Evaluation of EnSeal®, an adaptive bipolar electrosurgical tissue-sealing device

2012 ◽  
Vol 60 (1) ◽  
pp. 27-40 ◽  
Author(s):  
Miklós Dunay ◽  
Csaba Jakab ◽  
Tibor Németh
Keyword(s):  
Thermal Injury ◽  
Histochemical Technique ◽  
Tetrazolium Chloride ◽  
Limit Values ◽  
The Mean ◽  
Sealing Device ◽  
Tissue Sealing ◽  
Different Tissues ◽  
Enzyme Histochemical Technique ◽  
Experimental Pigs

Relatively few, and inconsistent, data are available in the literature about the properties of EnSeal®, an electrosurgical tissue-sealing device. For this reason, we conducted control safety tests on experimental pigs. The mean burst pressure of sealed vessels (2–7 mm in diameter) proved to be 873.89 ± 120.57 mmHg (n = 60). Surface temperature increased to 69.25 ± 0.98 °C in average (n = 22). The mean diameter of the collateral microscopic thermal injury zone was found to be 0.28 ± 0.04 mm, and it did not show significant differences among the groups of tissues studied (n = 183). During our studies, the device worked reliably and met the relevant requirements in all cases. It can be established that EnSeal® enables high-safety clinical interventions at high blood pressure values, in different tissues and even at sites adjacent to heat-sensitive tissues, and thus it paves the way for new operative solutions in both human and veterinary surgery. In our opinion, the discrepancies between data reported in the literature arise from differences in the design of studies and in the designated limit values. To ensure standardisation, we recommend the use of the nitroblue-tetrazolium chloride/lactate dehydrogenase (NBTC/LDH) enzyme histochemical technique for studying thermal injury induced by the different performance levels and application times of devices operating with electromagnetic energy.

scholarly journals Potential Fertilization Capacity of Two Grapevine Varieties: Effects on Agricultural Production in Designation of Origin Areas in the Northwestern Iberian Peninsula

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 961
Author(s):  
J. Antonio Cortiñas Rodríguez ◽  
María Fernández-González ◽  
Estefanía González-Fernández ◽  
Rosa A. Vázquez-Ruiz ◽  
F. Javier Rodríguez-Rajo ◽  
...  
Keyword(s):  
Airborne Pollen ◽  
Germination Rate ◽  
Pollen Viability ◽  
Weather Conditions ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
The Mean ◽  
Study Sites ◽  
Fertilization Capacity

In the present study, we analyzed the main parameters related with the potential fertilization ability of two grapevine varieties, Godello and Mencía, during the years 2017 and 2018. The research was carried out in two vineyards of the Galician winegrowing Designation of Origin areas of Ribeiro and Ribeira Sacra. Ten vines of each variety were selected for bunch and flower counting, pollen calculations, pollen viability studies by means of aceto-carmine (AC) stain and 2, 3, 5-triphenyl tetrazolium chloride (TTC) methods, and the determination of their germination rate. In all vineyards the 50% fruitset was reached, except for Godello in Cenlle during 2017. The mean coulure value was higher for Godello (40.5%) than for Mencía (31%). Analyzing the pollen production per plant and airborne pollen levels, we observed important discordances between them, which can be due to the influence of weather conditions and be related with self-pollination processes. We found important differences on pollen viability depending on the applied method and variety, with higher values for the AC method than the TTC for both varieties in all study plots, and higher values for Mencía variety than Godello. Regarding germination rates, we observed a marked reduction in 2017 with respect to 2018, in all study sites and for both varieties. The analyzed parameters were useful to explain the different productive abilities of Godello and Mencía varieties in the two studied bioclimatic regions of Ribeiro and Ribeira Sacra.

scholarly journals Composition and Protein Precipitation Capacity of Condensed Tannins in Purple Prairie Clover (Dalea purpurea Vent.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Qianqian Huang ◽  
Tianming Hu ◽  
Zhongjun Xu ◽  
Long Jin ◽  
Tim A. McAllister ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Quantum Coherence ◽  
High Performance ◽  
Degree Of Polymerization ◽  
Protein Precipitation ◽  
Early Flowering ◽  
Plant Tissues ◽  
Heteronuclear Single Quantum Coherence ◽  
The Mean ◽  
Different Tissues

This study aimed to determine the concentration and composition of condensed tannins (CT) in different tissues of purple prairie clover (PPC; Dalea purpurea Vent.) at different maturities and to determine their protein-precipitating capacity. The compositions of CT were elucidated after thiolysis with benzyl mercaptan followed by high-performance liquid-chromatography (HPLC) and 1H–13C heteronuclear single quantum coherence (HSQC) NMR spectroscopy. The results indicated that PPC flowering heads contained the highest CT concentration. Purple prairie clover CT consisted mainly of epicatechin (EC) and epigallocatechin (EGC) subunits. CT in the leaves were composed of more EC and less EGC than CT in stems and flowering heads at both the early flowering (EF) and late flowering (LF) head stages. The mean degree of polymerization was the highest for CT in stems and increased with maturity. CT isolated from PPC leaves at the early flowering head stage exhibited the greatest biological activity in terms of protein precipitation. Overall, the CT in PPC were predominantly procyanidins and the concentration and composition varied among the plant tissues and with maturity.

scholarly journals Using prepucial mucous to cover neourethra in TIP urethroplasty: 4 years experience in a tertiary paediatric hospital of Bangladesh

2020 ◽  
pp. 37-40
Author(s):  
Sabbir Karim ◽  
◽  
Monoarul Islam Talukder ◽  
Sultana Parvin ◽  
◽  
...  
Keyword(s):  
Conflict Of Interest ◽  
Wide Spectrum ◽  
Urethrocutaneous Fistula ◽  
Urethral Plate ◽  
Paediatric Hospital ◽  
Tubularized Incised Plate ◽  
Dartos Flap ◽  
Key Words Hypospadias ◽  
The Mean ◽  
Different Tissues

Background. Hypospadias has a wide spectrum of penile abnormality requiring surgical correction. Most of the cases are of anterior variety and the surgical technique depends on constructing a neo urethra. Snodgrass or Tubularized Incised Plate (TIP) urethroplasty is one of the most popular techniques of urethroplasty which depends on the existing healthy, adequate and intact urethral plate. Different tissues have been described to cover the neourethra as second layer in literature. In this study we modified the TIP urethroplasty by covering the neourethra with 2 layers of pedicled prepucial mucosa or Dartos flap. Objective. To see the outcome of modified Snodgrass or TIP urethroplasty in anterior mid and proximal penile hypospadias. Materials and method. Total 88 patients were operated by modified Snodgrass technique or modified TIP urethroplasty from January 2012 to July 2016. Coronal, subcoronal, distal penile, mid penile and proximal penile hypospadias were included in the study. Every patient underwent modified TIP urethroplasty and outcomes were assessed. Results. The mean age of the patients were 28.34±14.98 months, ranges from 6.5 to 65 months. 9 (10.2%) patients of our series develop urethrocutaneous fistula, 5 (5.68%) patients develop meatal stenosis and 1 (1.1%) patient developed neourethral stricture. Conclusion. TIP urethroplasty is a versatile method to correct penile hypospadias. Covering of the neourethra with prepucial mucosa secure the neourethral anastomosis. The author declares that there is no conflict of interest. Key words: Hypospadias, TIP urethroplasty, Prepucial mucosa.

M1491: Flexible Endoscopic Mucomyotomy of Zenker's Diverticulum Using a Tissue Sealing Device

2010 ◽  
Vol 71 (5) ◽  
pp. AB235
Author(s):  
Diego Juzgado ◽  
Enric Brullet ◽  
Manuel Perez-Miranda ◽  
Francisco Igea ◽  
Carlos Dolz ◽  
...  
Keyword(s):  
Zenker’S Diverticulum ◽  
Zenker's Diverticulum ◽  
Sealing Device ◽  
Tissue Sealing

scholarly journals Some Problems Connected with the Calculation of Stop Loss Premiums for Large Portfolios

1967 ◽  
Vol 4 (2) ◽  
pp. 170-174 ◽  
Author(s):  
Fredrik Esscher
Keyword(s):  
Distribution Function ◽  
Expected Number ◽  
Limit Values ◽  
Empirical Determination ◽  
The Mean ◽  
Premium Rates ◽  
Image Position ◽  
Stop Loss ◽  
The Given

When experience is insufficient to permit a direct empirical determination of the premium rates of a Stop Loss Cover, we have to fall back upon mathematical models from the theory of probability—especially the collective theory of risk—and upon such assumptions as may be considered reasonable.The paper deals with some problems connected with such calculations of Stop Loss premiums for a portfolio consisting of non-life insurances. The portfolio was so large that the values of the premium rates and other quantities required could be approximated by their limit values, obtained according to theory when the expected number of claims tends to infinity.The calculations were based on the following assumptions.Let F(x, t) denote the probability that the total amount of claims paid during a given period of time is ≤ x when the expected number of claims during the same period increases from o to t. The net premium II (x, t) for a Stop Loss reinsurance covering the amount by which the total amount of claims paid during this period may exceed x, is defined by the formula and the variance of the amount (z—x) to be paid on account of the Stop Loss Cover, by the formula As to the distribution function F(x, t) it is assumed that wherePn(t) is the probability that n claims have occurred during the given period, when the expected number of claims increases from o to t,V(x) is the distribution function of the claims, giving the conditioned probability that the amount of a claim is ≤ x when it is known that a claim has occurred, andVn*(x) is the nth convolution of the function V(x) with itself.V(x) is supposed to be normalized so that the mean = I.

scholarly journals A Simple Enzyme Histochemical Method for the Simultaneous Demonstration of Acetylcholinesterase and Monoamine Oxidase in Fixed-Frozen Sections

1997 ◽  
Vol 45 (6) ◽  
pp. 895-902 ◽  
Author(s):  
Daniel D. Dunning ◽  
John G. McHaffie ◽  
Barry E. Stein
Keyword(s):  
Monoamine Oxidase ◽  
Histochemical Method ◽  
Frozen Sections ◽  
Histochemical Technique ◽  
Brightfield Microscopy ◽  
The Individual ◽  
Relationship Of ◽  
The Relationship ◽  
Simultaneous Visualization ◽  
Enzyme Histochemical Technique

We describe an enzyme histochemical technique for the simultaneous demonstration of acetylcholinesterase (AChE) and monoamine oxidase (MAO) (Types A, B, or A+B) in fixed-frozen sections. Several regions in the mesencephalon and brainstem were examined for both somatic and neuropil labeling. The results obtained are equivalent or superior to those obtained using previous methods for the individual localization of these enzymes. The simultaneous visualization of AChE and MAO in the same section allows the relationship of the two enzymes to be easily assessed with brightfield microscopy.

scholarly journals Heavy metal contamination of Pleuronectiformes species from Sinop coasts of the Black Sea

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Levent Bat ◽  
Fatih Şahin ◽  
Ayşah Öztekn
Keyword(s):  
Heavy Metals ◽  
Black Sea ◽  
Heavy Metal Contamination ◽  
Scophthalmus Maximus ◽  
Essential Amino Acids ◽  
The Black Sea ◽  
Limit Values ◽  
Fish Samples ◽  
The Mean ◽  
Cu And Zn

In the Turkish Black Sea coasts fish is particularly consumed because of high protein supplies, essential amino acids, vitamin, and mineral content. Fish are exposed to contaminants such as heavy metals in polluted waters. The aims of the study are to determine heavy metals in Pleuronectiformes species from Sinop coasts of the Black Sea and determine the health risks due to the consumption of contaminated fish. Pleuronectiformes species Scophthalmus maximus (Linnaeus, 1758) belonging to Scophthalmidae family, Arnoglossus laterna (Walbaum, 1792) belonging to Bothidae family and Pegusa lascaris (Risso, 1810) belonging to Soleidae family were collected from Sinop coasts of the Black Sea in fishing season of 2016. Heavy metals (Hg, Cd, Pb, Cu and Zn) in edible tissues were determined by ICP-MS (Agilent 7700x). The limit values given by the international and national organizations did not exceed in the muscle tissues of turbot, Mediterranean scald fish and sand sole. Overall Zn was detected in higher concentrations in all species followed by Cu, Pb, Hg and Cd. The results obtained from the analyses of the maximum levels of the metals except Hg were found in turbot. The highest Hg (0.021±0.007 µg g-1 wet wt.) was found in sand sole. However, the high amounts of Cd, Pb, Cu and Zn in turbot were 0.011±0.004, 0.07±0.005,1.32±0.28 and 14±3 µg g-1 wet wt., respectively. The EDIs and EWIs of the metals were estimated taking into account the mean of metal in all fish samples and the mean consumption of fish per day/week for adults. These results are normally significantly lower than the recommended values of FAO/WHO. Estimated HIs of all the considered metals were below the value of 1, therefore the metals in fish samples do not toxic any apparent threat to the population and these fishes are healthy for consumption.Keywords: Scophthalmus maximus, Arnoglossus laterna, Pegusa lascaris, heavy metals, Black Sea

scholarly journals Laparoscopic suture-less herniotomy using tissue-sealing device for paediatric hydrocele

2020 ◽  
Vol 16 (2) ◽  
pp. 111
Author(s):  
Vikesh Agrawal ◽  
Shashikant Verma ◽  
Himanshu Acharya ◽  
Dhananjaya Sharma
Keyword(s):  
Laparoscopic Suture ◽  
Sealing Device ◽  
Tissue Sealing

scholarly journals Inactivation of Different Salmonella enteriditis Phage Types and Safety and Efficacy of Inactivated Products in Chicken

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
A. Akhtar ◽  
M. Hair-Bejo ◽  
Elawad A. Hussein ◽  
Zunita Zakaria
Keyword(s):  
Body Weight ◽  
Clinical Signs ◽  
The Body ◽  
Safety And Efficacy ◽  
Phage Types ◽  
Significant Difference ◽  
Salmonella Enteriditis ◽  
Gross Lesions ◽  
The Mean ◽  
Different Tissues

This study was conducted to inactivate Salmonella enteriditis phage types (SE pt) and to determine the safety and efficacy of inactivated SE pt in chickens. SE pt 1, 3A, 6A, 7, and 35 were inactivated and inoculated (0.20 mL) in 124 chickens divided into 6 groups (CV1, CV3A, CV6A, CV7, CV35, and CV0 as a control). Sampling was conducted on day 14 after inoculation (pi). Eight chickens from each group were separated on day 14 pi for oral challenge with 0.20 mL/chicken (1010 cfu/mL) SE pt 6A and designated CV1C, CV3AC, CV6AC, CV7C, CV35C, and CV0C as control chickens. On days 7 and 14 postchallenge (pc), 4 chickens from every group were sacrificed for sampling. There was no significant difference in the body weight between different groups. In challenged groups, there was no significant association between different tissues and isolation of Salmonella on days 7 and 14 pc. There was significance (p < 0.05) in isolation of Salmonella when CV0C group was compared with other challenged groups. Significance was not observed between different tissues with respect to induction of microscopic changes. Significance was not observed between day 7 pc and day 14 pc with respect to scoring of lesions induced. Clinical signs and gross lesions were also recorded. ELISA was applied. Only in CV3AC group, the mean antibody titer was 1359 on day 14 pc. The conclusion was that inactivated SE pt 3A and 6A were safe and efficacious for protection against Salmonella enteriditis infection in chickens.

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