Development and validation of an HPTLC method for the simultaneous determination of diosmin and hesperidin in different citrus fruit extracts and pharmaceutical formulations

2012 ◽  
Vol 25 (2) ◽  
pp. 138-144 ◽  
Author(s):  
Eman Shawky
Keyword(s):  
Simultaneous Determination ◽  
Citrus Fruit ◽  
Pharmaceutical Formulations ◽  
Fruit Extracts ◽  
Development And Validation ◽  
Hptlc Method

scholarly journals A Novel UPLC-PDA Stability Indicating Method Development and Validation for the Simultaneous Estimation of Lamivudine and Dolutegravir in Bulk and Its Tablets

2020 ◽  
pp. 52-60
Author(s):  
Poojari Venkatesh ◽  
Umasankar Kulandaivelu ◽  
Gsn Koteswara Rao ◽  
Guntupalli Chakravarthi ◽  
Rajasekhar Reddy Alavala ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Method Development ◽  
Pharmaceutical Formulations ◽  
Simultaneous Estimation ◽  
Stability Indicating ◽  
Stability Indicating Method ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Potassium Dihydrogen Orthophosphate

Aim: To develop a stability indicating Rp-UPLC method for the simultaneous determination of Lamivudine, Dolutegravir and their degradants in tablets. Methodology: The chromatographic separation was performed on BEH Shield RP18 (2.1 mmX100 mmX1.7 mm) using a isocratic mobile phase Potassium dihydrogen orthophosphate pH 3 adjusted with orthophosphoric acid: methanol (30:70,% v/v) at a flow rate of 0.5ml/min. Column was maintained at room temperature and eluents are monitored at 258 nm. Results: Retention times of the analytes were found to be at 0.81 and 2.78 mins for Lamivudine and Dolutegravir respectively. The calibration of peak area versus concentration, which was linear from 105 to 315 µg/ml for Lamivudine and 17.5 to 52.5 µg/ml for Dolutegravir, had regression coefficient (r2) greater than 0.999. The method had the requisite accuracy, precision and robustness for simultaneous determination of Lamivudine and Dolutegravir in tablets. Conclusion: The proposed method is simple, economical, accurate, precise and can be successfully employed in routine quality control for the simultaneous analysis of Lamivudine and Dolutegravir in pharmaceutical formulations.

scholarly journals Validated HPTLC method for the simultaneous determination of alfuzosin, terazosin, prazosin, doxazosin and finasteride in pharmaceutical formulations

2014 ◽  
Vol 1 ◽  
pp. 23-31 ◽  
Author(s):  
Tarek S. Belal ◽  
Mohamed S. Mahrous ◽  
Magdi M. Abdel-Khalek ◽  
Hoda G. Daabees ◽  
Mona M. Khamis
Keyword(s):  
Simultaneous Determination ◽  
Pharmaceutical Formulations ◽  
Hptlc Method

scholarly journals Development and Validation of an HPTLC Method for Simultaneous Quantitation of Isoorientin, Isovitexin, Orientin, and Vitexin in Bamboo-Leaf Flavonoids

2010 ◽  
Vol 93 (5) ◽  
pp. 1376-1383 ◽  
Author(s):  
Jin Wang ◽  
Feng Tang ◽  
Yongde Yue ◽  
Xuefeng Guo ◽  
Xi Yao
Keyword(s):  
Simultaneous Determination ◽  
Silica Gel ◽  
Mobile Phase ◽  
Correlation Coefficients ◽  
Average Recovery ◽  
Recovery Study ◽  
Development And Validation ◽  
Hptlc Method ◽  
Different Levels

Abstract A simple HPTLC method has been developed for the simultaneous determination of isoorientin, isovitexin, orientin, and vitexin, both pure and in commercial samples of bamboo-leaf flavonoids. The flavone C-glycosides, including isoorientin, isovitexin, orientin, and vitexin, were extracted from bamboo-leaf flavonoids with methanol and chromatographed on silica gel 60 plates in an automatic developing chamber with tetrahydrofurantolueneformic acidwater (16 8 2 1, v/v/v/v) mobile phase. Quantitation was obtained with UV detection at 350 nm. Polynomial calibration plots were constructed in the concentration range 2001200 ng/zone for isoorientin, 100600 ng/zone for isovitexin, 160960 ng/zone for orientin, and 30360 ng/zone for vitexin with good correlation coefficients (r ≥ 0.9995). The method was validated for precision (interday and intraday), repeatability, and accuracy. Accuracy of the method was evaluated by a recovery study conducted at three different levels, and the average recovery was found to be 93.95% for isoorientin, 95.30% for isovitexin, 99.79% for orientin, and 100.46% for vitexin. The proposed HPTLC method for estimation of isoorientin, isovitexin, orientin, and vitexin was found to be simple, precise, specific, and accurate and can be used for manufacturing QC of bamboo-leaf flavonoids or for governmental regulatory purposes.

scholarly journals Development and Validation of Stability-Indicating HPLC Method for the Simultaneous Determination of Paracetamol, Tizanidine, and Diclofenac in Pharmaceuticals and Human Serum

2011 ◽  
Vol 94 (1) ◽  
pp. 150-158 ◽  
Author(s):  
Farhan Ahmed Siddiqui ◽  
M Saeed Arayne ◽  
Najma Sultana ◽  
Faiza Qureshi
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Ammonium Carbonate ◽  
Linear Regression Analysis ◽  
Pharmaceutical Formulations ◽  
Analysis Data ◽  
Hplc Method ◽  
Hplc Separation ◽  
Development And Validation

Abstract A method is described for the simultaneous determination of paracetamol, tizanidine, and diclofenac in mixtures. The method was based on HPLC separation of the three drugs followed by UV detection at 254 nm. The separation was carried out on a Hypersil ODS, C18 (250 × 4.6 mm id, 10 μm particle size) column using the mobile phase aqueous 0.2% ammonium carbonate–methanol (60 + 40, v/v) at a flow rate of 1 mL/min. The linear regression analysis data were used for the regression curve in the range of 170–10,000 ng/mL for paracetamol, 120–10,000 ng/mL for tizanidine, and 20–10,000 ng/mL for diclofenac. No chromatographic interference from tablet excipients was found. In order to check the selectivity of the proposed method, degradation studies were carried out using hydrolysis (acid, basic, and neutral), thermolysis, and oxidation. The developed method, after being validated in terms of precision, robustness, recovery, LOD, and LOQ, was successively applied to the analysis of pharmaceutical formulations and human serum.

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