minute. The slope, intercept, and correlation coefficient were found to be y = 34306x - 11042 (r2= 0.999) for phenylephrine HCl, y = 35874x - 13101 (r2= 0.999) for chlorpheniramine maleate and dextromethorphan HBr y = 25516x - 26579 (r2 = 0.999), respectively. The proposed method was validated for its specificity, linearity, accuracy, and precision. The method was found to be suitable for the quality control of phenylephrine HCl, chlorpheniramine maleate and dextromethorphan HBr simultaneously in a bulk drug samples as well as in a formulations.

scholarly journals Gas and high performance thin layer chromatographic based-determination method for quantification of thymol in semisolid traditional dosage forms

2020 ◽  
Vol 10 (2) ◽  
pp. 4982-4987
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
High Performance ◽  
Dosage Forms ◽  
Lepidium Sativum ◽  
Pharmaceutical Dosage Forms ◽  
Content Determination ◽  
Validation Parameters ◽  
Persian Medicine ◽  
Traditional Persian Medicine

The control and standardization process of herbal products is a critical point in preparation of those medicaments. In Traditional Persian Medicine (TPM) literature, out of all the different pharmaceutical dosage forms, Jawarish is a semisolid gastrointestinal dosage form with positive related effects. Jawarish-e-Khuzi, including Zataria multiflora, Lepidium sativum, Trachyspermum ammi, Terminalia chebula, ferrous sulfate, and also honey is one of the popular mentioned traditional oral formulations. However, there have been no noticeable and proven control and standardization for this formulation. In this study, Jawarish-e-Khuzi was prepared based on one of the pharmaceutical textbooks of Traditional Persian Medicine (TPM). Using gas chromatography/mass spectroscopy (GC/MS), the volatile composition of this formulation was analyzed. Subsequently, Gas chromatography/flame ionization detector (GC/FID) and High-Performance Thin-Layer Chromatography (HPTLC) techniques were employed to determine the main component. The GC/MS results showed thymol as the main constituent. In the content determination process via GC/FID, thymol was proved to be 0.02% of the whole preparation. The outcome of HPTLC method also corresponded with that of GC/FID. Based on the method validation parameters, both GC/FID and HPTLC methods are useful for the volatile content determination of semisolid dosage forms.

scholarly journals SIMULTANEOUS ESTIMATION OF CURCUMINOIDS, PIPERINE, AND GALLIC ACID IN AN AYURVEDIC FORMULATION BY VALIDATED HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD

2016 ◽  
pp. 117
Author(s):  
Niraj Yashvantrai Vyas ◽  
Sejal Patel
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
High Performance ◽  
Retention Factor ◽  
Simultaneous Estimation ◽  
Phase Plate ◽  
Analytical Work ◽  
Ambient Room Temperature ◽  
Marker Compounds ◽  
Hptlc Method

<p>ABSTRACT<br />Objective: The present study was proposed to quantitatively estimate the amount of three marker compounds; curcuminoids, piperine, and gallic<br />acid in a multicomponent ayurvedic formulation using high-performance thin layer chromatographic (HPTLC) method for routine analytical work.<br />Methods: TLC separation was performed on silica gel 60 F<br /> plates using toluene:ethyl acetate:formic acid:methanol (5.6:2.2:1.2:1.0 v/v/v/v) as<br />mobile phase. Plate was developed by to a distance of 90 mm at ambient room temperature with 20 minutes saturation time. Densitometric analysis<br />was performed at 327 nm. Method was validated as per International Conference on Harmonization Q2 (R1) guideline also.<br />254<br />Results: Piperine, curcuminoids, and gallic acid were separated on TLC at retention factor values of 0.71, 0.61, and 0.29, respectively. The described<br />method was linear over the range of 300-700 ng/spot, 100-300 ng/spot, and 250-550 ng/spot, respectively, for curcuminoids, piperine, and gallic<br />acid. The accuracy of the method was assessed by recovery studies and was found to be 101.71%, 99.67%, and 99.59% for curcuminoids, piperine,<br />and gallic acid, respectively. The amount of curcuminoids, piperine, and gallic acid in the ayurvedic formulation was found to be 3.99% w/w, 1.9%<br />w/w, and 0.8% w/w, respectively, when analyzed quantitatively by developed validated HPTLC method.<br />Conclusion: The method can be used as a tool for quality control of herbal formulation.<br />Keywords: Curcuminoids, Piperine, Gallic acid, High-performance thin layer chromatographic.</p>

scholarly journals Simultaneous Estimation of Racecadotril and Ofloxacin by Reverse Phase High Performance Liquid Chromatography Method in Pharmaceutical Dosage Forms.

2019 ◽  
Vol 9 (4-s) ◽  
pp. 165-170
Author(s):  
Komal Gupta ◽  
Deepika Sharma ◽  
Pooja Chawla
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Chromatography Method ◽  
Pharmaceutical Dosage Forms ◽  
Ich Guidelines ◽  
Liquid Chromatography Method

Background: Racecadotril and Ofloxacin (RACIGYL- O) drug combination are used for the treatment of diarrhea. The drugs have been estimated individually in formulations but no method has been developed for simultaneous estimation of these two drugs as combination. Objective: To develop and validate a high performance liquid chromatography method for the simultaneous estimation of Racecadotril and Ofloxacin in tablet dosage form. Method: A WATERS C18 column (250 x 4.6mm, 5 μm) with mobile phase consisting of acetonitrile, methanol and water 40:40:20v/v (pH adjusted to 2.7 of water with ortho phosphoric acid). The flow rate was 1.0mL/min and effluents were monitored at 210nm. Results: The retention time of RAC and OFL was 4.666 min and 2.551 min respectively. The developed method was validated according to ICH guidelines with respect to specificity, linearity, accuracy, precision, robustness, Limit of Detection (LOD) and Limit of Quantification (LOQ). Conclusion: The method show good reproducibility and recovery with %RSD less than 2. So the proposed method was found to be simple, rapid, precise and accurate and useful for the determination of RAC and OFL in bulk and pharmaceutical dosage forms.   Keywords: RP-HPLC, Racecadotril, Ofloxacin, ICH guidelines.

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