scholarly journals Optimizing sample size for molecular characterization of open-pollinated maize (Zea mays L.) varieties using simple sequence repeat markers

2018 ◽  
Vol 46 (4) ◽  
pp. 569-579 ◽  
Author(s):  
G. Arafayne ◽  
A. Menkir ◽  
V.O. Adetimirin ◽  
M. Gedil
Keyword(s):  
Zea Mays ◽  
Sample Size ◽  
Molecular Characterization ◽  
Simple Sequence Repeat ◽  
Zea Mays L ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Simple Sequence

scholarly journals Molecular Characterization of Moroccan Fig Germplasm Using Intersimple Sequence Repeat and Simple Sequence Repeat Markers To Establish A Reference Collection

HortScience ◽  
2005 ◽  
Vol 40 (1) ◽  
pp. 29-32 ◽  
Author(s):  
B. Khadari ◽  
A. Oukabli ◽  
M. Ater ◽  
A. Mamouni ◽  
J.P. Roger ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Simple Sequence Repeat ◽  
Germplasm Collection ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Reference Collection ◽  
Simple Sequence

A study was conducted to identify genotypes present in a Moroccan fig germplasm collection and provide the first database for a reference collection in northern Morocco. In total, 75 fig samples were analyzed using 8 intersimple sequence repeat primers and 6 simple sequence repeat loci. From these samples, we identified 72 fig genotypes. In genetically heterogeneous cultivars, genotypes under the same denomination were distinguished by both molecular markers and pomological traits. Molecular analysis was used to classify the germplasm into 46 well-defined cultivars and 6 caprifig trees. The remaining genotypes were not clearly identified due to three cases of mislabeling and four cases of homonymy. No evidence was found for the occurrence of geographically widespread genotypes.

scholarly journals Development and characterization of non-coding RNA based simple sequence repeat markers in Capsicum species

Genomics ◽  
2020 ◽  
Vol 112 (2) ◽  
pp. 1554-1564 ◽  
Author(s):  
Vandana Jaiswal ◽  
Abdul Rawoof ◽  
Meenakshi Dubey ◽  
Sushil Satish Chhapekar ◽  
Vineet Sharma ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Non Coding Rna ◽  
Capsicum Species ◽  
Simple Sequence

Characterization of expressed sequence tag-derived simple sequence repeat markers for 17 Linum species

Botany ◽  
2010 ◽  
Vol 88 (5) ◽  
pp. 537-543 ◽  
Author(s):  
Yong-Bi Fu ◽  
Gregory W. Peterson
Keyword(s):  
Simple Sequence Repeat ◽  
Expressed Sequence Tag ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Linum Usitatissimum L ◽  
Evolutionary Studies ◽  
Expressed Sequence ◽  
Simple Sequence ◽  
Cultivated Flax

One major challenge in genetic and evolutionary studies of wild flax species is the lack of informative molecular markers. A set of 100 informative expressed sequence tag-derived simple sequence repeat (EST-SSR) primer pairs developed in cultivated flax ( Linum usitatissimum L.) were characterized on 35 Linum accessions representing 17 Linum species for their transferability to other Linum species. Ninety-nine primer pairs displayed scorable polymorphisms across 35 Linum samples and generated 627 bands likely from 121 loci. About 50% of the detected bands occurred only in three or fewer samples. A total of 393 bands, likely from 116 loci, were detected by 97 primer pairs in Linum bienne Mill. samples, but only up to 60 bands, likely from up to 39 loci, were revealed by 6 to 37 primer pairs in the samples of the other 15 Linum species. The L. bienne samples displayed 23.7% more EST-SSR variation than the L. usitatissimum samples. These characterized EST-SSR markers should be useful for future genetic diversity and evolutionary studies of Linum species, particularly for the progenitor of cultivated flax.

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