scholarly journals Quantitative two-dimensional thin-layer chromatography using a diode-array detector

2012 ◽  
Vol 25 (6) ◽  
pp. 493-497 ◽  
Author(s):  
Barbara Milz ◽  
Karl-Friedrich Klein ◽  
Bernd Spangenberg
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Array Detector ◽  
Diode Array ◽  
Two Dimensional ◽  
Diode Array Detector ◽  
Layer Chromatography

Determination of Ibuprofen and Phenylephrine in Tablets by High-Performance Thin Layer Chromatography and in Plasma by High-Performance Liquid Chromatography with Diode Array Detection

2019 ◽  
Vol 57 (7) ◽  
pp. 592-599
Author(s):  
Marwa A A Ragab ◽  
Mohamed H Abdel-Hay ◽  
Hytham M Ahmed ◽  
Sara M Mohyeldin
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biological Fluids ◽  
Array Detector ◽  
Diode Array ◽  
Simultaneous Extraction ◽  
Layer Chromatography

Abstract Two chromatographic methods (high performance thin layer chromatography (HPTLC) and high performance liquid chromatography–diode array detector (HPLC-DAD)), were addressed for the analysis of a mixture consisted of phenylephrine hydrochloride and ibuprofen in two forms bulk and their combined dosage form. This binary mixture is considered to be a challenging one as the two drugs differ greatly in their chemical and physical properties. Not only this affects their simultaneous analysis, but also hinders their simultaneous extraction from biological fluids as plasma. That is the reason the literature lacks any report for the simultaneous extraction and analysis of these drugs from biological fluids. The concentration ranges of both drugs were 0.1–2.5 μg/spot and 0.1–100 μg/mL by HPTLC and HPLC, respectively. Not only was the HPLC-DAD method applied to the investigated drugs determination in pharmaceutical preparations, but also in spiked human plasma. Extensive study was conducted to optimize their simultaneous extraction from plasma as it was a crucial step for the in vivo analysis. The results obtained by proposed methods and a reference one were statistically comparable by analysis of variance test. No significant difference was recorded between the mean percent levels determined by the proposed methods and the reference one.

scholarly journals Determination of Pesticides in Water Samples from the Wieprz-Krzna Canal in the czysko-Wodawskie Lake District of Southeastern Poland by Thin-Layer Chromatography with Diode Array Scanning and High-Performance Column Liquid Chromatography with Diode Array Detection

2008 ◽  
Vol 91 (5) ◽  
pp. 1203-1209 ◽  
Author(s):  
Tomasz Tuzimski
Keyword(s):  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Water Samples ◽  
High Performance ◽  
Limit Of Detection ◽  
Array Detector ◽  
Diode Array ◽  
Layer Chromatography

Abstract High-performance thin-layer chromatography with diode array scanning (TLC-DAD) and high-performance column liquid chromatography with a diode array detector (HPLC-DAD) were used to screen water samples for pesticides. Atrazine, clofentezine, chlorfenvinphos, hexaflumuron, terbuthylazine, lenacyl, neburon, bitertanol, and metamitron were enriched from canal water samples by solid-phase extraction (SPE) on octadecyl silane (C18)/styrene-divinylbenzene-1, C18, C18 Polar Plus, and cyanopropyl (CN) cartridges. Recovery rates were high for all extraction materials except CN, for which values for all pesticides were lower. SPE was used for both preconcentration and fractionation of the analytes. Analytes were eluted by means of methanol and dichloromethane. Methanol eluates were analyzed by HPLC-DAD and dichloromethane eluates by TLC-DAD. The method was validated for precision, repeatability, and accuracy. Calibration graphs were linear between 0.1 and 50.0 g/mL for all pesticides, and correlation coefficient (r) values were between 0.9994 and 1.000 as determined by HPLC-DAD. Calibration graphs were linear between 0.1 and 1.5 g/spot for all pesticides, and r values were between 0.9899 and 0.9987 as determined by TLC-DAD. The limit of detection was between 0.04 and 0.23 g/spot for TLC-DAD and 0.02 and 0.45 g/mL for HPLC-DAD.

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