scholarly journals Effect of two low doses of prostaglandin F2α on luteolysis in dairy cows

2017 ◽  
Vol 65 (1) ◽  
pp. 105-114 ◽  
Author(s):  
Tsung-Ching Liu ◽  
Chia-Yu Ho ◽  
Jacky Peng-Wen Chan
Keyword(s):  
Dairy Cows ◽  
Standard Dose ◽  
Prostaglandin F2α ◽  
Low Doses ◽  
Serum Progesterone ◽  
Modified Method ◽  
Dose Method ◽  
Prostaglandin F ◽  
Preliminary Study ◽  
Larger Sample

In this preliminary study, we determined the effect of a modified method involving the administration of two low doses of prostaglandin F2α (PGF2α) at an interval of 24 h on luteolysis in dairy cows, and compared it with the standard single-dose method. Twenty-six cows were assigned to three groups treated with two low doses (TLD group, n = 10), one standard dose (SD group, n = 10), and one low dose (OLD group, n = 6) on day 9 to 10 of the oestrous cycle (day 0 = the day of PGF2α administration). Their serum progesterone (P4) levels and corpus luteum (CL) sizes were measured daily from day 0 to 4 to assess CL regression. The results indicated that the proportion of complete luteolysis, indicating a P4 value ≤ 1 ng/mL on day 3, was higher in the TLD group (100.0%) than in the SD (60.0%) and OLD (66.7%) groups. Ultrasonically detected changes in the CL area correlated with the shifts in the P4 values in both the TLD and the SD groups. The remaining CL area was significantly smaller in the TLD group (17.8% ± 3.3%) than in the SD or OLD group on day 4. Thus, we concluded that the proportion of luteolysis in cows was increased with two low doses of PGF2α as compared to a single PGF2α dose, indicating the necessity of the second dose of PGF2α. However, further studies with larger sample sizes in the field are required.

PROLACTIN AND THE REGULATION OF 20α-DIHYDROPROGESTERONE SECRETION OF THE SUPER-LUTEINIZED RAT OVARY DURING LUTEOLYSIS INDUCED BY A PROSTAGLANDIN F2α ANALOGUE

1978 ◽  
Vol 87 (3) ◽  
pp. 625-631 ◽  
Author(s):  
P. A. Torjesen ◽  
R. Dahlin ◽  
E. Haug ◽  
A. Aakvaag
Keyword(s):  
Prostaglandin F2α ◽  
Serum Levels ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
Serum Progesterone ◽  
Prostaglandin F ◽  
Rat Ovary ◽  
Pre Treatment ◽  
Observation Period

ABSTRACT Twenty-five day old female rats were treated with pregnant mare's serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) to achieve a state of luteinization. Eight days after the HCG administration luteolysis was induced by a subcutaneous injection of 5 μg of the prostaglandin F2α analogue cloprostenol (Estrumate®, ICI 80996). In animals treated with 2-Br-α-ergocryptine (BEC), administration of cloprostenol decreased serum progesterone levels from 580 to 20 ng/ml in 5 h and progesterone remained low for the next 18 h. The serum levels of 20α-dihydroprogesterone (20α-DHP) and prolactin (PRL) remained at pre-treatment values (20α-DHP 85 to 170 ng/ml; PRL less than 5 ng/ml) throughout the observation period. When animals treated with both BEC and cloprostenol were given PRL 5 h after the prostaglandin injection, an increased 20α-DHP level (630 ng/ml) was found 23 h after the cloprostenol administration, while the progesterone level was decreased (70 ng/ml). These findings were similar to the observations following cloprostenol treatment alone. The study indicates a causal relationship between the increase in the serum levels of PRL and 20α-DHP observed after PGF2α induced luteolysis in rats with superluteinized ovaries.

7 OESTRAL RESPONSE AND FERTILITY IN DAIRY COWS TREATED WITH PROGESTERONE FOR 6 DAYS PRIOR TO OESTRUS SYNCHRONIZATION WITH PROSTAGLANDIN F2α

2014 ◽  
Vol 26 (1) ◽  
pp. 118
Author(s):  
M. Orozco ◽  
C. G. Gutierrez ◽  
R. Lopez ◽  
C. Aguilar ◽  
C. Roque ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Prostaglandin F2α ◽  
Control Group ◽  
Serum Progesterone ◽  
Progesterone Treatment ◽  
The Individual ◽  
Rectal Palpation ◽  
And Control ◽  
Intravaginal Device

The present study tested whether the increase in serum progesterone concentrations for 6 days before oestrus synchronization with prostaglandin F2α (PGF2) increases oestral response, pregnancy rate, and reduces the incidence of twin births in dairy cattle. Seven hundred and eighty-three first-service dairy cows were synchronized with 2 injections of PGF2 14 days apart, starting on Day 35 postpartum. Six days before the second PGF2 injection, cows were assigned to 2 groups: P4 and control. The P4 group (n = 387) received a progesterone-releasing intravaginal device and an intramuscular injection of 500 mg of progesterone. The control group (n = 396) did not receive the progesterone-releasing intravaginal device or progesterone injection. Cows were inseminated 12 h after exhibiting oestrus. Pregnancy diagnosis was performed 40 and 45 days after insemination by rectal palpation and the cumulative pregnancy rate on Day 90 postpartum was determined retrospectively through the individual records. The response to the progesterone treatment varied according to the parity of the cows (P < 0.05). Thus, progesterone administration improved the percentage of cows detected in oestrus in multiparous [192/255 (75.2%) v. 161/267 (60.2%)] but not in primiparous [93/132 (70.4%) v. 90/129 (69.7%)] cows. Progesterone treatment increased pregnancy rate in multiparous [53/192 (27.6%) v. 27/161 (16.7%)] but not in primiparous cows [25/93 (26.8%) v. 29/90 (32.2%)]. Likewise, progesterone administration increased the cumulative percentage of pregnant cows on day 90 postpartum in multiparous [149/255 (58.4%) v. 129/267 (48.3%)] but not in primiparous cows [77/132 (58.3%) v. 82/129 (63.5%)]. The incidence of twin births was lower (P = 0.07) in cows treated with progesterone [1/74 (1%)] than in the control group [4/53 (7%)]. It is concluded that progesterone administration before oestrus synchronization with PGF2 in first service dairy cows improves oestral response, increases pregnancy rate, and percentage of pregnant animals on Day 90 postpartum in multiparous cows, but not in primiparous cows, and decreases the incidence of twin births. This research was supported by PAPIIT-UNAM research grant (IN219811-3).

THE EFFECTS OF TIME OF INSEMINATION ON FERTILITY IN BEEF HEIFERS SYNCHRONIZED WITH PROSTAGLANDIN F2α

1977 ◽  
Vol 57 (1) ◽  
pp. 47-51 ◽  
Author(s):  
J. G. MANNS ◽  
M. S. WENKOFF ◽  
W. M. ADAMS ◽  
G. RICHARDSON
Keyword(s):  
Prostaglandin F2α ◽  
Corpora Lutea ◽  
Beef Heifers ◽  
Serum Progesterone ◽  
Group 4 ◽  
Prostaglandin F ◽  
Group 3 ◽  
Group 2 ◽  
Observation Period ◽  
Group 1

Two injections of prostaglandin F2α (PGF2α) spaced 12 days apart were used to synchronize estrus in Hereford heifers. Animals were inseminated at 75 h (group 2), 80 h (group 3) or 85 h (group 4) after the second injection of PGF2α. Untreated control animals (group 1) were inseminated as detected in estrus over an observation period of approximately 35 days. Immediately before, and 24 h after each PGF2α injection, blood was collected for progesterone assay. Fertility expressed as calving rates was as follows: group 1, 33/77 (43%); group 2, 30/79 (38%); group 3, 29/79 (37%); group 4, 20/73 (27%). Fertility was depressed at 85 h vs. control (P < 0.05) in PGF2α-treated animals but there were no other significant differences. Progesterone assays showed that 65% of animals had progesterone-secreting corpora lutea at the first injection of PGF2α. There was no relationship between fertility and either serum progesterone concentration or the day of the cycle at the second injection of PGF2α.

scholarly journals Effects of Intrauterine Infusion of a Chitosan Solution on Recovery and Subsequent Reproductive Performance of Early Postpartum Dairy Cows with Endometritis: A Pilot Field Trial

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 197
Author(s):  
Hiroaki Okawa ◽  
Missaka M.P. Wijayagunawardane ◽  
Peter L.A.M. Vos ◽  
Osamu Yamato ◽  
Masayasu Taniguchi ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Reproductive Performance ◽  
Polymorphonuclear Leukocytes ◽  
Prostaglandin F2α ◽  
Chitosan Solution ◽  
Control Group ◽  
Control Groups ◽  
Early Postpartum Period ◽  
Early Postpartum ◽  
And Control

This study investigated the efficacy of intrauterine infusion of a chitosan solution (CHT) on uterine recovery in early postpartum dairy cows with or without endometritis, and their subsequent reproductive performance. In Experiment 1, cows with endometritis at 3 weeks postpartum were administered CHT (n = 5) and prostaglandin F2α (PGF2α) (n = 4). Untreated cows (n = 7) served as the control group. In Experiment 2, 18 cows with a normally recovered uterus at the fresh cow check (mean, 35 days postpartum) were assigned to the CHT (n = 10) and control (n = 8) groups, and intrauterine infusion was conducted in the CHT group. Overall, in Experiment 1, the percentage of polymorphonuclear leukocytes significantly declined in the CHT group (32.3 ± 10.2 to 5.5 ± 2.4, p < 0.05) from week 3 to week 5, but no decline occurred in the PGF2α and control groups. In Experiment 2, the CHT and control groups showed no significant differences in reproductive parameters, suggesting the absence of adverse effects of CHT on fertility. These results suggest that intrauterine infusion of CHT in the early postpartum period effectively accelerates uterine recovery from endometritis and might be a suitable replacement for PGF2α administration.

Effects of body weight loss on serum progesterone concentrations of non-lactating dairy cows

2011 ◽  
Vol 75 (1) ◽  
pp. 131-137 ◽  
Author(s):  
R.O. Rodrigues ◽  
C. Trevisanuto ◽  
R.F. Cooke ◽  
J.L.M. Vasconcelos
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
Dairy Cows ◽  
Body Weight Loss ◽  
Serum Progesterone ◽  
Lactating Dairy Cows

Expression of prostaglandin G/H synthase (PGHS) and heat shock protein-70 (HSP-70) in the corpus luteum (CL) of prostaglandin F2α-treated immature superovulated rats

2004 ◽  
Vol 82 (6) ◽  
pp. 363-371 ◽  
Author(s):  
R M Narayansingh ◽  
M Senchyna ◽  
M M Vijayan ◽  
J C Carlson
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Corpus Luteum ◽  
Heat Shock Protein 70 ◽  
Prostaglandin F2α ◽  
Sampling Period ◽  
Immunoblot Analysis ◽  
Hsp 70 ◽  
Prostaglandin F

In this study we examined the mechanism of corpus luteum (CL) regression by measuring changes in expression of prostaglandin G/H synthase-1 (PGHS-1) and -2 (PGHS-2) in day 4 CL and inducible heat shock protein 70 (HSP-70) in day 4 and day 9 CL of immature superovulated rats. The rats were superovulated and treated with 500 µg of prostaglandin F2α (PGF2α) on day 4 or day 9 after CL formation. Ovaries and serial blood samples were removed during the 24-hour period following treatment. Plasma progesterone was determined by radioimmunoassay while mRNA abundance and protein expression were assessed by semiquantitative RT-PCR and immunoblot analysis, respectively. One hour after PGF2α, both day 4 and day 9 rats exhibited a significant decrease in progesterone secretion; however, there was a greater decrease in day 9 rats. In ovarian samples removed on day 4, there was a significant increase in mRNA for PGHS-2 at 1 hour after PGF2α. PGHS-1 mRNA content remained unchanged. Immunoblot analyses showed an increase in PGHS-2 protein expression only at 8 h. There were no changes in PGHS-1 protein expression. In day 9 rats, ovarian HSP-70 protein levels increased by 50% after PGF2α injection; however, on day 4 there was no change in expression of this protein over the sampling period. These results suggest that expression of PGHS-2 may be involved in inhibiting progesterone production and that expression of HSP-70 may be required for complete CL regression in the rat.Key words: rat, prostaglandin F2α, corpus luteum, prostaglandin G/H synthase, heat shock protein-70.

Effect of gonadotropin-releasing hormone and prostaglandin F2α on reproduction in postpartum dairy cows

1983 ◽  
Vol 19 (6) ◽  
pp. 763-770 ◽  
Author(s):  
G.F. Richardson ◽  
L.F. Archbald ◽  
D.M. Galton ◽  
R.A. Godke
Keyword(s):  
Dairy Cows ◽  
Prostaglandin F2α ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone

Estrous synchronization and fertility of cross-bred dairy cows using prostaglandin F2α and gonadotropin releasing hormone

2021 ◽  
pp. 171-175
Author(s):  
Abubakr O. Ismail ◽  
◽  
Erneo B. Ochi ◽  
Ambrose S. Jubara ◽  
Bakri Ahmed A/Rahim ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Releasing Hormone ◽  
Treatment Groups ◽  
Estrous Synchronization ◽  
Descriptive Statistical Analysis

A comparative hormonal study of prostaglandin, gonadotropin releasing hormone and their combination was conducted on 30 randomly selected cross-bred dairy cows of SEMEX project for artificial insemination in Hillat Kuku, Khartoum North, Sudan. The study attempts to determine the response of the animals to hormonal induction and synchronization of estrous as well as fertility following fixed time artificial insemination. Three treatment groups of 10 cows each were undertaken. Groups 1, 2 and 3 involved administration of prostaglandin F2α (PGF2α), gonadotropin releasing hormone (GnRH) and a combination of PGF2α + GnRH, respectively. Descriptive statistical analysis was performed. The results revealed that all the three protocols induced and synchronized estrous were almost synchronous in all the treated groups. However, protocol 3 revealed a significantly (p<0.05) better result of 70% pregnancy rate compared to other two protocols that equally provided 50% pregnancy rates. The study resolves that protocol 3 be highly recommended to change the mindset of farmers about the spread of reproductive technology in Sudan. Protocols 1 and 2 provided acceptable pregnancy rates that can be enhanced with the improvement of management.

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