Negative allometry of docosahexaenoic acid in the fowl lung and pulmonary surfactant phospholipids

2012 ◽  
Vol 63 (2) ◽  
pp. 202-217 ◽  
Author(s):  
A. Szabó ◽  
M. Mézes ◽  
K. Balogh ◽  
R. Romvári ◽  
P. Horn ◽  
...  
Keyword(s):  
Docosahexaenoic Acid ◽  
Pulmonary Surfactant ◽  
Surfactant Phospholipids

Role of c-fos gene in vasoactive intestinal peptide promoted synthesis of pulmonary surfactant phospholipids

2007 ◽  
Vol 140 (3) ◽  
pp. 117-124 ◽  
Author(s):  
Lian Li ◽  
Hua She ◽  
Shao-Jie Yue ◽  
Xiao-Qun Qin ◽  
Cha-Xiang Guan ◽  
...  
Keyword(s):  
Vasoactive Intestinal Peptide ◽  
Pulmonary Surfactant ◽  
Surfactant Phospholipids

Abnormal Lung Surfactant Related to Essential Fatty Acid Deficiency in a Neonate

PEDIATRICS ◽  
1979 ◽  
Vol 63 (6) ◽  
pp. 855-859
Author(s):  
Zvi Friedman ◽  
Abraham Rosenberg
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Essential Fatty Acid ◽  
Acid Composition ◽  
Pulmonary Surfactant ◽  
Lung Surfactant ◽  
Fatty Acid Pattern ◽  
Hyaline Membrane Disease ◽  
Lavage Fluid ◽  
Surfactant Phospholipids

A low-birth-weight infant, suffering from chronic bronchopulmonary dysplasia following hyaline membrane disease and recurrent episodes of necrotizing enterocolitis, developed biochemical evidence of essential fatty acid (EFA) deficiency in the plasma. Fatty acid composition of phosphatidylcholine and phosphatidylglycerol in the lung lavage fluid was abnormal. Plasma changes includcd a decrease in the level of linoleic acid and an increased level of palmitic, palmitoleic, oleic, and 5,8, 11-eicosatrienoic acids, the ratio of 5,8,11-eicosatrienoic acid to arachidonic acid being > 0.4:1. A lower than normal level of palmitic acid and an increased level of palmitoleic and oleic acids were seen in pulmonary surfactant phospholipid components. Upon treatment and recovery from EFA deficiency, the fatty acid pattern both in plasma and surfactant phospholipids returned to normal along with clinical improvement. An association between EFA deficiency and altered fatty acid composition of pulmonary surfactant phospholipids is suggested.

Pulmonary surfactant phospholipids modulate priming of rabbit alveolar macrophages for oxidative responses

1992 ◽  
Vol 51 (4) ◽  
pp. 379-385 ◽  
Author(s):  
Hiroshi Hayakawa ◽  
Girish Giridhar ◽  
Quentin N. Myrvik ◽  
Louis Kucera
Keyword(s):  
Alveolar Macrophages ◽  
Pulmonary Surfactant ◽  
Surfactant Phospholipids ◽  
Oxidative Responses

SP-B refining of pulmonary surfactant phospholipid films

1999 ◽  
Vol 277 (6) ◽  
pp. L1179-L1189 ◽  
Author(s):  
Kaushik Nag ◽  
James G. Munro ◽  
Kevin Inchley ◽  
Samuel Schürch ◽  
Nils O. Petersen ◽  
...  
Keyword(s):  
Pulmonary Surfactant ◽  
Film Formation ◽  
Surfactant Protein ◽  
Neutral System ◽  
Area Reduction ◽  
Surfactant Protein B ◽  
Squeeze Out ◽  
Planar Films ◽  
Surfactant Phospholipids ◽  
Film Area

Pulmonary surfactant stabilizes the alveoli by lining the air-fluid interface with films that reduce surface tension to near 0 mN/m (γmin). Surfactant protein B (SP-B) enhances the surface activity of surfactant phospholipids. A captive bubble tensiometer (CBT) was used to study the properties of adsorbed films of dipalmitoylphosphatidylcholine (DPPC) with acidic 1-palmitoyl-2-oleoyl- sn-glycero-3-[phospho- rac-(1-glycerol)] (POPG) or neutral 1-palmitoyl-2-oleoyl- sn-glycerol-3-phosphocholine with (7:3) and without 1% dimeric SP-B. SP-B enhanced the adsorption rate of DPPC-containing neutral or acidic lipid suspensions (1 mg/ml) to a similar extent. Quasi-static cycling of these films revealed that SP-B significantly decreased the film area reduction required to reach γmin for the acidic but not for the neutral system. The results obtained with DPPC-phosphatidylglycerol (PG)-SP-B were consistent with selective DPPC adsorption into the surface monolayer during film formation. Film area reduction required to reach γmin with this system (with and without calcium) approached that of pure DPPC, suggesting selective DPPC insertion and PG squeeze-out. Dynamic cycling of such films showed that larger film area reductions were required to reach γmin for the neutral than for acidic system, even after 20 cycles. Fluorescence microscopy of solvent-spread DPPC-POPG-SP-B planar films revealed highly condensed structures at ∼25 mN/m, although no specific PG phase-segregated structures could be identified. The study suggests that specific interactions of SP-B with acidic phospholipids of surfactant may be involved in the generation and maintenance of DPPC-rich films in the alveoli.

Altered fatty acid composition of lung surfactant phospholipids in interstitial lung disease

2002 ◽  
Vol 283 (5) ◽  
pp. L1079-L1085 ◽  
Author(s):  
R. Schmidt ◽  
U. Meier ◽  
P. Markart ◽  
F. Grimminger ◽  
H. G. Velcovsky ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Interstitial Lung Disease ◽  
Palmitic Acid ◽  
Lung Disease ◽  
Pulmonary Surfactant ◽  
Hypersensitivity Pneumonitis ◽  
Lung Surfactant ◽  
Relative Content ◽  
Surfactant Aggregates ◽  
Surfactant Phospholipids

Deterioration of pulmonary surfactant function has been reported in interstitial lung disease; however, the molecular basis is presently unclear. We analyzed fatty acid (FA) profiles of several surfactant phospholipid classes isolated from large-surfactant aggregates of patients with idiopathic pulmonary fibrosis (IPF; n = 12), hypersensitivity pneumonitis ( n = 5), and sarcoidosis ( n = 12). Eight healthy individuals served as controls. The relative content of palmitic acid in phosphatidylcholine was significantly reduced in IPF (66.8 ± 2.5%; means ± SE; P < 0.01) but not in hypersensitivity pneumonitis (78.5 ± 1.8%) and sarcoidosis (78.2 ± 3.1%; control 80.1 ± 0.7%). In addition, the phosphatidylglycerol FA profile was significantly altered in the IPF patients, with a lower relative content of its major FA, oleic acid, at the expense of saturated FA. In the phosphatidylcholine class, a significant correlation between the impairment of biophysical surfactant function and decreased percentages of palmitic acid was noted. We conclude that significant alterations in the FA profile of pulmonary surfactant phospholipids occur predominantly in IPF and may contribute to the disturbances of alveolar surface activity in this disease.

Uptake of liposomal phosphatidylcholine by granular pneumocytes in primary culture

1983 ◽  
Vol 245 (5) ◽  
pp. C397-C404 ◽  
Author(s):  
A. Chander ◽  
W. D. Claypool ◽  
J. F. Strauss ◽  
A. B. Fisher
Keyword(s):  
Fatty Acids ◽  
Oxidative Phosphorylation ◽  
Primary Culture ◽  
Pulmonary Surfactant ◽  
Neutral Lipids ◽  
Physiological Role ◽  
Cell Protein ◽  
Surface Binding ◽  
Metabolic Degradation ◽  
Surfactant Phospholipids

Reuptake of pulmonary surfactant phospholipids was investigated with rat granular pneumocytes in primary culture and L-alpha-[2-palmitoyl-9,10-3H]dipalmitoyl phosphatidylcholine:egg phosphatidylcholine:phosphatidylglycerol:cholesterol (10:5:2:3, mol/mol) liposomes. Uptake of liposomal phosphatidylcholine by granular pneumocytes increased with time and concentration of phosphatidylcholine in the medium. With 150 microM phosphatidylcholine, uptake was about 5 nmol/mg cell protein in 2 h. Phosphatidylcholine uptake was in large part due to net transfer of vesicles as indicated by uptake of [14C]sucrose encapsulated in the aqueous compartment of liposomes. Using dipalmitoyl phosphatidylcholine:cholesterol (1:1) liposomes, uptake was inhibited significantly at 26 degrees C and completely at 4 degrees C. Inhibitors and uncouplers of oxidative phosphorylation had no effect on uptake although uptake was somewhat inhibited in the presence of either 5.6 mM 2-deoxy-D-glucose or 10 mM sodium fluoride. Cell-associated lipid radioactivity decreased after treatment with 0.25% trypsin. The percent radioactivity that was trypsin releasable decreased with increasing time and phosphatidylcholine concentration. The results suggest that uptake of phospholipids by these cells is by surface binding followed by internalization. After 2 h of incubation, 65.3 +/- 3.1% of the cell-associated radioactivity was present in phosphatidylcholine, a small fraction in phosphatidylglycerol, and the remainder in lysophosphatidylcholine, free fatty acids, and other neutral lipids, suggesting metabolic degradation of internalized lipids. This process of phospholipid uptake and degradation may have a physiological role in metabolism of surfactant phospholipids in the lung.

scholarly journals The Collapse of Monolayers Containing Pulmonary Surfactant Phospholipids Is Kinetically Determined

2005 ◽  
Vol 89 (1) ◽  
pp. 306-314 ◽  
Author(s):  
Wenfei Yan ◽  
Barbora Piknova ◽  
Stephen B. Hall
Keyword(s):  
Pulmonary Surfactant ◽  
Surfactant Phospholipids
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